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RSS2016 Vol. 37, No. 04
2016, 37(04)
Abstract:
［Abstract］Facial wrinkles are the most common cosmetic problem，expression-rich people are more prone to produce wrinkles at frontal and eye region. The main structural and dynamic basis of crow's feet is the contraction of the lateral part of orbicularis oculi and its close attachment with the skin. With the development of cosmetology technique，lots of applied anatomic researches related to the primary structure，nerve innervations and microstructure of the orbicularis oculi were performed by clinical doctors and anatomic scholars，and diversity of strategies to attenuate crow's feet were designed. Here is a review to these researches.
［Abstract］Facial wrinkles are the most common cosmetic problem，expression-rich people are more prone to produce wrinkles at frontal and eye region. The main structural and dynamic basis of crow's feet is the contraction of the lateral part of orbicularis oculi and its close attachment with the skin. With the development of cosmetology technique，lots of applied anatomic researches related to the primary structure，nerve innervations and microstructure of the orbicularis oculi were performed by clinical doctors and anatomic scholars，and diversity of strategies to attenuate crow's feet were designed. Here is a review to these researches.
2016, 37(04)
Abstract:
［Abstract］Objective To investigate the in vitro anticancer effects of the bioactive fraction AMH-T on human cancer cell lines Hela，U251，HepG2 and the dose-effect and time-effect relationship. Methods MTT assay was used to evaluate the anticancer effect of AMH-T in vitro. The experiment set the negative group（DMSO）， the positive group（DDP，3 μM）， five dosage groups of AMH-T（2， 4， 8， 16， 32 μg/mL）. The tumor inhibition rate in each group was tested by MTT assay on 24 h， 48 h and 72 h after treatment. Results After 72h treatment with 8 μg/ml of AMH-T，most Hela cells died under inverted microscope. The inhibitory ratios of AMH-T at 2， 4， 8， 16， 32 μg/mL on Hela cells were -5.54%， 2.93%， 72.14%， 74.41% and 73.86%; 3.87%， 12.50%， 54.07%， 55.27% and 51.86% on HepG2 cells， and -5.47%， 1.08%， 54.83%， 57.75% and 71.78% on U251 cells. The IC50 values against Hela， HepG2 and U251 were 11.66 μg， 22.90 μg，and 16.85 μg，respectively. Conclusions AMH-T has significant anticancer effects on Hela，HepG2 and U251 cells in a concentration and time dependent manner. Hela cell line is most sensitive to AMH-T among three cell lines.
［Abstract］Objective To investigate the in vitro anticancer effects of the bioactive fraction AMH-T on human cancer cell lines Hela，U251，HepG2 and the dose-effect and time-effect relationship. Methods MTT assay was used to evaluate the anticancer effect of AMH-T in vitro. The experiment set the negative group（DMSO）， the positive group（DDP，3 μM）， five dosage groups of AMH-T（2， 4， 8， 16， 32 μg/mL）. The tumor inhibition rate in each group was tested by MTT assay on 24 h， 48 h and 72 h after treatment. Results After 72h treatment with 8 μg/ml of AMH-T，most Hela cells died under inverted microscope. The inhibitory ratios of AMH-T at 2， 4， 8， 16， 32 μg/mL on Hela cells were -5.54%， 2.93%， 72.14%， 74.41% and 73.86%; 3.87%， 12.50%， 54.07%， 55.27% and 51.86% on HepG2 cells， and -5.47%， 1.08%， 54.83%， 57.75% and 71.78% on U251 cells. The IC50 values against Hela， HepG2 and U251 were 11.66 μg， 22.90 μg，and 16.85 μg，respectively. Conclusions AMH-T has significant anticancer effects on Hela，HepG2 and U251 cells in a concentration and time dependent manner. Hela cell line is most sensitive to AMH-T among three cell lines.
2016, 37(04)
Abstract:
［Abstract］Objective To construct and identify shRNA-Bmi1 recombinant vector in vitro， and to explore the effects of target silencing proto oncogene Bmi1 on the Bmi1mRNA and protein expression in human gallbladder carcinoma cell and the expression of hTERT on subcutaneously transplantated tumor of human gallbladder carcinoma in BALB/c Nude Mice. Methods Four shRNABmi1 recombinant plasmids were successfully constructed according to different Bmi1 sites. Transfection efficiency was observed by inverted fluorescence microscope and RT-PCR and Western blot were used to measure mRNA and protein expression of Bmi-1 in gallbladder cancer cells. The effective interference group was selected for the vivo experiment. The subcutaneous transplantation tumor model of human GBC-SD cell line was successfully constructed in BALB/c nude Mice and randomly divided into shRNA-Bmi-4 group（experimental group），shRNA-Scramble（mismatch group），Lipofectamine（negative control group），GBC-SD（blank control group）. After established the subcutaneous transplantation tumor model， the nude mice were killed after the treatment for 6 weeks. The expression level of hTERT protein in nude mice transplanted tumor was detected by immunohistochemistry. Results shRNA-Bmi1 recombinant vector was successfully constructed. The most effective interferencing plasmids were transfected into GBC-SD cells for 48 hour. The highest green fluorescence intensity and transfection efficiency of shRNA-Bmi1-4 group GBC-SD were observed by inverted fluorescence microscope. RT-PCR and Western blot results showed that the expression levels of Bmi1mRNA and protein in shRNA-Bmi1-4 group were significantly lower than that in the control groups （P < 0.05）， and there was no significant difference between control groups（P > 0.05）.The shRNA-Bmi1-4 group was selected as the effective interference sequence for the following in vivo experiments. The expression level of hTERT protein on transplantation tumor in nude mice in shRNA-Bmi-4 group was significantly lower than that in control group（P < 0.05）， and there was no significant difference between the control groups（P > 0.05）. Conclusions shRNA-Bmi1 recombinant vector has been successfully constructed in vitro， and the transformation and transfection experiments of the recombinant vector have been carried out successfully. Targeted silencing Bmi1 gene can effectively promote Bmi1mRNA degradation，inhibit the Bmil protein expression in the GBC-SD cell line and the expression of hTERT protein in subcutaneously transplantated tumor of GBC-SD in Nude Mice.
［Abstract］Objective To construct and identify shRNA-Bmi1 recombinant vector in vitro， and to explore the effects of target silencing proto oncogene Bmi1 on the Bmi1mRNA and protein expression in human gallbladder carcinoma cell and the expression of hTERT on subcutaneously transplantated tumor of human gallbladder carcinoma in BALB/c Nude Mice. Methods Four shRNABmi1 recombinant plasmids were successfully constructed according to different Bmi1 sites. Transfection efficiency was observed by inverted fluorescence microscope and RT-PCR and Western blot were used to measure mRNA and protein expression of Bmi-1 in gallbladder cancer cells. The effective interference group was selected for the vivo experiment. The subcutaneous transplantation tumor model of human GBC-SD cell line was successfully constructed in BALB/c nude Mice and randomly divided into shRNA-Bmi-4 group（experimental group），shRNA-Scramble（mismatch group），Lipofectamine（negative control group），GBC-SD（blank control group）. After established the subcutaneous transplantation tumor model， the nude mice were killed after the treatment for 6 weeks. The expression level of hTERT protein in nude mice transplanted tumor was detected by immunohistochemistry. Results shRNA-Bmi1 recombinant vector was successfully constructed. The most effective interferencing plasmids were transfected into GBC-SD cells for 48 hour. The highest green fluorescence intensity and transfection efficiency of shRNA-Bmi1-4 group GBC-SD were observed by inverted fluorescence microscope. RT-PCR and Western blot results showed that the expression levels of Bmi1mRNA and protein in shRNA-Bmi1-4 group were significantly lower than that in the control groups （P < 0.05）， and there was no significant difference between control groups（P > 0.05）.The shRNA-Bmi1-4 group was selected as the effective interference sequence for the following in vivo experiments. The expression level of hTERT protein on transplantation tumor in nude mice in shRNA-Bmi-4 group was significantly lower than that in control group（P < 0.05）， and there was no significant difference between the control groups（P > 0.05）. Conclusions shRNA-Bmi1 recombinant vector has been successfully constructed in vitro， and the transformation and transfection experiments of the recombinant vector have been carried out successfully. Targeted silencing Bmi1 gene can effectively promote Bmi1mRNA degradation，inhibit the Bmil protein expression in the GBC-SD cell line and the expression of hTERT protein in subcutaneously transplantated tumor of GBC-SD in Nude Mice.
