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RSS2017 Vol. 38, No. 06
2017, 38(06): 1-4.
Abstract:
Objective To construct a recombinant vector containing human WDR5 and establish LNCaP cell strain with stable expression of WDR5. Me thods WDR5 gene was amplified by PCR and was cloned into eukaryotic expression vector pCI-neo. The resulted pCI-neo-W...
Objective To construct a recombinant vector containing human WDR5 and establish LNCaP cell strain with stable expression of WDR5. Me thods WDR5 gene was amplified by PCR and was cloned into eukaryotic expression vector pCI-neo. The resulted pCI-neo-W...
2017, 38(06): 5-8.
Abstract:
目的 构建神经生长因子 (nerve growth factor, NGF) 基因-骨髓基质干细胞 (bone marrow stromal stem cells, BMSCs) 重组体, 将其移植入阿尔茨海默病 (alzheimer's disease, AD) 大鼠海马, 探讨AD大鼠神经行为学变化.方法 将Aβ淀粉蛋白 (β-amyloid protein) 立体定向注射入大鼠海马制备AD模型.动物随机分为5组:正常组 (Normal group) 、盐水注射组 (NS group) 、模...
2017, 38(06): 9-14.
Abstract:
Objective To investigate the influence of bone marrow stem cell transplantation on immunosuppressed mice induced by radiotherapy. Me thods Female BALB/C mice were randomly divided into three groups. The mice in test group and in control group were ir...
Objective To investigate the influence of bone marrow stem cell transplantation on immunosuppressed mice induced by radiotherapy. Me thods Female BALB/C mice were randomly divided into three groups. The mice in test group and in control group were ir...
2017, 38(06): 15-18.
Abstract:
Objective To build animal model of hyperhomocysteinemia (HHcy) , and to investigate the expression of TRB3 in adipose tissue and explore the effect of PI3K/Akt signaling pathway by TRB3.Me thods Twenty mice were divided into the fasting control group...
Objective To build animal model of hyperhomocysteinemia (HHcy) , and to investigate the expression of TRB3 in adipose tissue and explore the effect of PI3K/Akt signaling pathway by TRB3.Me thods Twenty mice were divided into the fasting control group...
2017, 38(06): 19-24.
Abstract:
Objective To investigate a safe and effective clinical-grade cryoprotectant, which can be infused to patients. Me thods We collected 50 samples of cord blood using hydroxyethyl starch precipitation method to get cord blood nucleated cells. Then we ad...
Objective To investigate a safe and effective clinical-grade cryoprotectant, which can be infused to patients. Me thods We collected 50 samples of cord blood using hydroxyethyl starch precipitation method to get cord blood nucleated cells. Then we ad...
2017, 38(06): 25-27.
Abstract:
Objective To explore the basic situation of the quality of life of rural elderly people of different gender WA, provide the basis for improving the quality of life of ethnic minority elderly. Me thods SF-36 scale was used to measure the quality of li...
Objective To explore the basic situation of the quality of life of rural elderly people of different gender WA, provide the basis for improving the quality of life of ethnic minority elderly. Me thods SF-36 scale was used to measure the quality of li...
2017, 38(06): 28-31.
Abstract:
Objective To investigate the clinical significance of Tiam1 and Fascin protein expression in esophagus squamous carcinoma.Me thods Immunohistochemical SP method was used to detect Tiam1, Fascin protein in esophageal squamous carcinoma and normal esop...
Objective To investigate the clinical significance of Tiam1 and Fascin protein expression in esophagus squamous carcinoma.Me thods Immunohistochemical SP method was used to detect Tiam1, Fascin protein in esophageal squamous carcinoma and normal esop...
2017, 38(06): 32-35.
Abstract:
Objective To investigate the correlation between the age of onset and acute coronary syndrome (ACS) . Methods A total of 300 patients with ACS diagnosed in our hospital from June 2014 to June 2016 were selected in this study. Their age, history of sm...
Objective To investigate the correlation between the age of onset and acute coronary syndrome (ACS) . Methods A total of 300 patients with ACS diagnosed in our hospital from June 2014 to June 2016 were selected in this study. Their age, history of sm...
2017, 38(06): 36-39.
