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RSS2016 Vol. 37, No. 06
2016, 37(06)
Abstract:
［Abstract］Multiple myeloma （MM） is a malignant plasma cell disease， accounting for about 1% of all malignant tumors， is still uncurable， minimal residual disease（MRD） is the reason， therefore，removal of minimal residual disease maybe possible to cure the disease. In a variety of treatment methods， immune gene therapy for multiple myeloma is a hot research topic in recent years， has achieved good results in vitro and in vivo experiments， in this review immune genes therapy in multiple myeloma research would be discussed.
［Abstract］Multiple myeloma （MM） is a malignant plasma cell disease， accounting for about 1% of all malignant tumors， is still uncurable， minimal residual disease（MRD） is the reason， therefore，removal of minimal residual disease maybe possible to cure the disease. In a variety of treatment methods， immune gene therapy for multiple myeloma is a hot research topic in recent years， has achieved good results in vitro and in vivo experiments， in this review immune genes therapy in multiple myeloma research would be discussed.
2016, 37(06)
Abstract:
［Abstract］Objective To explo the antioxidant effect and molecular mechanism of gastrodin（Gas） in epilepsy（EP） rats induced by LiCl-pilocarpine（PILO）. Methods Eighty male SD rats were randomly divided into 5 groups: sham group， EP group， therapy groups（pretreated with 60 mg/kg， 90 mg/kg， 120 mg/kg of gastrodin respectively）. The EP model was esteblished by peritoneal injection of LiCl-PILO. Therapy groups were pretreated with various concerntration of Gas. The control group was given the same dosage of normal saline. The alteration of behavior was observed，the concentration of catalase（CAT），glutathion（GSH）， superoxide dismutase（SOD），glutathion reductase（GR）， total antioxidtion（T-AOC） and malondialdehyde（MDA） in rats brain cortex were detected by chemical colorimetric method， phosphorylation of p38 was determined by western blot. Results There was no EP seizure in sham group，and the EP seizure degree in therapy groups（gas pretreated groups） was significantly decreased，and had statistically significant difference with EP group（P＜0.05）. The EP model rats exhibited a significant decrease in the concentration of endogenous antioxidants（CAT， GSH， SOD， GR and T-AOC），while an increase of the concentration of MDA and phosphorylation p38 protein as compared to sham group（P＜0.05）. After treatment of the Gas，treatment group rats attenuated the seizure degree，exhibited a significant increase of the concentration of endogenous antioxidants（P＜0.05），while a decrease in concentration of MDA and phosphorylation of p38 as compared to model group（P＜0.05）. Conclusion Gas may have a neuroprotective role in central nervous system of epileptic rats modle by down-regulateing the seizure degree and the activity of p38 kinase and up-regulateing the content of endogenous antioxidants.
［Abstract］Objective To explo the antioxidant effect and molecular mechanism of gastrodin（Gas） in epilepsy（EP） rats induced by LiCl-pilocarpine（PILO）. Methods Eighty male SD rats were randomly divided into 5 groups: sham group， EP group， therapy groups（pretreated with 60 mg/kg， 90 mg/kg， 120 mg/kg of gastrodin respectively）. The EP model was esteblished by peritoneal injection of LiCl-PILO. Therapy groups were pretreated with various concerntration of Gas. The control group was given the same dosage of normal saline. The alteration of behavior was observed，the concentration of catalase（CAT），glutathion（GSH）， superoxide dismutase（SOD），glutathion reductase（GR）， total antioxidtion（T-AOC） and malondialdehyde（MDA） in rats brain cortex were detected by chemical colorimetric method， phosphorylation of p38 was determined by western blot. Results There was no EP seizure in sham group，and the EP seizure degree in therapy groups（gas pretreated groups） was significantly decreased，and had statistically significant difference with EP group（P＜0.05）. The EP model rats exhibited a significant decrease in the concentration of endogenous antioxidants（CAT， GSH， SOD， GR and T-AOC），while an increase of the concentration of MDA and phosphorylation p38 protein as compared to sham group（P＜0.05）. After treatment of the Gas，treatment group rats attenuated the seizure degree，exhibited a significant increase of the concentration of endogenous antioxidants（P＜0.05），while a decrease in concentration of MDA and phosphorylation of p38 as compared to model group（P＜0.05）. Conclusion Gas may have a neuroprotective role in central nervous system of epileptic rats modle by down-regulateing the seizure degree and the activity of p38 kinase and up-regulateing the content of endogenous antioxidants.
2016, 37(06)
Abstract:
［Abstract］Objective To investigate the effect of geraniin on expression of Wnt3a protein and mRNA in bone marrow stromal cell （BMSC） from osteoporotic rats. Methods The model of osteoporosis （OP） was duplicated by ovariectomy in rats. BMSCs were isolated and cultured. BMSCs from shamed rats were routinly cultured and taken as normal control， and BMSCs from OP rats were divided into model group， 1 μmol/L simvastatin positive group， and geraniin group （0.01， 0.1， 1， 10， 100 μmol/L）， respectively. The methods of realtime-PCR and western blot were used to assay the protein and mRNA expression of wnt3a， respectively．Results As compared with normal control group， the protein and mRNA expression of wnt3a in model group were significantly suppressed; Compared with model group， 1 μmol/L simvastatin， and 0.1， 1， 10 and 100 μmol/L geraniin significantly increased the expression of wnt3a protein and mRNA. Conclusion It is suggested that geraniin activates wnt/β-catenin pathway though increasing the expression of signaling protein wnt 3a in BMSCs from OP rats. It would be beneficial to osteogenic differentiation of BMSCs and osteogenesis.
［Abstract］Objective To investigate the effect of geraniin on expression of Wnt3a protein and mRNA in bone marrow stromal cell （BMSC） from osteoporotic rats. Methods The model of osteoporosis （OP） was duplicated by ovariectomy in rats. BMSCs were isolated and cultured. BMSCs from shamed rats were routinly cultured and taken as normal control， and BMSCs from OP rats were divided into model group， 1 μmol/L simvastatin positive group， and geraniin group （0.01， 0.1， 1， 10， 100 μmol/L）， respectively. The methods of realtime-PCR and western blot were used to assay the protein and mRNA expression of wnt3a， respectively．Results As compared with normal control group， the protein and mRNA expression of wnt3a in model group were significantly suppressed; Compared with model group， 1 μmol/L simvastatin， and 0.1， 1， 10 and 100 μmol/L geraniin significantly increased the expression of wnt3a protein and mRNA. Conclusion It is suggested that geraniin activates wnt/β-catenin pathway though increasing the expression of signaling protein wnt 3a in BMSCs from OP rats. It would be beneficial to osteogenic differentiation of BMSCs and osteogenesis.
2016, 37(06)
Abstract:
［Abstract］Objective To investigate the molecular mechanism of Loureirin A mediated anti-hepatic fibrosis by evaluting its effects on proliferation ，secretion of α-smooth muscle actin（α-SMA）and transforming growth factor-beta1（TGF-β1），and expression of rat hepatic stellate cells in vitro . Methods Primary hepatic stellate cells were isolated and cultured from Sprague-Dawley rats. After activating and inducing primary hepatic stellate cells from qHSC to aHSC，the activated hepatic stellate cells model in vitro was established. Then we observed the morphological changes of static hepatic stellate cells and activated hepatic stellate cells with inverted phase contrast microscope. Cultured hepatic stellate cells were treated with different concentrations of loureirin A and the inhibitory rate of HSCs proliferation was measured by MTT assay. The expression of Frizzled-4 was measured by western blot analysis. The content of α-SMA and TGF-β1 in the cultured HSCs'supernatant were measured by enzyme-linked immunosorbent assay（ELISA） . Results Loureirin A the proliferation of inhibited activated hepatic stellate cells in a time-dose-dependent manner compared with the control group，IC50=0.30 μg/μL. After loureirinA treatment of the HSCs，western blot analysis showed that Frizzled-4 expression level was obviously lower than control group. Loureirin A also inhibited α-SMA and TGFβ1（P＜0.05）secretion in the cultured HSCs'supernatant in different degree by the assay of ELISA. Conclusions The molecular mechanism of Loureirin A and Wnt signaling pathway mediated anti-hepatic fibrosis and anti-angiogenesis may involve down-regulation the expression of Frizzled-4，inhibiting the synthesis and secretion of α-SMA，TGF-β1and the proliferation of HSCs.
［Abstract］Objective To investigate the molecular mechanism of Loureirin A mediated anti-hepatic fibrosis by evaluting its effects on proliferation ，secretion of α-smooth muscle actin（α-SMA）and transforming growth factor-beta1（TGF-β1），and expression of rat hepatic stellate cells in vitro . Methods Primary hepatic stellate cells were isolated and cultured from Sprague-Dawley rats. After activating and inducing primary hepatic stellate cells from qHSC to aHSC，the activated hepatic stellate cells model in vitro was established. Then we observed the morphological changes of static hepatic stellate cells and activated hepatic stellate cells with inverted phase contrast microscope. Cultured hepatic stellate cells were treated with different concentrations of loureirin A and the inhibitory rate of HSCs proliferation was measured by MTT assay. The expression of Frizzled-4 was measured by western blot analysis. The content of α-SMA and TGF-β1 in the cultured HSCs'supernatant were measured by enzyme-linked immunosorbent assay（ELISA） . Results Loureirin A the proliferation of inhibited activated hepatic stellate cells in a time-dose-dependent manner compared with the control group，IC50=0.30 μg/μL. After loureirinA treatment of the HSCs，western blot analysis showed that Frizzled-4 expression level was obviously lower than control group. Loureirin A also inhibited α-SMA and TGFβ1（P＜0.05）secretion in the cultured HSCs'supernatant in different degree by the assay of ELISA. Conclusions The molecular mechanism of Loureirin A and Wnt signaling pathway mediated anti-hepatic fibrosis and anti-angiogenesis may involve down-regulation the expression of Frizzled-4，inhibiting the synthesis and secretion of α-SMA，TGF-β1and the proliferation of HSCs.
2016, 37(06)
Abstract:
［Abstract］Objective To establish the resist-fatigue animal test through using Maca， in order to provide reference for the relevant departments to exploit new sports nutrition . Methods Thirty male KM mice were randomly divided into Quiet control group（A），Exhaustive group（B，high altitude），Maca exhaustive group（C）， there were 10 mice in each group. Drinking water containing Maca（Maca =500 mg/L）was supplied in group C. Additional exhaustive group（D，low altitude）was set．Thirty days later， all mice were killed after one exhaustive test， then we tested the exhaustive time，the level of T-SOD and liver glycogen. Results The exhaustive time of group C was significantly longer than group B（P＜0.05）， the T-SOD level of group C was significantly higher than group A and group B（P＜0.05）， and the liver glycogen content of group C was significantly higher than group B（P＜0.05）. Conclusion Maca can raise T-SOD and liver glycogen level of the mice after exhaustive swimming， and has function of resist-fatigue.