2016, 37(04)
Abstract:
［Abstract］Objective To explore the influence of PcDNA3.0-WWOX eukaryotic expression vector on the expression of WWOXmRNA，protein and transmembrane potential of mitochondrion（△Ψm） in human gallbladder carcinoma cells. Methods Total RNA in the human gallbladder carcinoma cells was extracted. Then the cDNA of WWOX gene was synthesized by RT-PCR and PCR products were reclaimed and purified. Both of WWOX gene and pcDNA3.0 vector were cut by restriction enzymes ECOR Ⅰ and XhoⅠ. The double enzyme WWOX fragments and pcDNA 3.0 vector were restructured under the action of T4 DNA ligase. After PcDNA 3.0-WWOX recombinant plasmid transformation，the extraction of the plasmid by double enzyme cutting was sent to identification and sequencing identification. Then the pcDNA 3.0-WWOX recombinant plasmid was used to transfect gallbladder cancer GBC-SD cells. RT-PCR and Western Blot were used to detect the expression level of WWOX gene and JC-1 dyeing was used to detect the transmembrane potential （△Ψm） of gallbladder cancer cells' mitochondria．Results We successfully construct pcDNA3.0 - WWOX recombinant plasmid，and the sequencing analysis showed that the DNA sequence was the same to WWOX sequence in Gene Bank. pcDNA3.0 - WWOX recombinant plasmid was successfully transfected to gallbladder cancer GBC- SD cells. RT-PCR and Western blot results showed that 48 hours after plasmid transfection in gallbladder cancer GBC-SD cells，the mRNA gray ratio of pcDNA-3.0-WWOX group was obviously higher than that of the control groups（P＜0.05），the amount of mRNA expression between the control groups had no significant difference（P＞0.05）. The protein gray ratio of pcDNA-3.0-WWOX group was obviously higher than that of the control group（P＜0.05）， and the amount of protein expression between the control groups had no significant difference（P＞0.05）. The green fluorescent signals in mitochondria in cells in the pcDNA-3.0-WWOX group was higher than the control groups（P＜0.05），while had no significant difference between the control groups（P＞0.05）. Conclusions We have successfully constructed PcDNA3.0-WWOX recombinant vector and performed transformation and transfection of this vector in vitro. Targeted PcDNA3.0 - WWOX recombinant vector can effectively inhibit WWOX mRNA degradation in the gallbladder cancer cells， effectively promote the expression of the protein and early apoptosis of the cells. WWOX gene may participate in mitochondria dependent apoptotic pathways which regulates gallbladder cancer cells growth.
［Abstract］Objective To explore the influence of PcDNA3.0-WWOX eukaryotic expression vector on the expression of WWOXmRNA，protein and transmembrane potential of mitochondrion（△Ψm） in human gallbladder carcinoma cells. Methods Total RNA in the human gallbladder carcinoma cells was extracted. Then the cDNA of WWOX gene was synthesized by RT-PCR and PCR products were reclaimed and purified. Both of WWOX gene and pcDNA3.0 vector were cut by restriction enzymes ECOR Ⅰ and XhoⅠ. The double enzyme WWOX fragments and pcDNA 3.0 vector were restructured under the action of T4 DNA ligase. After PcDNA 3.0-WWOX recombinant plasmid transformation，the extraction of the plasmid by double enzyme cutting was sent to identification and sequencing identification. Then the pcDNA 3.0-WWOX recombinant plasmid was used to transfect gallbladder cancer GBC-SD cells. RT-PCR and Western Blot were used to detect the expression level of WWOX gene and JC-1 dyeing was used to detect the transmembrane potential （△Ψm） of gallbladder cancer cells' mitochondria．Results We successfully construct pcDNA3.0 - WWOX recombinant plasmid，and the sequencing analysis showed that the DNA sequence was the same to WWOX sequence in Gene Bank. pcDNA3.0 - WWOX recombinant plasmid was successfully transfected to gallbladder cancer GBC- SD cells. RT-PCR and Western blot results showed that 48 hours after plasmid transfection in gallbladder cancer GBC-SD cells，the mRNA gray ratio of pcDNA-3.0-WWOX group was obviously higher than that of the control groups（P＜0.05），the amount of mRNA expression between the control groups had no significant difference（P＞0.05）. The protein gray ratio of pcDNA-3.0-WWOX group was obviously higher than that of the control group（P＜0.05）， and the amount of protein expression between the control groups had no significant difference（P＞0.05）. The green fluorescent signals in mitochondria in cells in the pcDNA-3.0-WWOX group was higher than the control groups（P＜0.05），while had no significant difference between the control groups（P＞0.05）. Conclusions We have successfully constructed PcDNA3.0-WWOX recombinant vector and performed transformation and transfection of this vector in vitro. Targeted PcDNA3.0 - WWOX recombinant vector can effectively inhibit WWOX mRNA degradation in the gallbladder cancer cells， effectively promote the expression of the protein and early apoptosis of the cells. WWOX gene may participate in mitochondria dependent apoptotic pathways which regulates gallbladder cancer cells growth.
2016, 37(04)
Abstract:
［Abstract］Objective To investigate the effect of Fuzhengguben Jelly on rat hypothalamus-adrenal cortex system．Methods SD rats were administered with hydrocortisone to induce kidney yang deficiency and Fuzhengguben jelly for treatment for continuousl 35 days. The body weight was weighed every one week. On day 17， 10-mg sterile cotton balls were implanted in both forelimb footpaths of the animals. At the end of the experiment， the rats were anesthetized and the the left adrenal glands removed followed by ACTH attack immediately. After another 15 min，the right adrenal glands were removed. Then the blood was collected and plasma CORT was measured. The tampon granulation，thymus，adrenal gland were also removed and weighed. Meanwhile， the difference in Vc content of the both adrenal glands was analyzed. Results（1）From the first week after modeling， the weights of the animals in model group and treatment group were significantly decreased， and dull hair and hunch back were observed，suggesting a obvious symptoms of kidney yang deficiency.（2）After treatment for 35 days， the various doses of Fuzhengguben jelly markedly suppressed rat thymus. There were no significant differences in tampon granuloma dry and wet weight between the model group and the Fuzhengguben jelly group. （3）In addition， Fuzhengguben jelly and positive drug remarkably elevated the Vc content difference between the left and the right adrenal gland，and had significant effect on CORT. Conclusions Fuzhengguben jelly has significant effects on immune and hypothalamus-adrenal cortex systems of the rats.
［Abstract］Objective To investigate the effect of Fuzhengguben Jelly on rat hypothalamus-adrenal cortex system．Methods SD rats were administered with hydrocortisone to induce kidney yang deficiency and Fuzhengguben jelly for treatment for continuousl 35 days. The body weight was weighed every one week. On day 17， 10-mg sterile cotton balls were implanted in both forelimb footpaths of the animals. At the end of the experiment， the rats were anesthetized and the the left adrenal glands removed followed by ACTH attack immediately. After another 15 min，the right adrenal glands were removed. Then the blood was collected and plasma CORT was measured. The tampon granulation，thymus，adrenal gland were also removed and weighed. Meanwhile， the difference in Vc content of the both adrenal glands was analyzed. Results（1）From the first week after modeling， the weights of the animals in model group and treatment group were significantly decreased， and dull hair and hunch back were observed，suggesting a obvious symptoms of kidney yang deficiency.（2）After treatment for 35 days， the various doses of Fuzhengguben jelly markedly suppressed rat thymus. There were no significant differences in tampon granuloma dry and wet weight between the model group and the Fuzhengguben jelly group. （3）In addition， Fuzhengguben jelly and positive drug remarkably elevated the Vc content difference between the left and the right adrenal gland，and had significant effect on CORT. Conclusions Fuzhengguben jelly has significant effects on immune and hypothalamus-adrenal cortex systems of the rats.
2016, 37(04)
Abstract:
［Abstract］Objective To explore the effects of endocannabinoid system on alcohol-induced liver cell injury. Methods Alcohol-induced liver cell injury model was established by incubating human liver cell（L-02）and human hepatoma cell（HepG2）with the medium containing alcohol. We screened URB937 concentration range， and evaluated its effecst on alcohol-induced liver cell injury. Results Treatment with URB937（0.1～50 μmol/L） alone didn’t affect L-02 and HepG2 cell viability（P＞0.05）. Under the situation of alcohol-induced liver cell injury， URB937 co-treatment induced significant cell death and necrosis（P＜0.05）. Conclusions Elevation of endogenous anandamide concentration would aggravate the cell injury induced by alcohol.
［Abstract］Objective To explore the effects of endocannabinoid system on alcohol-induced liver cell injury. Methods Alcohol-induced liver cell injury model was established by incubating human liver cell（L-02）and human hepatoma cell（HepG2）with the medium containing alcohol. We screened URB937 concentration range， and evaluated its effecst on alcohol-induced liver cell injury. Results Treatment with URB937（0.1～50 μmol/L） alone didn’t affect L-02 and HepG2 cell viability（P＞0.05）. Under the situation of alcohol-induced liver cell injury， URB937 co-treatment induced significant cell death and necrosis（P＜0.05）. Conclusions Elevation of endogenous anandamide concentration would aggravate the cell injury induced by alcohol.