Abstract:
目的 探讨不同类型的非生物型人工肝技术对肝衰竭合并肝肾综合征的临床疗效.方法 选取2014年1月至2015年12月在昆明市第三人民医院住院的肝功能衰竭合并肝肾综合征患者60例作为研究对象, 共行人工肝治疗317人次.入院后根据治疗方案的不同分为观察组和对照组, 每组30例, 观察组给予血浆置换联合血液透析滤过 (plasma exchange+hemo-diafiltration, PE+HDF) 治疗, 对照组给予血浆置换 (plasma exchange, PE) 治疗.观察2组治疗后肝肾功...
2017, 38(06): 40-43.
Abstract:
Objective To investigate the incidence of nosocomial infection in ICU, mortality rate of infected patients, the risk-factors and strategies of nosocomial infection. Me thods We retrospectively analyzed all ICU patients' medical records from August 20...
Objective To investigate the incidence of nosocomial infection in ICU, mortality rate of infected patients, the risk-factors and strategies of nosocomial infection. Me thods We retrospectively analyzed all ICU patients' medical records from August 20...
2017, 38(06): 44-47.
Abstract:
Objective To explore the clinical efficacy of Ilizarov technique in treatment of Tibial infected bone defect, and analyze the best indications. Me thods We retrospectively analysed the data of 15 cases of tibial infected bone defect, from January 201...
Objective To explore the clinical efficacy of Ilizarov technique in treatment of Tibial infected bone defect, and analyze the best indications. Me thods We retrospectively analysed the data of 15 cases of tibial infected bone defect, from January 201...
2017, 38(06): 48-51.
Abstract:
Objective To evaluate diagnostic significance of single high frequency ultrasound and mammography and combined therapy of both on breast cancer. Me thods 355 cases of female breast cancer were selected from The First Hospital of Kunming from December...
Objective To evaluate diagnostic significance of single high frequency ultrasound and mammography and combined therapy of both on breast cancer. Me thods 355 cases of female breast cancer were selected from The First Hospital of Kunming from December...
2017, 38(06): 52-55.
Abstract:
Objective To establish an automatic typing method for MAOA μ-VNTR polymorphism test using capillary electrophoresis and fluorescence labeling techniques. Me thods DNAwas extracted by Chelex-100 method. Blood samples from 720 unrelated Chinese Han ind...
Objective To establish an automatic typing method for MAOA μ-VNTR polymorphism test using capillary electrophoresis and fluorescence labeling techniques. Me thods DNAwas extracted by Chelex-100 method. Blood samples from 720 unrelated Chinese Han ind...
2017, 38(06): 56-59.
Abstract:
Objective To study the dust mites species and predominant species in Kunming, to provide evidence for the prevention and cure of dust mite allergy. Me thods From August 2010 to August 2011, mite-allergic patients, including allergic rhinitis and asth...
Objective To study the dust mites species and predominant species in Kunming, to provide evidence for the prevention and cure of dust mite allergy. Me thods From August 2010 to August 2011, mite-allergic patients, including allergic rhinitis and asth...
2017, 38(06): 60-63.
Abstract:
目的 探讨武汉地区冠心病患者药物代谢酶CYP2C19基因多态性的分布.方法 采用基因芯片法对123例服用氯吡格雷的无血缘关系的冠心病患者进行CYP2C19基因分型, 分析其基因型及代谢表型的分布, 并与文献报道中中国汉族人群进行比较.结果 共统计发现5种基因型, 3种代谢表型;检测出快代谢型 (EM) 患者38例, 所占比例为30.9%, 检测出中间代谢型 (IM) 患者69例, 所占比例为56.1%, 检测出慢代谢型 (PM) 患者16例, 所占比例为13.0%.武汉地区冠心病患者中, CYP...
2017, 38(06): 64-67.
Abstract:
Objective To study the changes in the composition of main lymphocytes in peripheral blood of patients with AECOPD (acute exacerbation of chronic obstructive pulmonary disease) . Me thods From January1 th, 2014 to 31 th December, total 789 admitted pa...
Objective To study the changes in the composition of main lymphocytes in peripheral blood of patients with AECOPD (acute exacerbation of chronic obstructive pulmonary disease) . Me thods From January1 th, 2014 to 31 th December, total 789 admitted pa...
2017, 38(06): 68-72.