［Abstract］Objective To establish the resist-fatigue animal test through using Maca， in order to provide reference for the relevant departments to exploit new sports nutrition . Methods Thirty male KM mice were randomly divided into Quiet control group（A），Exhaustive group（B，high altitude），Maca exhaustive group（C）， there were 10 mice in each group. Drinking water containing Maca（Maca =500 mg/L）was supplied in group C. Additional exhaustive group（D，low altitude）was set．Thirty days later， all mice were killed after one exhaustive test， then we tested the exhaustive time，the level of T-SOD and liver glycogen. Results The exhaustive time of group C was significantly longer than group B（P＜0.05）， the T-SOD level of group C was significantly higher than group A and group B（P＜0.05）， and the liver glycogen content of group C was significantly higher than group B（P＜0.05）. Conclusion Maca can raise T-SOD and liver glycogen level of the mice after exhaustive swimming， and has function of resist-fatigue.
2016, 37(06)
Abstract:
［Abstract］Objective To construct a large animal model of ischemic heart disease induced by acute myocardial infarction （AMI）. Methods Male Diannan Mini-pigs were selected （n=8）， weight 20 ± 4 Kg. After general anesthesia and endotracheal intubation， blood pressure and electrocardiograph were monitored. Parasternal incision was taken and thoracotomyv was performed at 4th-to-5th intercostalgap. Pericardium was opened and left anterior descending artery was ligated. Echocardiography was performed at baseline，postoperative 28 and 60 day. Euthanasia and heart explanted was conducted at 60 day of experimental pig. After gross examination of the heart，the histological examination was used to assess myocardial infarction by using H&E staining. Results All of the pigs were presented with ventricular arrhythmias in varying degrees after ligation of the left anterior descending artery， of whom 2 died of refractory ventricular fibrillation，the mortality was 25%. Echocardiography assessment showed the loss left ventricular function， the thinning of left ventricular apical and the enlargement of left ventricule. Histological examination revealed that myocardial fibrosis and fibrous scar were formed in myocardial infarction area. Conclusion The ligation of left anterior descending artery through thoracotomy under direct vision is a reliable strategy for construction of AMI porcine model and the experimental porcine can develop ischemic cardiac dysfunction progressively.
［Abstract］Objective To construct a large animal model of ischemic heart disease induced by acute myocardial infarction （AMI）. Methods Male Diannan Mini-pigs were selected （n=8）， weight 20 ± 4 Kg. After general anesthesia and endotracheal intubation， blood pressure and electrocardiograph were monitored. Parasternal incision was taken and thoracotomyv was performed at 4th-to-5th intercostalgap. Pericardium was opened and left anterior descending artery was ligated. Echocardiography was performed at baseline，postoperative 28 and 60 day. Euthanasia and heart explanted was conducted at 60 day of experimental pig. After gross examination of the heart，the histological examination was used to assess myocardial infarction by using H&E staining. Results All of the pigs were presented with ventricular arrhythmias in varying degrees after ligation of the left anterior descending artery， of whom 2 died of refractory ventricular fibrillation，the mortality was 25%. Echocardiography assessment showed the loss left ventricular function， the thinning of left ventricular apical and the enlargement of left ventricule. Histological examination revealed that myocardial fibrosis and fibrous scar were formed in myocardial infarction area. Conclusion The ligation of left anterior descending artery through thoracotomy under direct vision is a reliable strategy for construction of AMI porcine model and the experimental porcine can develop ischemic cardiac dysfunction progressively.
2016, 37(06)
Abstract:
［Abstract］Objective To investigate the correlation between ADAM33 T1， S2 gene polymorphism and Bronchial asthma risk in china. Methods We retrived the relevant published studies about ADAM33 T1，S2 gene polymorphism and bronchial asthma risk. Then we divided the population into Chinese and other Asian population. Odds ratio（OR）of Case group and control group was selected as the effect index. Stata 11.0 software was used to calculate heterogeneity test，ORs and 95%CI of two areas，and gave the forest plot and funnel plot of meta results. Results A total of 27 studies were included in this analysis，18 studies in ADAM33 T1 site were 3881 cases in case group， and 3780 cases in control group; and 14 studies in ADAM33 S2 site were 3222 cases in case group，and 3513 cases in control group. Additive model，dominant model，recessive model of ADAM33 T1 in Chinese had association with the susceptibility of bronchial asthma. The results were OR=1.488，95% CI：1.002-2.167 in Additive model， OR=1.619，95%CI：1.059-2.475 in dominant model；OR=2.523，95%CI：1.910-3.333 in recessive model. Three models of ADAM33 T1 in other Asian country had no association with the susceptibility of Bronchial Asthma. Three gene model of ADAM33 S2 in Asian had no association with bronchial asthma susceptibility. Except ADAM33 T1 polymorphism in recessive model， other mode of T1， S2 had no publication bias in Chinese population. Conclusion There are association between ADAM33 T1 gene polymorphism and bronchial asthma， but ADAM33 S2 gene polymorphism and bronchial asthma have no association in Chinese population.
［Abstract］Objective To investigate the correlation between ADAM33 T1， S2 gene polymorphism and Bronchial asthma risk in china. Methods We retrived the relevant published studies about ADAM33 T1，S2 gene polymorphism and bronchial asthma risk. Then we divided the population into Chinese and other Asian population. Odds ratio（OR）of Case group and control group was selected as the effect index. Stata 11.0 software was used to calculate heterogeneity test，ORs and 95%CI of two areas，and gave the forest plot and funnel plot of meta results. Results A total of 27 studies were included in this analysis，18 studies in ADAM33 T1 site were 3881 cases in case group， and 3780 cases in control group; and 14 studies in ADAM33 S2 site were 3222 cases in case group，and 3513 cases in control group. Additive model，dominant model，recessive model of ADAM33 T1 in Chinese had association with the susceptibility of bronchial asthma. The results were OR=1.488，95% CI：1.002-2.167 in Additive model， OR=1.619，95%CI：1.059-2.475 in dominant model；OR=2.523，95%CI：1.910-3.333 in recessive model. Three models of ADAM33 T1 in other Asian country had no association with the susceptibility of Bronchial Asthma. Three gene model of ADAM33 S2 in Asian had no association with bronchial asthma susceptibility. Except ADAM33 T1 polymorphism in recessive model， other mode of T1， S2 had no publication bias in Chinese population. Conclusion There are association between ADAM33 T1 gene polymorphism and bronchial asthma， but ADAM33 S2 gene polymorphism and bronchial asthma have no association in Chinese population.
Difference of Metallothionein Gene G-201A Polymorphism in Type 2 Diabetes and Essential Hypertension
2016, 37(06)
Abstract:
［Abstract］Objective To investigate the difference of MT4 G-201A of Metallothionein （metallotionein， MT） family gene locus single nucleotide polymorphisms （SNPs） in type 2 diabetes and essential hypertension. Mothods We selected 324 cases of patients with type 2 diabetes， 301 cases of patients with essential hypertension， 301 case of normal physical examination population. Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）analysis technology was used to detect MT4 G-201A polymorphism. Results （1） The genotype GG， GA and AA frequency of MT4 G-201A in 324 cases of patients with type 2 diabetes，301 cases of patients with essential hypertension，301 case of normal physical examination population were 39.5%，46.6%，13.9%；42.2%，48.5%，9.3%；and 42.5%，51.5%，6.0%， respectively. The genotypes in all the distribution were in Hardy-Weinberg equilibrium （P＞0.05）.（2） MT4 G-201A polymorphism: Three groups of genotype and allele frequency distribution （G and A） had differences （P＜0.05）; there was no difference between essential hypertension group and normal group （P＞0.05）；there was a difference between type 2 diabetes group and the normal group（P＜0.05）；there was no significant difference in genotype and allele frequency distribution（P＞0.05）.（3）MT4 G-201A polymorphism in Men: Three groups of genotype and allele frequency distribution had differences （P＜0.05）. In essential hypertension and type 2 diabetes group，there was no significant difference in genotype and allele frequency distribution（P＞0.05）.MT4 G-201A polymorphism between women:Three groups of genotype and allele frequency distribution had differences （P＜0.05），there was a difference between type 2 diabetes and normal group genotypes （P＜0.05）. Conclusions （1）There is correlation of MT gene G-210A polymorphism and type 2 diabetes， no association with essential hypertension. （2） There is a difference of MT gene G-210A polymorphism in type 2 diabetes in women， no difference in men.
［Abstract］Objective To investigate the difference of MT4 G-201A of Metallothionein （metallotionein， MT） family gene locus single nucleotide polymorphisms （SNPs） in type 2 diabetes and essential hypertension. Mothods We selected 324 cases of patients with type 2 diabetes， 301 cases of patients with essential hypertension， 301 case of normal physical examination population. Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）analysis technology was used to detect MT4 G-201A polymorphism. Results （1） The genotype GG， GA and AA frequency of MT4 G-201A in 324 cases of patients with type 2 diabetes，301 cases of patients with essential hypertension，301 case of normal physical examination population were 39.5%，46.6%，13.9%；42.2%，48.5%，9.3%；and 42.5%，51.5%，6.0%， respectively. The genotypes in all the distribution were in Hardy-Weinberg equilibrium （P＞0.05）.（2） MT4 G-201A polymorphism: Three groups of genotype and allele frequency distribution （G and A） had differences （P＜0.05）; there was no difference between essential hypertension group and normal group （P＞0.05）；there was a difference between type 2 diabetes group and the normal group（P＜0.05）；there was no significant difference in genotype and allele frequency distribution（P＞0.05）.（3）MT4 G-201A polymorphism in Men: Three groups of genotype and allele frequency distribution had differences （P＜0.05）. In essential hypertension and type 2 diabetes group，there was no significant difference in genotype and allele frequency distribution（P＞0.05）.MT4 G-201A polymorphism between women:Three groups of genotype and allele frequency distribution had differences （P＜0.05），there was a difference between type 2 diabetes and normal group genotypes （P＜0.05）. Conclusions （1）There is correlation of MT gene G-210A polymorphism and type 2 diabetes， no association with essential hypertension. （2） There is a difference of MT gene G-210A polymorphism in type 2 diabetes in women， no difference in men.
2016, 37(06)
Abstract:
［Abstract］Objectives To understand the social support levels among breast cancer patients in Yunnan， as well as to explore the factors associated with social support. Methods According to the unified inclusion and exclusion criteria，121 breast cancer in-patients with chemotherapy were interviewed with structured questionnaire. Social demographic characteristics， Xiao's Social Support Rating Scale，General Self-Efficacy Scale，clinical and experimental data were collected. SPSS version19.0 was used to analyze the frequency and the correlation between social support and influential variables were analyzed by using the chi-square test and non-parametric test. Results The levels of social support in total， objective social support， subjective social support and utilization degree for breast cancer patients were（49.43±5.69），（13.35±2.51），（27.59±3.78），（8.50±1.98）respectively. Marriage status and self-efficacy were associated with social support level significantly. The influencing factors such as age， education level， marital status， occupation， income， place of residence， religion， medical expenses payment type， self-efficacy were included in the univariate analysis. However， only marital status and self efficacy were positively correlated with social support（p<0.05）. Conclusions The breast cancer patients in Yunnan have a higher social support level overall. Having-marriage status and higher self-efficacy have a positive influence on breast cancer in patients' social support level.
