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RSS2023 Vol. 44, No. 3
2023, 44(3): 1-6.
doi: 10.12259/j.issn.2095-610X.S20230312
Abstract:
Objective To study the chemical compounds from the medicinal plants of Pleione maculata and find its bioactive compounds. Methods The compounds were extracted by alcohol （95%） and isolated by column chromatography on silica gel and Sephadex LH-20. Their structures were identified by spectroscopic analysis （1H NMR, 13CNMR and EIMS）. The antitumor activity of the compounds was studied by MTT assay in vitro. Results Ten compounds were obtained and identified as 1, 7-dihydroxy-2, 5 -dimethoxyphenanthrene （ 1 ）, 2, 7-dihydroxy-1, 5-dimethoxyphenanthrene （ 2 ）, confusarin （ 3 ）, 4, 7-dihydroxy-2-dimethoxy-9, 10-dihydrophenanthrene （ 4 ）, pleionesin B （ 5 ）, pleionesin C （ 6 ）, flavanthrin （ 7 ）, 2, 2′-dihydroxy-5, 5′, 7, 7′-tetramethoxy-9, 9′, 10, 10′-tetrahydro-3, 3′-biphenanthrene （ 8 ）, 6, 6′, 7, 7′-tetrahy- -droxy-2, 2′, 4, 4′-tetramethoxy -8, 8′-biphenanthrene （ 9 ）, 1, 3′, 5′, 7-tetrahydroxy-4, 7′ -dimethoxy-9, 9′, 10, 10′-tetrahydro-2, 2′- biphenanthrene （1 0 ）; Compounds 3, 7～10 were tested for the inhibitory activity of human lung cancer cell lines （A549）, human breast cancer drug-sensitive cell lines （MCF-7/S）, and human ovarian cancer cell lines （SKOV-3）. The results of anti-tumor activity test of compounds showed that 10 showed good inhibitory activity on three cell lines. Conclusion All compounds were isolated from this plant for the first time. Compound 10 has good inhibitory activity against three tumor cell lines, and with IC50 of 2.45, 6.83, 4.23 μM, respectively.
2023, 44(3): 7-14.
doi: 10.12259/j.issn.2095-610X.S20230322
Abstract:
Objective To find out the more stable rat models of intrauterine adhesions, so as to provide the basis for further investigation into the mechanism of intrauterine adhesions occurrence and exploration of novel treatments by constructing rat models of intrauterine adhesions with different measures. Methods Forty-eight female SD rats in estrus were randomly assigned to one of the four groups: control group, lipopolysaccharide cotton thread group （with line group）, lipopolysaccharide injection group （injection group）, lipopolysaccharide gelatin group （gelatin group）, each group consisted of 12 rats. Rats in the gelatin group placed a gelatin sponge strip soaked in lipopolysaccharides in the uterine cavity after curettage. In each group, 4 rats were sacrificed 7 days, 14 days, and 28 days later, respectively, and endometrial tissue was detected, the number of endometrial glands and the area of fibrosis of the rats were observed by HE staining and Masson staining and the expression of the fibrotic protein TGF-β1 was detected by immunohistochemistry. Western Blot was used to detect the protein expression of fibrotic proteins Collagen-I, Collagen-III, α-SMA, and TGF-β1. Result （1） The results of HE staining showed that the number of glands in each group decreased compared with the control group after 7 days, 14 days, and 28 days of modeling （P < 0.05）. Of these, after 14 days and 28 days of modeling, the number of glands in the injection group decreased more significantly than those in the line and gelatin e group （P < 0.05）, and when modeling for 28 days the number of glands in the injection group was close to zero. （2） Masson staining showed that the degree of fibrosis of the endometrium in the lineage-based group, injection group, and gelatin group was significantly higher than that of the control group at 7 days, 14 days, and 28 days （P < 0.05）, and after comparison between different groups, it was found that the degree of endometrial fibrosis of rats in the injection group was higher than that in the line-and-line group and gelatin group （P < 0.05）, and the injection group after 28 days of molding was the most severe group. （3） Immunohistochemical staining showed that TGF-β1 expression in the lineage group, there was a significant increase in the injection group and gelatin group compared to the control group at 7 days, 14 days, and 28 days of modeling, and the differences in the other groups were statistically significant with the exception of the line and gelatin group for 7 days of molding （P < 0.05）. Furthermore, at 7 days and 28 days after modeling, TGF-β1 expression in the injection group was greater than that in the gelatin group （P < 0.05）. （4） Western blot analysis showed that the expression of fibrosis related proteins was up-regulated by different methods of infective bacterial lipopolysaccharide treatment after mechanical injury, and upon a comparison between different groups, it was found that the expression of Collagen-I, Collagen-III, α-SMA, and TGF-β1 in the injection group was increased in comparison to that in the gel-treated group （P < 0.05）, but the difference between the gelatin e group and the strip group did not reach statistical significance （P > 0.05）. After 7 days of molding, the expression Collagen-I, Collagen-III And α-SMA in the injection group was higher （P < 0.05）, 14 days after molding, the expression of Collagen-III andα-SMA （P < 0.05） in the injection group was higher than that in the line group, and the expression of Collagen-I, Collagen-III, α-SMA, and TGF-β1 in the injection group was greater （P < 0.05）. The expression of Collagen-I, Collagen-III, α-SMA and TGF-β1 protein in the control group, the line group, the injection group, and the gelatin group was the highest, and after mechanical injury, the effect of lipopolysaccharide injection to damage the uteri of rats was the most significant. Conclusions The rat model of intrauterine adhesions obtained by intrauterine injection of lipopolysaccharide after curettage is more severe than that obtained by intrauterine placement of a cotton string of lipopolysaccharide or lipopolysaccharide gelatin and the rat model of intrauterine adhesions has the most severe damage 28 days after modeling. The placement of lipopolysaccharide gelatin in the uterine cavity following curettage is a novel method that may also result in intrauterine adhesions.
