Effect of Compound Probiotic Powder on Immune Function of Mice
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摘要:
目的 探究复合益生菌粉在增强机体相关免疫功能的作用，以期为未来更深入地探索和应用该复合益生菌粉提供理论依据和实验支撑。 方法 将160只ICR小鼠随机分为空白对照组、低、中、高剂量组，采用随机平行对照分组法进行分组，每组各40只。实验周期为30 d，空白组灌胃生理盐水30 d，其余各组按照预设剂量灌胃复合益生菌粉30 d，灌胃结束后，活杀小鼠，观察其脏器指数、迟发性变态反应、淋巴细胞转化、血清溶血素、抗体生成细胞、自然杀伤细胞（nutral killer cells，NK）活性以及巨噬细胞吞噬功能等数据整体性评估复合益生菌粉对小鼠免疫机能的作用。 结果 相对于空白对照组，各剂量组小鼠体重和脏器指数差异无统计学意义（F = 0.885，P = 0.458；F = 0.844，P = 0.479；F = 0.732，P = 0.540）；足趾肿胀程度差值，差异有统计学意义（F = 3.252，P = 0.03）；各剂量小鼠脾淋巴细胞增殖能力光密度差值增加，差异有统计学意义（F = 48.786，P = 0.000）；溶血空斑数增加，差异有统计学意义（F = 22.095，P = 0.000）；中、高剂量组半数溶血值升高，差异有统计学意义（F = 30.544，P = 0.000）；廓清指数和吞噬指数升高，差异有统计学意义且呈剂量依赖性（F = 338.79，P = 0.000；F = 120.271，P = 0.000）；NK细胞活性同样升高，差异有统计学意义且呈剂量依赖性（F = 466.491，P = 0.000）。 结论 复合益生菌粉能提高小鼠免疫力，结果符合《保健食品检验与评价技术规范》中“具有增强免疫力功能”的评价标准。 -
关键词:
- 鼠李糖乳杆菌R9639 /
- 干酪乳杆菌Zhang /
- 植物乳杆菌P9 /
- 体液免疫 /
- 细胞免疫
Abstract:Objective To explore the effect of compound probiotic powder on enhancing related immune function of organism, so as to provide theoretical basis and experimental support for further exploration and application of compound probiotic powder in the future. Methods 160 ICR mice were randomly divided into control group, low, medium and high dose groups, and divided into groups by random parallel control grouping method, with 40 mice in each group. The experimental period lasts 30 days, the control group was given normal saline for the same time, and the other groups were given compound probiotic powder for 30 days. After the gavage, the mice were killed alive, and their organ index, delayed allergic reaction, lymphocyte transformation, serum hemolysin, antibody-producing cells, NK cell activity and macrophage phagocytosis were observed to evaluate the influence of compound probiotic powder on the immunity of mice. Results Compared with the control group, there was no significant difference in body weight and organ index of mice in each dose group （F = 0.885, P = 0.458; F = 0.844, P = 0.479; F = 0.732, P = 0.540）; The difference of toe swelling degree was statistically significant （F = 3.252, P = 0.03）.The difference of optical density of spleen lymphocyte proliferation in mice with different doses increased, and the difference was statistically significant （F = 48.786, P = 0.000）. The number of hemolytic plaque increased with statistical significance （F = 22.095, P = 0.000）. The half hemolysis value of middle and high dose groups increased, and the difference was statistically significant （F = 30.544, P = 0.000）. The clearance index and phagocytosis index increased, and the difference was statistically significant and dose-dependent （F = 338.79, P = 0.000; F = 120.271, P = 0.000）; NK cell activity also increased, and the difference was statistically significant and dose-dependent （F = 466.491, P = 0.000）. Conclusion The compound probiotic powder can improve the immunity of mice, and the results meet the evaluation standard of "having the function of enhancing immunity" in the Technical Specification for Inspection and Evaluation of Health Food. -
表 1 复合益生菌粉对小鼠体重的影响（$\bar x \pm s $，n = 10）