［Abstract］Objectives To understand the social support levels among breast cancer patients in Yunnan， as well as to explore the factors associated with social support. Methods According to the unified inclusion and exclusion criteria，121 breast cancer in-patients with chemotherapy were interviewed with structured questionnaire. Social demographic characteristics， Xiao's Social Support Rating Scale，General Self-Efficacy Scale，clinical and experimental data were collected. SPSS version19.0 was used to analyze the frequency and the correlation between social support and influential variables were analyzed by using the chi-square test and non-parametric test. Results The levels of social support in total， objective social support， subjective social support and utilization degree for breast cancer patients were（49.43±5.69），（13.35±2.51），（27.59±3.78），（8.50±1.98）respectively. Marriage status and self-efficacy were associated with social support level significantly. The influencing factors such as age， education level， marital status， occupation， income， place of residence， religion， medical expenses payment type， self-efficacy were included in the univariate analysis. However， only marital status and self efficacy were positively correlated with social support（p<0.05）. Conclusions The breast cancer patients in Yunnan have a higher social support level overall. Having-marriage status and higher self-efficacy have a positive influence on breast cancer in patients' social support level.
2016, 37(06)
Abstract:
［Abstract］Objective To analyze the situation of health-risk behaviors of the students in specialty medical science college of Yunnan and its relationship with negative life events， and provide scientific evidence for lowering the occurrence rate of health-risk behaviors. Methods The stratified randomised cluster-sampling method was used to select 576 students of specialty medical science college， and the occurrence rate of health-risk behaviors and life events conditions were estimated by Youth Health-Risk Behavior Questionnaire and Adolescents Self-Rating Life Events Checklist. Descriptive statistic analysis， Chi-square test and Logistic regression analysis was used to analyze the data. Results Among the students of specialty medical science college in Yunnan， the rate of smoking was 3.1%，the rate of drinking was 4.9%，the rate of food refusal was 39.6%， the rate of fighting was 13.5%，the rate of obsessed with the internet was 26.4%，and the rate of substance abuse was 3.1%. There were significant differences between the students of different genders（P＜0.05） or specialities（P＜0.05）. Logistic regression analysis showed total score of negative life events had positive influence on drinking （OR=1.017，P＜0.05）， food refusal （OR＝1.018，P＜0.05）， fighting （OR＝1.021，P＜0.05）， obsessed with the internet（OR＝1.025，P＜0.05）， substance abuse（OR＝1.025，P＜0.05）. The factor of interpersonal relationship had positive influence on drinking（OR＝1.162，P＜0.05）， food refusal（OR＝1.102，P＜0.05）， substance abuse（OR＝1.260，P＜0.05）. The factor of punished had positive influence on fighting（OR＝1.144，P＜0.05）， and had negative influence on drinking（OR＝0.891，P＜0.05）. The factor of study pressure had positive influence on fighting（OR＝1.143，P＜0.05）， and the factor of loss had negative influence on fighting（OR＝1.144，P＜0.05）. Conclusion Negative life events are influencing factors for health-risk behaviors of the students in specialty medical science college of Yunnan， and there is difference on the way that the factor of life events influencing health-risk behaviors.
［Abstract］Objective To analyze the situation of health-risk behaviors of the students in specialty medical science college of Yunnan and its relationship with negative life events， and provide scientific evidence for lowering the occurrence rate of health-risk behaviors. Methods The stratified randomised cluster-sampling method was used to select 576 students of specialty medical science college， and the occurrence rate of health-risk behaviors and life events conditions were estimated by Youth Health-Risk Behavior Questionnaire and Adolescents Self-Rating Life Events Checklist. Descriptive statistic analysis， Chi-square test and Logistic regression analysis was used to analyze the data. Results Among the students of specialty medical science college in Yunnan， the rate of smoking was 3.1%，the rate of drinking was 4.9%，the rate of food refusal was 39.6%， the rate of fighting was 13.5%，the rate of obsessed with the internet was 26.4%，and the rate of substance abuse was 3.1%. There were significant differences between the students of different genders（P＜0.05） or specialities（P＜0.05）. Logistic regression analysis showed total score of negative life events had positive influence on drinking （OR=1.017，P＜0.05）， food refusal （OR＝1.018，P＜0.05）， fighting （OR＝1.021，P＜0.05）， obsessed with the internet（OR＝1.025，P＜0.05）， substance abuse（OR＝1.025，P＜0.05）. The factor of interpersonal relationship had positive influence on drinking（OR＝1.162，P＜0.05）， food refusal（OR＝1.102，P＜0.05）， substance abuse（OR＝1.260，P＜0.05）. The factor of punished had positive influence on fighting（OR＝1.144，P＜0.05）， and had negative influence on drinking（OR＝0.891，P＜0.05）. The factor of study pressure had positive influence on fighting（OR＝1.143，P＜0.05）， and the factor of loss had negative influence on fighting（OR＝1.144，P＜0.05）. Conclusion Negative life events are influencing factors for health-risk behaviors of the students in specialty medical science college of Yunnan， and there is difference on the way that the factor of life events influencing health-risk behaviors.
2016, 37(06)
Abstract:
［Abstract］Objective To evaluate the correlation of the single nucleotide polymorphisms （SNPs） in CDH13 with non-small cell lung cancer （NSCLC）. Methods 115 patients with NSCLC and 110 healthy controls were included in present study. Two SNPs （rs11646213 and rs7195409） in CDH13 were genotyped using TaqMan method. The association of these two SNPs with NSCLC was calculated and assessed. Results The genotypic and allelic frequencies of rs11646213 showed significant difference between NSCLC patients and the control group （P＜0.05），（OR＝0.464，95% CI：0.273～0.789）. The genotypic and allelic frequencies of rs7195409 showed significant difference between the stage I+II and stage III+IV groups （P＜0.05），（OR＝0.491，95% CI：0.243～0.991）. Conclusions The rs16146213 has a strong association with NSCLC and G allelic showed a protective effect. The rs7195409 has a strong association between stage I+II and III + IV in NSCLC， and G allele may play a protective role in the development of NSCLC.
［Abstract］Objective To evaluate the correlation of the single nucleotide polymorphisms （SNPs） in CDH13 with non-small cell lung cancer （NSCLC）. Methods 115 patients with NSCLC and 110 healthy controls were included in present study. Two SNPs （rs11646213 and rs7195409） in CDH13 were genotyped using TaqMan method. The association of these two SNPs with NSCLC was calculated and assessed. Results The genotypic and allelic frequencies of rs11646213 showed significant difference between NSCLC patients and the control group （P＜0.05），（OR＝0.464，95% CI：0.273～0.789）. The genotypic and allelic frequencies of rs7195409 showed significant difference between the stage I+II and stage III+IV groups （P＜0.05），（OR＝0.491，95% CI：0.243～0.991）. Conclusions The rs16146213 has a strong association with NSCLC and G allelic showed a protective effect. The rs7195409 has a strong association between stage I+II and III + IV in NSCLC， and G allele may play a protective role in the development of NSCLC.
2016, 37(06)
Abstract:
［Abstract］Objective To compare the three kinds of methods for in vitro primary culturing of rheumatoid arthritis synovial fibroblast-like cells（RASFs）， in order to get fast and effective culture methods. Methods Synovial tissue from RA synovial arthroscopic resection were treated by collagenase digestion method， modified tissue culture method，double enzyme digestion method respectively. By using an inverted phase contrast microscope， cell morphology and growth characteristics were observed and identified with vimentin staining. Trypan blue was used to count the number of living cells after culturing for 14d. Results The three primary methods could successfully isolate and culture RASFs，and RASFs met the morphological characteristics of vimentin-positive cells＞95%，namely，the proportion of RASFs cell confluence was 70% after 16-20days by the collagenase digestion method，whose cell confluence proportion reached 95% after 4 weeks；and the cell confluence proportion was above 70% after 10-14days by modified tissue culture method，and the cell confluence proportion reached 85% after 4 weeks by the double enzyme digestion method. The comparison of the viable cells number cultured same number of synovial tissue by the three methods show the viable cells number cultured by the modified tissue culture method were（1.60±0.08）×106， those by the collagenase digestion method were （1.41±0.08）×106， those by the double enzyme digestion method were（1.19±0.05）×106， which were with significant difference among them（P＜0.05）.The comparison of incubation time of RASFs primary cells showed it took（267.50±16.58）mins by the collagenase digestion method，（183.75±11.08）mins by the double enzyme digestion method， and 149.10±13.71mins by the modified tissue culture method，with significant differences（P＜0.05）．Conclusion Modified tissue culture for RASFs is an efficient and fast culture method， the number and purity of RASFs can meet the requirements for biology experiments.
［Abstract］Objective To compare the three kinds of methods for in vitro primary culturing of rheumatoid arthritis synovial fibroblast-like cells（RASFs）， in order to get fast and effective culture methods. Methods Synovial tissue from RA synovial arthroscopic resection were treated by collagenase digestion method， modified tissue culture method，double enzyme digestion method respectively. By using an inverted phase contrast microscope， cell morphology and growth characteristics were observed and identified with vimentin staining. Trypan blue was used to count the number of living cells after culturing for 14d. Results The three primary methods could successfully isolate and culture RASFs，and RASFs met the morphological characteristics of vimentin-positive cells＞95%，namely，the proportion of RASFs cell confluence was 70% after 16-20days by the collagenase digestion method，whose cell confluence proportion reached 95% after 4 weeks；and the cell confluence proportion was above 70% after 10-14days by modified tissue culture method，and the cell confluence proportion reached 85% after 4 weeks by the double enzyme digestion method. The comparison of the viable cells number cultured same number of synovial tissue by the three methods show the viable cells number cultured by the modified tissue culture method were（1.60±0.08）×106， those by the collagenase digestion method were （1.41±0.08）×106， those by the double enzyme digestion method were（1.19±0.05）×106， which were with significant difference among them（P＜0.05）.The comparison of incubation time of RASFs primary cells showed it took（267.50±16.58）mins by the collagenase digestion method，（183.75±11.08）mins by the double enzyme digestion method， and 149.10±13.71mins by the modified tissue culture method，with significant differences（P＜0.05）．Conclusion Modified tissue culture for RASFs is an efficient and fast culture method， the number and purity of RASFs can meet the requirements for biology experiments.
2016, 37(06)
Abstract:
［Abstract］Objective To investigate the roles of percutaneous coronary intervention in improving left ventricular function，reducing incidence of cardiovascular events of patients with ischemic cardiomyopathy. Methods Ninety cases of ischemic cardiomyopathy patients from August 2012 to August 2015 were randomly selected， these patients were divided into two groups， the study group（n = 45） and control group （n = 45） according to the random number table. The control group of patients were given routine treatment， while the study group of patients were given conventional treatment combined with percutaneous coronary intervention. Results The LVEDV， LVEF of the study group were significantly higher （P＜0.05）， the incidence of acute myocardial infarction onset， hospitalization rates again， mortality rate 8.89% （4/45）， 13.33% （6/45）， 2.22 % （1/45） were significantly lower than the control group 24.44% （11/45）， 40.00% （18/45）， 17.78% （8/45） （P＜0.05）. Conclusion Percutaneous coronary intervention can improve left ventricular function and reduce the incidence of cardiovascular events of patients with ischemic cardiomyopathy.