2023, 44(3): 15-21.
doi: 10.12259/j.issn.2095-610X.S20230321
Abstract:
Objective To establish a method for the determination of R-（+） -propranolol and S-（-） -propranolol in the incubation system of liver microsomes by HPLC-MS/MS, and to compare the metabolic characteristics of R-（+） -propranolol and S-（-） -propranolol in rat, dog, monkey and human liver microsomes. Methods R-（+）-propranolol and S-（-）-propranolol were respectively dissolved in NADPH activated liver microsome incubation systems of different species, and acetonitrile was added to terminate the reaction after incubation for different time. The concentrations of R-（+） -propranolol and S-（-） -propranolol in each incubation system were determined by using an electrospray ion source（ESI） with carvedilol as the internal standard and positive ion scanning under multi-reaction monitoring mode（MRM）. The residual percentage, metabolism half-life period in vitro and intrinsic clearance of R-（+） -propranolol and S-（-） -propranolol in incubation systems of different species liver microsomes were calculated based on the mass concentration of propranolol with different configurations at 0 min. Results The linear range of propranolol: 0.05～10.00 μg/mL; and the lower limit of quantitation: 0.05 μg/mL; The RSD% of intra-day and inter-day were less than 13%. In the incubation system of four species liver microsomes, the metabolism of R-（+）-propranolol in rat liver microsomes was fast, followed by monkey liver microsomes, and was slow in dog and human liver microsomes, and the t1/2 values in vitro were as follows: rat < monkey < dog < human. S-（-）-propranolol was metabolized quickly in rat and dog liver microsomes, but slowly in monkey and human liver microsomes, and the t1/2 values in vitro were as follows: rat < dog < monkey < human. In rat, dog and monkey liver microsomes, the t1/2 of R configuration is greater than S configuration, and CLint is smaller than S configuration, but in human liver microsomes, the opposite is true. Using univariate analysis of variance, the in vitro metabolic half-life t1/2 and intrinsic clearance CLint of R -（+） - propranolol and S -（-） - propranolol in four liver microsomes were significantly different（P < 0.05）. Conclusion The established HPLC-MS/MS method is simple, rapid and accurate, and can be used for the determination of propranolol enantiomer mass concentration in liver microsome. The metabolic stability of propranolol with different configurations in rat, dog, monkey and human liver microsomes was significant different.
2023, 44(3): 22-27.
doi: 10.12259/j.issn.2095-610X.S20230313
Abstract:
Objective To explore the regulatory role of PI3K/Akt signaling pathway in the hypertrophic scar. Methods The rabbit ear hypertrophic scar model was established and randomly divided into four groups, including normal rabbit ear skin group （A）, hypertrophic scar model group （B）, DMSO-stimulated hypertrophic scar model group （C）, and PI3K-specific inhibitor （LY294002） stimulated model group （D）. Morphological changes of rabbit ear scar were observed by histopathology. The expression of phosphorylation of Akt[S473] and Akt[T308] was examined by immunohistochemistry and western blot. Immunofluorescence double staining was performed to assess the co-localization of p-Akt[S473] and p-Akt[T308]. The TUNEL assay was used to detect the fibroblasts’ apoptosis in skin tissues. Results Scar thickness and fibroblast count were significantly higher in the D group than that in the B and C groups, but the difference was not statistically significant between the B group and the C group. The results of immunohistochemistry and western blot assay confirmed that the expression of p-Akt[T308] and p-Akt[S473] were significantly higher in the B group than in the A group, but significantly lower in the D group than in the C group. The expression of p-Akt[T308] and p-Akt[S473] was significantly enhanced in the B group compared with the C group. Immunofluorescence double staining showed that p-Akt[T308] and p-Akt[S473] were mainly co-localized in the cytoplasm in scar tissue, and their co-expression was lower in the D group than in the B group. TUNEL assay results confirmed that the apoptosis level of fibroblast was significantly downregulated in skin tissue in the B group compared with the A group, and the apoptosis level of fibroblast was higher in the D group than in the C （P < 0.01）. Conclusion Blocking the PI3K/Akt signaling pathway can accelerate fibroblast apoptosis by inhibiting Akt phosphorylation, and then alleviate hypertrophic scar formation.
2023, 44(3): 28-33.
doi: 10.12259/j.issn.2095-610X.S20230320
Abstract:
Objective To investigate the effect of Twist1 on invasion and metastasis of gallbladder carcinoma cells and the regulation of Bmi1, E-Cadherine andN-Cadherine. Methods Twist1 shRNA lentivirus vector was constructed to infect GBC-SD cells as the experimental group. The empty lentivirus-infected GBC-SD cells were the negative group, and the untreated GBC -SD cells were the blank group. Cell migration and invasion ability were detected by cell scratch and Transwell chamber invasion assay. Western blot and RT-PCR were used to detect the relative expression levels of Twist1, Bmi1, E-Cadherine and N-Cadherine. Results Compared with the negative and blank groups, the experimental group had a lower cell scratch healing degree and fewer invasion numbers （P < 0.0001）. There was no significant difference in cell migration rate and invasion number between the two control groups （P > 0.05）. The relative expression levels of Twist1, Bmi1 and N-Cadherine in the negative group and blank group were obviously higher than those in the experimental group （P < 0.001）. The relative expression levels of E-Cadherine protein and mRNA were obviously lower than those of the experimental group （P < 0.0001）. There was no significant difference in the relative expression levels of protein and mRNA between the negative group and the blank group （P > 0.05）. Conclusion Targeted silencing of Twist1 can inhibit the migration and invasion of gallbladder carcinoma cells, and the mechanism may be related to inhibiting the expression of Bmi1 and the process of epithelial-mesenchymal transformation.
2023, 44(3): 34-43.
doi: 10.12259/j.issn.2095-610X.S20230305
Abstract:
Objective To explore the effects of MMP-1 and MMP-7 on pulmonary arterial hypertension （PAH）. Methods The contents of MMPs （MMP-1, MMP-2, MMP-7, MMP-9, MMP-10, MMP-14） in the serum of PAH patients and healthy controls were detected and two MMPs were selected for in vivo experiments. Clinical information was collected and body mass index （BMI）, NYHA cardiac function class, 6 min walking distance （6MWD）, brain natriuretic peptide （BNP）, mean pulmonary arterial pressure （mPAP）, triglyceride （TGs）, low-density lipoprotein （LDL）, total cholesterol （TC）, hemoglobin （HB）, heart rate （HR） were compared between PAH patients and healthy controls. The PAH rat model was induced by monocrotaline, and then MMP-1 overexpression plasmid and shMMP-7 plasmid were used to up-regulate and down-regulate the expression of MMP-1 and MMP-7, respectively. Hematoxylin-eosin （HE） staining was used to observe the pathological of PAH, Masson staining was used to observe the changes of muscle fibers and collagen fibers, and immunofluorescence was used to detect the expression of α-SMA and VWF. The left ventricle, right ventricle and interventricular septum were weighed and the right ventricular hypertrophy index was calculated. Results The serum levels of MMP-1 （P < 0.0001）, MMP-2 （P < 0.001）, MMP-7 （P < 0.01）, MMP-9 （P < 0.001）, MMP-10 （P < 0.001） and MMP-14 （P < 0.01） in PAH patients were significantly higher than those in healthy controls. 6MWD was significantly decreased （P < 0.05）, BNP level was increased （P < 0.001）, NYHA class was increased （P < 0.0001）. Compared with the control group, the protein expression of MMP-1 （P < 0.0001） and MMP-7 （P < 0.0001） in lung tissue, right ventricular hypertrophy index （P < 0.0001）, pulmonary artery vascular thickness （P < 0.05）, and collagen fiber deposition （P < 0.05） were increased in PAH rats. The expressions of α-SMA （P < 0.05） and VWF （P < 0.05） in blood vessels were increased in PAH rats than control rats. Compared with PAH group, overexpression of MMP-1 in PAH rats promoted the development of PAH, while interference with MMP-7 delayed its development. Conclusions The serum levels of MMPs （MMP-1, MMP-2, MMP-7, MMP-9, MMP-10, MMP-14） are increased in patients with PAH. MMP-1 and MMP-7 promote pulmonary artery remodeling, pulmonary fibrosis and right ventricular dysfunction in PAH rats.
