The Expression and Significance of Pyroptosis Factor Cytokine Caspase-1、IL-1β and IL-18 in Endometrial Polys
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摘要:
目的 检测细胞焦亡相关因子Caspase-1、IL-1β与IL-18在子宫内膜息肉组织中的表达情况，探讨细胞焦亡与子宫内膜息肉发病的关系，为深入研究子宫内膜息肉的发病机制提供依据。 方法 选取2020年1月至2021年1月在昆明医科大学第一附属医院妇科行宫腔镜探查术 + 诊刮术的60例患者，将术后病检为子宫内膜息肉的30例患者纳入观察组，术后病检为增生期子宫内膜的30例患者纳入对照组。采用免疫组化法检测Caspase-1、IL-1β与IL-18在观察组和对照的表达情况。 结果 Caspase-1主要表达于胞浆内，观察组的IRS得分为：（4.88±1.8）分，对照组的IRS得分为：（1.37±1.13）分，Caspase-1在观察组的表达高于对照组（t = 8.95，P < 0.05）。IL-1β在2组中均不表达。IL-18主要表达于胞浆和胞核内，观察组的IRS得分为：(6.84±2.41)分，对照组的IRS得分为：（1.73±0.90）分，IL-18在观察组的表达高于对照组（t = 10.87，P < 0.05）。 结论 Caspase-1和IL-18在子宫内膜息肉组织中高表达，这两种细胞焦亡相关因子可能参与子宫内膜息肉的发生。 -
关键词:
- 子宫内膜息肉 /
- 细胞焦亡 /
- 半胱氨酸天冬氨酸特异性蛋白酶-1 /
- 白介素-1β /
- 白介素-18
Abstract:Objective To detect the expression of caspase-1、IL-1β and IL-18 in endometrial polyps so as to explore the relationship between pyroptosis and endometrial polyps and provide the basis for exploring the pathogenesis of endometrial polyps. Methods In total 30 endometrial polyps specimens （from the observation group） and 30 normal endometrial specimens （from the control group） were collected from the patients visiting the Obstetrics and Gynecology Department of the First Affiliated Hospital of Kunming Medical University from January 2020 to January 2021. The expression of caspase-1, IL-1β and IL-18 in both groups were detected by immunohistochemical method. Results Caspase-1 was mainly expressed in the cytoplasm, the IRS score in the observation group and control group was 4.88±1.82 points and 1.37±1.13 points respectly, caspase-1 express was higher in the observation group （t = 8.95, P < 0.05）. IL-1β was not expressed in both groups. IL-18 was mainly expressed in cytoplasm and nucleus, the IRS score in the observation group and control group was 6.84±2.41 points and 1.73±0.90 points respectly, IL-18 express was higher in observation group （t = 10.87, P < 0.05）. Conclusion Caspase-1 and IL-18 in endometrial polyps is increased, and these two pyroptosis related factors may be involved in the occurrence of endometrial polyps. -
Key words:
- Endometrial polyps /
- Pyroptosis /
- Caspase-1 /
- IL-1β /
- IL-18
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图 1 Caspase-1在观察组和对照组的表达
a～b：Caspase-1在观察组的表达（a：SP×40；b：SP×200）；c～d：Caspase-1在对照组的表达（c：SP×40；d：SP×200）。
Figure 1. Expression of caspase-1in observation group and control group
图 2 Caspase-1在观察组和对照组的表达
与对照组比较，****P < 0.05。
Figure 2. Expression of caspase-1in observation group and control group
图 3 IL-18在观察组和对照组的表达
a～b：IL-18在观察组的表达（a：SP×40；b：SP×200）；c～d：IL-18在对照组的表达性（c：SP×40；d：SP×200）。
Figure 3. Expression of IL-18 in observation group and control group
图 4 IL-18在观察组和对照组的表达
与对照组比较，**P < 0.05。
Figure 4. Expression of IL-18 in observation group and control group
图 5 IL-1β在观察组和对照组的表达
a～b：IL-1β在观察组的表达（a：SP×40；b：SP×200）；c～d：IL-1β在对照组表达（c：SP×40；d：SP×200）。
Figure 5. Expression of IL-1βin observation group and control group
表 1 患者基本情况（
$\bar x \pm s$ ）Table 1. Basal condition of patients （
$\bar x \pm s$ ）相关因素 观察组
30例对照组
30例t/χ2 P 年龄（岁） 44.33 ± 8.28 45.40 ± 7.17 0.530 0.600 孕次（次） 2.67 ± 1.06 2.73 ± 0.90 0.260 0.800 产次（次） 1.93 ± 0.94 2.27 ± 0.74 1.520 0.130 
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