Abstract:
Objective To investigate the risk factors of myocardial infarction in Han population from Yunnan province. Me thods 500 myocardial infarction patients and 350 non CHD patients were selected in the affiliated Yanan Hospital of Kunming Medical Universi...
Objective To investigate the risk factors of myocardial infarction in Han population from Yunnan province. Me thods 500 myocardial infarction patients and 350 non CHD patients were selected in the affiliated Yanan Hospital of Kunming Medical Universi...
2017, 38(06): 73-76.
Abstract:
Objective To investigate the effect of pulmonary dead-space fraction on the prognosis of acute respiratory distress syndrome (ARDS) . Me thods 32 patients meeting ARDS Berlin definition, who were admitted to the EICU from January 2015 to August 2016,...
Objective To investigate the effect of pulmonary dead-space fraction on the prognosis of acute respiratory distress syndrome (ARDS) . Me thods 32 patients meeting ARDS Berlin definition, who were admitted to the EICU from January 2015 to August 2016,...
2017, 38(06): 77-81.
Abstract:
Objective To evaluate the short-term curative effect of PKRP and Tm LRP for benign prostatic hyperplasia (BPH) . Me thods We selected 100 subjects who would like to accept surgical treatment, and then randomly divided them into two groups (PKRP and T...
Objective To evaluate the short-term curative effect of PKRP and Tm LRP for benign prostatic hyperplasia (BPH) . Me thods We selected 100 subjects who would like to accept surgical treatment, and then randomly divided them into two groups (PKRP and T...
2017, 38(06): 82-86.
Abstract:
Objective To investigate the diagnostic value and correlation of corresponding laboratory indexes in rheumatoid arthritis (RA) complicated with coronary heart disease (CHD) . Me thods According to gender and age, patients were divided into RA concurr...
Objective To investigate the diagnostic value and correlation of corresponding laboratory indexes in rheumatoid arthritis (RA) complicated with coronary heart disease (CHD) . Me thods According to gender and age, patients were divided into RA concurr...
2017, 38(06): 87-91.
Abstract:
Objective To observe the analgesic effect of ultrasound-guided paravertebral nerve block on analgesia after thoracotomy. Me thods 70 patients who were in ASA Ⅰ ~ Ⅱ and planned to undergo elective thoracotomy surgery with tracheal intubation anesthesi...
Objective To observe the analgesic effect of ultrasound-guided paravertebral nerve block on analgesia after thoracotomy. Me thods 70 patients who were in ASA Ⅰ ~ Ⅱ and planned to undergo elective thoracotomy surgery with tracheal intubation anesthesi...
2017, 38(06): 92-96.
Abstract:
Objective To explore the relationship between acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and pulmonary hypertension (PH) in patients with chronic obstructive pulmonary disease (COPD) . Me thods Using retrospective analysis, ...
Objective To explore the relationship between acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and pulmonary hypertension (PH) in patients with chronic obstructive pulmonary disease (COPD) . Me thods Using retrospective analysis, ...
2017, 38(06): 97-101.
Abstract:
Objective To analyze the differentially expressed genes and signaling pathways in cisplatin resistant ovarian cancer. Me thods Cisplatin resistant ovarian cancer gene set GSE15372 was obtained from the GEO database of the national information center ...
Objective To analyze the differentially expressed genes and signaling pathways in cisplatin resistant ovarian cancer. Me thods Cisplatin resistant ovarian cancer gene set GSE15372 was obtained from the GEO database of the national information center ...
2017, 38(06): 102-107.
Abstract:
Objective To monitor placental function by using the ultrasonic blood flow index and serumβ-HCG.Me thods From May 2012 to December 2015 in our hospital, 1053 cases of prenatal puerperas were given placenta vascularization index (VI) , blood flow inde...
Objective To monitor placental function by using the ultrasonic blood flow index and serumβ-HCG.Me thods From May 2012 to December 2015 in our hospital, 1053 cases of prenatal puerperas were given placenta vascularization index (VI) , blood flow inde...
2017, 38(06): 108-112.
Abstract:
Objective To explore the influence of HSF2 on occurrence of lung cancer and tumor cell proliferation by promoting HSP's expression. Me thods We selected the para-carcinoma tissue from patients with lung cancer, then constructed the eukaryoticvector o...
