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LINC00341通过MAPK通路抑制肺腺癌细胞增殖

马诗淇 丁毅 李敏 王梦慈 张思宇 冯树梅

马诗淇, 丁毅, 李敏, 王梦慈, 张思宇, 冯树梅. LINC00341通过MAPK通路抑制肺腺癌细胞增殖[J]. 昆明医科大学学报, 2023, 44(4): 1-8. doi: 10.12259/j.issn.2095-610X.S20230403
引用本文: 马诗淇, 丁毅, 李敏, 王梦慈, 张思宇, 冯树梅. LINC00341通过MAPK通路抑制肺腺癌细胞增殖[J]. 昆明医科大学学报, 2023, 44(4): 1-8. doi: 10.12259/j.issn.2095-610X.S20230403
Shiqi MA, Yi DING, Min LI, Mengci WANG, Siyu ZHANG, Shumei FENG. LINC00341 Inhibits the Proliferation of Lung Adenocarcinoma Cells Through the MAPK Pathway[J]. Journal of Kunming Medical University, 2023, 44(4): 1-8. doi: 10.12259/j.issn.2095-610X.S20230403
Citation: Shiqi MA, Yi DING, Min LI, Mengci WANG, Siyu ZHANG, Shumei FENG. LINC00341 Inhibits the Proliferation of Lung Adenocarcinoma Cells Through the MAPK Pathway[J]. Journal of Kunming Medical University, 2023, 44(4): 1-8. doi: 10.12259/j.issn.2095-610X.S20230403

LINC00341通过MAPK通路抑制肺腺癌细胞增殖

doi: 10.12259/j.issn.2095-610X.S20230403
基金项目: 国家自然科学基金资助项目(81660324);新疆维吾尔自治区自然科学基金资助项目(2021D01C267)
详细信息
    作者简介:

    马诗淇(1998~),女,陕西渭南人,在读硕士研究生,主要从事肿瘤发病机制研究工作

    通讯作者:

    冯树梅, E-mail: 87391167@qq.com

  • 中图分类号: R734

LINC00341 Inhibits the Proliferation of Lung Adenocarcinoma Cells Through the MAPK Pathway

  • 摘要:   目的   探讨LINC00341对肺腺癌细胞增殖的影响及其潜在机制。   方法   在公共数据集SEO和TCGA中分析肺腺癌中LINC00341 mRNA的表达,ROC曲线分析其诊断价值,对RMA和OKAYAMA数据进行生存分析,并通过美国密歇根大学医院肺癌样本进行RNA-seq验证和ROC曲线分析;结合基因本体(gene otology,GO)分析LINC00341基因生物学功能;通过siRNA沉默LINC00341的表达,根据不同处理分为对照组(NT)和沉默组(siLINC00341),并验证细胞转染效率;采用WST-1和平板克隆实验检测细胞增殖能力;RNA亚细胞定位检测LINC00341定位,Western blotting检测其对MAPK信号通路相关蛋白表达水平的影响。   结果   LINC00341在肺腺癌组织中低表达(3个数据集P < 0.0001),ROC曲线分析表明其可作为肺腺癌的诊断标记物,同时低表达LINC00341与患者较好的预后相关( P = 0.004)。GO富集分析结果表明LINC00341主要与RNA聚合酶II启动子转录调控、蛋白质结合等有关。沉默LINC00341可以促进肺腺癌细胞的增殖(P < 0.05)和克隆形成( P < 0.05),且MAPK信号通路蛋白STAT3和ERK均被激活( P < 0.05),RNA定位显示LINC00341主要分布在细胞核。   结论   LINC00341可能在转录水平调控MAPK通路,进而抑制肺腺癌发展,并可作为肺腺癌重要的诊断和预后标志物。
  • 图  1  LINC00341 在肺腺癌细胞中的表达、ROC曲线以及生存分析

    A~C:UM,SEO和TCGA RNA测序数据集中,LUAD和正常肺组织样本中LINC00341表达水平散点图(3个数据集均P < 0.0001);D~F:LINC00341在UM,SEO和TCGA 3个数据集中AUC值的ROC曲线;G~H:Kaplan-Meier曲线对LINC00341的生存分析。P < 0.05。

    Figure  1.  Expression,ROC curve and survival of LINC00341 in lung adenocarcinoma cell

    图  2  LINC00341的GO富集分析

    A:靶基因生物学过程(BP)功能气泡图;B:靶基因分子功能(MF)功能气泡图。

    Figure  2.  GO enrichment analysis of LINC00341

    图  3  沉默LINC00341对PC-9细胞增殖能力的影响

    A:qRT-PCR检测转染效率;B:WST-1方法检测LINC00341沉默后细胞增殖能力;C~D:平板克隆实验检测LINC00341后细胞克隆形成能力。**P < 0.01,**** P < 0.0001。

    Figure  3.  Effect of silencing LINC00341 on proliferation ability of PC-9 cell

    图  4  沉默LINC00341对通路蛋白的影响以及LINC00341的亚细胞定位

    A:qRT-PCR显示LINC00341在肺腺癌细胞中的核和细胞质部分;B:Western blot检测LINC00341沉默后STAT3(信号传导及转录激酶蛋白),ERK1/2(细胞外调节蛋白激酶)和P38(丝裂原活化蛋白激酶)蛋白表达情况;C:各个目的蛋白相对表达水平。*P < 0.05,**P < 0.01,***P < 0.001。

    Figure  4.  Effect of silencing LINC00341 on proteins and subcellular localization of LINC00341

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  • 收稿日期:  2022-12-23
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