2016, 37(04)
Abstract:
［Abstract］Objective To observe the chemotaxis of blood monocyte to three kinds of glioma cell lines（U87，U251 and T98G），and discuss the source of tumor associated macrophages in glioma and its clinicopathologic significance. Method Transwell assay was applied to observe the chemotaxis of blood monocyte to three kinds of glioma cell lines（U87，U251 and T98G）. Results The chemotaxis of blood monocyte to the three kinds of glioma cell lines（U87，U251 and T98G）was higher than that in the control group（P＜0.01）. The chemotaxis of blood monocyte to U87 was higher than that of U251 and T98G（P=0.001）. The chemotaxis of blood monocyte to U251 was higher than that of T98G（P＝0.03）. Conclusion The three kinds of glioma cell lines all have the ability to chemoattract blood monocyte, suggesting that blood monocytes are one of the important sources of glioma associated macrophages，and play an important role in the occurrence or development of the glioma.
［Abstract］Objective To observe the chemotaxis of blood monocyte to three kinds of glioma cell lines（U87，U251 and T98G），and discuss the source of tumor associated macrophages in glioma and its clinicopathologic significance. Method Transwell assay was applied to observe the chemotaxis of blood monocyte to three kinds of glioma cell lines（U87，U251 and T98G）. Results The chemotaxis of blood monocyte to the three kinds of glioma cell lines（U87，U251 and T98G）was higher than that in the control group（P＜0.01）. The chemotaxis of blood monocyte to U87 was higher than that of U251 and T98G（P=0.001）. The chemotaxis of blood monocyte to U251 was higher than that of T98G（P＝0.03）. Conclusion The three kinds of glioma cell lines all have the ability to chemoattract blood monocyte, suggesting that blood monocytes are one of the important sources of glioma associated macrophages，and play an important role in the occurrence or development of the glioma.
2016, 37(04)
Abstract:
［Abstract］Objective To explore the role of prostaglandin E2 （PGE2） in the pathogenesis of chronic obstructive pulmonary disease（COPD）and lung cancer. Methods Sixty rats were randomly divided into A， B and C groups， 20 in each group. Rats in group A were given intratracheal injection of 3，4-benzopyrene-coin oil mixture to induce lung cancer model. 20-week later all rats were given CT scans of the lungs，then the lung lesion were observed and performed by pathological examination. Rats in group B were given passive smoking and injection of lipopolysaccharide to induce the COPD model，lung pathological examination was given on 29day to observe the COPD pathology. Rats in group C received no treatment and as control. PGE2 Elisa Kit was used to test the serum levels of PGE2 of rats in group A，B，and C. The data were analyzed by using statistical software SPSS 17.0. P＜0.05 was regarded as a statistically significant difference. Results The serum levels of PGE2 in lung cancer group and COPD model group were significantly higher than those in control group，P＜0.05， the difference was significant. Conclusion Our research confirms the high serum levels of PGE2 in lung cancer and COPD， suggesting that COPD and lung cancer may have common pathogenesis.
［Abstract］Objective To explore the role of prostaglandin E2 （PGE2） in the pathogenesis of chronic obstructive pulmonary disease（COPD）and lung cancer. Methods Sixty rats were randomly divided into A， B and C groups， 20 in each group. Rats in group A were given intratracheal injection of 3，4-benzopyrene-coin oil mixture to induce lung cancer model. 20-week later all rats were given CT scans of the lungs，then the lung lesion were observed and performed by pathological examination. Rats in group B were given passive smoking and injection of lipopolysaccharide to induce the COPD model，lung pathological examination was given on 29day to observe the COPD pathology. Rats in group C received no treatment and as control. PGE2 Elisa Kit was used to test the serum levels of PGE2 of rats in group A，B，and C. The data were analyzed by using statistical software SPSS 17.0. P＜0.05 was regarded as a statistically significant difference. Results The serum levels of PGE2 in lung cancer group and COPD model group were significantly higher than those in control group，P＜0.05， the difference was significant. Conclusion Our research confirms the high serum levels of PGE2 in lung cancer and COPD， suggesting that COPD and lung cancer may have common pathogenesis.
2016, 37(04)
Abstract:
［Abstract］Objective To study the expression characteristics of SOX4， BRMS-1， CYTB and P53 in breast cancer. Method We selected 35 patients with breast cancer and collected their cancer tissue and adjacent normal tissue，used TRIzol to extract total RNA and real-time quantitative PCR to detect relative gene expression levels. Results The relative expression levels of SOX4 and BRMS-1 in cancer tissue were significantly higher than in adjacent normal tissue（P＜0.05）. The relative expression levels of BRMS-1 in cancer tissue were significantly lower than in adjacent normal tissue（P＜0.05）.There was a significant correlation between the expression of SOX4 and BRMS-1 （r＝0.57，P＜0.05）in cancer tissue and a significant negative correlation between the expression of SOX4 and CYTB in adjacent normal tissue（r＝-0.64，P＜0.05）. Conclusion The relative expression levels of SOX4 in cancer tissue are significantly high and have a significant correlation with BRMS-1，CYTB in breast cancer.
［Abstract］Objective To study the expression characteristics of SOX4， BRMS-1， CYTB and P53 in breast cancer. Method We selected 35 patients with breast cancer and collected their cancer tissue and adjacent normal tissue，used TRIzol to extract total RNA and real-time quantitative PCR to detect relative gene expression levels. Results The relative expression levels of SOX4 and BRMS-1 in cancer tissue were significantly higher than in adjacent normal tissue（P＜0.05）. The relative expression levels of BRMS-1 in cancer tissue were significantly lower than in adjacent normal tissue（P＜0.05）.There was a significant correlation between the expression of SOX4 and BRMS-1 （r＝0.57，P＜0.05）in cancer tissue and a significant negative correlation between the expression of SOX4 and CYTB in adjacent normal tissue（r＝-0.64，P＜0.05）. Conclusion The relative expression levels of SOX4 in cancer tissue are significantly high and have a significant correlation with BRMS-1，CYTB in breast cancer.
2016, 37(04)
Abstract:
［Abstract］Objective To study the expression of GP73 in the serum and tissue of patients with chronic hepatitis B， liver cirrhosis and primary liver cancer. Methods（1）Double-antibody sandwich ELISA was used to test the level of GP 73 in serum and tissue of of patients with chronic hepatitis B， liver cirrhosis and primary liver cancer and normal control group. 2. Immunohistochemical SP methods were used to test the level of GP 73 in serum and tissue of of patients with chronic hepatitis B， liver cirrhosis and primary liver cancer and normal control group. Results The expression of GP73 in the serum and tissue was irrelevant to sex and age. The expression levels of GP73 were increasing gradually in the patients with chronic hepatitis B and liver cirrhosis . GP73 was relevant to the progression of Chronic hepatitis B. The concentration of GP73 in the serum and tissue of the patients with chronic hepatitis B was relevant to AFP，HBV-DNA，and had a significant correlation with ALP．Conclusion The expression of GP73 seems closely related to the occurrence and progression of hepatocarcinoma， which will be useful in the diagnosis and prognostic evaluation of hepatocarcinoma and in the conjecture of hepatic neoplasm and identification of Malignant Diseases，and will be a valuable marker of hepatocarcinoma.
［Abstract］Objective To study the expression of GP73 in the serum and tissue of patients with chronic hepatitis B， liver cirrhosis and primary liver cancer. Methods（1）Double-antibody sandwich ELISA was used to test the level of GP 73 in serum and tissue of of patients with chronic hepatitis B， liver cirrhosis and primary liver cancer and normal control group. 2. Immunohistochemical SP methods were used to test the level of GP 73 in serum and tissue of of patients with chronic hepatitis B， liver cirrhosis and primary liver cancer and normal control group. Results The expression of GP73 in the serum and tissue was irrelevant to sex and age. The expression levels of GP73 were increasing gradually in the patients with chronic hepatitis B and liver cirrhosis . GP73 was relevant to the progression of Chronic hepatitis B. The concentration of GP73 in the serum and tissue of the patients with chronic hepatitis B was relevant to AFP，HBV-DNA，and had a significant correlation with ALP．Conclusion The expression of GP73 seems closely related to the occurrence and progression of hepatocarcinoma， which will be useful in the diagnosis and prognostic evaluation of hepatocarcinoma and in the conjecture of hepatic neoplasm and identification of Malignant Diseases，and will be a valuable marker of hepatocarcinoma.
