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RSS2017 Vol. 38, No. 10
2017, 38(10): 1-5.
Abstract:
Objective To investigate the effect of traumatic occlusion on the development of condylar.Methods Twenty-seven male SD rats were randomly divided into three groups, the traumatic side, the opposite side and blank control group. The rats of experiment group were perpormed to bond 1 mm steel-wire on left upper molar, the blank group were breeding normally. The condylar was dissected when the rats were 1, 3, 5 weeks after experiment. The histology section was used to observe the morphology of condylar. The immunohistochemical staining was used to examine the PCNA positive cell, TUNEL was chosen to detect the apoptotic cell.Results The histology section showed there was no significant difference between the three groups, the immunochemical stain and TUNEL showed there was no significant difference in the proliferated and apoptotic cells with 1, 5 week after experiment; the proliferated cell number decreased while the apoptotic cell increased on the traumatic side with 3 weeks after experiment, there was significant difference between three groups. Conclusion One side traumatic occlusion has no significant effect on condylar morphology, however has some influence on cell growth, it suggests that traumatic occlusion to some extent has no obvious effect on condylar growth.
Objective To investigate the effect of traumatic occlusion on the development of condylar.Methods Twenty-seven male SD rats were randomly divided into three groups, the traumatic side, the opposite side and blank control group. The rats of experiment group were perpormed to bond 1 mm steel-wire on left upper molar, the blank group were breeding normally. The condylar was dissected when the rats were 1, 3, 5 weeks after experiment. The histology section was used to observe the morphology of condylar. The immunohistochemical staining was used to examine the PCNA positive cell, TUNEL was chosen to detect the apoptotic cell.Results The histology section showed there was no significant difference between the three groups, the immunochemical stain and TUNEL showed there was no significant difference in the proliferated and apoptotic cells with 1, 5 week after experiment; the proliferated cell number decreased while the apoptotic cell increased on the traumatic side with 3 weeks after experiment, there was significant difference between three groups. Conclusion One side traumatic occlusion has no significant effect on condylar morphology, however has some influence on cell growth, it suggests that traumatic occlusion to some extent has no obvious effect on condylar growth.
2017, 38(10): 6-10.
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Objective To study the effect of methamphetamine on the expression of DA, 5-HTand TNF-αin rats hippocampus. Methods (1) The in vivo toxicity model was established by injecting MA 15 mg/ (kg.time) , two times a day for 5 days. The in vitro toxicity model was established by incubating MA with mixed hippocampal neurons/microglia for 24 hours. (2) ELISA was used to detect the content of DA, 5-HT and TNF-α in rat hippocampus and cell culture supernatant. Results On the fifth day, Methamphetamine treated rats showed hair errecting, weight loss, rapid head shaking, circling or head dorsal movement, with a stereotyped score of 5. The levels of DA and 5-HT decreased, and the content of TNF-alpha elevated in rats hippocampus of MA group. The levels of DA, 5-HT decreased and the content of TNF-alpha increased in cell cultural supernatants of MA group24 hours. Conclusion The changes of DA, 5-HT and TNF-alpha are involved in rat hippocampal damage induced by methamphetamine.
Objective To study the effect of methamphetamine on the expression of DA, 5-HTand TNF-αin rats hippocampus. Methods (1) The in vivo toxicity model was established by injecting MA 15 mg/ (kg.time) , two times a day for 5 days. The in vitro toxicity model was established by incubating MA with mixed hippocampal neurons/microglia for 24 hours. (2) ELISA was used to detect the content of DA, 5-HT and TNF-α in rat hippocampus and cell culture supernatant. Results On the fifth day, Methamphetamine treated rats showed hair errecting, weight loss, rapid head shaking, circling or head dorsal movement, with a stereotyped score of 5. The levels of DA and 5-HT decreased, and the content of TNF-alpha elevated in rats hippocampus of MA group. The levels of DA, 5-HT decreased and the content of TNF-alpha increased in cell cultural supernatants of MA group24 hours. Conclusion The changes of DA, 5-HT and TNF-alpha are involved in rat hippocampal damage induced by methamphetamine.
2017, 38(10): 11-15.
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Objective To study the synthesis of dammarane-type triterpene derivatives by using Panaxadiol and Panaxatriol as starting materials and screen their cytotoxic activity in vitro. Methods The products derived from PCC oxidation and DDQ oxidation of panaxadiol and panaxatriol, were further oxidized with m-chloroperoxybenzoic acid (m-CPBA) , as a result, four ring opening products were obtained, which were subjected to in vitro cytotoxic activity screening with MTT method. Results Four ring-opening derivatives were prepared (Compound2, 4, 6, 8) . Conclusion The four ring-opening derivatives are new compounds, and compound 2 and 4 show significant cytotoxicity against several cancer cell lines.
Objective To study the synthesis of dammarane-type triterpene derivatives by using Panaxadiol and Panaxatriol as starting materials and screen their cytotoxic activity in vitro. Methods The products derived from PCC oxidation and DDQ oxidation of panaxadiol and panaxatriol, were further oxidized with m-chloroperoxybenzoic acid (m-CPBA) , as a result, four ring opening products were obtained, which were subjected to in vitro cytotoxic activity screening with MTT method. Results Four ring-opening derivatives were prepared (Compound2, 4, 6, 8) . Conclusion The four ring-opening derivatives are new compounds, and compound 2 and 4 show significant cytotoxicity against several cancer cell lines.
2017, 38(10): 16-21.
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Objectives To identify Radix Saussureae lappae from different habitats by application of HPLC fingerprints and chemometrics. Methods By using reversed phase high performance liquid chromatography (HPLC) , the analysis was carried out with the mobile phases of Acetonitrile and water by gradient elution. The flow rate was 1.0 m L/min, and the UV detection wave length was 254 nm. Similarity calculation, principal component analysis and hierarchical cluster analysis were applied to read HPLC fingerprints, then to identify of Radix Saussureae lappae from different habitats. Results The method could be used to separate the components of Radix Saussureae lappae. According to the test results, 18 common fingerprint peaks were assigned, and a common HPLC fingerprint pattern of Radix Saussureae lappae was established, and the similarity of Radix Saussureae lappae from 15 different sources was high. But there were significant differences in the fingerprint patterns of samples from different habitats. Conclusion With excellent specificity and reproducibility, the fingerprinting method provides a feasible method for identification of Radix Saussureae lappae from different habitats, and can provide references for Radix Saussureae lappae quality control, and provide references for resources reasonable development and utilization of Radix Saussureae lappae.
Objectives To identify Radix Saussureae lappae from different habitats by application of HPLC fingerprints and chemometrics. Methods By using reversed phase high performance liquid chromatography (HPLC) , the analysis was carried out with the mobile phases of Acetonitrile and water by gradient elution. The flow rate was 1.0 m L/min, and the UV detection wave length was 254 nm. Similarity calculation, principal component analysis and hierarchical cluster analysis were applied to read HPLC fingerprints, then to identify of Radix Saussureae lappae from different habitats. Results The method could be used to separate the components of Radix Saussureae lappae. According to the test results, 18 common fingerprint peaks were assigned, and a common HPLC fingerprint pattern of Radix Saussureae lappae was established, and the similarity of Radix Saussureae lappae from 15 different sources was high. But there were significant differences in the fingerprint patterns of samples from different habitats. Conclusion With excellent specificity and reproducibility, the fingerprinting method provides a feasible method for identification of Radix Saussureae lappae from different habitats, and can provide references for Radix Saussureae lappae quality control, and provide references for resources reasonable development and utilization of Radix Saussureae lappae.
2017, 38(10): 22-26.
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Objective To screen small interfering ribonucleic acid (si RNA) that can effectively silence the vitamin D receptor (VDR) of human renal proximal tubular epithelial cells (HK-2 cells) and its significance for hypocitraturia. Methods We cultured HK-2 cells, designed 4 VDR-si RNA, used packaged lentivirus to infect HK-2 cells and detected the expression level of VDR protein and m RNA of HK-2 cells whose VDR gene silenced by Western blot and real-time quantitative PCR. Results Si RNA 1 (6120) could significantly inhibit the expression of VDR in HK-2 cells, and si RNA VDR-1 (6120) stable cell lines were obtained. Conclusion VDR-si RNA 1 (6120) can effectively silence the expression of VDR in HK-2 cells, and we can detect the expression of Na DC1 after VDR expression is inhibited in the next step.
Objective To screen small interfering ribonucleic acid (si RNA) that can effectively silence the vitamin D receptor (VDR) of human renal proximal tubular epithelial cells (HK-2 cells) and its significance for hypocitraturia. Methods We cultured HK-2 cells, designed 4 VDR-si RNA, used packaged lentivirus to infect HK-2 cells and detected the expression level of VDR protein and m RNA of HK-2 cells whose VDR gene silenced by Western blot and real-time quantitative PCR. Results Si RNA 1 (6120) could significantly inhibit the expression of VDR in HK-2 cells, and si RNA VDR-1 (6120) stable cell lines were obtained. Conclusion VDR-si RNA 1 (6120) can effectively silence the expression of VDR in HK-2 cells, and we can detect the expression of Na DC1 after VDR expression is inhibited in the next step.
