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RSS2018 Vol. 39, No. 04
2018, 39(04): 1-4.
Abstract:
Objective To construct GST-SRY fusion protein expression vector and induce its expression in E.coli, then purificate so as to obtain GST-SRY fusion protein. Me thods SRY gene was amplified by PCR and cloned into prokaryotic expression vector p GEX-6 ...
Objective To construct GST-SRY fusion protein expression vector and induce its expression in E.coli, then purificate so as to obtain GST-SRY fusion protein. Me thods SRY gene was amplified by PCR and cloned into prokaryotic expression vector p GEX-6 ...
2018, 39(04): 5-10.
Abstract:
Objective To explore the effect of Edaravone (EDA) on oxidative stress such as release of nitric oxide (NO) induced by Lipopolysaccharide (LPS) in activation of primary microglia. Me thods There were four groups including control, LPS, LPS + EDA and ...
Objective To explore the effect of Edaravone (EDA) on oxidative stress such as release of nitric oxide (NO) induced by Lipopolysaccharide (LPS) in activation of primary microglia. Me thods There were four groups including control, LPS, LPS + EDA and ...
2018, 39(04): 11-15.
Abstract:
Objective To investigate the regulation of scutellarin (SCU) on protein and m RNA expression of PKCε in Human cardiac microvascular endothelial cells (HCMECs) induced by hypoxia reoxygenation or normal. Me thods We cultured HCMECs in two ways: normal...
Objective To investigate the regulation of scutellarin (SCU) on protein and m RNA expression of PKCε in Human cardiac microvascular endothelial cells (HCMECs) induced by hypoxia reoxygenation or normal. Me thods We cultured HCMECs in two ways: normal...
2018, 39(04): 16-20.
Abstract:
Object To detect the effect of terlipressin and FK-409 on protection of liver graft injury after reduced-size liver transplantation in rats. Me thods We performed liver transplantation using 30% volume liver graft in rats. ALT, total bilirubin, porta...
Object To detect the effect of terlipressin and FK-409 on protection of liver graft injury after reduced-size liver transplantation in rats. Me thods We performed liver transplantation using 30% volume liver graft in rats. ALT, total bilirubin, porta...
2018, 39(04): 21-26.
Abstract:
Objective To explore the influence of CD151 inhibition by RNA interference on lung metastasis in null mice given tail veins injection of lung adenocarcinoma cell lines A549. METHODS: Constructed CD151 inhibition by RNA interference cell lines A549-sh...
Objective To explore the influence of CD151 inhibition by RNA interference on lung metastasis in null mice given tail veins injection of lung adenocarcinoma cell lines A549. METHODS: Constructed CD151 inhibition by RNA interference cell lines A549-sh...
2018, 39(04): 27-33.
Abstract:
Objective To establish animal model of non-alcoholic fatty liver disease (NAFLD) and explore the molecular mechanism of homocysteine changes in SD rats with NAFLD by ginsenoside Rg1 treating. Me thods NAFLD animal model was established to determine s...
Objective To establish animal model of non-alcoholic fatty liver disease (NAFLD) and explore the molecular mechanism of homocysteine changes in SD rats with NAFLD by ginsenoside Rg1 treating. Me thods NAFLD animal model was established to determine s...
2018, 39(04): 34-39.
Abstract:
Objective To establish an oxidative stress cell model by inducing human umbilical vein endothelial cells (HUVECs) using hydrogen peroxide (H2 O2) . Methods Six gradient concentrations of H2 O2 were co-cultured with HUVECs for 6 h, 12 h, 24 h and 48 h...
Objective To establish an oxidative stress cell model by inducing human umbilical vein endothelial cells (HUVECs) using hydrogen peroxide (H2 O2) . Methods Six gradient concentrations of H2 O2 were co-cultured with HUVECs for 6 h, 12 h, 24 h and 48 h...
2018, 39(04): 40-46.
Abstract:
Objective To identify two Fasciola spp., Sample YXGD01 and YXGD02, collected from one female fasciolosis patient living in Yuxin, Yunnan Province in China using DNA marker nad1 and ITS1, and to investigate causative agents of Zoonoses fasciolosis in ...
Objective To identify two Fasciola spp., Sample YXGD01 and YXGD02, collected from one female fasciolosis patient living in Yuxin, Yunnan Province in China using DNA marker nad1 and ITS1, and to investigate causative agents of Zoonoses fasciolosis in ...
2018, 39(04): 47-51.
Abstract:
Objective To investigate the association between single nucleotide polymorphisms (SNPs) in IL1 RN and risk of glioma. Me thods A total of 200 glioma patients and 200 healthy controls were enrolled in Kuangwu Hospital of Tongchuan from January 2012 to...
Objective To investigate the association between single nucleotide polymorphisms (SNPs) in IL1 RN and risk of glioma. Me thods A total of 200 glioma patients and 200 healthy controls were enrolled in Kuangwu Hospital of Tongchuan from January 2012 to...
2018, 39(04): 52-55.