2023, 44(3): 44-48.
doi: 10.12259/j.issn.2095-610X.S20230324
Abstract:
Objective By taking the “Guidelines for the Prevention and Treatment of Hypertension in China” （2022 revised edition） as the gold standard, and “Clinical Practice Guidelines for Hypertension in China” （November 2022 edition） as the test standard, to compare and analyze the epidemic trend and distribution characteristics of hypertension in 18-year-old students in Yunnan Province, and evaluate the scientifically and rationality of the new guidelines for diagnosing hypertension in 18-year-old students in Yunnan Province were explored, so as to improve the scientific basis for formulating the criteria for determining abnormal blood pressure in students in the future. Methods The survey of blood pressure among 18-year-old students investigated in 16 prefectures （cities）, 32 counties （districts） and 61 schools in Yunnan in 2021 were used Kappa for comparative analysis. A total of 3175 people were surveyed in this study. Results The diagnostic assessment of the new guideline is ≥130 mmHg/80 mmHg, which is lower than the gold standard （≥140 mmHg/90 mmHg）. To diagnose with the gold standard, t he prevalence of hypertension among 18-year-old students in Yunnan was 2.93%. The diagnostic criteria of the New Guidelines were 16.13%. The diagnostic value of the new guideline with hypertension was significantly lower than the gold standard, resulting in the prevalence level being significantly higher than the gold standard （χ2 = 498.31, P < 0.01）. The Kappa value was 0.27 （P < 0.01）, which indicated weak consistency with diagnosing in the New Guidelines. The authenticity evaluation was as follows: sensitivity 100%, specificity 86.40%, misdiagnosis rate 13.60%, Jordon index 0.86, positive likelihood ratio 7.35. The reliability evaluation: the compliance rate was 86.80%. Conclusions The diagnostic criteria of the New Guidelines have been lowered. Taking into account the threshold of hypertension prevention and treatment, it is moved forward. However, evidence to downgrade diagnostic criteria may not be sufficient and caution is advised.
2023, 44(3): 49-53.
doi: 10.12259/j.issn.2095-610X.S20230308
Abstract:
Objective To establish TMJ-OA rat models by occlusal disorder and forced open mouth, compare two TMJ-OA induction methods and assess their applicability by pathological changes in the cartilage, subchondral bone. Methods Twenty-one 8-week-old Sprague-Dawley （SD） male rats were divided into four groups, namely control group, and three rats were conventional feeding. eighteen SD rats were randomly allocated to three groups using the randomization table; each group had the same number of SD rats. The Forced open mouth （OP） , steady mouth opening was imposed to 20 mm daily （1 minute/day for 16 days）. the occlusal disorder group （OD）, an orthodontic ligation silk （0.25 mm diameter） revolved around the first molar, and a ligation knot was created on the first molar of the maxillary. The control group of occlusal disorder （ODS） use the same method, a ligation knot in the mesial of the first molar. Rat models were employed and followed for 4 weeks after experimental procedures, and TMJ joints in each group were harvested. The TMJ changes were evaluated by micro-CT, HE staining, and Safranin-O/Fast green staining. Results We found apparent histological phenotypes of TMJ-OA in the OP and OD groups, and observed a pronounced drop in bone volume fraction , BV/TV and Tb.Th between OD and OP groups had no significant difference （P > 0.05）, and a substantial increase in the modified Mankin score was found, there was no significant difference in the two groups （P > 0.05）, but two samples of OP group didn’t present apparent histological phenotypes of TMJ-OA. Conclusions Osteoarthritis-like pathological changes in the cartilage and subchondral bone can be observed in both of the two methods. The occlusal disorder is a stable TMJ-OA model, forced open mouth caused osteoarthritis-like changes, but it’s unstable and inefficient.
2023, 44(3): 54-60.
doi: 10.12259/j.issn.2095-610X.S20230306
Abstract:
Objective To investigate the clinicopathological characteristics of mucinous breast carcinoma （MuBC） and its relationship with prognosis, so as to provide reference for patients to make a personalized treatment plan. Methods The clinical and pathological data of 139 MuBC patients admitted to Yunnan Cancer Hospital from January 2008 to September 2021 were retrospectively collected. They were divided into pure mucinous breast carcinoma （PMuBC） group （n = 76） and mixed mucinous breast carcinoma （MMuBC） group （n = 63）, and compared between two groups. COX proportional hazards regression model was used to determine the prognostic factors of MuBC.Kaplan-Meier and log-rank tests were used for survival analysis. Results The 10-year overall survival rate of PMuBC group was higher than that of MMuBC group （98.6% vs 83.7%, P = 0.019）. The proportion of uninvolved lymph nodes （81.6% vs 47.6%, P < 0.001） in PMuBC group was higher than those in MMuBC group.COX multivariate analysis showed that tumor size was stage T1 （P = 0.005）, tumor size was stage T2 （P = 0.006） and positive PR （P = 0.033） were prognostic protective factors. Lymph node involvement reached stage N3 （P = 0.052） and distant metastasis （P = 0.025） are prognostic risk factors. Conclusions Tumor size, lymph node metastasis, distant metastasis and PR status are independent prognostic factors for MuBC. Compared with MMuBC, PMuBC has lower lymph node metastasis rate, higher positive rate of PR and better prognosis.
2023, 44(3): 61-67.
doi: 10.12259/j.issn.2095-610X.S20230309
Abstract:
Objective To explore the bacterial distribution and drug resistance of sterile body fluid specimens （excluding blood） in nine tertiary general hospitals in Yunnan Province from 2017-2021, and to provide a reference basis for the rational clinical use of antibiotics in the region. Methods Repeated strains from the same patients were excluded, and drug sensitivity tests were performed by instrumental method or paper method, and the results were interpreted according to the 2021 CLSI standard. Results Ascites was the specimen type with the highest strain isolation rate, accounting for 32.7%. A total of 5305 strains were isolated from all specimens, with Gram-positive bacteria accounting for 53.3%, predominantly Staphylococcus epidermidis, and Gram-negative bacteria accounting for 46.7%, predominantly Escherichia coli. Methicillin-resistant coagulase-negative staphylococci and methicillin-resistant Staphylococcus aureus were comparable in both cases and should not be underestimated. Enterococcus faecalis showed a decreasing trend of resistance to commonly used antimicrobials. The resistance rates of Klebsiella pneumoniae to meropenem and imipenem were 35.6% and 40.3%, respectively, both of which were higher than the 2021 CHINET level, and the resistance rates showed a trend of increasing year by year. Acinetobacter baumannii was highly resistant to most antibiotics, especially to carbapenems with a resistance rate of 82%. Conclusion The source of pathogenic bacteria in sterile body fluid is extensive, and the form of carbapenem resistance among different genera is severe. It is still necessary to focus on monitoring the third-generation cephalosporin-resistant Enterobacteriaceae and carbapenem-resistant Enterobacteriaceae and Acinetobacter baumannii.