2017, 38(10): 27-31.
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Objecctive To analyze the result of the pass ratio when we use different dose (2.5 μL, 5 μL and 10 μL) of PCR products to detect DNA methylation by pyrosequencing. Methods DNA extracted from blood samples that collected from 20 alcohol-dependent patients and 20 healthy controls were treated with Epi Tect Fast DNA Bisulfite Kit. PCR amplification was performed using converted DNA as template.We added different dose of PCR products (2.5 μL, 5 μL, 10 μL) to detecte DNA methylation quantification values of target gene. Results By comparing the average peak height and the pass ratio of pyrosequencing, we found the group (10 μL) was the maximum, the group (2.5 μL) was the maximum. The result of the average peak height and the pass ratio of three groups was increased significantly (P<0.05) . Conclusions When we treat 200 ng DNA extracted from blood samples with Epi Tect Fast DNA Bisulfite Kit, the group (10 μL) sequencing pass ratio is optimum. The sequencing pass ratio of the group (10 μL) is 100 %.
Objecctive To analyze the result of the pass ratio when we use different dose (2.5 μL, 5 μL and 10 μL) of PCR products to detect DNA methylation by pyrosequencing. Methods DNA extracted from blood samples that collected from 20 alcohol-dependent patients and 20 healthy controls were treated with Epi Tect Fast DNA Bisulfite Kit. PCR amplification was performed using converted DNA as template.We added different dose of PCR products (2.5 μL, 5 μL, 10 μL) to detecte DNA methylation quantification values of target gene. Results By comparing the average peak height and the pass ratio of pyrosequencing, we found the group (10 μL) was the maximum, the group (2.5 μL) was the maximum. The result of the average peak height and the pass ratio of three groups was increased significantly (P<0.05) . Conclusions When we treat 200 ng DNA extracted from blood samples with Epi Tect Fast DNA Bisulfite Kit, the group (10 μL) sequencing pass ratio is optimum. The sequencing pass ratio of the group (10 μL) is 100 %.
2017, 38(10): 32-35.
Abstract:
Objective To study the epidemiological characteristics of leprosy of the migrating population in Kunming, 2000.1-2015.12, and to provide basis for Source tracking in the future. Methods Date were collected from the Leprosy Management Information System in China (LEPMIS) from 2000.1 to 2015.12 in Kunming. A descriptive and comparative analysis were performed.Results 383 new leprosy cases of the migrating population and 38 new leprosy cases of the migrating population were detected from 2001.1 to 2015.12, 84.3% and 8.4%, respectively, the difference was significant (P<0.01) ;Among the new leprosy cases of migrating population, 2.6% were children under 15 years, 92.1% multibacillary, and 39.5% suffered from grade 2 disability;The positive rates were 30.5% and 44.7% respectively between the registered population and tne migrating population, the difference was significant (P <0.05) .Conclusion This study will provide the evidence of leprosy control strategies by tracing the source of infection of the leprosy in the migrating population.
Objective To study the epidemiological characteristics of leprosy of the migrating population in Kunming, 2000.1-2015.12, and to provide basis for Source tracking in the future. Methods Date were collected from the Leprosy Management Information System in China (LEPMIS) from 2000.1 to 2015.12 in Kunming. A descriptive and comparative analysis were performed.Results 383 new leprosy cases of the migrating population and 38 new leprosy cases of the migrating population were detected from 2001.1 to 2015.12, 84.3% and 8.4%, respectively, the difference was significant (P<0.01) ;Among the new leprosy cases of migrating population, 2.6% were children under 15 years, 92.1% multibacillary, and 39.5% suffered from grade 2 disability;The positive rates were 30.5% and 44.7% respectively between the registered population and tne migrating population, the difference was significant (P <0.05) .Conclusion This study will provide the evidence of leprosy control strategies by tracing the source of infection of the leprosy in the migrating population.
2017, 38(10): 36-39.
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Objective To survey the family medical expense of rural residents and health resoueces access, to explore the main factors raising the family medical expense. Methods Stratified cluster random sampling method was used to investigate 3 659 rural families with 15309 residents in Chuxiong prefecture. Results The median of family annual medical expense was RMB 1 987.5 yuan. 65.1% of households from the nearest hospital within 1 km, which was higher than the average level of FNHSS. 70.6% of households from the nearest hospital within 10 minutes, which was lower than the national average level. A Muitiple Linear Regression Analysis indicated that the time and distance from home to the nearest hospital, the persons with two week prevalence without consultation rate, the persons with two week consultation rate and the persons with annual hospitalization rate were positively associated with the high the family medical expense. Conclusions Rural health services can basically meet the people's needs. Although the difficulty in getting health services is being ameliorated, the medical charge is still the main restricting factor of utilization rate. It is suggested that the health education should be strengthened in rural areas, the accessibility of health services be improved, and the residents' health differences among regions should be reduced.
Objective To survey the family medical expense of rural residents and health resoueces access, to explore the main factors raising the family medical expense. Methods Stratified cluster random sampling method was used to investigate 3 659 rural families with 15309 residents in Chuxiong prefecture. Results The median of family annual medical expense was RMB 1 987.5 yuan. 65.1% of households from the nearest hospital within 1 km, which was higher than the average level of FNHSS. 70.6% of households from the nearest hospital within 10 minutes, which was lower than the national average level. A Muitiple Linear Regression Analysis indicated that the time and distance from home to the nearest hospital, the persons with two week prevalence without consultation rate, the persons with two week consultation rate and the persons with annual hospitalization rate were positively associated with the high the family medical expense. Conclusions Rural health services can basically meet the people's needs. Although the difficulty in getting health services is being ameliorated, the medical charge is still the main restricting factor of utilization rate. It is suggested that the health education should be strengthened in rural areas, the accessibility of health services be improved, and the residents' health differences among regions should be reduced.
2017, 38(10): 40-44.
Abstract:
Objective To observe the pathological morphology changes of DCD donor liver, and to explore the application of DCD donor liver, especially steatosis, donor liver in allogeneic orthotopic liver transplantation (AOLT) . Methods 76 cases of DCD donor liver in AOLT were retrospectively studied, in which the grades of steatosis are classified as no steatosis (S0, n=36 cases) , mild (S1, n=21 cases) , moderate (S2, n= 14 cases) , and severe ones (S3 grade, n=5 cases) . The main influencing indexes of hepatic and renal function and the occurrence of early postoperative complications were observed at 3 days and 14 days after operation. Results Compared with the control group, the hepatic and renal function of S1 and S2 steathepatitis groups were not statistically significant at 3 days and 14 days after operation. The difference of the hepatic and renal function between S3 level and the control group was statistically significant. When compared with the control group, the incidence of complications of S1 and S2 group such as hemorrhage, infection, hepatic artery embolization, ascites and sepsis was not statistically significant (P>0.05) . There was statistically significant difference of the bleeding and infection, Hepatic artery embolism, ascites, sepsis and other complications between S3 level and the control group (P <0.05) . The survival rate of the patients in the 14 days postoperative was 94.4% in the control group and 90.5%, 85.7% and 60% in S1 group, S2 group and S3 group respectively. Conclusions Mild and moderate steatosis DCD donor liver can be used for transplantation in the case of careful selection. But it is difficult to overcome the risk factors for severe steatosis DCD donor liver for transplantation. So severe steatosis DCD donor liver should try to not to use.
Objective To observe the pathological morphology changes of DCD donor liver, and to explore the application of DCD donor liver, especially steatosis, donor liver in allogeneic orthotopic liver transplantation (AOLT) . Methods 76 cases of DCD donor liver in AOLT were retrospectively studied, in which the grades of steatosis are classified as no steatosis (S0, n=36 cases) , mild (S1, n=21 cases) , moderate (S2, n= 14 cases) , and severe ones (S3 grade, n=5 cases) . The main influencing indexes of hepatic and renal function and the occurrence of early postoperative complications were observed at 3 days and 14 days after operation. Results Compared with the control group, the hepatic and renal function of S1 and S2 steathepatitis groups were not statistically significant at 3 days and 14 days after operation. The difference of the hepatic and renal function between S3 level and the control group was statistically significant. When compared with the control group, the incidence of complications of S1 and S2 group such as hemorrhage, infection, hepatic artery embolization, ascites and sepsis was not statistically significant (P>0.05) . There was statistically significant difference of the bleeding and infection, Hepatic artery embolism, ascites, sepsis and other complications between S3 level and the control group (P <0.05) . The survival rate of the patients in the 14 days postoperative was 94.4% in the control group and 90.5%, 85.7% and 60% in S1 group, S2 group and S3 group respectively. Conclusions Mild and moderate steatosis DCD donor liver can be used for transplantation in the case of careful selection. But it is difficult to overcome the risk factors for severe steatosis DCD donor liver for transplantation. So severe steatosis DCD donor liver should try to not to use.