［Abstract］Objective To investigate the roles of percutaneous coronary intervention in improving left ventricular function，reducing incidence of cardiovascular events of patients with ischemic cardiomyopathy. Methods Ninety cases of ischemic cardiomyopathy patients from August 2012 to August 2015 were randomly selected， these patients were divided into two groups， the study group（n = 45） and control group （n = 45） according to the random number table. The control group of patients were given routine treatment， while the study group of patients were given conventional treatment combined with percutaneous coronary intervention. Results The LVEDV， LVEF of the study group were significantly higher （P＜0.05）， the incidence of acute myocardial infarction onset， hospitalization rates again， mortality rate 8.89% （4/45）， 13.33% （6/45）， 2.22 % （1/45） were significantly lower than the control group 24.44% （11/45）， 40.00% （18/45）， 17.78% （8/45） （P＜0.05）. Conclusion Percutaneous coronary intervention can improve left ventricular function and reduce the incidence of cardiovascular events of patients with ischemic cardiomyopathy.
2016, 37(06)
Abstract:
［Abstract］Objective To investigate the clinical effect of ilium grafting combined with Duhuojishengtang on the delay fracture healing and explore the effect on hemorheology. Methods Eighty patients with delayed fracture healing were randomly divided into two groups. Forty patients in control group were treated by ilium grafting and 40 patients in observation group were treated by ilium grafting combined with Duhuojishengtang. The clinical effect was observed after treatment and indexes of hemorheology were recorded after different treatment time. Results The cure rate of observation group was 62.5%， which was significantly higher than that of control group 35.0%， the effective rate of observation group was 92.5%， which was significantly higher than that of control group 72.5% （P＜0.01）. There were no significant differences between two groups in indexes of hemorheology after ilium grafting （P＞0.05）. After the first， second， third course， the indexes of hemorheology of observation group were better than those of control group， with statistically significant differences （P＜0.05）. Conclusions Ilium grafting combined with duhuojishengtang can improve the indexes of hemorheology， improve the cure and effective rate and also has high security. It has high clinical value and worth of popularization and application.
［Abstract］Objective To investigate the clinical effect of ilium grafting combined with Duhuojishengtang on the delay fracture healing and explore the effect on hemorheology. Methods Eighty patients with delayed fracture healing were randomly divided into two groups. Forty patients in control group were treated by ilium grafting and 40 patients in observation group were treated by ilium grafting combined with Duhuojishengtang. The clinical effect was observed after treatment and indexes of hemorheology were recorded after different treatment time. Results The cure rate of observation group was 62.5%， which was significantly higher than that of control group 35.0%， the effective rate of observation group was 92.5%， which was significantly higher than that of control group 72.5% （P＜0.01）. There were no significant differences between two groups in indexes of hemorheology after ilium grafting （P＞0.05）. After the first， second， third course， the indexes of hemorheology of observation group were better than those of control group， with statistically significant differences （P＜0.05）. Conclusions Ilium grafting combined with duhuojishengtang can improve the indexes of hemorheology， improve the cure and effective rate and also has high security. It has high clinical value and worth of popularization and application.
2016, 37(06)
Abstract:
［Abstract］Objective To explore the influence of different methods of bandaging on the postoperative intracranial pressure of patients with severe brain injury patients after decompression craniectomy. Methods The standard decompressive craniectomy was use for the 36 cases of severe traumatic brain injury patients， and the intracranial pressure monitoring sensor probe was indwelled in operaion. Two different dressing methods of elasticity mesh cap and applicator were used for the patients respectively at 0h， 72h， 120h and 168h after operation， and the value of intracranial pressure was monitored and recorded. Result The intracranial pressure of elastic cap were significantly higher than the applicator respectively in operation immediate postoperative 72h， 120h and 168h（P ＜0.05）， the difference was statistically significant. Conclusions The intracranial pressure of elastic cap is significantly higher than the applicator at different times after the surgery group.
［Abstract］Objective To explore the influence of different methods of bandaging on the postoperative intracranial pressure of patients with severe brain injury patients after decompression craniectomy. Methods The standard decompressive craniectomy was use for the 36 cases of severe traumatic brain injury patients， and the intracranial pressure monitoring sensor probe was indwelled in operaion. Two different dressing methods of elasticity mesh cap and applicator were used for the patients respectively at 0h， 72h， 120h and 168h after operation， and the value of intracranial pressure was monitored and recorded. Result The intracranial pressure of elastic cap were significantly higher than the applicator respectively in operation immediate postoperative 72h， 120h and 168h（P ＜0.05）， the difference was statistically significant. Conclusions The intracranial pressure of elastic cap is significantly higher than the applicator at different times after the surgery group.
2016, 37(06)
Abstract:
［Abstract］Objective To study the distribution and antimicrobial susceptibilities of anaerobic bacteria from periodontal pockets of patients with chronic periodontitis， so as to guide the anti-infection therapy. Methods Eighty-four patients with chronic periodontitis were enrolled in this study. The anaerobes were isolated from periodontal pockets of patients with chronic periodontitis. Antimicrobial susceptibility of the anaerobes to seven kinds of antibiotics （like metronidazole， penicillin， cefmetazole， etc.） was tested with the agar dilution technique. Results A total of 305 cultivable anaerobes were isolated from periodontal pockets of 84 patients with chronic periodontitis. All patients were infected by anaerobes. 59.34% of cultivable anaerobes were gram-positive anaerobes，and 40.66% were gram-negative anaerobes. The P.melanin ogenica and P.gingivalis were the most common among the cultivable anaerobes， accounting for 51.19% and 55.95%， followed by P.intermedia （36.90%）， P.micros（35.71%），F.mucleatum （33.33%）， Capnocytophaga ssp（27.38%），Actinomyces ssp （10.71%）， and so on. Most isolated anaerobes were sensitive to seven antibiotics. All isolated anaerobes were susceptible to Chloromycetin and Imipenem. 98.03% of isolated anaerobes were susceptible to Amoxicillin. Approximately 76% to 88% of isolated anaerobes were susceptible to Cefmetazole and Metronidazole. Susceptibility to Clindamycinum and Penicillin were 65.91% and 55.73%. Conclusions There is a close relationship between chronic periodontitis and anaerobes. P.melanin ogenica and P.gingivalis are the most frequently isolated anaerobes from periodontal pockets of patients with chronic periodontitis. Chloromycetin and Imipenem can be used for the treatment of anaerobes infected chronic periodontitis.
［Abstract］Objective To study the distribution and antimicrobial susceptibilities of anaerobic bacteria from periodontal pockets of patients with chronic periodontitis， so as to guide the anti-infection therapy. Methods Eighty-four patients with chronic periodontitis were enrolled in this study. The anaerobes were isolated from periodontal pockets of patients with chronic periodontitis. Antimicrobial susceptibility of the anaerobes to seven kinds of antibiotics （like metronidazole， penicillin， cefmetazole， etc.） was tested with the agar dilution technique. Results A total of 305 cultivable anaerobes were isolated from periodontal pockets of 84 patients with chronic periodontitis. All patients were infected by anaerobes. 59.34% of cultivable anaerobes were gram-positive anaerobes，and 40.66% were gram-negative anaerobes. The P.melanin ogenica and P.gingivalis were the most common among the cultivable anaerobes， accounting for 51.19% and 55.95%， followed by P.intermedia （36.90%）， P.micros（35.71%），F.mucleatum （33.33%）， Capnocytophaga ssp（27.38%），Actinomyces ssp （10.71%）， and so on. Most isolated anaerobes were sensitive to seven antibiotics. All isolated anaerobes were susceptible to Chloromycetin and Imipenem. 98.03% of isolated anaerobes were susceptible to Amoxicillin. Approximately 76% to 88% of isolated anaerobes were susceptible to Cefmetazole and Metronidazole. Susceptibility to Clindamycinum and Penicillin were 65.91% and 55.73%. Conclusions There is a close relationship between chronic periodontitis and anaerobes. P.melanin ogenica and P.gingivalis are the most frequently isolated anaerobes from periodontal pockets of patients with chronic periodontitis. Chloromycetin and Imipenem can be used for the treatment of anaerobes infected chronic periodontitis.
2016, 37(06)
Abstract:
［Abstract］Objective To explore the short-term and long-term clinical effect of leep knife treatment of chronic cervicitis. Methods A single-center， prospective， randomized， controlled study was used， 120 patients with chronic cervicitis were randomly divided into observation group and control group， each group of 60 patients. Patients in the observation group were treated with （LEEP） knife， parameters setting frequency was 3.8 MHz， power was 30 ~ 40W， patients in the control group were treated with microwave. The intraoperative and postoperative recovery and relapse were compared between two groups. Results The intraoperative blood loss， operative time， postoperative vaginal discharge time in the observation group were significantly less than control group， there was a significantly statistical difference （P < 0.001）. After two weeks and three months ， the cure rate in observation group were 70% and 98.33% respectively， the cure rate in control group were 16.67% and 51.67%， there was a significantly statistical difference （P < 0.01）. After 2 weeks and 3 months， the infection rate and recurrence rate in the observation group were significantly lower than the control group， there was a significantly statistical difference （P < 0.05）. Conclusion Leep knife for treatment of chronic cervicitis patients has lower postoperative recurrence， lower infection rate and higher cure rate in short-term and long-term， and less intraoperative trauma， shorter operation time， and has important clinical significance for the prognosis of patients.
［Abstract］Objective To explore the short-term and long-term clinical effect of leep knife treatment of chronic cervicitis. Methods A single-center， prospective， randomized， controlled study was used， 120 patients with chronic cervicitis were randomly divided into observation group and control group， each group of 60 patients. Patients in the observation group were treated with （LEEP） knife， parameters setting frequency was 3.8 MHz， power was 30 ~ 40W， patients in the control group were treated with microwave. The intraoperative and postoperative recovery and relapse were compared between two groups. Results The intraoperative blood loss， operative time， postoperative vaginal discharge time in the observation group were significantly less than control group， there was a significantly statistical difference （P < 0.001）. After two weeks and three months ， the cure rate in observation group were 70% and 98.33% respectively， the cure rate in control group were 16.67% and 51.67%， there was a significantly statistical difference （P < 0.01）. After 2 weeks and 3 months， the infection rate and recurrence rate in the observation group were significantly lower than the control group， there was a significantly statistical difference （P < 0.05）. Conclusion Leep knife for treatment of chronic cervicitis patients has lower postoperative recurrence， lower infection rate and higher cure rate in short-term and long-term， and less intraoperative trauma， shorter operation time， and has important clinical significance for the prognosis of patients.