2016, 37(04)
Abstract:
［Abstract］Objective To investigate the clinical application value of liquid based cytology（LBC） in differential diagnosis of benign and malignant hydrothorax and ascites．Methods Nine hundreds and forty-seven cases of hydrothorax and ascites samples， including 752 cases of hydrothorax and 195 cases of ascites， were collected. Liquid based cytology（LBC） test and conventional cytology（CC）test were performed to differentially diagnose benign and malignant hydrothorax and ascites， and the difference in the quality and satisfaction of cell slide preparation and the diagnostic results were compared between the two cytology tests．Results（1） Cell slide prepared with CC: the positive cells were few and scattered and had more overlap， the cell distribution was thick and uneven， the background was not clear and often interfered by red blood cells and inflammatory exudate. Cell slide prepared with LBC: the positive cells were more and concentrated and had no overlap， the cell distribution was uniform and in monolayer， the background was clean and clear. The satisfaction rate of slide preparation with CC and LBC was 86.5% and 95.2%， respectively（P＜0.05）. （2）In the total 947 cases of hydrothorax and ascites，the positive rate（27.2%）of LBC test was a little higher than CC test（27.2% vs. 24.6%），but the difference had no statistical significance（P＞0.05）. Conclusion Liquid based cytology is better than conventional cytology in the quality and satisfaction of the cell slide preparation of hydrothorax and ascites samples， and has a little higher positive diagnostic rate of malignant hydrothorax and ascites.
［Abstract］Objective To investigate the clinical application value of liquid based cytology（LBC） in differential diagnosis of benign and malignant hydrothorax and ascites．Methods Nine hundreds and forty-seven cases of hydrothorax and ascites samples， including 752 cases of hydrothorax and 195 cases of ascites， were collected. Liquid based cytology（LBC） test and conventional cytology（CC）test were performed to differentially diagnose benign and malignant hydrothorax and ascites， and the difference in the quality and satisfaction of cell slide preparation and the diagnostic results were compared between the two cytology tests．Results（1） Cell slide prepared with CC: the positive cells were few and scattered and had more overlap， the cell distribution was thick and uneven， the background was not clear and often interfered by red blood cells and inflammatory exudate. Cell slide prepared with LBC: the positive cells were more and concentrated and had no overlap， the cell distribution was uniform and in monolayer， the background was clean and clear. The satisfaction rate of slide preparation with CC and LBC was 86.5% and 95.2%， respectively（P＜0.05）. （2）In the total 947 cases of hydrothorax and ascites，the positive rate（27.2%）of LBC test was a little higher than CC test（27.2% vs. 24.6%），but the difference had no statistical significance（P＞0.05）. Conclusion Liquid based cytology is better than conventional cytology in the quality and satisfaction of the cell slide preparation of hydrothorax and ascites samples， and has a little higher positive diagnostic rate of malignant hydrothorax and ascites.
2016, 37(04)
Abstract:
［Abstract］Objective To discuss the relationship between the expression of E-cadherin and Vimentin and pathological characteristics in intrahepatic cholangiocarcinoma（ICC）. Methods The expression of E-cadherin and Vimentin was detected by immunohistochemistry in ICC and para-carcinoma intrahepatic bile duct（n＝50）.The correlation between the expression of tumor tissues and the clinicopathological characteristics of ICC was analyzed， and their prognostic implication was evaluated. Results The expression of E-cadherin was significantly lower in ICC than in matched as the upper mentioned tissues （42%vs68%，P＜0.05）.The expression of Vimentin was significantly higher in intrahepatic cholangiocarcinoma （ICC） than matched as the upper mentioned tissues（50%vs30%，P＜0.05）. Down-regulation of E-cadherin and up-regulation of Vimentin were significantly associated with TNM stage，differentiation，lymph node metastasis of ICC （P< 0.05）.For the survival analyses，low E-cadherin expression and positive Vimentin expression were significantly associated with shorter survival（P＜0.05）．Conclusion Low expression of E-cadherin and high expression of Vimentin in the intrahepatic cholangiocarcinoma（ICC）are correlated with the invasion， migration and poor prognosis， and have clinical significance in judging the prognosis of patients.
［Abstract］Objective To discuss the relationship between the expression of E-cadherin and Vimentin and pathological characteristics in intrahepatic cholangiocarcinoma（ICC）. Methods The expression of E-cadherin and Vimentin was detected by immunohistochemistry in ICC and para-carcinoma intrahepatic bile duct（n＝50）.The correlation between the expression of tumor tissues and the clinicopathological characteristics of ICC was analyzed， and their prognostic implication was evaluated. Results The expression of E-cadherin was significantly lower in ICC than in matched as the upper mentioned tissues （42%vs68%，P＜0.05）.The expression of Vimentin was significantly higher in intrahepatic cholangiocarcinoma （ICC） than matched as the upper mentioned tissues（50%vs30%，P＜0.05）. Down-regulation of E-cadherin and up-regulation of Vimentin were significantly associated with TNM stage，differentiation，lymph node metastasis of ICC （P< 0.05）.For the survival analyses，low E-cadherin expression and positive Vimentin expression were significantly associated with shorter survival（P＜0.05）．Conclusion Low expression of E-cadherin and high expression of Vimentin in the intrahepatic cholangiocarcinoma（ICC）are correlated with the invasion， migration and poor prognosis， and have clinical significance in judging the prognosis of patients.
2016, 37(04)
Abstract:
［Abstract］Objective To research the long-term effects of palliative surgery and pancreaticoduodenectomy in treatment of pancreatic cancer and ampulla surrounding cancer. Methods Forty-four patients with pancreatic cancer joint periampullary cancer，who were admitted in our hospital for treatment from April 2002 to April 2014， were randomly divided into observation group and control group ， with 22 cases in each group. Patients in the control group were treated with palliative surgery，patients in the observation group were treated with pancreaticoduodenectomy treatment. The blood loss，operative time，hospital stay，complications， survival of change，radical resection rate and relapse rate changes of two groups were compared．Results Compared with control group，in the observation group，the blood loss increased， and the time of the surgery extended. And the difference was statistically significant（P <0.05）. The hospital stay of patients in the two groups were compared， and there was no significant difference（P＞0.05）. The overall incidence of complications of the observation group （54.55%）was significantly higher than that of the control group（22.73%），and the difference was statistically significant（P＜0.05）. The complication rate of the observation group was significantly higher than that of the control group，and the difference was statistically significant（P＜0.05）. Survival status of the two groups were compared， in the observation group， the number of survival cases during 2 years increased more significantly than that of the control group， and the difference was statistically significant（P＜0.05）. The radical resection rate after surgery and recurrence rate of the two group were compared， and the difference was not statistically significant （P> 0.05）. Conclusions Compared with palliative surgery in treatment of pancreatic cancer joint periampullary cancer， pancreatoduodenectomy treatment may increase the blood loss，extend the operative time and increase the complication rate， but significantly improve the long-term survival rate.
［Abstract］Objective To research the long-term effects of palliative surgery and pancreaticoduodenectomy in treatment of pancreatic cancer and ampulla surrounding cancer. Methods Forty-four patients with pancreatic cancer joint periampullary cancer，who were admitted in our hospital for treatment from April 2002 to April 2014， were randomly divided into observation group and control group ， with 22 cases in each group. Patients in the control group were treated with palliative surgery，patients in the observation group were treated with pancreaticoduodenectomy treatment. The blood loss，operative time，hospital stay，complications， survival of change，radical resection rate and relapse rate changes of two groups were compared．Results Compared with control group，in the observation group，the blood loss increased， and the time of the surgery extended. And the difference was statistically significant（P <0.05）. The hospital stay of patients in the two groups were compared， and there was no significant difference（P＞0.05）. The overall incidence of complications of the observation group （54.55%）was significantly higher than that of the control group（22.73%），and the difference was statistically significant（P＜0.05）. The complication rate of the observation group was significantly higher than that of the control group，and the difference was statistically significant（P＜0.05）. Survival status of the two groups were compared， in the observation group， the number of survival cases during 2 years increased more significantly than that of the control group， and the difference was statistically significant（P＜0.05）. The radical resection rate after surgery and recurrence rate of the two group were compared， and the difference was not statistically significant （P> 0.05）. Conclusions Compared with palliative surgery in treatment of pancreatic cancer joint periampullary cancer， pancreatoduodenectomy treatment may increase the blood loss，extend the operative time and increase the complication rate， but significantly improve the long-term survival rate.