2017, 38(10): 45-49.
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Objective To investigate the antibiotic resistance of clinical strains of Haemophilus in fluenzae (HI) isolated from the First Affiliated Hospital of Kunming Medical University, to provide scientific data for using antibiotic in clinic rationally. Methods Clinical strains of HI were collected from January2012 to December 2015, Kirby-Bauer method was used to antimicrobial susceptibility test, beta-lactamases were detected by nitrocefin disk test. Data from antibiotic resistance of HI were analyzed by WHONET5.6. Results Among the 184 clinical strains of HI, the resistance rate to ampicillin, chloramphenicol, ampicillin\sulbactam was 46.7%, 23.2%, 13%, respectively. About 90% of these HI strains were still susceptible to azithromvcin, ciprofloxacin, meropenem, cefuroxime, cefotaxime. The resistance rate to ampicillin in the HI strains from children (56.9%) were higher than in the strains from adults (42.1%) , the resistance rate to ciprofloxacin, chloramphenicol, meropenem was in HI strains from children, significantly higher than in the strains from adults. The overall prevalence of beta-lactamases was 52.8% in the HI strains. The prevalence of beta-lactamases (64.3%) in the strains from children was higher than the prevalence of beta-lactamases (47.6%) in the strains from adults. There are one HIstrain resistant to ampicillin but beta-lactamases negative. Conclusions HI was mainly isolated from respiratory specimen and hospitalized patients. Ampicillin is inapplicable to empiric treatment for HI. Cephalosporin antibiotic, macrolides, enzyme inhibitor, quinlone, carbapenemases are stably susceptible. Beta-lactamase is the important mechanism of resistance.
Objective To investigate the antibiotic resistance of clinical strains of Haemophilus in fluenzae (HI) isolated from the First Affiliated Hospital of Kunming Medical University, to provide scientific data for using antibiotic in clinic rationally. Methods Clinical strains of HI were collected from January2012 to December 2015, Kirby-Bauer method was used to antimicrobial susceptibility test, beta-lactamases were detected by nitrocefin disk test. Data from antibiotic resistance of HI were analyzed by WHONET5.6. Results Among the 184 clinical strains of HI, the resistance rate to ampicillin, chloramphenicol, ampicillin\sulbactam was 46.7%, 23.2%, 13%, respectively. About 90% of these HI strains were still susceptible to azithromvcin, ciprofloxacin, meropenem, cefuroxime, cefotaxime. The resistance rate to ampicillin in the HI strains from children (56.9%) were higher than in the strains from adults (42.1%) , the resistance rate to ciprofloxacin, chloramphenicol, meropenem was in HI strains from children, significantly higher than in the strains from adults. The overall prevalence of beta-lactamases was 52.8% in the HI strains. The prevalence of beta-lactamases (64.3%) in the strains from children was higher than the prevalence of beta-lactamases (47.6%) in the strains from adults. There are one HIstrain resistant to ampicillin but beta-lactamases negative. Conclusions HI was mainly isolated from respiratory specimen and hospitalized patients. Ampicillin is inapplicable to empiric treatment for HI. Cephalosporin antibiotic, macrolides, enzyme inhibitor, quinlone, carbapenemases are stably susceptible. Beta-lactamase is the important mechanism of resistance.
2017, 38(10): 50-54.
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Objective To study the changes of myocardial injury markers hs-c Tn T, NT-pro BNP and CK-MB levels in CKD patients without dialysis in different stags and their value for outcome prediction. Methods A total of 137 cases of non-dialysis patients with chronic kidney disease were enrolled in this cross-sectional study.The myocardial injury markers hs-c Tn T, NT-pro BNP, CK-MBH and cardiac ultrasonography were examined. And the area under ROC was measured and compared to evaluate the value of different biomarkers in predicting cardiac dysfunction and outcome. Results The levels of plasma hs-c Tn T, NT-pro BNP and CK-MB in CKD5 group were the highest in the three groups, and the biomarlers in CKD3-4 group was significantly higher than CKD1-2 group (P<0.001) . The expression of LVH and the E/A <1, LVH in CKD5 group and CKD3-4 group were lower than CKD1-2 (P<0.001) .The levels of plasma hs-c Tn T, NT-pro BNP and CK-MB in the patients with CKD were measured and the left ventricular hypertrophy was evaluated by the curve of the working curve of the subjects.Through the ROC curve, the CK-MB had the highest accuracy of left ventricular hypertrophy, followed by NT-pro BNP and hs-c Tn T. Conlusions Levels of hs-c Tn T, NT-pro BNP and CK-MB are elevated in patients with CKD. CK-MB is a good biomarker in predicting cardic dysfunction of CKD patients with early cardiovascular disease.
Objective To study the changes of myocardial injury markers hs-c Tn T, NT-pro BNP and CK-MB levels in CKD patients without dialysis in different stags and their value for outcome prediction. Methods A total of 137 cases of non-dialysis patients with chronic kidney disease were enrolled in this cross-sectional study.The myocardial injury markers hs-c Tn T, NT-pro BNP, CK-MBH and cardiac ultrasonography were examined. And the area under ROC was measured and compared to evaluate the value of different biomarkers in predicting cardiac dysfunction and outcome. Results The levels of plasma hs-c Tn T, NT-pro BNP and CK-MB in CKD5 group were the highest in the three groups, and the biomarlers in CKD3-4 group was significantly higher than CKD1-2 group (P<0.001) . The expression of LVH and the E/A <1, LVH in CKD5 group and CKD3-4 group were lower than CKD1-2 (P<0.001) .The levels of plasma hs-c Tn T, NT-pro BNP and CK-MB in the patients with CKD were measured and the left ventricular hypertrophy was evaluated by the curve of the working curve of the subjects.Through the ROC curve, the CK-MB had the highest accuracy of left ventricular hypertrophy, followed by NT-pro BNP and hs-c Tn T. Conlusions Levels of hs-c Tn T, NT-pro BNP and CK-MB are elevated in patients with CKD. CK-MB is a good biomarker in predicting cardic dysfunction of CKD patients with early cardiovascular disease.
2017, 38(10): 55-59.
Abstract:
Objective To investigate the related problems in the emergency treatment of cardiac arrest in patients with early cardiac arrhythmias after valve replacement, and to provide reference for clinical practice.Methods 35 cases of cardiac arrest induced by early malignant ventricular arrhythmia after heart valve replacement in our department from January 2013 to January 2015 were selected as A group, 35 cases of cardiac arrest induced by early malignant ventricular arrhythmia after heart valve replacement in February 2015 to February 2017 were selected as the B group. In the study, treatment and nursing data of patients in A group were retrospectively analyzed, the nursing measures were carefully analyzed, and the clinical experience and shortcomings were summarized, the nursing process, quality standard and professional training for the cardiac arrest patients induced by malignant arrhythmia were reanalyzed, and formulated a standard, normal, scientific and effective management system. Then we applied the new management system in B group. We compared the sinus rhythm recovery time, the autonomous blood pressure recovery time, the autonomous breathing recovery time, the cases of successful rescue, the chest compression time, the defibrillation frequency, the types of used antiarrhythmic drug, the cases of bedside thoracotomy, the cases of using temporary pacemaker, the mechanical ventilation time and the prognosis of the patients. Results The sinus rhythm recovery time, the autonomous blood pressure recovery time, the autonomous breathing recovery time, the chest compression time, and the mechanical ventilation time of patients in B group were lower than the A group, the preliminary rescue success rate, the success rate of rescue in B group were higher than the A group, the difference was statistically significant (P < 0.05) . Conclusions For early rescue of cardiac arrest caused by malignant arrhythmia after valve replacement, the key is to strengthen the observation, early detect, give timely defibrillation, regulate the use of intravenous catheters and temporary pacemaker antiarrhythmic drugs and pay attention to use the central venous catheter and the temporary pacemaker.