2016, 37(06)
Abstract:
［Abstract］Objective To explore the efficacy and safety of large and conventional fractionated three dimensional conformal radiation therapy for non small cell lung cancer with superior vena cava compression syndrome，and to provide clinical reference for improving the prognosis of patients with non small cell lung cancer. Methods The general data of 63 patients with non small cell lung cancer complicated with superior vena cava syndrome treated in Lu'an Hospital of Traditional Chinese Medicine from January 2010 to December 2012 were analyzed retrospectively. According to the different treatment methods， the patients were divided into observation group （n＝28）and control group（n＝35）.The control group was given conventional fractionated three-dimensional conformal radiation therapy，three dimensional conformal radiation therapy was given to the observation group. The clinical efficacy， toxicity， and 1，2 years survival rate of the two groups were compared. Results The complete remission rate of observation group was 96.43%， which was significantly higher than 74.29% in the control group（P＜0.05）. The complete remission in the observation group was 89.29% in two weeks， which was significantly higher than 62.86% in the control group （P＜0.05）. The total effective rate of observation group was 85.71%， which was significantly higher than that 62.86%in the control group （P＜0.05）. The clinical benefit rate of observation group was 96.43%， and there was no significant difference between the two groups （P＞0.05）. There was no significant difference in the incidence of adverse reactions between the two groups （P＞0.05）. There was no significant difference in 1，2 year survival rate between the two groups （P＞0.05）. Conclusion Three dimensional conformal radiation therapy for non small cell lung cancer patients with superior vena cava syndrome is superior to conventional treatment， and does not increase the toxic side effects， which is worthy of clinical attention.
［Abstract］Objective To explore the efficacy and safety of large and conventional fractionated three dimensional conformal radiation therapy for non small cell lung cancer with superior vena cava compression syndrome，and to provide clinical reference for improving the prognosis of patients with non small cell lung cancer. Methods The general data of 63 patients with non small cell lung cancer complicated with superior vena cava syndrome treated in Lu'an Hospital of Traditional Chinese Medicine from January 2010 to December 2012 were analyzed retrospectively. According to the different treatment methods， the patients were divided into observation group （n＝28）and control group（n＝35）.The control group was given conventional fractionated three-dimensional conformal radiation therapy，three dimensional conformal radiation therapy was given to the observation group. The clinical efficacy， toxicity， and 1，2 years survival rate of the two groups were compared. Results The complete remission rate of observation group was 96.43%， which was significantly higher than 74.29% in the control group（P＜0.05）. The complete remission in the observation group was 89.29% in two weeks， which was significantly higher than 62.86% in the control group （P＜0.05）. The total effective rate of observation group was 85.71%， which was significantly higher than that 62.86%in the control group （P＜0.05）. The clinical benefit rate of observation group was 96.43%， and there was no significant difference between the two groups （P＞0.05）. There was no significant difference in the incidence of adverse reactions between the two groups （P＞0.05）. There was no significant difference in 1，2 year survival rate between the two groups （P＞0.05）. Conclusion Three dimensional conformal radiation therapy for non small cell lung cancer patients with superior vena cava syndrome is superior to conventional treatment， and does not increase the toxic side effects， which is worthy of clinical attention.
2016, 37(06)
Abstract:
［Abstract］Objective To study clinical effect of laparoscopy combined with gestrinone in treatment of ovarian endometriosis cyst and the prognoisi of patients. Methods A randomized， controlled， prospective study was used in this study， 78 patients with ovarian endometriosis cyst were selected in Sichuan province panzhihua city miyi people's hospital from august 2012 to august 2014， and were randomly divided into observation group and control group， 39 patients in each group. Patients in the observation group were treated with laparoscopy combined with gestrinone （2.5 mg/time， two times per week， for 6 months） intervention; patients in the control group were treated with laparoscopic intervention. After six months， the postoperative serum FSH （Follicle Stmi Ulating Hormone， FSH）， Estradiol （E2） and Estradiol levels， ovarian biggest plane average diameter （D）， the level of MMP-9 and TIMP -1 level， pregnancy outcome of two groups were compared and the outcome and the recurrence rate 18 months after treatment were followed up. Results Six months after treatment， the E2 and FSH levels in the observation group were better than control group， with statistically significant difference （P＜0.01）；the postoperative serum levels of MMP-9 and TIMP-1 in Observation group were better than control group， with statistically significant difference （P＜0.01）; there were 20 patients of D≤2 cm， 19 patients of D>2 cm， the postoperative ovarian shrink rate was 51.28% in the observation group; there were 5 patients of D≤2 cm， 34 patients of D>2 cm ovarian shrink ratio was 12.82% in the control group， the ovarian shrink ratio of observation group was obviously higher than control group，with statistically significant difference （P＜0.01）. Follow-up for 18 months， the recurrence rate was 5.13% in the observation group，which was significantly lower than thatin the control group（25.64%），with statistically significant difference（P＜0.05）; the pregnancy rate was 66.66% and 30.77% in the Observation group and control group， respectively，the difference had statistical significance（P＜0.01）. Conclusion Laparoscopic surgery combined with gestrinone can protect ovarian function of patients with ovarian endometriosis cyst，improve pregnancy outcomes， and reduce recurrence rate， and has good clinical value.
［Abstract］Objective To study clinical effect of laparoscopy combined with gestrinone in treatment of ovarian endometriosis cyst and the prognoisi of patients. Methods A randomized， controlled， prospective study was used in this study， 78 patients with ovarian endometriosis cyst were selected in Sichuan province panzhihua city miyi people's hospital from august 2012 to august 2014， and were randomly divided into observation group and control group， 39 patients in each group. Patients in the observation group were treated with laparoscopy combined with gestrinone （2.5 mg/time， two times per week， for 6 months） intervention; patients in the control group were treated with laparoscopic intervention. After six months， the postoperative serum FSH （Follicle Stmi Ulating Hormone， FSH）， Estradiol （E2） and Estradiol levels， ovarian biggest plane average diameter （D）， the level of MMP-9 and TIMP -1 level， pregnancy outcome of two groups were compared and the outcome and the recurrence rate 18 months after treatment were followed up. Results Six months after treatment， the E2 and FSH levels in the observation group were better than control group， with statistically significant difference （P＜0.01）；the postoperative serum levels of MMP-9 and TIMP-1 in Observation group were better than control group， with statistically significant difference （P＜0.01）; there were 20 patients of D≤2 cm， 19 patients of D>2 cm， the postoperative ovarian shrink rate was 51.28% in the observation group; there were 5 patients of D≤2 cm， 34 patients of D>2 cm ovarian shrink ratio was 12.82% in the control group， the ovarian shrink ratio of observation group was obviously higher than control group，with statistically significant difference （P＜0.01）. Follow-up for 18 months， the recurrence rate was 5.13% in the observation group，which was significantly lower than thatin the control group（25.64%），with statistically significant difference（P＜0.05）; the pregnancy rate was 66.66% and 30.77% in the Observation group and control group， respectively，the difference had statistical significance（P＜0.01）. Conclusion Laparoscopic surgery combined with gestrinone can protect ovarian function of patients with ovarian endometriosis cyst，improve pregnancy outcomes， and reduce recurrence rate， and has good clinical value.
2016, 37(06)
Abstract:
［Abstract］Objective To investigate the feasibility and clinical effect of immediate ureterovesical reimplantation after ureteral rupture during laparoscopic hysterectomy. Methods From August 2010 to December 2015， 5 cases of ureteral rupture during the operation of laparoscopic hysterectomy were treated with immediate ureterovesical reimplantation under laparoscopy. Results All operations were successfully performed without transversion to open surgery. No patients with urinary leakage occurred. The mean follow-up were 21 months（range 3-60 months）. No cases with ureteral stricture were observed. Slight urine reflux was found in two patients，of whom obvious hydronephrosis and renal damage were not found. Conclusion Immediate ureterovesical reimplantation under laparoscopy is a feasible，safe and minimal invasive method for treatment of ureteral ruputure during laparoscopic hysterectomy.
［Abstract］Objective To investigate the feasibility and clinical effect of immediate ureterovesical reimplantation after ureteral rupture during laparoscopic hysterectomy. Methods From August 2010 to December 2015， 5 cases of ureteral rupture during the operation of laparoscopic hysterectomy were treated with immediate ureterovesical reimplantation under laparoscopy. Results All operations were successfully performed without transversion to open surgery. No patients with urinary leakage occurred. The mean follow-up were 21 months（range 3-60 months）. No cases with ureteral stricture were observed. Slight urine reflux was found in two patients，of whom obvious hydronephrosis and renal damage were not found. Conclusion Immediate ureterovesical reimplantation under laparoscopy is a feasible，safe and minimal invasive method for treatment of ureteral ruputure during laparoscopic hysterectomy.
2016, 37(06)
Abstract:
［Abstract］Objective To study the safety and effect of ultrasound guided minimally invasive rotary cutting for treatment of benign breast lesions. Methods A single-center， prospective， randomized， controlled study method was used， 84 patients with breast benign lesions were randomly divided into observation group and control group， 42 patients in each group. Patients in the observation group were treated with ultrasound guided minimally invasive rotary cutting， patients in control group were treated with traditional surgery. The intraoperative and postoperative related indicators were compared between two groups. Results The intraoperative blood loss， length of incision， operation time in breast benign unilateral single and unilateral and bilateral lesions in the observation group were significantly less than those in the control group， with statistically significant difference（P＜0.01）. The healing time， postoperative pain score， complications in breast benign unilateral single and unilateral and bilateral lesions in observation group were less than those in the control group， with statistically significant difference（P＜0.01）. And the postoperative satisfaction， and the excellent rate of beauty in breast benign unilateral single、unilateral and bilateral lesions were better than control group， with statistically significant difference（P＜0.01）. There was no breast deformation in observation group after treatment， and there were 3 patients of breast deformation in the control group has 3 patients， accounting for 7.14%. Conclusion Ultrasound guided minimally invasive rotary cutting is significantly superior to the traditional treatment in security and effectiveness for treatment of breast benign lesions in one single and unilateral multiple and bilateral lesions.
［Abstract］Objective To study the safety and effect of ultrasound guided minimally invasive rotary cutting for treatment of benign breast lesions. Methods A single-center， prospective， randomized， controlled study method was used， 84 patients with breast benign lesions were randomly divided into observation group and control group， 42 patients in each group. Patients in the observation group were treated with ultrasound guided minimally invasive rotary cutting， patients in control group were treated with traditional surgery. The intraoperative and postoperative related indicators were compared between two groups. Results The intraoperative blood loss， length of incision， operation time in breast benign unilateral single and unilateral and bilateral lesions in the observation group were significantly less than those in the control group， with statistically significant difference（P＜0.01）. The healing time， postoperative pain score， complications in breast benign unilateral single and unilateral and bilateral lesions in observation group were less than those in the control group， with statistically significant difference（P＜0.01）. And the postoperative satisfaction， and the excellent rate of beauty in breast benign unilateral single、unilateral and bilateral lesions were better than control group， with statistically significant difference（P＜0.01）. There was no breast deformation in observation group after treatment， and there were 3 patients of breast deformation in the control group has 3 patients， accounting for 7.14%. Conclusion Ultrasound guided minimally invasive rotary cutting is significantly superior to the traditional treatment in security and effectiveness for treatment of breast benign lesions in one single and unilateral multiple and bilateral lesions.