2023, 44(3): 68-73.
doi: 10.12259/j.issn.2095-610X.S20230303
Abstract:
Objective To understand the prevalence of chronic diseases among the rural elderly in Yulong County and Fumin County of Yunnan Province, explore and analyze the relationship between elderly chronic diseases and physical labor intensity, and provide targeted prevention and control strategies for the prevention and treatment of elderly chronic diseases. Methods Multi-stage stratified random sampling method was used in this survey and the elderly 2299 elderly people aged ≥60 years in rural areas of Yulong County and Fumin County of Yunnan Province were selected. The elderly aged 60 years and above were investigated with questionnaires and physical examinations on the prevalence of 10 chronic diseases, including diabetes, hypertension, coronary heart disease, stroke, COPD, asthma, hyperlipidemia, chronic kidney disease, gastrointestinal ulcer, and cataract. Descriptive analysis, χ2 test and multivariate logistic regression analysis were used to analyze the influencing factors. Results There were statistically significant differences between different ethnic groups in whether they have read books, whether they live alone, whether they are farmers, whether they are physical labor intensity, and whether they are obese （χ2 test, P < 0.05）. The prevalence of chronic diseases in the elderly was 83.3%, of which the prevalence of chronic diseases in the Han nationality was 83.1%, and the prevalence of chronic diseases in the Naxi nationality was 83.6%. With the increase of age, in addition to the decline in the prevalence of chronic diseases in the population aged 75 and over, the prevalence of chronic diseases in the elderly population in the surveyed areas showed an upward trend, and the difference was statistically significant （χ2 test, P < 0.05）. Central obesity was a risk factor for chronic diseases in the elderly. The risk of chronic diseases in elderly people with central obesity was 1.895 times that without central obesity. The moderate-intensity physical labor was a protective factor for elderly chronic diseases. The risk of chronic disease of moderate-intensity physical labor was 0.643 times that of low-intensity physical labor, and the difference was statistically significant （P < 0.05）. With the increase of age, the proportion of high-intensity physical labor showed a downward trend, and the proportion of light-intensity physical labor showed an upward trend, and the difference was statistically significant （χ2 test, P < 0.05）. Conclusions Central obesity and physical labor intensity are the influencing factors of chronic diseases in the elderly. There are differences in physical labor intensity with the increase of age, suggesting that to prevent chronic diseases in the elderly, it is necessary to continue to do a good job in health education for the elderly in terms of lifestyle and to further prevent the aging occurrence of chronic diseases.
2023, 44(3): 74-80.
doi: 10.12259/j.issn.2095-610X.S20230314
Abstract:
Objective To compare the operation time, acceptability and sealing effect of resin-based sealant and resin-reinforced glass ionomer combined with certain adhesive systems applied to seal deciduous molar teeth, so as to provide a more appropriate oral health promotion activity for children in areas with insufficient oral medical resources. Methods The qualified second deciduous molars were randomly divided into three groups: ClinproTM Sealant + phosphoric acid etching （the control group）, ClinproTM Sealant + Single bond Universal, ClinproTM XT Vanish + Single bond Universal.The pit and fissure sealant were applied, operation time and treatment sensitivity were recorded. After 3, 6, and 12 months, preservation conditions and caries incidence were reviewed. Results （1）A total of 62 people in SO group included 228 teeth, 63 people in SS group included 234 teeth, and 56 people in VS group included 221 teeth. （2）The operating time of SE group （181.88±12.37） s and SS group （103.07±11.37） s was significantly different from that of SO group （108.56±8.65） in control group （Z = 460.163, P < 0.001）. （3） Compared with the other two groups, the treatment sensitivity of SO group was worse （Z = 20.232, P < 0.001）. （4） At reexamination 3 months after operation, the preservation rate of sealant and the incidence rate of caries were 100% and 0% in SO group, 62.1% and 2.2% in SS group, and 93.1% and 1.8% in VS group, respectively; At reexamination 6 months after operation, the preservation rate of sealant and incidence rate of caries in SO group were 98.2% and 1.8% respectively, the preservation rate and incidence rate of caries in SS group were 52.3% and 4.2%, and the preservation rate and incidence rate of caries in VS group were 89.2% and 3.3% respectively; At reexamination 12 months after operation, the preservation rate of sealant and the incidence rate of caries in SO group were 95.0% and 5.0% respectively; the preservation rate and the incidence rate of caries in SS group were 42.8% and 7.2%, and the preservation rate and the incidence rate of caries in VS group were 78.9% and 6.2%. Among the three reexaminations, the preservation rate of SO group was the highest, and the difference in the preservation of sealants among the three groups was statistically significant （ χ2 = 144.286, P < 0.001; χ2 = 159.703, P = < 0.001; χ2 = 152.945, P < 0.001）, but the caries prevention effect of the three groups was similar （ χ2 = 24.842, P = 0.089;χ2 = 2.153, P = 0.341; χ2 = 0.915, P = 0.613） Conclusion The self-etching adhesive combined with resin-reinforced glass ionomer can simplify the operation procedure, improve the comfort of children, and achieve good sealing effect, which is a practical and effective choice for the promotion of sealing technology in economically underdeveloped areas.
Analysis of HPV Detection in 18 449 Hospital Patients in Kunming and HPV Vaccination Recommendations
2023, 44(3): 81-86.
doi: 10.12259/j.issn.2095-610X.S20230302
Abstract:
Objective To analyze the detection and subtype distribution characteristics of human papillomavirus （HPV） in the genital tract of the first outpatient in the hospital, and to provide reference for the prevention and vaccination of HPV in the region. Methods A total of 18449 first-diagnosed female patients in Yan 'an Hospital of Kunming City from January 2018 to December 2020 were selected as the research objects. Clinical data were collected and a retrospective study was used to analyze the total HPV infection and compare the HPV detection rates in different age groups and years. To analyze the prevalence of high-risk HPV （HR-HPV） and low-risk HPV （IR-HPV）. Results Among 18449 patients, 2520 were HPV positive, and the total detection rate of HPV was 13.7%. HPV infection showed a downward trend from 2018 to 2020 （trend χ2 = 4.95, P < 0.001）. According to age group, HPV detection showed a "V" type distribution, with the highest detection rate of 42.4% in the group less than 20 years old. The second group was ≥60 years old （19.7%）. The detection rate in 30-year old group was the lowest, only 12.0%, and the difference was statistically significant （ χ2 = 7.080, P < 0.001）. The minimum age of infection was only 15 years and there were multiple infections. There were 973 cases of multiple infection, accounting for 5.3% of all patients and 38.6% of positive patients. Multiple infection was the main infection in < 20 years old group and ≥60 years old group, and single high-risk infection was the most common infection in 20-59 years old group. The top five detected HR-HPV types were HPV52, 53, 58, 16 and 51. The IR-HPV types were HPV81, 42, 43, 6 and 11. In HR-HPV, the dominant detected types changed from HPV52, 16 and 58 in 2018 to HPV52, 53 and 58 in 2019 and 2020. Conclusions HPV infection shows a trend of younger age, and the dominant types of HPV infection are different in different age groups. Attention should be paid to the prevention and treatment of HPV52, 53 and 58 infections in this region.