Objective To explore the influence of HSF2 on occurrence of lung cancer and tumor cell proliferation by promoting HSP's expression. Me thods We selected the para-carcinoma tissue from patients with lung cancer, then constructed the eukaryoticvector o...
2017, 38(06): 113-117.
Abstract:
Objective To analyze the early clinical outcome of Zero-P VA during the operation of anterior cervical discectomy and fusion ( ACDF) in the treatment of cervical spondylotic myelopathy retrospectively.Me thods A total of 29 patients (33 Zero-P VA has...
Objective To analyze the early clinical outcome of Zero-P VA during the operation of anterior cervical discectomy and fusion ( ACDF) in the treatment of cervical spondylotic myelopathy retrospectively.Me thods A total of 29 patients (33 Zero-P VA has...
2017, 38(06): 118-123.
Abstract:
Objective To evaluate the clinical efficacy and adverse effects induced by rh-endostatin (rh-ES) combined with chemotherapy in the treatment of advanced breast cancer. Methods The Pubmed, Embase, Cochrane Library, Spriner, CNKI, VIP, Wangfang Data, C...
Objective To evaluate the clinical efficacy and adverse effects induced by rh-endostatin (rh-ES) combined with chemotherapy in the treatment of advanced breast cancer. Methods The Pubmed, Embase, Cochrane Library, Spriner, CNKI, VIP, Wangfang Data, C...
2017, 38(06): 124-129.
Abstract:
Objective To explore the clinical application value of CT plus MRI in the staging diagnosis of endometrial cancer, and to evaluate the malignancy of cancer according to the postoperative level of VEGF-C.Me thods A total of 70 pathologically conformed...
Objective To explore the clinical application value of CT plus MRI in the staging diagnosis of endometrial cancer, and to evaluate the malignancy of cancer according to the postoperative level of VEGF-C.Me thods A total of 70 pathologically conformed...
2017, 38(06): 130-135.
Abstract:
Objective To compare the clinical application of atrial active leads and passive leads, and to investigate the feasibility and safety of atrial active electrode lead. Me thods 1 027 patients with pacemaker implantation in Yuxi People's Hospital from ...
Objective To compare the clinical application of atrial active leads and passive leads, and to investigate the feasibility and safety of atrial active electrode lead. Me thods 1 027 patients with pacemaker implantation in Yuxi People's Hospital from ...
2017, 38(06): 136-139.
Abstract:
Objective To investigate the correlation between liver tissue inflammation and fibrosis degrees and CD4+ T-lymphocytes level in the patients with HCV/HIV co-infection. Me thods The relationship between liver biopsy histological inflammatory grade and...
Objective To investigate the correlation between liver tissue inflammation and fibrosis degrees and CD4+ T-lymphocytes level in the patients with HCV/HIV co-infection. Me thods The relationship between liver biopsy histological inflammatory grade and...
2017, 38(06): 140-143.
Abstract:
Nursing quality is closely related to the curative effect, treatment compliance, treatment cost, quality of life and recurrence rate of patients with peptic ulcer. In order to summarize the recent progress in the research and practice of peptic ulcer...
Nursing quality is closely related to the curative effect, treatment compliance, treatment cost, quality of life and recurrence rate of patients with peptic ulcer. In order to summarize the recent progress in the research and practice of peptic ulcer...
2017, 38(06): 144-146.
Abstract:
Objective In order to adapt to the requirements of teaching reform and to stimulate students' interest, Combination of PBL, CBL and TBL teaching method is introduced to the anesthetic pharmacology experiment, and its teaching effect is discussed. Me ...
Objective In order to adapt to the requirements of teaching reform and to stimulate students' interest, Combination of PBL, CBL and TBL teaching method is introduced to the anesthetic pharmacology experiment, and its teaching effect is discussed. Me ...
2017, 38(06): 147-150.
Abstract:
Objective To explore the application effect of U fixation in neonatal needle indwelling. Methods318 cases of newborns with needle indwelling for intravenous infusion in the department of pediatrics in the third people's hospital in Yunnan Province in...
Objective To explore the application effect of U fixation in neonatal needle indwelling. Methods318 cases of newborns with needle indwelling for intravenous infusion in the department of pediatrics in the third people's hospital in Yunnan Province in...