2016, 37(06)
Abstract:
［Abstract］Objective To compare the feasibility and efficacy between dexmedetomidine and midazolam in herniorrhaphy surgery for older patients. Methods Sixty American Society of Anesthesiology（ASA）grade Ⅰ～Ⅱpatients， treated by herniorrhaphy surgery under local anesthesia，were randomly divided into dexmedetomidine group（n＝30） and midazolam group（n＝30）.Patients in dexmedetomidine group were given dexmedetomidine at a loading dose of 1μg/kg for 10 min，then they were injected continuously by 0.4 μg/(kg·h)，whereas midazolam group were given midazolam at a loading dose of 0.06 mg/kg， then 0.04 mg/(kg·h) injected continuously.The mean blood pressure（MAP）and heart rate（HR） were recorded before infusion（T0），incision of skin（T1），15min（T2）and 30 min（T3） after administration and when sutured skin （T4），adverse reaction were also assessed. Results The difference of sedation level was not significant between the two groups（P＞0.05）.Compared with T0 ，the decrease of HR was significantly more in dexmedetomidine group from T1 to T4（P＜0.05）. Compared with midazolam group ， the decrease of HR was significantly more in dexmedetomidine group from T1 to T4（P＜0.05）. Compared with dexmedetomidine group，the rate of respiratory depression and restlessness were more in midazolam group，but bradycardia was lower（P＜0.05）.Conclusions Dexmedetomidine is a comparable alternative to midazolam for sedation in herniorrhaphy surgery under local anesthesia. It is associated with better respiration and lower restlessness but with a high incidence of bradycardia.
［Abstract］Objective To compare the feasibility and efficacy between dexmedetomidine and midazolam in herniorrhaphy surgery for older patients. Methods Sixty American Society of Anesthesiology（ASA）grade Ⅰ～Ⅱpatients， treated by herniorrhaphy surgery under local anesthesia，were randomly divided into dexmedetomidine group（n＝30） and midazolam group（n＝30）.Patients in dexmedetomidine group were given dexmedetomidine at a loading dose of 1μg/kg for 10 min，then they were injected continuously by 0.4 μg/(kg·h)，whereas midazolam group were given midazolam at a loading dose of 0.06 mg/kg， then 0.04 mg/(kg·h) injected continuously.The mean blood pressure（MAP）and heart rate（HR） were recorded before infusion（T0），incision of skin（T1），15min（T2）and 30 min（T3） after administration and when sutured skin （T4），adverse reaction were also assessed. Results The difference of sedation level was not significant between the two groups（P＞0.05）.Compared with T0 ，the decrease of HR was significantly more in dexmedetomidine group from T1 to T4（P＜0.05）. Compared with midazolam group ， the decrease of HR was significantly more in dexmedetomidine group from T1 to T4（P＜0.05）. Compared with dexmedetomidine group，the rate of respiratory depression and restlessness were more in midazolam group，but bradycardia was lower（P＜0.05）.Conclusions Dexmedetomidine is a comparable alternative to midazolam for sedation in herniorrhaphy surgery under local anesthesia. It is associated with better respiration and lower restlessness but with a high incidence of bradycardia.
2016, 37(06)
Abstract:
［Abstract］Objective To observe the effect of Bacillus coagulans tablets Combined with minus Xiaoyao powder， Nifuratel nystatin soft capsule in treatment of VVC. Methods A total of 108 cases of VVC. patients were randomly divided into 2 groups: observation group and control group. In the observation group （n = 54）， patients were given vaginal placement of Nifuratel nystatin soft capsule， 1 capsule daily， for 6 days; oral administration of minus Xiaoyao powder，1 agent daily，for 7 days; and oral administration of Bacillus coagulans tablets， 3 tablets one time， 3 times a day，for 14 days， double dose at first. In control group （54 cases）， patients were given vaginal placement of Nifuratel nystatin soft capsule， 1 capsule daily， for 6 days; and oral placement of vitamin C tablet， 1 tablet one time， 3 times a day，for 14 days. The short-term effect and the long-term recurrence of were observed 3-7 days， 1 months， 3 months， 6 months after stopping drug treatment. Results 3 days after stopping drugs， the effective rate in the observation group and the control group was 92.59% and 81.48%， respectively， the difference between the two groups was statistically significant（P＜0.05）. 6 months after stopping drugs， the recurrence rate was 9.26% in the observation group， and 20.37% in the control group， the difference was statistically significant（P＜0.05）. Conclusion For Vaginal candidiasis， on the basis of antifungal therapy，giving Bacillus coagulans tablets for regulation of intestinal micro ecological environment， and traditional Chinese medicine minus Xiaoyao powder for clearing heat and removing dampness，soothing the liver，can significantly improve the short term and long term curative effects，and reduce the recurrence rate.
［Abstract］Objective To observe the effect of Bacillus coagulans tablets Combined with minus Xiaoyao powder， Nifuratel nystatin soft capsule in treatment of VVC. Methods A total of 108 cases of VVC. patients were randomly divided into 2 groups: observation group and control group. In the observation group （n = 54）， patients were given vaginal placement of Nifuratel nystatin soft capsule， 1 capsule daily， for 6 days; oral administration of minus Xiaoyao powder，1 agent daily，for 7 days; and oral administration of Bacillus coagulans tablets， 3 tablets one time， 3 times a day，for 14 days， double dose at first. In control group （54 cases）， patients were given vaginal placement of Nifuratel nystatin soft capsule， 1 capsule daily， for 6 days; and oral placement of vitamin C tablet， 1 tablet one time， 3 times a day，for 14 days. The short-term effect and the long-term recurrence of were observed 3-7 days， 1 months， 3 months， 6 months after stopping drug treatment. Results 3 days after stopping drugs， the effective rate in the observation group and the control group was 92.59% and 81.48%， respectively， the difference between the two groups was statistically significant（P＜0.05）. 6 months after stopping drugs， the recurrence rate was 9.26% in the observation group， and 20.37% in the control group， the difference was statistically significant（P＜0.05）. Conclusion For Vaginal candidiasis， on the basis of antifungal therapy，giving Bacillus coagulans tablets for regulation of intestinal micro ecological environment， and traditional Chinese medicine minus Xiaoyao powder for clearing heat and removing dampness，soothing the liver，can significantly improve the short term and long term curative effects，and reduce the recurrence rate.
2016, 37(06)
Abstract:
［Abstract］Objective To investigate the clinical value of blood β-hydroxybutyric acid and urine ketone in the diagnosis of diabetic ketosis. Methods One hundred and fifteen patients with non-ketosis diabetics （NDK group）， 85 patients with diabetic ketosis（DK group）and 105 healthy people（control group）were enrolled in Chengmai People's Hospital from May 2012 to May 2014. Blood β-hydroxybutyric acid，urine ketone and blood glucose were detected; the correlation index of blood β-hydroxybutyric acid，urine ketone and blood glucose in NDK group and DK group were analyzed. The receiver operating characteristic curve（ROC）was performed in determining the diagnostic value of blood β-hydroxybutyric acid for diabetic ketosis. Results The level of blood β-hydroxybutyric acid， positive rate of blood β-hydroxybutyric acid， urine ketone and level of blood glucose in DK group were higher than those in NDK group and NC group （P＜0.01）. Blood β-hydroxybutyric acid was positively correlated with urine ketone and blood glucose （r＝0.552，P＝0.000；r＝0.405，P＝0.010）. When urine ketone was used as diagnostic standard， the area under curve of blood β-hydroxybutyric acid was 0.839， the best cutoff value of β-hydroxybutyric acid was 0.64 mmol/L with the sensitivity was 81.6% and specificity was 89.2%. Conclusions Examinations of blood β-hydroxybutyric acid and urine ketone have great significance for the diagnosis of diabetic ketosis. The co-monitoring of blood β-hydroxybutyric acid and urine ketone can reduce the rates of missed diagnosis and misdiagnosis. The best cutoff value of β-hydroxybutyric acid to diagnose diabetic ketosis was 0.64 mmol/L.
［Abstract］Objective To investigate the clinical value of blood β-hydroxybutyric acid and urine ketone in the diagnosis of diabetic ketosis. Methods One hundred and fifteen patients with non-ketosis diabetics （NDK group）， 85 patients with diabetic ketosis（DK group）and 105 healthy people（control group）were enrolled in Chengmai People's Hospital from May 2012 to May 2014. Blood β-hydroxybutyric acid，urine ketone and blood glucose were detected; the correlation index of blood β-hydroxybutyric acid，urine ketone and blood glucose in NDK group and DK group were analyzed. The receiver operating characteristic curve（ROC）was performed in determining the diagnostic value of blood β-hydroxybutyric acid for diabetic ketosis. Results The level of blood β-hydroxybutyric acid， positive rate of blood β-hydroxybutyric acid， urine ketone and level of blood glucose in DK group were higher than those in NDK group and NC group （P＜0.01）. Blood β-hydroxybutyric acid was positively correlated with urine ketone and blood glucose （r＝0.552，P＝0.000；r＝0.405，P＝0.010）. When urine ketone was used as diagnostic standard， the area under curve of blood β-hydroxybutyric acid was 0.839， the best cutoff value of β-hydroxybutyric acid was 0.64 mmol/L with the sensitivity was 81.6% and specificity was 89.2%. Conclusions Examinations of blood β-hydroxybutyric acid and urine ketone have great significance for the diagnosis of diabetic ketosis. The co-monitoring of blood β-hydroxybutyric acid and urine ketone can reduce the rates of missed diagnosis and misdiagnosis. The best cutoff value of β-hydroxybutyric acid to diagnose diabetic ketosis was 0.64 mmol/L.
2016, 37(06)
Abstract:
［Abstract］Objective To investigate the changes of urinary β2-microglobulin （β2-MG）， retinol conjugated protein （RBP）， N-acetyl-beta-D nucleosidase amino acids （NAG） and serum cystatin C （Cys C） in patients with primary nephrotic syndrome （PNS）， and explore the application value of those indexes in PNS diagnosis. Methods Fifty-three patients with PNS from Sep. 2009 to Sep. 2015 were enrolled in this study， and 40 healthy people were selected as the control group. The levels of β2-MG， RBP， NAG in urinary and Cys C in serum were measured in two groups， and the receiver operator characteristic curves （ROC） were drawn out for investigating the diagnostic value of four indexes to PNS. Results The levels of β2-MG， RBP， NAG in urinary and Cys C in serum of patients with PNS were significantly higher than control group （P＜0.01）. The RBP， NAG and Cys C had the higher efficiency in diagnosis PNS than β2-MG， the differences were statistically significant （P＜0.05）. There were no significant differences in diagnosis PNS between RBP， NAG and Cys C （P＞0.05）. The area of RBP under the ROC curve was 0.897（95%CI was 0.807-0.987）bigger than NAG， Cys C and β2-MG. Conclusion β2-MG， RBP， NAG in urinary and Cys C in serum have high sensitivity and specificity in diagnosis PNS， monitoring the levels of β2-MG，RBP，NAG in urinary and Cys C in serum may be helpful for diagnosis and clinical therapy of PNS.