2023, 44(3): 87-91.
doi: 10.12259/j.issn.2095-610X.S20230325
Abstract:
Objective To investigate the clinical significance and monitoring value of fibrin monomer （FM）after surgery. Methods A total of 93 patients after surgery admitted to the Department of Critical Care Medicine, the Second Affiliated Hospital of Kunming Medical University from December 2021 to May 2022 were retrospectively analyzed. Plasma fibrin monomer （FM）, antithrombinⅢ （AT-Ⅲ）, von willebrand factor （vWF） and fibrin degradation products （DD ） were collected before operation （T0）, on the day after operation （T1） and 24 hours after operation （T2）. According to the normal reference range of FM value, patients were divided into normal group, low level group, medium level group and high level group. The clinical data of patients were statistically analyzed, the influencing factors of FM were analyzed by ordinal logistic regression, the correlation between vWF, AT-Ⅲ, DD dimer and FM was analyzed by multivariate regression, and the correlation between Fib and FM was analyzed by univariate regression. Results （1） A total of 93 patients were enrolled, with an average age of 59±13 years, including 49 males and 44 females. Of these, 61 were malignant （66%） and 32 were non-tumor （34%）. （2） The patients were divided into normal group （≤5 μg/mL, n = 9 （9.7%）, FM （4.32±1.07） μg/mL. The low level group was 6-54 μg/mL, 39 cases （41.9%）, FM （20.67±13.25） μg/mL; Medium level group （55～103 μg/mL, 11 cases （11.8%）, FM （73.96±13.38） μg/mL; High level group ≥104 μg/mL, 34 cases （36.6%）, FM （172.30±26.78） μg/mL.Analysis of variance （ANOVA） showed that the difference between the normal level group and the low level group was statistically significant （P < 0.05）, and the difference between the other groups was significant （P < 0.0010.00）. Ordinal logistic regression analysis showed that blood loss volume, vWF, primary disease, age and gender had no significant effect on fibrin monomer （FM） （P > 0.05）. （3） Bivariate correlation analysis showed that FM was not correlated with Fib at T0 and T1, but was negatively correlated with Fib at T2 （r = -0.258, P < 0.05）. DD at T2 was positively correlated with FM （r = 0.536, P = 0.000）. （4） The plasma fibrin monomer （FM） concentration of patients with liver malignant tumor after surgery （76.4 μg/mL） was significantly higher than that of patients with gastrointestinal tumor after surgery （25.7 μg/mL）. DunnettT3 test showed that the difference was statistically significant （P < 0.05）. Conclusions Monitoring of thrombin activation marker fibrin monomer （FM） in patients after surgery has certain clinical significance, which is expected to be an early marker for predicting thrombosis or guiding anticoagulant therapy. A large amount of thrombin activation after surgery can consume fibrinogen （Fib）, and attention should be paid to the concentration of fibrinogen to avoid postoperative bleeding.
2023, 44(3): 92-96.
doi: 10.12259/j.issn.2095-610X.S20230315
Abstract:
Objective To investigate the feasibility of measuring the thickness of adult subpatellar fat pad based on MRI and the correlation between the thickness of adult subpatellar fat pad and sex, age, weight and height, to provide the basis for keeping the thickness of subpatellar fat pad in total knee arthroplasty. Methods 1019 cases of 18-80-year-old adult knee MRI scan images were randomly selected as the research object, and the median sagittal image of the knee joint was taken as the measurement plane. The distance of the farthest point of the edge was used as the measurement thickness, the measurement results were statistically analyzed by SPSS26.0 statistical software package, the measurement data of normal distribution were expressed by \begin{document}$\bar x \pm s $\end{document} ![]()
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Results The average thickness of the infrapatellar fat pad in males was greater than that in females, which were （28.08±4.64） mm and （24.58±4.22） mm, respectively, and the difference was statistically significant （P < 0.05）. The ages of males and females were （49.09±17.78） years old, respectively. and （50.42±17.88） years old, the difference was not statistically significant （P > 0.05）; the height and weight of males were greater than those of females, and the difference was statistically significant （P < 0.05）, which was considered as a physiological difference; male infrapatellar fat pad There was no correlation between the thickness and age, height and weight （P > 0.05）; the thickness of the infrapatellar fat pad in women was correlated with body weight （P < 0.05）, but not with age and height （P > 0.05）. Conclusion The average thickness of subpatellar fat pad in male is larger than that in female and has no correlation with age, height and weight.
2023, 44(3): 97-102.
doi: 10.12259/j.issn.2095-610X.S20230328
Abstract:
Objective To investigate the potential of cardiac myosin binding protein-C （cMyBP-C） as a novel marker of myocardial infarction by detecting the concentration of cardiac myosin binding protein-C （cMyBP-C） in venous blood of patients with acute myocardial infarction. Methods A total of 120 patients with chest pain as the first symptom and within 24 hours of the onset of chest pain were collected and divided into three groups, including 40 patients with acute myocardial infarction （group A）, 20 patients with angina pectoris （group B）, and 60 patients with non-cardiogenic chest pain （Group C）; 30 healthy volunteers （Group D） were selected as the control group. All patients with chest pain were immediately drawn elbow venous blood after admission, and 3 hours after admission. The control group was immediately drawn venous blood once after admission. The concentrations of cMyBP-C and cTnI in each sample were measured by ELISA and chemiluminescence. Results （1） The concentrations of cMyBP-C and cTnI in group A were significantly higher than those in groups B, C and D （P < 0.05）; （2） In group A, the onset time of 9 patients was less than 4h. The concentration of cMyBP-C measured by blood sampling immediately after admission was significantly higher than that in Group D. the concentration of cTnI measured by blood sampling immediately after admission did not increase significantly, and the difference was statistically significant （P < 0.05）; In group A, 35 patients underwent emergency PCI immediately after admission. The concentration of cMyBP-C measured after operation （3h after admission） was significantly lower than that before operation, and the concentration of cTnI after operation was higher than that before operation. The difference was statistically significant （P < 0.05）; （3） There was a positive correlation between cMyBP-C and cTnI concentrations in groups a, B and C. the AUC value of ROC curve of cMyBP-C in diagnosing acute myocardial infarction with onset time less than 24 hours was 0.954, the sensitivity was 92.5%, and the specificity was 90.0%. Conclusion cMyBP-C, similar to cTnI, has the potential to be used as a biomarker for early diagnosis of myocardial infarction and as an early evaluation index for the condition and surgical effect after PCI.