Objective To investigate the related problems in the emergency treatment of cardiac arrest in patients with early cardiac arrhythmias after valve replacement, and to provide reference for clinical practice.Methods 35 cases of cardiac arrest induced by early malignant ventricular arrhythmia after heart valve replacement in our department from January 2013 to January 2015 were selected as A group, 35 cases of cardiac arrest induced by early malignant ventricular arrhythmia after heart valve replacement in February 2015 to February 2017 were selected as the B group. In the study, treatment and nursing data of patients in A group were retrospectively analyzed, the nursing measures were carefully analyzed, and the clinical experience and shortcomings were summarized, the nursing process, quality standard and professional training for the cardiac arrest patients induced by malignant arrhythmia were reanalyzed, and formulated a standard, normal, scientific and effective management system. Then we applied the new management system in B group. We compared the sinus rhythm recovery time, the autonomous blood pressure recovery time, the autonomous breathing recovery time, the cases of successful rescue, the chest compression time, the defibrillation frequency, the types of used antiarrhythmic drug, the cases of bedside thoracotomy, the cases of using temporary pacemaker, the mechanical ventilation time and the prognosis of the patients. Results The sinus rhythm recovery time, the autonomous blood pressure recovery time, the autonomous breathing recovery time, the chest compression time, and the mechanical ventilation time of patients in B group were lower than the A group, the preliminary rescue success rate, the success rate of rescue in B group were higher than the A group, the difference was statistically significant (P < 0.05) . Conclusions For early rescue of cardiac arrest caused by malignant arrhythmia after valve replacement, the key is to strengthen the observation, early detect, give timely defibrillation, regulate the use of intravenous catheters and temporary pacemaker antiarrhythmic drugs and pay attention to use the central venous catheter and the temporary pacemaker.
2017, 38(10): 60-64.
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Objective To determine the frequency of gene and the association of VDR gene polymorphismswith nephrotic syndrome (NS) and steroid responsiveness in Yun Nan children, so as to guide the use of hormone and immune inhibitor in this disease.Methods The subjects of the study were the 70 cases of children with nephrotic syndrome in pediatrics in the first affiliated hospital of kunming medical university from2015, May to 2016, November. Genotypes of the VDR TaqI gene polymorphism were analyzed using the PCR amplification product enzyme and agarose gel electrophoresis.Results A total of 70 NS (42 steroid sensitive [SS]and 28 steroid resistant [SR]) patientswith a mean age of 9.14 ±3.80 years were studied. The TT genotypeof VDR-TaqI polymorphism was detected in 57.1% of the SS patients compared to 57.1% of SR patients (P=1.000) .The Tt genotype was detected in 26.2% of SS patientscompared to 25.0% of SR patients (P =0.911) . The tt genotype was detected in 16.7% of SS patients compared to 17.9% of SR patients (P =0.897) . The T-allele frequency was 70.2% in SS patients compared to 69.6% of SR patients.The t-allele frequency was 29.8% in SS patients compared to 30.4% of SR patients (P =0.096) .No significant difference was detected in terms of VDR-Apal polymorphism in SS patients compared to SR patientsfor all the three genotypes (P =1.000, P =0.911, and P=0.897) . No significant difference was found in any of the allele frequencies between SS and SR (P =0.096) .Conclusion Vitamin D Receptor Gene TaqI polymorphisms has no correlation with the steroid responsiveness in childhood nephrotic syndrome.
Objective To determine the frequency of gene and the association of VDR gene polymorphismswith nephrotic syndrome (NS) and steroid responsiveness in Yun Nan children, so as to guide the use of hormone and immune inhibitor in this disease.Methods The subjects of the study were the 70 cases of children with nephrotic syndrome in pediatrics in the first affiliated hospital of kunming medical university from2015, May to 2016, November. Genotypes of the VDR TaqI gene polymorphism were analyzed using the PCR amplification product enzyme and agarose gel electrophoresis.Results A total of 70 NS (42 steroid sensitive [SS]and 28 steroid resistant [SR]) patientswith a mean age of 9.14 ±3.80 years were studied. The TT genotypeof VDR-TaqI polymorphism was detected in 57.1% of the SS patients compared to 57.1% of SR patients (P=1.000) .The Tt genotype was detected in 26.2% of SS patientscompared to 25.0% of SR patients (P =0.911) . The tt genotype was detected in 16.7% of SS patients compared to 17.9% of SR patients (P =0.897) . The T-allele frequency was 70.2% in SS patients compared to 69.6% of SR patients.The t-allele frequency was 29.8% in SS patients compared to 30.4% of SR patients (P =0.096) .No significant difference was detected in terms of VDR-Apal polymorphism in SS patients compared to SR patientsfor all the three genotypes (P =1.000, P =0.911, and P=0.897) . No significant difference was found in any of the allele frequencies between SS and SR (P =0.096) .Conclusion Vitamin D Receptor Gene TaqI polymorphisms has no correlation with the steroid responsiveness in childhood nephrotic syndrome.
2017, 38(10): 65-69.
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Objective To study the molecular mechanism of the infection process of Pseudomonas aeruginosa in human middle ear epithelial cells (HMEECs) . Methods Real-time PCR and Western blotting method were used to detect the m RNA and protein levels of TGF-β1 and Smad2, and the phosphorylation of Smad7 in the infection process of Pseudomonas aeruginosaonhuman middle ear epithelial cells. Results Infection of Pseudomonas aeruginosa in human middle ear epithelial cells significantly increased the m RNA and protein levels of TGF-β1 and Smad2, and also inhibited the phosphorylation of Smad7. Mutation of Opr F and PKC inhibitor Bisindolylmaleimide I could derease the m RNA and protein levels of TGF-β1 and Smad2 and the enhanced the phosphorylation of Smad7 compared with Pseudomonasaeruginosa infected HMEECs. Conclusion Infection of Pseudomonas aeruginosaonhuman activates the TGF-β1 Smad signaling pathway in middle ear epithelial cells.
Objective To study the molecular mechanism of the infection process of Pseudomonas aeruginosa in human middle ear epithelial cells (HMEECs) . Methods Real-time PCR and Western blotting method were used to detect the m RNA and protein levels of TGF-β1 and Smad2, and the phosphorylation of Smad7 in the infection process of Pseudomonas aeruginosaonhuman middle ear epithelial cells. Results Infection of Pseudomonas aeruginosa in human middle ear epithelial cells significantly increased the m RNA and protein levels of TGF-β1 and Smad2, and also inhibited the phosphorylation of Smad7. Mutation of Opr F and PKC inhibitor Bisindolylmaleimide I could derease the m RNA and protein levels of TGF-β1 and Smad2 and the enhanced the phosphorylation of Smad7 compared with Pseudomonasaeruginosa infected HMEECs. Conclusion Infection of Pseudomonas aeruginosaonhuman activates the TGF-β1 Smad signaling pathway in middle ear epithelial cells.
2017, 38(10): 70-73.
Abstract:
Objective To evaluate the management efficacy of PDCA Cycle in reducing the incidence of oral mucositis in HSCT patients, so as to alleviate the pain of patients and improve the nursing quality. Methods 80 patients received HSCT in our hospital from September 2014 to December 2016 were treated with PDCA Cycle (n=42) or conventional treatments (n=38) . We focused on the infection factors, careful nursing, and gave the scientific feedback, timely summarization. Results PDCA Cycle reduced the incidence of oral mucositis in HSCT patients from 63.15% to 38.09% with a statistically significant difference (P<0.05) . Conclutions PDCA Cycle could improve the management quality of oral mucositis in HSCT patients, shorten the severity greatly and promote the satisfaction of patients, thus promote the recovery of the patients.
Objective To evaluate the management efficacy of PDCA Cycle in reducing the incidence of oral mucositis in HSCT patients, so as to alleviate the pain of patients and improve the nursing quality. Methods 80 patients received HSCT in our hospital from September 2014 to December 2016 were treated with PDCA Cycle (n=42) or conventional treatments (n=38) . We focused on the infection factors, careful nursing, and gave the scientific feedback, timely summarization. Results PDCA Cycle reduced the incidence of oral mucositis in HSCT patients from 63.15% to 38.09% with a statistically significant difference (P<0.05) . Conclutions PDCA Cycle could improve the management quality of oral mucositis in HSCT patients, shorten the severity greatly and promote the satisfaction of patients, thus promote the recovery of the patients.
2017, 38(10): 74-78.
Abstract:
Objective To investigate the application value of magnetic resonance enterography (MRE) with multiple sequences indetection of small bowel diseases. Methods Thirty-four patients with suspected small bowel diseases received MR enterography.The patients underwent MRE with multiple sequences including breath-hold T2-weighted turbo spin-echo sequence (T2 WI-TSE-BH) , diffusion weighted imaging (DWI) , Balanced steady-state free precession sequence (BTFE) , and enhanced 3 D-THRIVE scans.The side effects were recorded and the image quality was graded as bad, good and excellent. Then we compared the results between MR enterography and surgery, pathology or clinic was performed.Results Plain scan sequences of COR- (T2 WI-TSE-BH and DWI (b=800) resulted in the best image quality. Diffusion sequence was of great value for assessing enteritis activity, and augmented sequences led to high-quality images to meet the requirements of clinical diagnosis. Conclusion MRE with multiple sequences with more information, high sensitivity and non-ionizing radiation, can be used as an important method in diagnosis of intestinal diseases.