［Abstract］Objective To investigate the changes of urinary β2-microglobulin （β2-MG）， retinol conjugated protein （RBP）， N-acetyl-beta-D nucleosidase amino acids （NAG） and serum cystatin C （Cys C） in patients with primary nephrotic syndrome （PNS）， and explore the application value of those indexes in PNS diagnosis. Methods Fifty-three patients with PNS from Sep. 2009 to Sep. 2015 were enrolled in this study， and 40 healthy people were selected as the control group. The levels of β2-MG， RBP， NAG in urinary and Cys C in serum were measured in two groups， and the receiver operator characteristic curves （ROC） were drawn out for investigating the diagnostic value of four indexes to PNS. Results The levels of β2-MG， RBP， NAG in urinary and Cys C in serum of patients with PNS were significantly higher than control group （P＜0.01）. The RBP， NAG and Cys C had the higher efficiency in diagnosis PNS than β2-MG， the differences were statistically significant （P＜0.05）. There were no significant differences in diagnosis PNS between RBP， NAG and Cys C （P＞0.05）. The area of RBP under the ROC curve was 0.897（95%CI was 0.807-0.987）bigger than NAG， Cys C and β2-MG. Conclusion β2-MG， RBP， NAG in urinary and Cys C in serum have high sensitivity and specificity in diagnosis PNS， monitoring the levels of β2-MG，RBP，NAG in urinary and Cys C in serum may be helpful for diagnosis and clinical therapy of PNS.
2016, 37(06)
Abstract:
［Abstract］Objective To investigate the changes of serum C reactive protein （CRP）， Interleukin-2 （IL-2）， Monocyte chemoattractant protein-1（MCP-1）and hemorheology in patients with pelvic inflammatory disease. Methods Eighty patients with pelvic inflammatory disease were enrolled as observation group from May 2013 to May 2015， 60 healthy women in the same age were selected as control group at the same period. The serum CRP， IL-2， MCP-1 and hemorheology were compared between observation group and control group; the detection indexes were compared in patients with mild， moderate and severe pelvic inflammatory disease in observation group. Results The serum levels of CRP and MCP-1 and hemorheology of observation group were higher than those of control group， the differences were statistically significant（P＜0.05）. The detection indexes of patients with severe pelvic inflammatory disease were higher than those of mild and moderate pelvic inflammatory disease， the detection indexes of patients with moderate pelvic inflammatory disease were higher than those of mild pelvic inflammatory disease， the differences were all statistically significant （P＜0.05）. The level of IL-2 of was significantly lower than that of control group， the level of IL-2 of patients with severe pelvic inflammatory disease was lower than that of mild and moderate pelvic inflammatory disease， the level of IL-2 of patients with moderate pelvic inflammatory disease was significantly lower than that of mild pelvic inflammatory disease， the differences were all statistically significant（P＜0.05）. Conclusion The serum levels of CRP，IL-2，MCP-1 and hemorheology of patients with pelvic inflammatory disease have significant changes， and the detection indexes of patients with mild， moderate and severe pelvic inflammatory disease have significant differences.
［Abstract］Objective To investigate the changes of serum C reactive protein （CRP）， Interleukin-2 （IL-2）， Monocyte chemoattractant protein-1（MCP-1）and hemorheology in patients with pelvic inflammatory disease. Methods Eighty patients with pelvic inflammatory disease were enrolled as observation group from May 2013 to May 2015， 60 healthy women in the same age were selected as control group at the same period. The serum CRP， IL-2， MCP-1 and hemorheology were compared between observation group and control group; the detection indexes were compared in patients with mild， moderate and severe pelvic inflammatory disease in observation group. Results The serum levels of CRP and MCP-1 and hemorheology of observation group were higher than those of control group， the differences were statistically significant（P＜0.05）. The detection indexes of patients with severe pelvic inflammatory disease were higher than those of mild and moderate pelvic inflammatory disease， the detection indexes of patients with moderate pelvic inflammatory disease were higher than those of mild pelvic inflammatory disease， the differences were all statistically significant （P＜0.05）. The level of IL-2 of was significantly lower than that of control group， the level of IL-2 of patients with severe pelvic inflammatory disease was lower than that of mild and moderate pelvic inflammatory disease， the level of IL-2 of patients with moderate pelvic inflammatory disease was significantly lower than that of mild pelvic inflammatory disease， the differences were all statistically significant（P＜0.05）. Conclusion The serum levels of CRP，IL-2，MCP-1 and hemorheology of patients with pelvic inflammatory disease have significant changes， and the detection indexes of patients with mild， moderate and severe pelvic inflammatory disease have significant differences.
2016, 37(06)
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［Abstract］Objective To analyze the concentration changes of insulin and c peptide of in portal vein and peripheral blood， and explore the feasibility of using insulin and c peptide concentration changes in assessment of liver function. Methods The portal vein and peripheral blood samples were extracted from 82 clinical liver cancer patients，and the insulin and c peptide concentration were detected. The differences in the insulin and c peptide concentration in portal vein and peripheral blood were statistically analyzed. Results The insulin concentration in the portal vein and peripheral blood values were 32.43 μ U/ml and 8.05 μ U/ml， respectively， there was a significant difference（P＜0.05）. The c-peptide concentration in the portal vein and peripheral blood values were 1.38 μU/mL and 1.19 μg U/mL， respectively， there was no significant differences（P＞0.05）. Conclusion Liver metabolizes insulin as the target organ of insulin， while c-peptide dose not be metabolized in vivo， so it is possible to assess liver function by comparative analysis of the metabolic rate of insulin and c-peptide changes.
［Abstract］Objective To analyze the concentration changes of insulin and c peptide of in portal vein and peripheral blood， and explore the feasibility of using insulin and c peptide concentration changes in assessment of liver function. Methods The portal vein and peripheral blood samples were extracted from 82 clinical liver cancer patients，and the insulin and c peptide concentration were detected. The differences in the insulin and c peptide concentration in portal vein and peripheral blood were statistically analyzed. Results The insulin concentration in the portal vein and peripheral blood values were 32.43 μ U/ml and 8.05 μ U/ml， respectively， there was a significant difference（P＜0.05）. The c-peptide concentration in the portal vein and peripheral blood values were 1.38 μU/mL and 1.19 μg U/mL， respectively， there was no significant differences（P＞0.05）. Conclusion Liver metabolizes insulin as the target organ of insulin， while c-peptide dose not be metabolized in vivo， so it is possible to assess liver function by comparative analysis of the metabolic rate of insulin and c-peptide changes.
2016, 37(06)
Abstract:
［Abstract］Objective To investigate the clinical characteristics of hodgkin's lymphoma（HL） patients . Method The clinical data of HL patients in our hospital from January 1, 2003 to December 31, 2012 were analyzed retrospectively. The contents of investigation included gender, age, pathological type, first symptom, the incidence locations, clinical stages, extranodal involvement locations, ECOG and treatments．Results A total of 143 cases of HL were included， the M/F ratio was 2.5:1 with a median age of 37 years （11~73years）. The most frequent subtypes of HL were mixed cellular （34.3%） and nodular sclerosis（28.0%）. The nodal lymphomas accounted for 97.2%， while the extranodal lymphomas occupied 2.1%. The nodal lymphomas with extranodal invasion accounted for 29.5%，and the common locations were spleen（14.4%），lung（5.8%）and bone（5.0%）. The most clinical stage of all patients was stage Ⅰ/Ⅱ（60.8%），The differences were no significant in gender, pathological type，first symptom，the incidence locations and the effective rate of the first-line treatment（P＞0.05），while were statistically significant in B symptoms，ECOG and treatments between Ⅰ/Ⅱgroups and Ⅲ/Ⅳ groups（P＜0.05）. Conclusions In this group，the incidence of HL patients were mainly found in middle-aged people，males were lager than females.The pathological type，first symptom and the incidence locations had no obvious effect on clinical stage. The clinical stage had no obvious influence on the effective rate of the first-line treatment.
［Abstract］Objective To investigate the clinical characteristics of hodgkin's lymphoma（HL） patients . Method The clinical data of HL patients in our hospital from January 1, 2003 to December 31, 2012 were analyzed retrospectively. The contents of investigation included gender, age, pathological type, first symptom, the incidence locations, clinical stages, extranodal involvement locations, ECOG and treatments．Results A total of 143 cases of HL were included， the M/F ratio was 2.5:1 with a median age of 37 years （11~73years）. The most frequent subtypes of HL were mixed cellular （34.3%） and nodular sclerosis（28.0%）. The nodal lymphomas accounted for 97.2%， while the extranodal lymphomas occupied 2.1%. The nodal lymphomas with extranodal invasion accounted for 29.5%，and the common locations were spleen（14.4%），lung（5.8%）and bone（5.0%）. The most clinical stage of all patients was stage Ⅰ/Ⅱ（60.8%），The differences were no significant in gender, pathological type，first symptom，the incidence locations and the effective rate of the first-line treatment（P＞0.05），while were statistically significant in B symptoms，ECOG and treatments between Ⅰ/Ⅱgroups and Ⅲ/Ⅳ groups（P＜0.05）. Conclusions In this group，the incidence of HL patients were mainly found in middle-aged people，males were lager than females.The pathological type，first symptom and the incidence locations had no obvious effect on clinical stage. The clinical stage had no obvious influence on the effective rate of the first-line treatment.
2016, 37(06)
Abstract:
［Abstract］Objective Analysis of clinical features and treatment of nasal type NK/T cell lymphoma, to improve the understanding of this special type of lymphoma．Method The clinical data of 34 patients with nasal type NK/T cell lymphoma confirmed by pathology were retrospectively analyzed．Result In 31 cases，26 cases were EB virus（EBV）infection positive，recent Treatment Effectiveness in the positive group was significantly lower than the negative group（38.46% vs. 87.50%；P＜0.05）. Follow up showed until October 2015, 19 of 34 patients died. Among them, 17 cases died of disease relapse or progression, 2 cases died of other diseases. 15 patients survived (11 of them were disease-free) and the median overall survival time was 37 months. The 2 year overall survival was related to clinical stage, B symptoms, EBV infection（the P values were 0.017/0.001，0.005，and 0.039 respectively)．Conclusion EBV infection is associated with patient's treatment response in NK/T-Cell Lymphoma; and the prognosis is associated with clinical features such as clinical stage, B symptom，and EBV infection.
［Abstract］Objective Analysis of clinical features and treatment of nasal type NK/T cell lymphoma, to improve the understanding of this special type of lymphoma．Method The clinical data of 34 patients with nasal type NK/T cell lymphoma confirmed by pathology were retrospectively analyzed．Result In 31 cases，26 cases were EB virus（EBV）infection positive，recent Treatment Effectiveness in the positive group was significantly lower than the negative group（38.46% vs. 87.50%；P＜0.05）. Follow up showed until October 2015, 19 of 34 patients died. Among them, 17 cases died of disease relapse or progression, 2 cases died of other diseases. 15 patients survived (11 of them were disease-free) and the median overall survival time was 37 months. The 2 year overall survival was related to clinical stage, B symptoms, EBV infection（the P values were 0.017/0.001，0.005，and 0.039 respectively)．Conclusion EBV infection is associated with patient's treatment response in NK/T-Cell Lymphoma; and the prognosis is associated with clinical features such as clinical stage, B symptom，and EBV infection.