2023, 44(3): 103-112.
doi: 10.12259/j.issn.2095-610X.S20230316
Abstract:
Objective To explore the difference between duodenum-preserving pancreatic head resection （DPPHR） and pancreatic-preserving pancreatic duct jejunostomy （Partington operation） in the treatment of pancreatic head calculus, and evaluate the advantages and disadvantages of the two operations, and optimize and improve them. Methods From January 1, 2017 to October 1, 2022, the clinical, imaging and pathological data of 139 patients with pancreatic head stones requiring surgical treatment admitted to the Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital of Kunming Medical University were retrospectively analyzed. According to the different surgical methods, the patients were divided into DPPHR group and Partington group. The clinical characteristics of patients in the two groups and the short-term and long-term outcomes of surgical treatment were analyzed. Results There was no significant difference in preoperative general data （P > 0.05）. Type Ⅰ stones were predominant in DPPHR group （80.2%）, while type Ⅰ and Ⅲ stones were predominant in Partington group （45.3%, 47.2%）, and the difference was statistically significant （ P < 0.001）. In terms of short-term efficacy, DPPHR group had advantages over Partington group in terms of operation time, intraoperative blood loss, postoperative hospital stay and postoperative complication rate （ P < 0.05）. In the long-term efficacy, the DPPHR group was better than the Partington group in stone recurrence reoperation and postoperative pancreatic function （ P < 0.05）. Logistic multivariate analysis showed that CP≥5 a （95%CI 1.057-3.884, P = 0.012） and long postoperative drinking history （95%CI 0.987-3.128, P = 0.025）. CP≥5 a and a long history of postoperative alcohol consumption were independent risk factors for survival without pain recurrence after pancreatic head calculi surgery Conclusions Based on the analysis of the short-term efficacy （operation time, intraoperative blood loss, postoperative hospital stay and postoperative complication rate） and long-term efficacy （stone recurrence and reoperation, postoperative pancreatic function） of patients with pancreatic head calculi in this study, DPPHR surgery was superior to Partington surgery, suggesting that DPPHR surgery has become an effective treatment for PDS at present. However, it is also necessary to develop an individualized surgical strategy for the patient, with emphasis on postoperative care and follow-up to provide the best outcome for the patient.
2023, 44(3): 113-119.
doi: 10.12259/j.issn.2095-610X.S20230327
Abstract:
Objective To observe the effect of combined guidance of optical coherence tomographic （OCT） and quantitative flow ratio （QFR） in patients with In-stent restenosis （ISR） of intermediate severity. Methods Patients with intermediate ISR lesions were performed PCI or given OMT under the guidance combined OCT imaging and QFR. PCI was performed if QFR was ≤0.80 or QFR > 0.8 but OCT showed minimal luminal area < 2.0 mm2 and the in-stent plaque was unstable and more apt to rupture. Patients with QFR > 0.8 and negative OCT results were given optimal medical therapies, and major adverse cardiac events were assessed at the end of follow-up. The pre-defined primary endpoint was the composite of major adverse cardiac events or recurrent angina at one year. Results A total of 48 patients （with 57 intermediate ISR lesions ） were enrolled. There are 29 lesions of QFR （-）OCT （-）; 16 lesions of QFR （+）OCT （+）, 8 lesions of QFR （-）OCT （+） and 4 lesions of QFR （+）OCT （-）. We found low occurrence （5.3%） of the primary endpoint of major adverse cardiac events or recurrent angina after one year follow-up.No significant difference was found in the incidence of major adverse cardiac events between the 4 groups. Conclusions In patients with intermediate ISR lesions, Combined OCT and QFR Guidance can provide useful prognostic information, reduce the number of unnecessary PCI Procedures and is associated with a lower occurrence of the composite of major adverse cardiac events
2023, 44(3): 120-124.
doi: 10.12259/j.issn.2095-610X.S20230304
Abstract:
Objective To explore the effects of childhood abuse on Non-suicidal Self-injury（NSSI） in patients with first-episode adolescent depression in the Yunnan Provincial Psychiatric Hospital. Methods A total of 136 depressed patients aged 12 to 18 years hospitalized in our hospital from January to August 2022 were included, and the general demographic questionnaire, Adolescent non-suicidal self-injury behavior questionnaire, and Childhood Trauma Questionnaire were used. The patients were divided into NSSI group and non-NSSI group according to the presence or absence of NSSI behavior in the past and the investigation results were compared between the two groups. Multivariate logistic regression analysis was used to analyze the impact of childhood maltreatment on NSSI. Results Compared with the non-NSSI group, the incidence of emotional abuse and emotional neglect in the NSSI group were higher, and the differences were significant （P < 0.05）. After adjusting for gender, education, parental divorce, and family history of mental illness, emotional abuse and emotional neglect were significantly positively associated with NSSI （OR = 3.015, 95%CI: 1.200～7.578; OR = 2.446, 95%CI: 1.089～5.496）. Conclusion Emotional abuse, emotional neglect are strongly associated with NSSI.
2023, 44(3): 125-130.
doi: 10.12259/j.issn.2095-610X.S20230317
Abstract:
Objective To observe the clinical efficacy and pregnancy outcome of hysteroscopic cold knife system in patients with moderate and severe intrauterine adhesions who planned to undergo assisted reproduction. Methods Patients with moderate and severe intrauterine adhesions who planned to undergo assisted reproduction were retrospectively analyzed and divided into cold knife group and electric energy group according to different surgical methods. The basic data and surgical data of all patients were collected, and the pregnancy outcomes of patients were followed up. Results （1） A total of 120 patients were collected in this experiment, including 60 patients in cold knife group and 60 patients in electric energy group. There were no significant differences in operation time, intraoperative blood loss, intraoperative complications, postoperative pregnancy rate and live birth rate between the two groups （P > 0.05）. In the cold knife group, the proportion of the uterine cavity shape and endometrium restored to the condition of transplantation after two operations was 63.3%; Electrical energy group accounted for 43.3%. The median gestational month of the cold knife group was 2.5 months, which was 1 month earlier than that of the electric energy group. （2） To the extent to which caused by intrauterine adhesions, cold knife group, patients with severe caused by intrauterine adhesions in operative time and intraoperative blood loss and postoperative pregnancy rate and live-birth rate no statistical difference （ P > 0.05） electrical energy group of uterine cavity adhesion of surgery in patients with severe bleeding amount of time and have more moderate caused by intrauterine adhesions, and statistically significant （ P < 0.005）. In the electrical energy group, only 7.7% of the patients with severe adhesions who achieved transplantation conditions after two operations were the lowest among all groups. Conclusions For the patients with moderate and severe intrauterine adhesions who need embryo transfer, the hysteroscopic cold knife system is safe and effective, and the number of operations to break down the adhesions to meet the intimal conditions of embryo transfer is less than that of electric energy instruments. For the pregnancy rate after transplantation, the method of operation and the degree of intrauterine adhesion did not affect the pregnancy rate and live birth rate.