Objective To investigate the application value of magnetic resonance enterography (MRE) with multiple sequences indetection of small bowel diseases. Methods Thirty-four patients with suspected small bowel diseases received MR enterography.The patients underwent MRE with multiple sequences including breath-hold T2-weighted turbo spin-echo sequence (T2 WI-TSE-BH) , diffusion weighted imaging (DWI) , Balanced steady-state free precession sequence (BTFE) , and enhanced 3 D-THRIVE scans.The side effects were recorded and the image quality was graded as bad, good and excellent. Then we compared the results between MR enterography and surgery, pathology or clinic was performed.Results Plain scan sequences of COR- (T2 WI-TSE-BH and DWI (b=800) resulted in the best image quality. Diffusion sequence was of great value for assessing enteritis activity, and augmented sequences led to high-quality images to meet the requirements of clinical diagnosis. Conclusion MRE with multiple sequences with more information, high sensitivity and non-ionizing radiation, can be used as an important method in diagnosis of intestinal diseases.
2017, 38(10): 79-82.
Abstract:
Objective To select the best concentration, volume and checking time of Gd-DTPA dilution as an oral negative gastrointestinal contrast agent by in vitro/in vivo experiments. Methods (1) In vitro experiments :we used 0.05, 0.10, 0.15 and 0.20 m L intravenous injections of Gd-DTPA, which were diluted into concentrations of 0.25, 0.50, 0.75, 1.00 mmol/L, then we examined and made a contrastive analysis about the signal intensity and attenuation rate of 2 D-MRCP with different concentration contrast agent. (2) In vivo experiments: we used the optimum concentration of Gd-DTPA dilution with 50 and 100 m L, made 2 D-MRCP scanning on 20 cases in two groups in 4-6 minutes and 9-11 minutes respectively after oral administration of Gd-DTPA dilution, and observed the restraint of the high signal liquid in stomach and duodenum, and whether the patients had adverse reactions. Results (1) It showed that the high signal liquid in stomach and duodenum of some patients with oral administration of 100 ml dilution with 1.00 mmol/L concentration could be fully restrained in 9-11 minutes. (2) The allocation of oral Gd-DTPA dilution is simple, the effect of restraining the high signal liquid in stomach and duodenum is well after oral administration of little dilution, and the patients have no adverse reactions, so it is a good oral gastrointestinal contrast agent.Conclusions 2 D-MRCP scanning in suitable time after oral administration of Gd-DTPA dilution with proper concentration and volume can effectively restrain the high signal liquid in stomach and duodenum.
Objective To select the best concentration, volume and checking time of Gd-DTPA dilution as an oral negative gastrointestinal contrast agent by in vitro/in vivo experiments. Methods (1) In vitro experiments :we used 0.05, 0.10, 0.15 and 0.20 m L intravenous injections of Gd-DTPA, which were diluted into concentrations of 0.25, 0.50, 0.75, 1.00 mmol/L, then we examined and made a contrastive analysis about the signal intensity and attenuation rate of 2 D-MRCP with different concentration contrast agent. (2) In vivo experiments: we used the optimum concentration of Gd-DTPA dilution with 50 and 100 m L, made 2 D-MRCP scanning on 20 cases in two groups in 4-6 minutes and 9-11 minutes respectively after oral administration of Gd-DTPA dilution, and observed the restraint of the high signal liquid in stomach and duodenum, and whether the patients had adverse reactions. Results (1) It showed that the high signal liquid in stomach and duodenum of some patients with oral administration of 100 ml dilution with 1.00 mmol/L concentration could be fully restrained in 9-11 minutes. (2) The allocation of oral Gd-DTPA dilution is simple, the effect of restraining the high signal liquid in stomach and duodenum is well after oral administration of little dilution, and the patients have no adverse reactions, so it is a good oral gastrointestinal contrast agent.Conclusions 2 D-MRCP scanning in suitable time after oral administration of Gd-DTPA dilution with proper concentration and volume can effectively restrain the high signal liquid in stomach and duodenum.
2017, 38(10): 83-89.
Abstract:
Objective To investigate the effects of intravenous infusion of tranexamic acid (TXA) combined with epinephrine (EPI) on the early functional recovery of joints of patients with total unilateral artificial knee arthroplasty (TKA) . Methods A total of 122 patients with unilateral TKA treated with orthopedic treatment from February 2015 to December 2016 in Kunming Medical University were selected as the subjects. The random number table was used to divided them into four groups according to different methods. TXA + EPI group (n=31) :intravenous infusion of tranexamic acid (10 mg/kg) was given 10 min before the hemostatic tourniquet, and 50 ml (1: 200 000) of adrenaline dilution was injected into the joint cavity before the end of the suture capsule. (n=31) : intravenous infusion of tranexamic acid (10 mg/kg) was given to the tourniquet 10 min before the hemostatic tourniquet, and the drainage tube was clamped for 2 hours. The EPI group (n=30) : only after the injection of drainage tube 2 h (1: 200 000) , injection of the drainage tube after injection of 2 h;control group (n=30) :only after the closure of the drainage tube 2 h. The operative time, total blood loss, postoperative drainage volume, occult blood loss, postoperative blood transfusion rate, postoperative blood transfusion rate, postoperative thickness of 10 cm on patellar and patellofemps were observed on 3 to 5 days postoperation. The deep vein thrombosis7 d after surgery was observed by lower extremity vascular ultrasound examination, and patients were followed up for3 months. Rsults Four patients in the TXA + EPI group had an incisional healing and extended to 3 weeks. The remaining three groups of patients were wound healing, 2 weeks or so stitches. There was no significant difference in the HSS score between the four groups (P>0.05) . TXA + EPI group, TXA group, EPI group (P<0.05) , but there was no significant difference between the two groups (P <0.05) , but there was no significant difference between the two groups (P<0.05) , but the total blood loss, postoperative drainage volume, hidden blood loss, postoperative blood transfusion rate (P<0.05) . There was no significant difference between the EPI group and the rest group (P>0.05) . The level of TXA + EPI in the EPI group was higher than that in the other three groups (P>0.05) (P<0.05) Conclusion Tranexamic acid combined with adrenaline can significantly reduce the first unilateral artificial TKA postoperative blood loss, reduce the knee swelling after TKA, and promote the patient's early knee functional exercise, accelerate knee function recovery, there is a certain application value, but may cause delayed wound healing and other complications, clinical application need to be cautious.
Objective To investigate the effects of intravenous infusion of tranexamic acid (TXA) combined with epinephrine (EPI) on the early functional recovery of joints of patients with total unilateral artificial knee arthroplasty (TKA) . Methods A total of 122 patients with unilateral TKA treated with orthopedic treatment from February 2015 to December 2016 in Kunming Medical University were selected as the subjects. The random number table was used to divided them into four groups according to different methods. TXA + EPI group (n=31) :intravenous infusion of tranexamic acid (10 mg/kg) was given 10 min before the hemostatic tourniquet, and 50 ml (1: 200 000) of adrenaline dilution was injected into the joint cavity before the end of the suture capsule. (n=31) : intravenous infusion of tranexamic acid (10 mg/kg) was given to the tourniquet 10 min before the hemostatic tourniquet, and the drainage tube was clamped for 2 hours. The EPI group (n=30) : only after the injection of drainage tube 2 h (1: 200 000) , injection of the drainage tube after injection of 2 h;control group (n=30) :only after the closure of the drainage tube 2 h. The operative time, total blood loss, postoperative drainage volume, occult blood loss, postoperative blood transfusion rate, postoperative blood transfusion rate, postoperative thickness of 10 cm on patellar and patellofemps were observed on 3 to 5 days postoperation. The deep vein thrombosis7 d after surgery was observed by lower extremity vascular ultrasound examination, and patients were followed up for3 months. Rsults Four patients in the TXA + EPI group had an incisional healing and extended to 3 weeks. The remaining three groups of patients were wound healing, 2 weeks or so stitches. There was no significant difference in the HSS score between the four groups (P>0.05) . TXA + EPI group, TXA group, EPI group (P<0.05) , but there was no significant difference between the two groups (P <0.05) , but there was no significant difference between the two groups (P<0.05) , but the total blood loss, postoperative drainage volume, hidden blood loss, postoperative blood transfusion rate (P<0.05) . There was no significant difference between the EPI group and the rest group (P>0.05) . The level of TXA + EPI in the EPI group was higher than that in the other three groups (P>0.05) (P<0.05) Conclusion Tranexamic acid combined with adrenaline can significantly reduce the first unilateral artificial TKA postoperative blood loss, reduce the knee swelling after TKA, and promote the patient's early knee functional exercise, accelerate knee function recovery, there is a certain application value, but may cause delayed wound healing and other complications, clinical application need to be cautious.
2017, 38(10): 90-94.