Abstract:
Objective To investigate the effect of cinnamaldehyde and vancomycin on Biofilm inhibition of methicillin-resistant staphylococcus aureus (MRSA) . Me thods Modified plate culture method was used to establish in vitro biofilm model for MRSA standard s...
Objective To investigate the effect of cinnamaldehyde and vancomycin on Biofilm inhibition of methicillin-resistant staphylococcus aureus (MRSA) . Me thods Modified plate culture method was used to establish in vitro biofilm model for MRSA standard s...
2018, 39(04): 62-65.
Abstract:
Objective To investigate the value of contrast enhanced ultrasonography in catheter drainage to determine the position and patency of catheter. Me thods We made a retrospective analysis in patients (78 cases) received percutaneous ultrasound-guided c...
Objective To investigate the value of contrast enhanced ultrasonography in catheter drainage to determine the position and patency of catheter. Me thods We made a retrospective analysis in patients (78 cases) received percutaneous ultrasound-guided c...
2018, 39(04): 66-69.
Abstract:
Objective To explore the relationship between the mucosal inflammation and the surgical efficacy of patients with eosinophilic chronic sinusitis. Me thods From September 2014 to September 2017, 30 patients with chronic eosinophils sinusitis were sele...
Objective To explore the relationship between the mucosal inflammation and the surgical efficacy of patients with eosinophilic chronic sinusitis. Me thods From September 2014 to September 2017, 30 patients with chronic eosinophils sinusitis were sele...
2018, 39(04): 70-72.
Abstract:
Objective To study the incidence of pharyngeal fistula and the use of antibiotics in the patients with laryngeal cancer underwent total laryngectomy and to analyze the distribution of bacteria in the operative area of laryngectomy to provide some exp...
Objective To study the incidence of pharyngeal fistula and the use of antibiotics in the patients with laryngeal cancer underwent total laryngectomy and to analyze the distribution of bacteria in the operative area of laryngectomy to provide some exp...
2018, 39(04): 79-82.
Abstract:
Objective To investigate the differential diagnostic value of the Apparent Diffusion Coefficient in Interstitial Edema Pancreatitis (IEP) and Acute Necrotizing Pancreatitis (ANP) . Me thods Twenty-four ANP patients and 53 IEP patients received routin...
Objective To investigate the differential diagnostic value of the Apparent Diffusion Coefficient in Interstitial Edema Pancreatitis (IEP) and Acute Necrotizing Pancreatitis (ANP) . Me thods Twenty-four ANP patients and 53 IEP patients received routin...
2018, 39(04): 83-87.
Abstract:
Objective To analyze the efficacy and safety of different surgical procedures in the treatment of suppurative encapsulated pleural effusion. Me thods Sixty-eight patients with suppurative pleural effusion were randomly divided into group A and group ...
Objective To analyze the efficacy and safety of different surgical procedures in the treatment of suppurative encapsulated pleural effusion. Me thods Sixty-eight patients with suppurative pleural effusion were randomly divided into group A and group ...
2018, 39(04): 88-94.
Abstract:
Objective To investigate the association of Vitamin D receptor gene Apa I, Fok I, Taq I, Tru9 I single nucleotide polymorphism with hashimoto's thyroiditis (HT) . Me thods The single nucleotide polymorphism of VDR gene in 139 (HT group) and 66 normal...
Objective To investigate the association of Vitamin D receptor gene Apa I, Fok I, Taq I, Tru9 I single nucleotide polymorphism with hashimoto's thyroiditis (HT) . Me thods The single nucleotide polymorphism of VDR gene in 139 (HT group) and 66 normal...
2018, 39(04): 95-99.
Abstract:
Objective Through the comprehensive geriatric assessment, the incidence of anxiety and related risk factors of the elderly hypertensive patients in the general hospital are understood and the basis for clinical intervention is provided.Me thods We ra...
Objective Through the comprehensive geriatric assessment, the incidence of anxiety and related risk factors of the elderly hypertensive patients in the general hospital are understood and the basis for clinical intervention is provided.Me thods We ra...
2018, 39(04): 100-103.
Abstract:
Objective To observe the incidence of secondary epilepsy in patients with cerebral infarction and explore the risk factors of secondary epilepsy. Me thods Eighty-four patients with secondary epilepsy diagnosed as cerebral infarction from April 2016 t...
Objective To observe the incidence of secondary epilepsy in patients with cerebral infarction and explore the risk factors of secondary epilepsy. Me thods Eighty-four patients with secondary epilepsy diagnosed as cerebral infarction from April 2016 t...
2018, 39(04): 104-108.
Abstract:
目的 探究幽门螺杆菌 (H.pylori) 对不同种抗菌药物耐药问题的研究.方法 选取云南省昆明市第一人民医院2015年8月至2017年4月接诊出现消化道症状且经胃镜检查确诊幽门螺杆菌感染的240例患者为研究对象, 按照临床给药不同分为A、B、C 3组, A组80例患者给予 (埃索美拉唑+甲硝唑+左氧氟沙星) 治疗, B组80例患者给予 (埃索美拉唑+克拉霉素+左氧氟沙星) 治疗, C组80例患者给予 (埃索美拉唑+阿莫西林+左氧氟沙星) 治疗, 3组患者治疗10 d后通过胃镜从患者胃窦取2块胃...