2016, 37(04)
Abstract:
［Abstract］Objective To discuss the surgical strategy and clinical effect of PELD（Percutaneous endoscopic lumbar discectomy）in treatment of Lumbar intervertebral disc herniation. Methods From August to October in 2013，200 patients with L4-L5 Lumbar intervertebral disc herniation were selected to be treated by PELD. There were 124 male patients and 76 female patients. All of the patients with the average age of 41 were suffering from obvious pain in waist and lower extremities. Under local anesthesia and monitoring of radiography，and with an admission passage from shoulder and axillary region， nucleus pulposus removal was finished by the means of percutaneous endoscope. VSA（Visual Analogue Scale）and ODI（Oswestry Disability Index）were used to evaluate the clinical effect. Results The follow-up visit to 196 patients lasted for 9-24 months in which the average time was 13.5 months. The VSA of preoperative pain in waist and lower extremities was（7.5±1.8）. After operation， the VSA of the pain after 3 months was（3.0±1.5）， the VSA of the pain after 6 months was（2.8±1.3），and the VSA of the pain after 12 months was（2.0±1.5）. There were obvious differences between before operation and after operation （P＜0.01）；before operation，ODI was（58.5±17.8）%， while after operation， ODI was（29.3±10.5）% after 3 months，（20±7.5）% after 6 months，（15.6±6.4）% after 12 months. There were obvious differences between before operation and after operation（P＜0.01）. Conclusions Percutaneous endoscopic treatment of 4/5 lumbar intervertebral disc herniation from shoulder and axillary region is very complete and effective. Selecting patients correctly before operation and adequate radiology evaluation are the key to guaranteeing surgical success.
［Abstract］Objective To discuss the surgical strategy and clinical effect of PELD（Percutaneous endoscopic lumbar discectomy）in treatment of Lumbar intervertebral disc herniation. Methods From August to October in 2013，200 patients with L4-L5 Lumbar intervertebral disc herniation were selected to be treated by PELD. There were 124 male patients and 76 female patients. All of the patients with the average age of 41 were suffering from obvious pain in waist and lower extremities. Under local anesthesia and monitoring of radiography，and with an admission passage from shoulder and axillary region， nucleus pulposus removal was finished by the means of percutaneous endoscope. VSA（Visual Analogue Scale）and ODI（Oswestry Disability Index）were used to evaluate the clinical effect. Results The follow-up visit to 196 patients lasted for 9-24 months in which the average time was 13.5 months. The VSA of preoperative pain in waist and lower extremities was（7.5±1.8）. After operation， the VSA of the pain after 3 months was（3.0±1.5）， the VSA of the pain after 6 months was（2.8±1.3），and the VSA of the pain after 12 months was（2.0±1.5）. There were obvious differences between before operation and after operation （P＜0.01）；before operation，ODI was（58.5±17.8）%， while after operation， ODI was（29.3±10.5）% after 3 months，（20±7.5）% after 6 months，（15.6±6.4）% after 12 months. There were obvious differences between before operation and after operation（P＜0.01）. Conclusions Percutaneous endoscopic treatment of 4/5 lumbar intervertebral disc herniation from shoulder and axillary region is very complete and effective. Selecting patients correctly before operation and adequate radiology evaluation are the key to guaranteeing surgical success.
2016, 37(04)
Abstract:
［Abstract］Objective To investigate the effect of high homocysteine on blood glucose control in type 2 diabetic patients. Method We selected 186 cases of organic disease that without diabetes acute and chronic complications，and ruled out the type 2 diabetic patients with organic disease，these enrolled cases were divided into two groups according to Homocysteine（Hcy）levels，group 1（Simple diabetes）70 cases and group 2 （diabetes with high Hcy）116 cases. According to the different intensity of the drugs，different weights were given to calculated index of hypoglycemic agents，and then the difference of the drug use index between the two groups at the same Condition of HbA1c and FPG was observed．Results In group 1 and group 2，Hcy levels were 8.33 ± 1.16 and 14.53 ± 4.74， respectively；comprehensive treatment indexes were （4.9 ± 2.63）and（7.16 ± 2.16）， respectively. Composite index had a significant difference between the two groups（P < 0.05）; The level of Hcy was positively correlated with the index of Comprehensive drug （r＝0.252， P < 0.01）. Conclusion Glucose lowering therapy for diabetic patients with high Hcy is more complex and difficult than for the simple diabetic patients
［Abstract］Objective To investigate the effect of high homocysteine on blood glucose control in type 2 diabetic patients. Method We selected 186 cases of organic disease that without diabetes acute and chronic complications，and ruled out the type 2 diabetic patients with organic disease，these enrolled cases were divided into two groups according to Homocysteine（Hcy）levels，group 1（Simple diabetes）70 cases and group 2 （diabetes with high Hcy）116 cases. According to the different intensity of the drugs，different weights were given to calculated index of hypoglycemic agents，and then the difference of the drug use index between the two groups at the same Condition of HbA1c and FPG was observed．Results In group 1 and group 2，Hcy levels were 8.33 ± 1.16 and 14.53 ± 4.74， respectively；comprehensive treatment indexes were （4.9 ± 2.63）and（7.16 ± 2.16）， respectively. Composite index had a significant difference between the two groups（P < 0.05）; The level of Hcy was positively correlated with the index of Comprehensive drug （r＝0.252， P < 0.01）. Conclusion Glucose lowering therapy for diabetic patients with high Hcy is more complex and difficult than for the simple diabetic patients
2016, 37(04)
Abstract:
［Abstract］Objective To investigate the specific differentially expressed proteins in sjogren's syndrome by SELDI . Methods We selected the CM10 Chip and SELDI technology to compare the difference in the proteins expression between SS and health， joint swelling pain group and without joint swelling pain group. Results（1）There were 68 different mass spectrum peak between SS group and normal group（P < 0.05）， and identified 11 differently expressed proteins，including 10 of upreguated and 1 downregulated protein. （2）No specific proteins were found in the joints swelling pain group. （3）The 11 different proteins were analyzed respectively with clinical data， but no correlation was found. Conclusions（1）There are difference in serum proteins express between SS patients and normal subjects.（2）The joint swelling pain symptoms in SS patients is non-specific. （3）The alpha 1 chymotrypsin，fibrinogen aa chain，beta amyloid precursor protein and thyroid hormone，β2 -microglobulin are associated with SS and can result in amyloidosis， the mechanism may be associated with amyloidosis.
［Abstract］Objective To investigate the specific differentially expressed proteins in sjogren's syndrome by SELDI . Methods We selected the CM10 Chip and SELDI technology to compare the difference in the proteins expression between SS and health， joint swelling pain group and without joint swelling pain group. Results（1）There were 68 different mass spectrum peak between SS group and normal group（P < 0.05）， and identified 11 differently expressed proteins，including 10 of upreguated and 1 downregulated protein. （2）No specific proteins were found in the joints swelling pain group. （3）The 11 different proteins were analyzed respectively with clinical data， but no correlation was found. Conclusions（1）There are difference in serum proteins express between SS patients and normal subjects.（2）The joint swelling pain symptoms in SS patients is non-specific. （3）The alpha 1 chymotrypsin，fibrinogen aa chain，beta amyloid precursor protein and thyroid hormone，β2 -microglobulin are associated with SS and can result in amyloidosis， the mechanism may be associated with amyloidosis.
2016, 37(04)
Abstract:
［Abstract］Objetive To investgate the correlation of Vitamin D receptor gene FokI single nucleotide polymorphism with diabetic kidney disease in type 2 diabetic patients．Methods The single nucleotide polymorphism of VDR gene in 235 type 2 diabetes， [including 82 normal albuminuria（DKD0 group），66 microalbuminuria（DKDl group ）and 87 macroalbuminuria（DKD2 group）]and 86 normal controls were detected by TaqMan real-time polymerase chain reaction. The genotype frequency and allele frequency and relative clinical data were compared among groups. Results（1）FokI CC，TC，TT genotype frequencies were 25.23%， 53.27%，21.50%，allele C and allele T frequencies were 51.87% and 48.13% in overall populations. （2）The genotype frequency and allele frequency among groups had no statistically significant difference（P＜0.05）. （3） Logistic multiple regression analysis showed that FokI C allele was not related to T2DKD occurrence（OR=2.040，P＝0.05），but was related to T2DKD development（OR＝4.741，P＝0.004）. Conclusion VDR gene FokI C allele is associated with the risk of T2DKD development.