2023, 44(3): 131-137.
doi: 10.12259/j.issn.2095-610X.S20230311
Abstract:
Objective To investigate the relationship between bile duct uneven dilatation and postoperative pancreatitis after ERCP. Methods A total of 247 cases of ERCP+EST + ENBD due to bile duct stone were performed in the Second Affiliated Hospital of Kunming Medical University from January 2018 to December 2021. They were divided into the bile duct dilation group （bile duct diameter ≥10 mm） （group A = 84） and the nondilated group （bile duct diameter < 10 mm） （group B = 163） as control group for statistical processing and comparison. Results There were 84 patients in group A, including 45 females; 9 cases of suspected SOD; there are 30 patients with difficult cannulation, including 22 patients with pancreatic guidewire passages and 8 patients with development of the pancreatic duct; 9 cases of APBDJ.In group A, there were 10 cases of mild pancreatitis, 8 cases of moderate pancreatitis, and 3 cases of severe pancreatitis. There were 163 patients in group B, including 72 females; 11 cases of suspected SOD; there were 63 patients with difficult cannulation, including 61 pancreatic guidewire passages and 2 patients with development of the pancreatic duct; 1 case of APBDJ. In group B, there are 10 cases of mild pancreatitis, 1 case of moderate pancreastitis, and 4 cases of severe pancreatitis. There were 30 patients in group A and 57 patients in group B who received indomethacin anal embolus. The incidence of PEP was 25% in group A and 9.2% in group B, and the difference between the two groups was statistically significant （P = 0.001）. Logistic univariate regression analysis of 247 patients showed that bile duct dilatation （OR = 3.289, 95%CI = 1.593～6.792, P = 0.001） was an independent risk factor for PEP. Conclusions Female patient with uneven bile duct dilation （bile duct diameter ≥10 mm） is more likely to develop PEP than nondilated patient （bile duct diameter < 10 mm）. Therefore, ERCP + EST + ENBD should be carefully handled in these patients, and complex endoscopic surgical operations should be avoided as far as possible.
2023, 44(3): 138-142.
doi: 10.12259/j.issn.2095-610X.S20230318
Abstract:
Objective To evaluate the clinical application of fat emulsion amino acid （17） glucose （11%） injection （trade name: Calvin） to improve the standardization and rationality of it’s clinical application. Methods Using the hospital information system （Hiss） , 30 medical records were randomly selected every quarter with 360 medical records from October 2019 to September 2022. Reference basis for review, such as “the instructions of drugs”, “ building guide ”, “Standard of parenteral nutrition therapy in Lincang People’s Hospital”, and “Evaluation criteria for special prescriptions of Fat Emulsion, Amino Acids （17） and Glucose （11% ） Injection （for trial implementation）”. Comments on the content: whether the indication of drug use is appropriate, whether the dosage of drug usage is appropriate, whether the drug are suitable, whether incompatibility of drugs is contraindicated, the right or wrong deployment sequence. Results Of the 360 medical records collected, 62 were unreasonable in the use of Calvin, with an overall unreasonable rate of 17.2% . The rates of inappropriate use of Calvin were 16.7% , 13.3% , 13.3% , 23.3% , 23.3% , 26.7% , 26.7% , 26.7% , 10.0% , 13.3% , 10.0% and 3.3% respectively. Conclusion According to the review, there are some problems in Cavan’s clinical use, such as not strictly grasping the indication of drug use, many kinds of drugs added to Calvin, unsuitable added drugs, unsuitable added drug dosage forms and excessive dosage, contraindication in compatibility, and wrong order of Calvin’s dispensing. It is suggested that pharmacists should increase the strength, intensity and depth of the evaluation of clinical use of Calvin so as to improve the standardization and rationality of its use.
2023, 44(3): 143-148.
doi: 10.12259/j.issn.2095-610X.S20230301
Abstract:
Suicide is a major global public health concern and is one of the leading causes of death worldwide, causing enormous socioeconomic losses and psychological damage to human beings. Major depressive disorder is the leading cause of death of suicide, and it is crucial to investigate the pathophysiological mechanisms of suicide by depression to prevent and reduce suicide. Suicide by depression is closely related to genetic, environmental, social, psychological, and biological factors. With the development of genomics technology, more and more researchers explore the genes related to suicide in depression. This study reviews recent genetic researches on suicide by depression to provide references for the study of its pathological mechanisms.
Suicide is a major global public health concern and is one of the leading causes of death worldwide, causing enormous socioeconomic losses and psychological damage to human beings. Major depressive disorder is the leading cause of death of suicide, and it is crucial to investigate the pathophysiological mechanisms of suicide by depression to prevent and reduce suicide. Suicide by depression is closely related to genetic, environmental, social, psychological, and biological factors. With the development of genomics technology, more and more researchers explore the genes related to suicide in depression. This study reviews recent genetic researches on suicide by depression to provide references for the study of its pathological mechanisms.
2023, 44(3): 149-154.
doi: 10.12259/j.issn.2095-610X.S20230307
Abstract:
Acyl-CoA Synthetase Medium Chains（ACSMs） are enzymes that can activate C6～C10 fatty acids and participate in the synthesis and decomposition of medium chain fatty acids. They are of great significance for cell survival. When tumor occurs, due to the rapid growth of tumor cells, more energy supply is required than that required by normal cells, except glycolysis, fatty acid energy supply is also an important way for tumor cells to obtain energy. The study of ACSMs, the key enzyme of medium chain fatty acid synthesis and metabolism, is of great significance to further explore the occurrence, development and prognosis of tumors. This review mainly introduces the research progress of ACSM family members and tumorigenesis and development in recent years.
Acyl-CoA Synthetase Medium Chains（ACSMs） are enzymes that can activate C6～C10 fatty acids and participate in the synthesis and decomposition of medium chain fatty acids. They are of great significance for cell survival. When tumor occurs, due to the rapid growth of tumor cells, more energy supply is required than that required by normal cells, except glycolysis, fatty acid energy supply is also an important way for tumor cells to obtain energy. The study of ACSMs, the key enzyme of medium chain fatty acid synthesis and metabolism, is of great significance to further explore the occurrence, development and prognosis of tumors. This review mainly introduces the research progress of ACSM family members and tumorigenesis and development in recent years.