Table 1. Effect of compound probiotic powder on body weight of mice（$\bar x \pm s $，n = 10）
组别 初体重（g） 终重（g） 体重差值（g） 空白对照组 23.40 ± 1.01 31.06 ± 1.45 7.67 ± 1.71 低剂量组 19.74 ± 0.36 27.49 ± 1.74 7.75 ± 1.92 中剂量组 20.11 ± 0.53 26.49 ± 3.54 6.38 ± 3.41 高剂量组 20.23 ± 0.53 26.54 ± 2.59 6.31 ± 2.41 F = 0.885，P > 0.05。P为与阴性对照组比较。 
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表 2 复合益生菌粉对小鼠脏器/体重比值的影响（$\bar x \pm s $，n = 10）
Table 2. Effect of Compound Probiotic Powder on Organ/Body Weight Ratio in Mice（$\bar x \pm s $，n = 10）
组别 脾脏/体重（mg/g） 胸腺/体重（mg/g） 空白对照组 4.00 ± 0.29 2.65 ± 0.47 低剂量组 4.05 ± 0.22 2.40 ± 0.39 中剂量组 4.07 ± 0.51 2.43 ± 0.38 高剂量组 4.25 ± 0.26 2.48 ± 0.67 F = 0.844，P > 0.05；F = 0.732，P > 0.05。
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表 3 复方益生菌粉对小鼠迟发型变态反应的影响（$\bar x \pm s $，n = 10）
Table 3. Effect of Compound Probiotic Powder on Delayed Allergy in Mice（$\bar x \pm s $，n = 10）
组别 足肿胀程度差值（mm） 空白对照组 0.105 ± 0.086 低剂量组 0.117 ± 0.071* 中剂量组 0.170 ± 0.095* 高剂量组 0.215 ± 0.097* F = 3.252，*P < 0.05。
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表 4 复合益生菌粉对小鼠淋巴细胞增殖能力的影响（$\bar x \pm s $，n = 10）
Table 4. Effect of Compound Probiotic Powder on Lymphocyte Proliferation in Mice（$\bar x \pm s $，n = 10）
组别 淋巴细胞增殖能力 空白对照组 0.069 ± 0.018 低剂量组 0.095 ± 0.018* 中剂量组 0.112 ± 0.023* 高剂量组 0.174 ± 0.021* F = 48.786，*P < 0.05。
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表 5 复合益生菌粉对小鼠半数溶血值的影响（$\bar x \pm s $，n = 10）
Table 5. Effect of Compound Probiotic Powder on Half Hemolytic Value of Mice（$\bar x \pm s $，n = 10）
组别 半数溶血值（HC50） 空白对照组 68.86 ± 1.97 低剂量组 66.09 ± 2.46 中剂量组 72.74 ± 2.76* 高剂量组 77.24 ± 2.06* F = 30.544，*P < 0.05。
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表 6 复合益生菌粉对小鼠抗体细胞生成数的影响（$\bar x \pm s $，n = 10）
Table 6. Effect of compound probiotic powder on the number of antibody cells in mice（$\bar x \pm s $，n = 10）
组别 溶血空斑数（个/全脾细胞） 空白对照组 104.50 ± 18.54 低剂量组 131.38 ± 27.58** 中剂量组 165.29 ± 30.78** 高剂量组 241.67 ± 57.66** F = 22.095，P < 0.05。
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表 7 复合益生菌粉对小鼠单核-巨噬细胞碳廓清能力的作用（$\bar x \pm s $，n = 10）
Table 7. Effect of compound probiotic powder on carbon clearance of monocyte-macrophage in mice（$\bar x \pm s $，n = 10）
组别 廓清指数 吞噬指数 空白对照组 0.0049 ±0.0015 4.3025 ±0.2626 低剂量组 0.0105 ±0.0015 *5.0439 ±0.1829 *中剂量组 0.0208 ±0.0027 *6.1996 ±0.1770 *高剂量组 0.0445 ±0.0049 *7.0339 ±0.2683 *F = 338.79，*P < 0.05。
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表 8 复合益生菌粉对小鼠巨噬细胞吞噬鸡红细胞能力的影响（$\bar x \pm s $，n = 10）
Table 8. Effect of compound probiotic powder on phagocytosis of chicken red blood cells by mouse macrophages（$\bar x \pm s $，n = 10）
组别 吞噬率（%） 吞噬指数 空白对照组 0.812 ± 0.136 0.0080 ±0.0012 低剂量组 1.187 ± 0.270* 0.0118 ±0.0028 *中剂量组 1.998 ± 0.179* 0.0200 ±0.0018 *高剂量组 2.892 ± 0.402* 0.0289 ±0.0039 *F = 113.625，P = 0.000 < 0.05；F = 120.271，P = 0.000 < 0.05。
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表 9 复合益生菌粉对小鼠NK细胞活性的影响（$\bar x \pm s $，n = 10）
Table 9. Effect of compound probiotic powder on NK cell activity in mice（$\bar x \pm s $，n = 10）
组别 NK细胞活性（%） 空白对照组 28.36 ± 7.62 低剂量组 34.94 ± 4.28 中剂量组 75.92 ± 4.57* 高剂量组 114.06 ± 6.23* F = 466.491，*P < 0.05。
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