［Abstract］Objetive To investgate the correlation of Vitamin D receptor gene FokI single nucleotide polymorphism with diabetic kidney disease in type 2 diabetic patients．Methods The single nucleotide polymorphism of VDR gene in 235 type 2 diabetes， [including 82 normal albuminuria（DKD0 group），66 microalbuminuria（DKDl group ）and 87 macroalbuminuria（DKD2 group）]and 86 normal controls were detected by TaqMan real-time polymerase chain reaction. The genotype frequency and allele frequency and relative clinical data were compared among groups. Results（1）FokI CC，TC，TT genotype frequencies were 25.23%， 53.27%，21.50%，allele C and allele T frequencies were 51.87% and 48.13% in overall populations. （2）The genotype frequency and allele frequency among groups had no statistically significant difference（P＜0.05）. （3） Logistic multiple regression analysis showed that FokI C allele was not related to T2DKD occurrence（OR=2.040，P＝0.05），but was related to T2DKD development（OR＝4.741，P＝0.004）. Conclusion VDR gene FokI C allele is associated with the risk of T2DKD development.
2016, 37(04)
Abstract:
［Abstract］Objective To study effect of non-biologic artificial liver support systems with Chinese herbs colon dialysis on the serum levels of tumor necrosis factor-alpha，interleukin-6 and endotoxin in the treatment of liver failure. Methods Two hundred patients with hepatic failure，including the treatment group 100 cases and the control group 100 cases. Patients in the two groups received non-biologic artificial liver support systems and internal medicine therapy，patients in the treatment group were also given Chinese herbs colon dialysis with the different Chinese herbal formulas once every day on this basis. The serum TNF-α and IL-6 levels were determined by flow cytometer and serum endotoxin levels were determined by turbidimetric-kinetic method before treatment，1 weeks， 2 weeks，4 weeks after treatment. The differences in serum levels of TNF-α，IL-6 and endotoxin were compared between two groups. Results Compared with control group，the levels of serum TNF-α， IL-6 and endotoxin significantly decreased in patients 4 weeks after treatment in the treatment group（P＜0.05）. The total effective rate of the treatment group was 85%，the total effective rate of the control group was 60%.The therapeutic effect of the treatment group was significantly better than that of the control group （P＜0.05）. A significant positive correlation was found between serum TNF-α levels and serum endotoxin level（the treatment group: r＝0.977，P＝0.023；the control group： r＝0.962，P＝0.038）. A significant positive correlation was found between serum IL-6 levels and serum endotoxin level in the control group（ r＝0.967，P＝0.033）. Conclusion Non-biologic artificial liver support systems with Chinese herbs colon dialysis can significantly decrease the serum levels of TNF-α，IL-6 and endotoxin of patients with hepatic failure，and improve the clinical efficacy.
［Abstract］Objective To study effect of non-biologic artificial liver support systems with Chinese herbs colon dialysis on the serum levels of tumor necrosis factor-alpha，interleukin-6 and endotoxin in the treatment of liver failure. Methods Two hundred patients with hepatic failure，including the treatment group 100 cases and the control group 100 cases. Patients in the two groups received non-biologic artificial liver support systems and internal medicine therapy，patients in the treatment group were also given Chinese herbs colon dialysis with the different Chinese herbal formulas once every day on this basis. The serum TNF-α and IL-6 levels were determined by flow cytometer and serum endotoxin levels were determined by turbidimetric-kinetic method before treatment，1 weeks， 2 weeks，4 weeks after treatment. The differences in serum levels of TNF-α，IL-6 and endotoxin were compared between two groups. Results Compared with control group，the levels of serum TNF-α， IL-6 and endotoxin significantly decreased in patients 4 weeks after treatment in the treatment group（P＜0.05）. The total effective rate of the treatment group was 85%，the total effective rate of the control group was 60%.The therapeutic effect of the treatment group was significantly better than that of the control group （P＜0.05）. A significant positive correlation was found between serum TNF-α levels and serum endotoxin level（the treatment group: r＝0.977，P＝0.023；the control group： r＝0.962，P＝0.038）. A significant positive correlation was found between serum IL-6 levels and serum endotoxin level in the control group（ r＝0.967，P＝0.033）. Conclusion Non-biologic artificial liver support systems with Chinese herbs colon dialysis can significantly decrease the serum levels of TNF-α，IL-6 and endotoxin of patients with hepatic failure，and improve the clinical efficacy.
2016, 37(04)
Abstract:
［Abstract］Objective To evaluate the expression of WT1 gene in leukemia patients and its clinical implication．Method Nest RT-PCR technique was applied to examine WT1 gene expression in 200 patients with leukemia．Results The WT1 mRNA positive rates in acute myeloid leukemia and lymphoblastic leukemia were 79.2%（76/96）and 57.1%（32/56）. In chronic myeloid leukemia，WT1 mRNA was undetectable in chronic phase; but was revealed in 8 of 14 patients at blast crisis（57.1%）. The rate of remission in the patients positive for WT1 was lower than in those negative for WT1（55.7% vs 85.4%，P＜0.005）．Conclusion The WT1 is highly expressed in the majority of the patients with leukemia and thus is involved in human leukemogenesis. WT1 may be a new prognostic factor and a panleukemia marker for the detection of MRD.
［Abstract］Objective To evaluate the expression of WT1 gene in leukemia patients and its clinical implication．Method Nest RT-PCR technique was applied to examine WT1 gene expression in 200 patients with leukemia．Results The WT1 mRNA positive rates in acute myeloid leukemia and lymphoblastic leukemia were 79.2%（76/96）and 57.1%（32/56）. In chronic myeloid leukemia，WT1 mRNA was undetectable in chronic phase; but was revealed in 8 of 14 patients at blast crisis（57.1%）. The rate of remission in the patients positive for WT1 was lower than in those negative for WT1（55.7% vs 85.4%，P＜0.005）．Conclusion The WT1 is highly expressed in the majority of the patients with leukemia and thus is involved in human leukemogenesis. WT1 may be a new prognostic factor and a panleukemia marker for the detection of MRD.
2016, 37(04)
Abstract:
［Abstract］Objective To analyze the clinical effect of between CDSP and CDSP combined with cardiovascular drugs treatment for coronary heart disease. Methods We selected 100 patients with coronary heart disease，and used the random grouping method to divide them into experimental group（n＝50） and control group（n＝50）. Experimental group was given CDSP combined with cardiovascular routine treatment for 1month， the control group was given cardiovascular routine therapy for 1 month. We compared the clinical effect and the serum level of TC，HDL-C，LDL-C，CRP and D-dimer between experimental group and control group．Results After treatment， the clinical effects on angina（P＜0.05） and electrocardiogram（ P＜0.05）of experimental group were better than control group. Before treatment， there was no significant difference between experimental group and control group in the serum levels of TC，HDL-C，LDL-C，CRP and D-dimer （P＞0.05）. After treatment， the serum levels of TC，HDL-C，LDL-C，CRP and D-dimer of experimental group is significant better than control group（P＞0.05）. Conclusions CDSP can effectively relieve the main symptoms of coronary heart disease.
［Abstract］Objective To analyze the clinical effect of between CDSP and CDSP combined with cardiovascular drugs treatment for coronary heart disease. Methods We selected 100 patients with coronary heart disease，and used the random grouping method to divide them into experimental group（n＝50） and control group（n＝50）. Experimental group was given CDSP combined with cardiovascular routine treatment for 1month， the control group was given cardiovascular routine therapy for 1 month. We compared the clinical effect and the serum level of TC，HDL-C，LDL-C，CRP and D-dimer between experimental group and control group．Results After treatment， the clinical effects on angina（P＜0.05） and electrocardiogram（ P＜0.05）of experimental group were better than control group. Before treatment， there was no significant difference between experimental group and control group in the serum levels of TC，HDL-C，LDL-C，CRP and D-dimer （P＞0.05）. After treatment， the serum levels of TC，HDL-C，LDL-C，CRP and D-dimer of experimental group is significant better than control group（P＞0.05）. Conclusions CDSP can effectively relieve the main symptoms of coronary heart disease.