2023, 44(3): 155-159.
doi: 10.12259/j.issn.2095-610X.S20230323
Abstract:
In the maxillofacial region, tissue defects caused by tumors, trauma, congenital or acquired acute can cause serious damage to the patient’s appearance, normal physiological function and mental health, and overall quality of life of the patient. It is very important to find an appropriate treatment method to restore these defective tissues. In recent years, with the rapid development of tissue engineering, people attach great importance to oral tissue regeneration technology. Bone marrow mesenchymal stem cells （BMSCs）are most often used in regenerative medicine because of their potential to differentiate into other cells. In recent years, researchers have developed many biological scaffolds based on BMSCs to meet the needs of oral and maxillofacial tissue engineering with their excellent performance. This review introduces the research progress and application of BMSCs in stomatology, and discusses its application prospect in stomatology.
In the maxillofacial region, tissue defects caused by tumors, trauma, congenital or acquired acute can cause serious damage to the patient’s appearance, normal physiological function and mental health, and overall quality of life of the patient. It is very important to find an appropriate treatment method to restore these defective tissues. In recent years, with the rapid development of tissue engineering, people attach great importance to oral tissue regeneration technology. Bone marrow mesenchymal stem cells （BMSCs）are most often used in regenerative medicine because of their potential to differentiate into other cells. In recent years, researchers have developed many biological scaffolds based on BMSCs to meet the needs of oral and maxillofacial tissue engineering with their excellent performance. This review introduces the research progress and application of BMSCs in stomatology, and discusses its application prospect in stomatology.
2023, 44(3): 160-166.
doi: 10.12259/j.issn.2095-610X.S20230319
Abstract:
Objective To understand the status quo and correlation of eating knowledge and skills needs of caregivers of patients with dysphagia after stroke, and to provide reference for medical workers to carry out targeted nursing training for caregivers. Methods From November 2021 to May 2022, 302 caregivers of patients with dysphagia after stroke were selected from two tertiary hospitals in Yunnan Province by convenience sampling. Data were analyzed using median and interquartile range M （P25, P75） descriptions, Mann-Whitney U test, Kruskal-Wallis test, and Spearman correlation. Results The dimensions in the questionnaire ranked from high to low as swallowing training method [5.00 （4.80, 5.00）], oral eating knowledge [5.00 （4.69, 5.00）], dysphagia knowledge [5.00 （4.60, 5.00）], tube feeding nursing knowledge [4.71 （4.35, 4.84）], complication nursing [4.67 （4.50, 5.00）], and nutrition knowledge [4.50 （4.16, 4.83）]. The total score of the demand questionnaire was [222 （213, 228）]. Conclusion Caregivers of stroke patients with dysphagia have a high demand for eating knowledge and skills. Medical workers should pay attention to the caregiver population of patients with dysphagia and give intervention measures according to the actual needs of caregivers, so as to promote the rehabilitation of patients.
2023, 44(3): 167-171.
doi: 10.12259/j.issn.2095-610X.S20230310
Abstract:
Objective To analyze the effect of white noise intervention in neonatal peripheral venipuncture pain. Methods A total of 135 hospitalized neonates from September 2022 to November 2022 in the Pediatric Newborn Intensive Care Unit of a hospital in Yunnan Province were included. They were randomly divided into a white noise group, a classical music group and a control group, with 45 cases in each group according to a random number table. White noise and classical music were played during peripheral venipuncture, while the control group was treated with routine tapping and pacifying intervention. Neonatal pain assessment score, heart rate, crying time and other outcome indicators were compared among the three groups. Results Compared with the control group, the pain scores at the time of peripheral venipuncture and 30 s, 60 s, 90 s and 120 s after venipuncture in the white noise group and the classical music group were significantly reduced and the differences were statistically significant （P < 0.05）. The pain scores at the time of peripheral venipuncture, 60 s and 90 s after puncture in the white noise group were lower than those in the classical music group （P < 0.05）. The heart rates at 30 s, 60 s, 90 s and 120 s after peripheral venipuncture in the white noise group and the classical music group were significantly lower than those in the control group （P < 0.05）. The heart rates of 30 s, 60 s and 90 s after peripheral venipuncture in the white noise group were lower than those in the classical music group （P < 0.05）. The neonatal crying time during peripheral vein puncture in the white noise group and the classical music group was shorter than that in the control group （P < 0.05）. The neonatal crying time during peripheral venipuncture in the white noise group was shorter than that in the classical music group （P < 0.05）. Conclusion White noise can effectively relieve pain and shorten crying time in neonatal peripheral venous puncture, and it is recommended to be applied to neonatal pain management.
2023, 44(3): 172-178.
doi: 10.12259/j.issn.2095-610X.S20230326
Abstract:
Objective To investigate the effect of rehabilitation training based on mechanical concepts combined with Ilizarov technical orthopedics on knee joint function, stability and lower limb weight bearing in elderly patients with knee osteoarthritis. Methods This study is a retrospective clinical study. 120 patients with knee osteoarthritis over 60 years old who were admitted to the Department of Orthopedics and Traumatology in our hospital from December 2019 to June 2021 were selected as the research subjects. All patients were divided into observation group and control group, according to different treatment methods. The control group received Ilizarov technical orthopedic intervention, and the observation group received rehabilitation training based on mechanics concepts combined with Ilizarov technical orthopedic intervention. We measured and compared the knee function of the two groups of patients before and after surgery at different periods （3 months, 6 months, 12 months） [American Knee Society Scale （KSS） score, American Hospital for Special Surgery （HSS） Knee Joint function system score, knee range of motion （ROM） ], pain level [visual analog scale （VAS） for pain], lower extremity weight-bearing mechanical axis [hip-knee-ankle angle （HKA） and tibiofemoral angle （FTA）] . Results The KSS scores of patients in the two groups continued to increase in different aspects such as stability, range of motion and total score at 3 months, 6 months and 12 months after operation, while the pain, defect deduction and other dimensions continued to decrease, and there were differences between the groups. Significant （P < 0.05）. The knee ROM and HSS scores of the two groups continued to increase at 3 months, 6 months, and 12 months after operation, while the VAS score continued to decrease, and the difference between the two groups was significant （P < 0.05）. At 3 months, 6 months, and 12 months after the operation, the HKA of the lower extremity weight-bearing position continued to increase, and the FTA continued to decrease, and the differences between the two groups were significant （P < 0.05）. Conclusion Rehabilitation training based on the mechanics concept combined with Ilizarov technical orthopedics can quickly relieve the pain symptoms of elderly patients with knee osteoarthritis, improve their knee joint mobility and stability, correct the mechanical axis of the lower limbs’ weight-bearing position, and improve knee joint function.