2018, 39(04): 109-112.
Abstract:
Objective To evaluate the role of lung ultrasonography (LUS) in the diagnosis of neonatal pneumonia in plateau area. Me thods A total number of 200 cases of clinically diagnosed neonatal pneumonia were examined by bedside lung ultrasound from June 20...
Objective To evaluate the role of lung ultrasonography (LUS) in the diagnosis of neonatal pneumonia in plateau area. Me thods A total number of 200 cases of clinically diagnosed neonatal pneumonia were examined by bedside lung ultrasound from June 20...
2018, 39(04): 113-116.
Abstract:
Objective To summarize the characteristics of PPP disease complex with placenta implication and explore the value of prenatal ultrasonographic scoring system in placenta prevlia complicate with placenta accrete.Me thods An retrospective analysis was ...
Objective To summarize the characteristics of PPP disease complex with placenta implication and explore the value of prenatal ultrasonographic scoring system in placenta prevlia complicate with placenta accrete.Me thods An retrospective analysis was ...
2018, 39(04): 117-120.
Abstract:
Objective To investigate the effects of amniotic fluid meconium contamination and timely termination of pregnancy on perinatal outcome of intrahepatic cholestasis of pregnancy (ICP) . Me thods From December 2012 to December 2015, 208 cases of ICP wer...
Objective To investigate the effects of amniotic fluid meconium contamination and timely termination of pregnancy on perinatal outcome of intrahepatic cholestasis of pregnancy (ICP) . Me thods From December 2012 to December 2015, 208 cases of ICP wer...
2018, 39(04): 121-124.
Abstract:
Objective To investigate the cause of dropout among people living with HIV/AIDS receiving free antiretroviral therapy (ART) in Yunnan province and its associated factors. Me thods Data of patients with HIV/AIDS receiving ART were collected from ART i...
Objective To investigate the cause of dropout among people living with HIV/AIDS receiving free antiretroviral therapy (ART) in Yunnan province and its associated factors. Me thods Data of patients with HIV/AIDS receiving ART were collected from ART i...
2018, 39(04): 125-129.
Abstract:
Objective To explore the preventive measures through the analysis of the influential factors and the characteristics of human wounded based on the AIS (abbreviated injury scale) score to the occupant of the coach happened head-on collision. Me thods ...
Objective To explore the preventive measures through the analysis of the influential factors and the characteristics of human wounded based on the AIS (abbreviated injury scale) score to the occupant of the coach happened head-on collision. Me thods ...
2018, 39(04): 130-134.
Abstract:
糖尿病是临床常见慢性代谢性疾病, 是失明、肾衰竭、心脏病发作、中风和下肢截肢的主要病因, 死亡率逐年增加, 据预测, 2030年, 糖尿病将成为第7主要死因.因此, 糖尿病的预防及治疗至关重要.近年来, 国内外相关研究表明, 通过开展糖尿病健康教育, 可以让患者及家属、高危人群掌握糖尿病的防治知识, 提高自我保健意识, 提高现症患者就诊率, 减少、延缓并发症的发生, 延长寿命, 降低糖尿病病死率, 同时提高糖尿病患者的生活质量, 健康教育作为糖尿病综合防治5项措施之一, 在糖尿病防治中发挥了重要...
2018, 39(04): 135-140.
Abstract:
Lynch syndrome (LS) is the most common hereditary colorectal cancer syndrome, an autosomal dominant inherited disease which is caused by mismatch repair system defect. Learning knowledge in the genetics and diagnosis of LS, and identifying at-risk pa...
Lynch syndrome (LS) is the most common hereditary colorectal cancer syndrome, an autosomal dominant inherited disease which is caused by mismatch repair system defect. Learning knowledge in the genetics and diagnosis of LS, and identifying at-risk pa...
2018, 39(04): 141-144.
Abstract:
Objective To explore the feasibility and function in improving the information literacy of medical students by using the modularization and project-driven teaching in the teaching of Medical Information Retrieval.Me thods A total number of 197 studen...
Objective To explore the feasibility and function in improving the information literacy of medical students by using the modularization and project-driven teaching in the teaching of Medical Information Retrieval.Me thods A total number of 197 studen...
2018, 39(04): 145-148.
Abstract:
Objective To explore the application of Bloom's Taxonomy of Educational Objectives in the Department of Gynecology in the theoretical teaching. Me thods The research subjects were 119 medical students in Kunming Medical University from November 2017 ...
Objective To explore the application of Bloom's Taxonomy of Educational Objectives in the Department of Gynecology in the theoretical teaching. Me thods The research subjects were 119 medical students in Kunming Medical University from November 2017 ...