2016, 37(04)
Abstract:
［Abstract］Objective To understand the effect of regulatory T cells on senile osteoporosis by measuring the percentage of regulatory T cells in CD4+T cells in different age groups of male SD rats. Methods Sprague-Dawley male rats which was 6-8 weeks，6 months，12 months，24 months old were selected as subjects. There were 10 rats in each group. We detected bone mineral density of femur，spine，pelvis，whole-body of rats by dual-energy X -ray absorptiometry. We collected blood samples from the heart，used CD25/forkhead box P3 double-positive to describe regulatory T cells and then measured the percentage of regulatory T cells in CD4+T cells by flow cytometry. Results（1）The percentage of regulatory T cells in CD4+T cells in 6-8 weeks，6 months，12 months，24 months old male rats groups were not the same， the difference was statistically significant（P＝0.000）. The percentage of regulatory T cells in CD4+T cells of 6-8weeks old groups was lower than 6 months，12 months，24months old groups， the difference was statistically significant（P＝0.000）. The percentage of regulatory T cells in CD4+T cells of 6 months old groups was lower than 12 months，24 months old groups， the difference was statistically significant（P＜0.05）. The percentage of regulatory T cells in CD4+T cells of 24 months old groups was lower than 12months， but there was no significant difference（P＞0.05）.（2）There was a positively correlation between the percentage of regulatory T cells in CD4+T cells and bone mineral density of femur，spine，pelvis，whole-body. Conclusions （1）The percentage of regulatory T cells in CD4+T cells in four groups are not the same，the lowest at 6-8 weeks of age，by 6 months，there is an increase and when 12 months increase to a maximum then decrease at 24 months of age，indicating that the percentage of regulatory T cells in CD4+T cells in different age groups are significantly different. （2）There is a positive correlation between the percentage of regulatory T cells in CD4+T cells and bone mineral density of femur，spine，pelvis，whole-body，indicating that because of the bone resorption reducing with the percentage of regulatory T cells increasing，the values of bone mineral density raising，when the percentage of regulatory T cells decreasing，the values of bone mineral density declining. In view of the number of regulatory T cells declining with aging，suggesting that exogenous injection of regulatory T cells may inhibit the bone resorption by osteoclast，which may become the new direction of senile osteoporosis treatment.
［Abstract］Objective To understand the effect of regulatory T cells on senile osteoporosis by measuring the percentage of regulatory T cells in CD4+T cells in different age groups of male SD rats. Methods Sprague-Dawley male rats which was 6-8 weeks，6 months，12 months，24 months old were selected as subjects. There were 10 rats in each group. We detected bone mineral density of femur，spine，pelvis，whole-body of rats by dual-energy X -ray absorptiometry. We collected blood samples from the heart，used CD25/forkhead box P3 double-positive to describe regulatory T cells and then measured the percentage of regulatory T cells in CD4+T cells by flow cytometry. Results（1）The percentage of regulatory T cells in CD4+T cells in 6-8 weeks，6 months，12 months，24 months old male rats groups were not the same， the difference was statistically significant（P＝0.000）. The percentage of regulatory T cells in CD4+T cells of 6-8weeks old groups was lower than 6 months，12 months，24months old groups， the difference was statistically significant（P＝0.000）. The percentage of regulatory T cells in CD4+T cells of 6 months old groups was lower than 12 months，24 months old groups， the difference was statistically significant（P＜0.05）. The percentage of regulatory T cells in CD4+T cells of 24 months old groups was lower than 12months， but there was no significant difference（P＞0.05）.（2）There was a positively correlation between the percentage of regulatory T cells in CD4+T cells and bone mineral density of femur，spine，pelvis，whole-body. Conclusions （1）The percentage of regulatory T cells in CD4+T cells in four groups are not the same，the lowest at 6-8 weeks of age，by 6 months，there is an increase and when 12 months increase to a maximum then decrease at 24 months of age，indicating that the percentage of regulatory T cells in CD4+T cells in different age groups are significantly different. （2）There is a positive correlation between the percentage of regulatory T cells in CD4+T cells and bone mineral density of femur，spine，pelvis，whole-body，indicating that because of the bone resorption reducing with the percentage of regulatory T cells increasing，the values of bone mineral density raising，when the percentage of regulatory T cells decreasing，the values of bone mineral density declining. In view of the number of regulatory T cells declining with aging，suggesting that exogenous injection of regulatory T cells may inhibit the bone resorption by osteoclast，which may become the new direction of senile osteoporosis treatment.
2016, 37(04)
Abstract:
［Abstract］Objective To evaluate the effect of osteoporosis on the pathogenesis of osteoarthritis， and analyze the correlation of osteoporosis and osteoarthritis. Methods We statistically analyzed the data of 563 patients with osteoarthritis and 439 patients with osteoarthritis， including the bone mineral density，the method of statistics included relevance analysis，x2 of R×C form analysis and variance analysis．Results There was a statistically significant difference in the rate of osteoporosis between the case group and control group， the rate of osteoporosis in case group was higher than the control group. Conclusion The risk of osteoarthritis for osteoporosis patients is 25.320 times of the patients without osteoporosis， osteoporosis may be involved in the pathogenesis of osteoarthritis.
［Abstract］Objective To evaluate the effect of osteoporosis on the pathogenesis of osteoarthritis， and analyze the correlation of osteoporosis and osteoarthritis. Methods We statistically analyzed the data of 563 patients with osteoarthritis and 439 patients with osteoarthritis， including the bone mineral density，the method of statistics included relevance analysis，x2 of R×C form analysis and variance analysis．Results There was a statistically significant difference in the rate of osteoporosis between the case group and control group， the rate of osteoporosis in case group was higher than the control group. Conclusion The risk of osteoarthritis for osteoporosis patients is 25.320 times of the patients without osteoporosis， osteoporosis may be involved in the pathogenesis of osteoarthritis.
2016, 37(04)
Abstract:
［Abstract］Objective To analyze the risk factors for cardiovascular event in patients with continuous ambulatory peritoneal dialysis （CAPD）in Yunnan. Methods 270 cases of CAPD patients were collected during the period from October 2012 to September 2013 in designated hospital in Yunnan. Among all the cases，no cardiovascular or cerebrovascular events occurred in 178 patients， while another 92 patients developed cardiovascular or cerebrovascular events during 12 months follow-up，multiple demographics，clinical indexes and biochemical indicators of patients in both groups were analyzed. The logistic regression analysis was used to analyze the risk factors for adverse cardiovascular events. Results There was no significant difference in gender，age，educational background， primary disease，dialysis age， blood urea nitrogen and serum creatinine between event group and non-event group（all P＞0.05）. The levels of serum phosphorus，serum intact parathyroid hormone（iPTH），serum total cholesterol，low-density lipoprotein cholesterol（LDL-C），high-sensitivity C-reactive protein （hs-CRP） of event group were higher than those of non-event group，while blood pressure control rates and hemoglobin level of event group were lower than those of non-event group．Conclusion Blood pressure control rates and the levels of serum total cholesterol，LDL-C are important risk factors for adverse cardiovascular events in CAPD patients.
［Abstract］Objective To analyze the risk factors for cardiovascular event in patients with continuous ambulatory peritoneal dialysis （CAPD）in Yunnan. Methods 270 cases of CAPD patients were collected during the period from October 2012 to September 2013 in designated hospital in Yunnan. Among all the cases，no cardiovascular or cerebrovascular events occurred in 178 patients， while another 92 patients developed cardiovascular or cerebrovascular events during 12 months follow-up，multiple demographics，clinical indexes and biochemical indicators of patients in both groups were analyzed. The logistic regression analysis was used to analyze the risk factors for adverse cardiovascular events. Results There was no significant difference in gender，age，educational background， primary disease，dialysis age， blood urea nitrogen and serum creatinine between event group and non-event group（all P＞0.05）. The levels of serum phosphorus，serum intact parathyroid hormone（iPTH），serum total cholesterol，low-density lipoprotein cholesterol（LDL-C），high-sensitivity C-reactive protein （hs-CRP） of event group were higher than those of non-event group，while blood pressure control rates and hemoglobin level of event group were lower than those of non-event group．Conclusion Blood pressure control rates and the levels of serum total cholesterol，LDL-C are important risk factors for adverse cardiovascular events in CAPD patients.
2016, 37(04)
Abstract:
［Abstract］Objective To study the characteristics of stone constituents in Yi nationality patients in Yunnan province，and provide assistance in prevention and treatment of urinary stones in this area. Methods Infrared spectrophotometry was carried out for biochemical composition analysis of 205 samples of urinary stones，and the chemical composition of urinary stones were analyzed with the clinical data．Results The incidence of upper urinary tract stones was 90.7％， and the incidence of lower urinary tract stones was 9.3％. Pure stones were found in 51.2% of all stones and the mixed stones were 48.8 %. Calcium oxalate stones（43.4%）and calcium oxalate + carbonate apatite stones（31.2%） were repectively the most common stones in pure and mixed stones. The component of urinary stones was different in terms of gender. The detection rate of calcium oxalate was higher in male than female. Inversely，the detection rate of carbonate apatite and magnesium ammonium phosphate was lower in male than female．Conclusions In urinary stone patients of Yi nationality in Yunnan province， the incidence of upper urinary tract stones is higher than lower urinary tract stones. Calcium oxalate stones are the most common stones， and the gender has a effect on the component of urinary stones.