Abstract:
Objective To evaluate the clinical outcome and discuss the method of treatment both-bone forearm fracture in children (4 to 15 years old) with elastic intramedullary nail (EIN) . Methods We retrospectively analyzed the clinical result of forty-five cases to treat both-bone forearm fracture in children (4 to 15 years old) with elastic intramedullary nail from April, 2008 to June, 2014.Then we Discussed and analyzed the complication, surgery technique, limb function. Patients were followed up for more than 24 to 30 months after the surgery to continuously evaluate the limb function and fracture healing by physical examination and x-ray image.Results There were 38 children with excellent recovery, 7 good and no fair by followed up for 24 to 30 months, 9 cases with observed complication due by prominent nail ends, 3 patients slightly declined after the surgery, 1 patient was observed with ulna fracture delayed recovery during 3 to 8 months of fracture healing. Apposition rate in all cases was>50% in anterior-posterior view and the apposition rate>90% in lateral view. No fracture of ESIN, Nerve injuries, severe displacement were observed during this study. Conclusion The elastic intramedullary nail is a good method to treat children both-bone forearm fracture with lower surgery technique, minimall invasion, if less complications, does not interfere with growth, has good forearm function recovery.
Objective To evaluate the clinical outcome and discuss the method of treatment both-bone forearm fracture in children (4 to 15 years old) with elastic intramedullary nail (EIN) . Methods We retrospectively analyzed the clinical result of forty-five cases to treat both-bone forearm fracture in children (4 to 15 years old) with elastic intramedullary nail from April, 2008 to June, 2014.Then we Discussed and analyzed the complication, surgery technique, limb function. Patients were followed up for more than 24 to 30 months after the surgery to continuously evaluate the limb function and fracture healing by physical examination and x-ray image.Results There were 38 children with excellent recovery, 7 good and no fair by followed up for 24 to 30 months, 9 cases with observed complication due by prominent nail ends, 3 patients slightly declined after the surgery, 1 patient was observed with ulna fracture delayed recovery during 3 to 8 months of fracture healing. Apposition rate in all cases was>50% in anterior-posterior view and the apposition rate>90% in lateral view. No fracture of ESIN, Nerve injuries, severe displacement were observed during this study. Conclusion The elastic intramedullary nail is a good method to treat children both-bone forearm fracture with lower surgery technique, minimall invasion, if less complications, does not interfere with growth, has good forearm function recovery.
2017, 38(10): 95-100.
Abstract:
Objective To investigate the clinical characteristics and surgical strategies of papillary thyroid carcinoma (PTC) combined with Hashimoto 's thyroiditis (HT) . Methods The retrospective study was performed in 82 patients of PTC with HT patients among 334 PTC patients. Results The results showed that all patients underwent surgical treatment. According to the patient's condition, we performed glandular and isthmus resection, total or near total resection and different range of cervical lymph node dissection. The postoperative pathology confirmed as PTC. Compared with the PTC group, the female in PTC+HT group's cervical lymph node negative ratio was significantly increased, and the maximum diameter of the lesion was significantly reduced (all P<0.05) . The rate of transient hypocalcemia was significantly higher in PTC+HT group than that in the PTC group (P <0.01) . Conclusions PTC combined with HT is more common in women. HT may have a certain inhibitory effect on the invasion of PTC primary tumor and lymph node metastasis. Surgery in PTC+HT may have higher incidence of complications which should be paid more attention to.
Objective To investigate the clinical characteristics and surgical strategies of papillary thyroid carcinoma (PTC) combined with Hashimoto 's thyroiditis (HT) . Methods The retrospective study was performed in 82 patients of PTC with HT patients among 334 PTC patients. Results The results showed that all patients underwent surgical treatment. According to the patient's condition, we performed glandular and isthmus resection, total or near total resection and different range of cervical lymph node dissection. The postoperative pathology confirmed as PTC. Compared with the PTC group, the female in PTC+HT group's cervical lymph node negative ratio was significantly increased, and the maximum diameter of the lesion was significantly reduced (all P<0.05) . The rate of transient hypocalcemia was significantly higher in PTC+HT group than that in the PTC group (P <0.01) . Conclusions PTC combined with HT is more common in women. HT may have a certain inhibitory effect on the invasion of PTC primary tumor and lymph node metastasis. Surgery in PTC+HT may have higher incidence of complications which should be paid more attention to.
2017, 38(10): 101-106.
Abstract:
Objective To investigate the serum expression of tumor markers in patients with colorectal cancer, and evaluate the value of combined detection of multiple tumor markers in clinical diagnosis. Methods The serum levels of 12 tumor markers (CA125, CA153, CA199, CA242, CEA, AFP, NSE, Free-PSA, PSA, β-HCG, HGH, Ferritin) in 134 patients with colorectal cancer and 293 healthy controls were analyzed by C-12 protein chip. The results were analyzed by statistical methods. Results The serum levels of tumor markers, including CEA, CA199, CA242, AFP, CA125, β-HCG in patients with colorectal cancer were significantly higher than the healthy controls (P<0.05) . The positive rate of C-12 protein chip was 74.63% in patients with colorectal cancer, significantly higher than the positive rates in healthy controls (10.24%) .CEA+CA199+CA242+CA125+AFP combined detection had the highest efficiency. Diagnostic sensitivity and specificity was greatly improved by combined detection. Conclusions Combined detection of multiple tumor markers has significant value for the diagnosis in the patients with colorectal cancer.CEA+CA199+CA242+CA125+AFP combined detection can be used as markers for colorectal cancer diagnosis, which may improve diagnostic efficiency.
Objective To investigate the serum expression of tumor markers in patients with colorectal cancer, and evaluate the value of combined detection of multiple tumor markers in clinical diagnosis. Methods The serum levels of 12 tumor markers (CA125, CA153, CA199, CA242, CEA, AFP, NSE, Free-PSA, PSA, β-HCG, HGH, Ferritin) in 134 patients with colorectal cancer and 293 healthy controls were analyzed by C-12 protein chip. The results were analyzed by statistical methods. Results The serum levels of tumor markers, including CEA, CA199, CA242, AFP, CA125, β-HCG in patients with colorectal cancer were significantly higher than the healthy controls (P<0.05) . The positive rate of C-12 protein chip was 74.63% in patients with colorectal cancer, significantly higher than the positive rates in healthy controls (10.24%) .CEA+CA199+CA242+CA125+AFP combined detection had the highest efficiency. Diagnostic sensitivity and specificity was greatly improved by combined detection. Conclusions Combined detection of multiple tumor markers has significant value for the diagnosis in the patients with colorectal cancer.CEA+CA199+CA242+CA125+AFP combined detection can be used as markers for colorectal cancer diagnosis, which may improve diagnostic efficiency.
2017, 38(10): 107-111.
Abstract:
Objective To analyse the characteristics of adverse reactions in children with acute lymphoblastic leukemia after chemotherapy, and to implement individualized medication to avoid or reduce the occurrence of serious adrs. Methods The report on the adverse reactions in children with acute lymphoblastic leukemia after chemotherapy from 2015.2 to 2014.3 was collected, the patient gender, age, risk, drug and ADR involving organs or systems were analyzed. Results The adverse reactions in children with acute lymphoblastic leukemia after chemotherapy are involved mostly in the high-risk group of patients. L-asparaginase, vincristine and cytarabine were the top three drugs involved in adverse reactions. Blood system was the most common system involved in adverse reactions. Bone marrow suppression concurrent infection had significant difference between different risks and ages (P < 0.01) had no statistical difference between different genders (P > 0.05) . Conclusion Bone marrow suppression was the most common ADR in children with acute lymphoblastic leukemia after chemotherapy, and bone marrow suppression and concurrent infection have the greatest influence on the quality of life and the treatment effect of children.
Objective To analyse the characteristics of adverse reactions in children with acute lymphoblastic leukemia after chemotherapy, and to implement individualized medication to avoid or reduce the occurrence of serious adrs. Methods The report on the adverse reactions in children with acute lymphoblastic leukemia after chemotherapy from 2015.2 to 2014.3 was collected, the patient gender, age, risk, drug and ADR involving organs or systems were analyzed. Results The adverse reactions in children with acute lymphoblastic leukemia after chemotherapy are involved mostly in the high-risk group of patients. L-asparaginase, vincristine and cytarabine were the top three drugs involved in adverse reactions. Blood system was the most common system involved in adverse reactions. Bone marrow suppression concurrent infection had significant difference between different risks and ages (P < 0.01) had no statistical difference between different genders (P > 0.05) . Conclusion Bone marrow suppression was the most common ADR in children with acute lymphoblastic leukemia after chemotherapy, and bone marrow suppression and concurrent infection have the greatest influence on the quality of life and the treatment effect of children.
2017, 38(10): 112-116.