2016, 37(06)
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［Abstract］Objective Through clinical observation and statistics，to get the best curative effect of surgical operation in treating pigment nevus method and provide clinical guidance. Methods We reviewed of face and neck patients (1100 patients) with pigmented nevus in the department in the department of the dermatology，plastic surgery from January 2013 to October 2015，two different methods was designed on each parts and effect of the treatment, especially satisfaction degree was analyzed by statistical methods．Results In 100 cases of patients, only 20 patients had mild scar hyperplasia at the neck incision and the rest of the patients were satisfactory. For special parts such as mouth，nose and eye around，along thedirection of the muscle, arc and along the direction of dermatoglyph incision was designed respectively, patients obtained with higher postoperative satisfaction（P < 0.05）. Conclusion In pigmented nevus of face and neck surgery treatment, surgical incision design requires dynamic and static combining method，incision design is important for the postoperative effect and patients' satisfaction.
［Abstract］Objective Through clinical observation and statistics，to get the best curative effect of surgical operation in treating pigment nevus method and provide clinical guidance. Methods We reviewed of face and neck patients (1100 patients) with pigmented nevus in the department in the department of the dermatology，plastic surgery from January 2013 to October 2015，two different methods was designed on each parts and effect of the treatment, especially satisfaction degree was analyzed by statistical methods．Results In 100 cases of patients, only 20 patients had mild scar hyperplasia at the neck incision and the rest of the patients were satisfactory. For special parts such as mouth，nose and eye around，along thedirection of the muscle, arc and along the direction of dermatoglyph incision was designed respectively, patients obtained with higher postoperative satisfaction（P < 0.05）. Conclusion In pigmented nevus of face and neck surgery treatment, surgical incision design requires dynamic and static combining method，incision design is important for the postoperative effect and patients' satisfaction.
2016, 37(06)
Abstract:
［Abstract］Objective To study the value of fiberoptic bronchoscopy in treatment of ventilator associated pneumonia. Methods The 168 cases of ventilator associated pneumonia in ICU were randomly divided into treatment group and control group， each group of 84 cases， were given oxygen， anti-inflammatory， expectorant treatment. Treatment group was given fiber bronchoscope sputum suction and irrigation， control group was given sputum suction tube， then we observed the oxygenation changes after sputum suction of two groups of patients， compared clinical efficacy， sputum culture positive rate， antibiotic using time and staying time in the ICU between two groups of patients. Results Compared with control group， in treatment group SaO2， PaO2 and oxygen index were significantly improved （P < 0.05）; in treatment group CRP，PCT and IL-6 were significantly improved （P < 0.05）. In the treatment group， the total effectiveness is significantly higher than the control group; antibiotic using time and staying time of ICU were obviously shorter than the control group （P < 0.05）. Conclusion Fiberoptic bronchoscopy irrigation treatment can improve oxygenation， antibiotic using time and staying time in ICU of ventilator associated pneumonia patients， and is worthy of clinical application.
［Abstract］Objective To study the value of fiberoptic bronchoscopy in treatment of ventilator associated pneumonia. Methods The 168 cases of ventilator associated pneumonia in ICU were randomly divided into treatment group and control group， each group of 84 cases， were given oxygen， anti-inflammatory， expectorant treatment. Treatment group was given fiber bronchoscope sputum suction and irrigation， control group was given sputum suction tube， then we observed the oxygenation changes after sputum suction of two groups of patients， compared clinical efficacy， sputum culture positive rate， antibiotic using time and staying time in the ICU between two groups of patients. Results Compared with control group， in treatment group SaO2， PaO2 and oxygen index were significantly improved （P < 0.05）; in treatment group CRP，PCT and IL-6 were significantly improved （P < 0.05）. In the treatment group， the total effectiveness is significantly higher than the control group; antibiotic using time and staying time of ICU were obviously shorter than the control group （P < 0.05）. Conclusion Fiberoptic bronchoscopy irrigation treatment can improve oxygenation， antibiotic using time and staying time in ICU of ventilator associated pneumonia patients， and is worthy of clinical application.
2016, 37(06)
Abstract:
［Abstract］Human rotavirus has been discovered since the early 1970s，which is still a primary pathogen causing infant diarrhea under the age of five. Each year about 600，000 infant died of severe body dehydration caused by rotavirus infection. Since the current rotavirus diarrhea have no specific therapeutic drugs，using vaccine to prevent rotavirus infection is an effective method. Research on a vaccine often involved in a multidisciplinary theory and technology. This article reviewed the epidemic situation of rotavirus，animal model，the body's immune response after infection， developing and using vaccine and other related issues.
［Abstract］Human rotavirus has been discovered since the early 1970s，which is still a primary pathogen causing infant diarrhea under the age of five. Each year about 600，000 infant died of severe body dehydration caused by rotavirus infection. Since the current rotavirus diarrhea have no specific therapeutic drugs，using vaccine to prevent rotavirus infection is an effective method. Research on a vaccine often involved in a multidisciplinary theory and technology. This article reviewed the epidemic situation of rotavirus，animal model，the body's immune response after infection， developing and using vaccine and other related issues.
2016, 37(06)
Abstract:
［Abstract］Objective To explore the effect of follow-up continuous nursing intervention on the psychological quality and medication compliance in patients with ulcerative colitis after discharge. Methods Ninety-seven patients with ulcerative colitis after discharge from October 2014 to October 2015 were recruited，and 48 cases were selected in the study group and 49 cases in the control group randomly. The control group received routine postdischarge care. The study group received postdischarge follow-up nursing intervention. After 3 months of postdischarge intervention，the psychological quality of patients in two groups was assessed by the emotion application measure scale separately.The Morisky Medication Adherence Scale 8-item version（MMAS 8-item version） was used for the assessment of the medication compliance of the two groups of patients. Results After the intervention， the patients in the study group showed better psychological quality and medication compliance than the patients in the control groups.There were significant differences between two groups. Conclusion Post-discharge follow-up nursing intervention on patients with ulcerative colitis is effective for improving the psychological quality and the medication compliance and may be helpful to improve the life quality of the patients.
［Abstract］Objective To explore the effect of follow-up continuous nursing intervention on the psychological quality and medication compliance in patients with ulcerative colitis after discharge. Methods Ninety-seven patients with ulcerative colitis after discharge from October 2014 to October 2015 were recruited，and 48 cases were selected in the study group and 49 cases in the control group randomly. The control group received routine postdischarge care. The study group received postdischarge follow-up nursing intervention. After 3 months of postdischarge intervention，the psychological quality of patients in two groups was assessed by the emotion application measure scale separately.The Morisky Medication Adherence Scale 8-item version（MMAS 8-item version） was used for the assessment of the medication compliance of the two groups of patients. Results After the intervention， the patients in the study group showed better psychological quality and medication compliance than the patients in the control groups.There were significant differences between two groups. Conclusion Post-discharge follow-up nursing intervention on patients with ulcerative colitis is effective for improving the psychological quality and the medication compliance and may be helpful to improve the life quality of the patients.
2016, 37(06)
Abstract:
［Abstract］Objective To analyze the effect of Evidence-based Care on the complication rate and comfort in patients with coronary heart disease after intervention therapy， and to provide reference for clinical care. Methods One hundred and twenty-two patients with interventional treatment of coronary heart disease in hospital from May 2013 to April 2015 were selected， patients were divided into control group（61 cases） and intervention group（61 cases） according to the care methods. Control group patients were given careful routine care， Intervention group patients were given Evidence-based Care，the complication rate and comfort of the two groups were compared. Results The incidence of postoperative complications in the intervention group （11.5%） was significantly lower than the control group （76.6%），P＜0.05. The intervention group after 6h （61.5 ± 5.3）， 12h （70.2 ± 4.5），24h （87.5 ± 3.5） comfort were significantly higher than control group 6h （52.6 ± 6.8）， 12h （61.2 ± 5.3），24h （73.5 ± 4.2），P＜0.05. The incidence of bad mood of intervention group were significantly lower than the control group，P＜0.05. The intervention group patient care satisfaction （96.7%） was significantly higher than control group （86.9 %）， P <0.05. Conclusion Evidence-based nursing for the patients with coronary heart disease undergoing PCI can greatly decrease vascular complications and improve comfort value.
［Abstract］Objective To analyze the effect of Evidence-based Care on the complication rate and comfort in patients with coronary heart disease after intervention therapy， and to provide reference for clinical care. Methods One hundred and twenty-two patients with interventional treatment of coronary heart disease in hospital from May 2013 to April 2015 were selected， patients were divided into control group（61 cases） and intervention group（61 cases） according to the care methods. Control group patients were given careful routine care， Intervention group patients were given Evidence-based Care，the complication rate and comfort of the two groups were compared. Results The incidence of postoperative complications in the intervention group （11.5%） was significantly lower than the control group （76.6%），P＜0.05. The intervention group after 6h （61.5 ± 5.3）， 12h （70.2 ± 4.5），24h （87.5 ± 3.5） comfort were significantly higher than control group 6h （52.6 ± 6.8）， 12h （61.2 ± 5.3），24h （73.5 ± 4.2），P＜0.05. The incidence of bad mood of intervention group were significantly lower than the control group，P＜0.05. The intervention group patient care satisfaction （96.7%） was significantly higher than control group （86.9 %）， P <0.05. Conclusion Evidence-based nursing for the patients with coronary heart disease undergoing PCI can greatly decrease vascular complications and improve comfort value.
2016, 37(06)
Abstract:
［Abstract］Objective To explore the clinical effect of health education and psychological nursing intervention on the prognosis of patients with postpartum depression. Methods Ninety cases of postpartum depression in our hospital from March 2012 to March 2014， were randomly divided into the control group and the observation group， 45 cases in each group. The patients in the control group were given routine nursing， the patients in the observation group received health education and psychological intervention on the basis of the control group. The anxiety and depression scores change，medication adherence and quality of life in the two groups were observed and compared. Results The scores of SDS and SAS in the observation group were significantly lower than those in the control group （P＜0.05）. The medication adherence and quality of life score 3 months and 6 months after discharge in the observation group were significantly higher than those of control group（P＜0.05）. Conclusion Health education and psychological intervention has positive effect on the prognosis of postpartum depression patients， and can effectively improve the patient's medication compliance and quality of life， it's one of the effective measures for the prevention and treatment of postpartum depression.
［Abstract］Objective To explore the clinical effect of health education and psychological nursing intervention on the prognosis of patients with postpartum depression. Methods Ninety cases of postpartum depression in our hospital from March 2012 to March 2014， were randomly divided into the control group and the observation group， 45 cases in each group. The patients in the control group were given routine nursing， the patients in the observation group received health education and psychological intervention on the basis of the control group. The anxiety and depression scores change，medication adherence and quality of life in the two groups were observed and compared. Results The scores of SDS and SAS in the observation group were significantly lower than those in the control group （P＜0.05）. The medication adherence and quality of life score 3 months and 6 months after discharge in the observation group were significantly higher than those of control group（P＜0.05）. Conclusion Health education and psychological intervention has positive effect on the prognosis of postpartum depression patients， and can effectively improve the patient's medication compliance and quality of life， it's one of the effective measures for the prevention and treatment of postpartum depression.