Abstract:
Objective To discuss the clinical efficacy and safety of an standard dosage of decitabine in the treatment of patients with myelodysplastic syndromes. Methods Totally 37 cases of MDS patients received the treatment of standard dose of decitabine, (20 mg/m2 each day, 5 days continued, each four weeks is a course of treatment) , which admitted in Hematology department of the First Affiliated Hospital of Kunming Medical University from January 2014 to December 2016. According to the established therapeutic regimen, collecting treatment effects and safety parameters of patients, and according to different groups factors to analyze the difference of effects. Results Twenty patients (54.1%) obtained the clinical effect after the treatment of decitabineafter at least 4 courses. There were 3 cases (8.10%) with complete remission (CR) , marrow CR (m CR) without hematological improvement (HI) in 6 cases (16.2%) , m CR with HI in 3 cases (8.1%) , and HI alone in 8 cases (21.6%) ; 3 cases (8.1%) achieved transfusion independence, and 11 cases (29.7%) with stable disease (SD) and 3 cases (8.1%) with progressive disease (PD) . The total response rate of MDS was calculated according to the patient age, WHO type and prognosis risk stratification, decitabine treatment of 65 patients under the age of ORR above, more than 65 patients of/RAEB RAEB-1-2 type ORR is higher than other types of patient, but the difference was not statistically significant (The 2 values were 0.815 and 1.213, P>0.05) . The ORR of patients with high risk group of IPSS prognostic risk score was significantly better than that of low-risk group, the difference was statistically significant (χ2=4.063, P=0.044) .The ORR of the high-risk group of WPSS prognosis was significantly better than that of the lower risk group, the difference was statistically significant (χ2=4.375, P=0.036) .Adverse reactions were most commonly seen in Blood platelet count decreased, white blood cell count decreased and medium granulocyte count decreased. Thehighest incidence of platelet count decreased, white blood cell count decreased and medium granulocyte count decreased that was found in 3 ~ 4 adverse reactions. The incidence rate was 65.5%, 59.3% and 53.8% respectively. Non-hematological adverse reactions mainly include infection, ALT, diarrhea and rashes, and most grades are given priority to 1 ~ 2 level. Conclusion Decitabine for treatment of MDS is well tolerated, and the adverse reactions occur reasonably and controllable, it can achieve the expected curative effect.
Objective To discuss the clinical efficacy and safety of an standard dosage of decitabine in the treatment of patients with myelodysplastic syndromes. Methods Totally 37 cases of MDS patients received the treatment of standard dose of decitabine, (20 mg/m2 each day, 5 days continued, each four weeks is a course of treatment) , which admitted in Hematology department of the First Affiliated Hospital of Kunming Medical University from January 2014 to December 2016. According to the established therapeutic regimen, collecting treatment effects and safety parameters of patients, and according to different groups factors to analyze the difference of effects. Results Twenty patients (54.1%) obtained the clinical effect after the treatment of decitabineafter at least 4 courses. There were 3 cases (8.10%) with complete remission (CR) , marrow CR (m CR) without hematological improvement (HI) in 6 cases (16.2%) , m CR with HI in 3 cases (8.1%) , and HI alone in 8 cases (21.6%) ; 3 cases (8.1%) achieved transfusion independence, and 11 cases (29.7%) with stable disease (SD) and 3 cases (8.1%) with progressive disease (PD) . The total response rate of MDS was calculated according to the patient age, WHO type and prognosis risk stratification, decitabine treatment of 65 patients under the age of ORR above, more than 65 patients of/RAEB RAEB-1-2 type ORR is higher than other types of patient, but the difference was not statistically significant (The 2 values were 0.815 and 1.213, P>0.05) . The ORR of patients with high risk group of IPSS prognostic risk score was significantly better than that of low-risk group, the difference was statistically significant (χ2=4.063, P=0.044) .The ORR of the high-risk group of WPSS prognosis was significantly better than that of the lower risk group, the difference was statistically significant (χ2=4.375, P=0.036) .Adverse reactions were most commonly seen in Blood platelet count decreased, white blood cell count decreased and medium granulocyte count decreased. Thehighest incidence of platelet count decreased, white blood cell count decreased and medium granulocyte count decreased that was found in 3 ~ 4 adverse reactions. The incidence rate was 65.5%, 59.3% and 53.8% respectively. Non-hematological adverse reactions mainly include infection, ALT, diarrhea and rashes, and most grades are given priority to 1 ~ 2 level. Conclusion Decitabine for treatment of MDS is well tolerated, and the adverse reactions occur reasonably and controllable, it can achieve the expected curative effect.
2017, 38(10): 117-119.
Abstract:
Objective To discuss the treatment effect of amlodipine atorvastatin calcium tablets on hypertensive patients with coronary heart disease. Methods Sixty patients with hypertension combined coronary heart disease were randomly divided into two groups. The patients received the amlodipine atorvastatin calcium treatment were as the experimental group, the patients received nifedipine zyban treatment were as the control group, then we compared the effect of two groups.Results The total efficiency of the control group was lower than the experimental group, and the difference was statistically significant (P < 0.05) .The adverse reactions in the experimental group were less than the control group, and the difference was not statistically significant (P > 0.05) .Conclusion Amlodipine atorvastatin calcium tablet is simple and effective in the treatment of patients with hypertension combined with coronary heart disease, and has great value in clinic.
Objective To discuss the treatment effect of amlodipine atorvastatin calcium tablets on hypertensive patients with coronary heart disease. Methods Sixty patients with hypertension combined coronary heart disease were randomly divided into two groups. The patients received the amlodipine atorvastatin calcium treatment were as the experimental group, the patients received nifedipine zyban treatment were as the control group, then we compared the effect of two groups.Results The total efficiency of the control group was lower than the experimental group, and the difference was statistically significant (P < 0.05) .The adverse reactions in the experimental group were less than the control group, and the difference was not statistically significant (P > 0.05) .Conclusion Amlodipine atorvastatin calcium tablet is simple and effective in the treatment of patients with hypertension combined with coronary heart disease, and has great value in clinic.
2017, 38(10): 120-123.
Abstract:
Objective To analyze the influence of different pretreated methods on cleaning efficacy of surgical instruments polluted by hepatitis B virus (HBV) .Methods 600 HBV-contaminated surgical instruments were selected and divided randomly into 4 groups of pretreatment methods A, B, C and D, after pretreatment, four groups of instruments were washed in the verified automatic cleaning sterilizer. After washing, instruments were washed with pure water, the samples were taken, and the cleanliness of the instruments was inspected visually and by 5-time magnifier that has light source; the cleanliness was test by 3 M ATP fluorescence method; the positive rate of HBs Ag was detected by Quantitative ELISA.Results The visual pass rate of the cleanliness of 600 HBV-contaminated surgical instruments after four types of pretreatment was 100.00%, 82.67%, 100.00% and 90.67% respectively; which was 100.00%, 78.67%, 97.33% and 85.33% by 5-time magnifier that has light source; which was 98.67%, 72.67%, 96.00% and 80.00% by 3 M ATP fluorescence method;With regard to the above three test results, Group A was compared with Group B、Group D and Group B was compared with Group C while the differences were statistically significant (P<0.01) .The positive rate of HBs Ag detected by ELISA was 0. Conclusion Multiple Enzyme Detergent solution (1:270) can significantly improve the cleaning quality of HBV-contaminated surgical instruments, and reduce instruments loss.
Objective To analyze the influence of different pretreated methods on cleaning efficacy of surgical instruments polluted by hepatitis B virus (HBV) .Methods 600 HBV-contaminated surgical instruments were selected and divided randomly into 4 groups of pretreatment methods A, B, C and D, after pretreatment, four groups of instruments were washed in the verified automatic cleaning sterilizer. After washing, instruments were washed with pure water, the samples were taken, and the cleanliness of the instruments was inspected visually and by 5-time magnifier that has light source; the cleanliness was test by 3 M ATP fluorescence method; the positive rate of HBs Ag was detected by Quantitative ELISA.Results The visual pass rate of the cleanliness of 600 HBV-contaminated surgical instruments after four types of pretreatment was 100.00%, 82.67%, 100.00% and 90.67% respectively; which was 100.00%, 78.67%, 97.33% and 85.33% by 5-time magnifier that has light source; which was 98.67%, 72.67%, 96.00% and 80.00% by 3 M ATP fluorescence method;With regard to the above three test results, Group A was compared with Group B、Group D and Group B was compared with Group C while the differences were statistically significant (P<0.01) .The positive rate of HBs Ag detected by ELISA was 0. Conclusion Multiple Enzyme Detergent solution (1:270) can significantly improve the cleaning quality of HBV-contaminated surgical instruments, and reduce instruments loss.
2017, 38(10): 124-129.
Abstract:
To summarize and analyse the research condition, progress and area of studies on Dendrobium nobile by investigating domestic and foreign literatures about chemical constitutes and pharmacological effects from2010 to 2017. Dendrobium nobile Lindl is a sort of medicinal plant which mainly contains alkaloids, sesquiterpene, binenzyls, fluorenone, phenolic acid, lignin, phenylpropanoid, phenanthrenes, polysaccharide etc. Recently, the researches of Dendrobium nobile is mainly concentrated on chemical compounds, pharmacological effect and the cultivation techniques. In the forthcoming researches, utmost importance may be attached to the development and utilization of the relevant preparations, cosmetics as well as health care products.
To summarize and analyse the research condition, progress and area of studies on Dendrobium nobile by investigating domestic and foreign literatures about chemical constitutes and pharmacological effects from2010 to 2017. Dendrobium nobile Lindl is a sort of medicinal plant which mainly contains alkaloids, sesquiterpene, binenzyls, fluorenone, phenolic acid, lignin, phenylpropanoid, phenanthrenes, polysaccharide etc. Recently, the researches of Dendrobium nobile is mainly concentrated on chemical compounds, pharmacological effect and the cultivation techniques. In the forthcoming researches, utmost importance may be attached to the development and utilization of the relevant preparations, cosmetics as well as health care products.
2017, 38(10): 130-133.
Abstract:
Objective To study the opinions about resident standardization training in clinical medical students of Kunming Medical University and improve the teaching quality. Method This study adopted random sampling method and investigated medical students using a self-designed questionnaire, SPSS17.0 data analysis software was used to analyze the results.Result The results suggested that most of the students were expected to improve the ability of medical practice and doctor-patient communication skills;part of the students worried about the salary, the uneven level of teaching doctors and so on. Conclusion The school should establish effective policy propaganda and communication platform, to strengthen publicity, government at all levels and departments should try their best to improve the students' treatment and the teaching management system, strengthen the teacher training and so on, then get the strong support on the policy from medical students.
Objective To study the opinions about resident standardization training in clinical medical students of Kunming Medical University and improve the teaching quality. Method This study adopted random sampling method and investigated medical students using a self-designed questionnaire, SPSS17.0 data analysis software was used to analyze the results.Result The results suggested that most of the students were expected to improve the ability of medical practice and doctor-patient communication skills;part of the students worried about the salary, the uneven level of teaching doctors and so on. Conclusion The school should establish effective policy propaganda and communication platform, to strengthen publicity, government at all levels and departments should try their best to improve the students' treatment and the teaching management system, strengthen the teacher training and so on, then get the strong support on the policy from medical students.
2017, 38(10): 134-140.
Abstract:
Objectives To explore how Ph D students' learning needs and motivation of interpreting are correlated with autonomous learning time, furthermore, how to facilitate their interpreting learning and optimize interpretation teaching in Kunming Medical University. Methods By cluster sampling, 122 Ph D students in Grades2015 and 2016 were chosen as objects with a questionnaire in this research. Results The correlation analysis reveals there is no correlation between interpreting learning needs and autonomous learning time (rs=0.041, P=0.693) . While the correlation analysis on interpreting learning motivation and autonomous learning time indicates the positive correlation (rs=0.248, P=0.016) . Conclusion There is a close relationship between learning motivation and autonomous learning. The level of learning motivation determines the ability of autonomous learning, whereas autonomous learning promotes and enhances students' learning motivation. In teaching interpretation, students' learning needs have to be regarded as the core element in the curriculum design, strengthening English language proficiency ought to be the teaching aim, course content and teaching approach need to be further optimized, and more interpretation practice should be assigned to students.
Objectives To explore how Ph D students' learning needs and motivation of interpreting are correlated with autonomous learning time, furthermore, how to facilitate their interpreting learning and optimize interpretation teaching in Kunming Medical University. Methods By cluster sampling, 122 Ph D students in Grades2015 and 2016 were chosen as objects with a questionnaire in this research. Results The correlation analysis reveals there is no correlation between interpreting learning needs and autonomous learning time (rs=0.041, P=0.693) . While the correlation analysis on interpreting learning motivation and autonomous learning time indicates the positive correlation (rs=0.248, P=0.016) . Conclusion There is a close relationship between learning motivation and autonomous learning. The level of learning motivation determines the ability of autonomous learning, whereas autonomous learning promotes and enhances students' learning motivation. In teaching interpretation, students' learning needs have to be regarded as the core element in the curriculum design, strengthening English language proficiency ought to be the teaching aim, course content and teaching approach need to be further optimized, and more interpretation practice should be assigned to students.
2017, 38(10): 141-144.
Abstract:
Objective To discuss the effect QCC in reducing the unplanned extubation rate of nasogastric tube, nursing cost in Department of Neurology and improve the quality of nursing at the same time.Methods We randomly selected the Department of Neurology in the fourth affiliated hospital of Kunming Medical University.Before QCC management, there are indwelling nasogastric tube 50 cases as control group on July to August, 2015.After QCC management, there are indwelling nasogastric tube 51 cases as experimental group on December 2015 to February 2016.In the strategy step, We add evidence based nursing method, making the literature search strategy closely, chose nasal bridles.The contents of the survey include: The rate of unplanned extubation of nasogastric tube, working hours in indwelling nasogastric tube, nursing cost before and after the QCC management mode in Department of Neurology.Statistical analysis of data using SPSS17.0 statistical software.Result The two groups had no significant difference in age, gender, disease (P>0.05) . Compared with the two groups, the unplanned extubation rate decreased from 62% to 17.65%, the cost of materials decreased from (11.81 ± 9.08) yuan/person to 7.30 ± 2.53 yuan/person (P<0.05) , the working hours of nasogastric tube placement decreased from (13.22±10.77) minutes/person to (7.48±2.56) minutes/person (P<0.05) . The differences were statistically significant (P < 0.05) .Conclusions The QCC mode can reduce unplanned extubation rate of neurological nasogastric tube, reduce the cost of care and improve the quality of patient care in the Department of Neurology.
Objective To discuss the effect QCC in reducing the unplanned extubation rate of nasogastric tube, nursing cost in Department of Neurology and improve the quality of nursing at the same time.Methods We randomly selected the Department of Neurology in the fourth affiliated hospital of Kunming Medical University.Before QCC management, there are indwelling nasogastric tube 50 cases as control group on July to August, 2015.After QCC management, there are indwelling nasogastric tube 51 cases as experimental group on December 2015 to February 2016.In the strategy step, We add evidence based nursing method, making the literature search strategy closely, chose nasal bridles.The contents of the survey include: The rate of unplanned extubation of nasogastric tube, working hours in indwelling nasogastric tube, nursing cost before and after the QCC management mode in Department of Neurology.Statistical analysis of data using SPSS17.0 statistical software.Result The two groups had no significant difference in age, gender, disease (P>0.05) . Compared with the two groups, the unplanned extubation rate decreased from 62% to 17.65%, the cost of materials decreased from (11.81 ± 9.08) yuan/person to 7.30 ± 2.53 yuan/person (P<0.05) , the working hours of nasogastric tube placement decreased from (13.22±10.77) minutes/person to (7.48±2.56) minutes/person (P<0.05) . The differences were statistically significant (P < 0.05) .Conclusions The QCC mode can reduce unplanned extubation rate of neurological nasogastric tube, reduce the cost of care and improve the quality of patient care in the Department of Neurology.
2017, 38(10): 145-148.
Abstract:
Objective To explore the effect of cognitive level of intervening nursing care on brain function recovery, state of consciousness and awakening in patients with craniocerebral injury combined with severe cerebral coma. Methods From January 2014 to November 2016, 84 patients with ICU craniocerebral injury complicated with severe coma were randomly divided into two groups: 42 cases. The control group was treated by routine nursing group, and the observation group was informed of the level of intervening nursing care, and the brain function recovery, consciousness state and awakening were compared between the two groups. Results The scores of the observation group were higher than those of the control group, the difference was statistically significant (P <0.05) . The survival rate of the observation group was higher than that of the control group, the difference was statistically significant (P<0.05) . The recovery rate of the awakening time in the observation group was higher than that in the control group at 1-10 days (P<0.05) . Conclusions The introduction of cognitive level of intervening care into ICU craniocerebral injury with severe cerebral coma is effective, which can improve the recovery of brain function and improve the state of consciousness and awakening. It is worth popularizing.
Objective To explore the effect of cognitive level of intervening nursing care on brain function recovery, state of consciousness and awakening in patients with craniocerebral injury combined with severe cerebral coma. Methods From January 2014 to November 2016, 84 patients with ICU craniocerebral injury complicated with severe coma were randomly divided into two groups: 42 cases. The control group was treated by routine nursing group, and the observation group was informed of the level of intervening nursing care, and the brain function recovery, consciousness state and awakening were compared between the two groups. Results The scores of the observation group were higher than those of the control group, the difference was statistically significant (P <0.05) . The survival rate of the observation group was higher than that of the control group, the difference was statistically significant (P<0.05) . The recovery rate of the awakening time in the observation group was higher than that in the control group at 1-10 days (P<0.05) . Conclusions The introduction of cognitive level of intervening care into ICU craniocerebral injury with severe cerebral coma is effective, which can improve the recovery of brain function and improve the state of consciousness and awakening. It is worth popularizing.