［Abstract］Objective To study the characteristics of stone constituents in Yi nationality patients in Yunnan province，and provide assistance in prevention and treatment of urinary stones in this area. Methods Infrared spectrophotometry was carried out for biochemical composition analysis of 205 samples of urinary stones，and the chemical composition of urinary stones were analyzed with the clinical data．Results The incidence of upper urinary tract stones was 90.7％， and the incidence of lower urinary tract stones was 9.3％. Pure stones were found in 51.2% of all stones and the mixed stones were 48.8 %. Calcium oxalate stones（43.4%）and calcium oxalate + carbonate apatite stones（31.2%） were repectively the most common stones in pure and mixed stones. The component of urinary stones was different in terms of gender. The detection rate of calcium oxalate was higher in male than female. Inversely，the detection rate of carbonate apatite and magnesium ammonium phosphate was lower in male than female．Conclusions In urinary stone patients of Yi nationality in Yunnan province， the incidence of upper urinary tract stones is higher than lower urinary tract stones. Calcium oxalate stones are the most common stones， and the gender has a effect on the component of urinary stones.
2016, 37(04)
Abstract:
［Abstract］Objective To investigate the clinical effect of different doses of esomeprazole in treatment of nonvariceal upper gastrointestinal bleeding（ANVUGIB）．Methods We collected the data of 90 patients with ANVUGIB admitted in our hospital from August 2014 to October 2015. The 90 patients were divided into 2 groups: treatment group and control group. Patients in both group were given the combination of Octreotide and other treatment，patients in the treatment group were given continuous pumping of 8 milligrams of esomeprazole per minute for 72 hours after intravenous injection of 80 mg of esomeprazole， patients in the control group were given intravenous instillation of 40 mg of esomeprazole every 12 hours. Then we compared the clinical effect between two groups. Results In the two groups，we statistically analyzed the number of effective cases according to the gender（male and femal），age over 60 years old， and the hemoglobin less than 70.0 grams，there was a statistical significant difference （P < 0.05），suggesting that the number of effective cases in the treatment group was higher than that in the control group. The average hospitalization days of patients in the treatment group and the control group was （7.3 + 2.4） days and （9.5 + 3.6） days， respectively，there was a statistical significant difference（P < 0.05）. The blood transfusion volumes of patients in the treatment group was less than that in the control group.Conclusion On the basis of octreotide treatment，continuous pumping of 8 milligrams of esomeprazole per minute after intravenous injection of 80 mg of esomeprazole has better hemostatic effect， shorter hospitalization time， and less blood transfusion volume in treatment of nonvariceal upper gastrointestinal bleeding， so it is worthy of clinical promotion.
［Abstract］Objective To investigate the clinical effect of different doses of esomeprazole in treatment of nonvariceal upper gastrointestinal bleeding（ANVUGIB）．Methods We collected the data of 90 patients with ANVUGIB admitted in our hospital from August 2014 to October 2015. The 90 patients were divided into 2 groups: treatment group and control group. Patients in both group were given the combination of Octreotide and other treatment，patients in the treatment group were given continuous pumping of 8 milligrams of esomeprazole per minute for 72 hours after intravenous injection of 80 mg of esomeprazole， patients in the control group were given intravenous instillation of 40 mg of esomeprazole every 12 hours. Then we compared the clinical effect between two groups. Results In the two groups，we statistically analyzed the number of effective cases according to the gender（male and femal），age over 60 years old， and the hemoglobin less than 70.0 grams，there was a statistical significant difference （P < 0.05），suggesting that the number of effective cases in the treatment group was higher than that in the control group. The average hospitalization days of patients in the treatment group and the control group was （7.3 + 2.4） days and （9.5 + 3.6） days， respectively，there was a statistical significant difference（P < 0.05）. The blood transfusion volumes of patients in the treatment group was less than that in the control group.Conclusion On the basis of octreotide treatment，continuous pumping of 8 milligrams of esomeprazole per minute after intravenous injection of 80 mg of esomeprazole has better hemostatic effect， shorter hospitalization time， and less blood transfusion volume in treatment of nonvariceal upper gastrointestinal bleeding， so it is worthy of clinical promotion.
The Clinical Characteristics of Patients with Chronic Heart Failure with Preserved Ejection Fraction
2016, 37(04)
Abstract:
［Abstract］Objective To investigate the difference in the clinical characteristics of patients with chronic heart failure with preserved ejection fraction（HF-PEF），HF-PEF，borderline andreduced ejection fraction （HF-REF）. Methods Two hundred and twenty-two patients with chronic heart failure in our hospital between August 2013 and August 2015 were analysed retrospectively. According to the different LVEF， the patients were divided into HF-PEF group（ n＝81），HF-PEF group，borderline group（n＝71） and HF-REF group（n＝70）. Each patient received examinations of echocardiography， electrocardiogram（ECG），blood biochemistry. And the clinical data of patients were recorded and the clinical characteristics of HF-PEF，HF-PEF，borderline and HF-REF were compared. Results （1）The difference was statistically significant between HF-PEF and HF-REF in patients with age， sex， occupation ratio，smoking history，body mass index（BMI），the basic cause of coronary heart disease， hypertension，atrial fibrillation and heart function classification，systolic blood pressure，and heart rate（P＜0.05）. （2）Compared with patients with HF-REF， E/A（mitral diastolic blood flow velocity / mitral diastolic blood flow velocity），left atrial diameter， pulmonary artery diameter，LVEDD（left ventricular end diastolic diameter） were larger，and LVEF was lower，while the HF-PEF patients had a thicker ventricular septum（P < 0.05）. （3）Compared with the patients with HF-REF， the QRS interval was shorter，Atrial arrhythmia was easy to occur in patients with HF-PEF，but ventricular arrhythmia in patients with HF-REF easy to occur（ P＜0.05）;（4） Compared with HF-REF patients， the levels of serum cholesterol，triglyceride，serum creatinine，blood urea nitrogen， blood urea nitrogen，serum uric acid，C reactive protein and N- terminal brain natriuretic peptide in patients with HF-PEF were statistically different（P＜0.05）. Conclusions In HF-PEF patients，the proportion of women，workers and so on is higher， the elderly patients are more， and the body mass index and blood pressure level is higher. Patients with history of hypertension have higher incidence of atrial fibrillation. HF-PEF patients with concentric hypertrophy have obvious diastolic dysfunction . The evaluation of cardiac function in patients with HF-PEF shows that the proportion of cardiac function grade II is higher and these patients are more prone to atrial arrhythmias.
［Abstract］Objective To investigate the difference in the clinical characteristics of patients with chronic heart failure with preserved ejection fraction（HF-PEF），HF-PEF，borderline andreduced ejection fraction （HF-REF）. Methods Two hundred and twenty-two patients with chronic heart failure in our hospital between August 2013 and August 2015 were analysed retrospectively. According to the different LVEF， the patients were divided into HF-PEF group（ n＝81），HF-PEF group，borderline group（n＝71） and HF-REF group（n＝70）. Each patient received examinations of echocardiography， electrocardiogram（ECG），blood biochemistry. And the clinical data of patients were recorded and the clinical characteristics of HF-PEF，HF-PEF，borderline and HF-REF were compared. Results （1）The difference was statistically significant between HF-PEF and HF-REF in patients with age， sex， occupation ratio，smoking history，body mass index（BMI），the basic cause of coronary heart disease， hypertension，atrial fibrillation and heart function classification，systolic blood pressure，and heart rate（P＜0.05）. （2）Compared with patients with HF-REF， E/A（mitral diastolic blood flow velocity / mitral diastolic blood flow velocity），left atrial diameter， pulmonary artery diameter，LVEDD（left ventricular end diastolic diameter） were larger，and LVEF was lower，while the HF-PEF patients had a thicker ventricular septum（P < 0.05）. （3）Compared with the patients with HF-REF， the QRS interval was shorter，Atrial arrhythmia was easy to occur in patients with HF-PEF，but ventricular arrhythmia in patients with HF-REF easy to occur（ P＜0.05）;（4） Compared with HF-REF patients， the levels of serum cholesterol，triglyceride，serum creatinine，blood urea nitrogen， blood urea nitrogen，serum uric acid，C reactive protein and N- terminal brain natriuretic peptide in patients with HF-PEF were statistically different（P＜0.05）. Conclusions In HF-PEF patients，the proportion of women，workers and so on is higher， the elderly patients are more， and the body mass index and blood pressure level is higher. Patients with history of hypertension have higher incidence of atrial fibrillation. HF-PEF patients with concentric hypertrophy have obvious diastolic dysfunction . The evaluation of cardiac function in patients with HF-PEF shows that the proportion of cardiac function grade II is higher and these patients are more prone to atrial arrhythmias.
2016, 37(04)
Abstract:
［Abstract］Objective To study the clinical application and therapeutic effect of new adjuvant chemotherapy combined with operation treatment and single operation treatment in treatment of stage Ib2-II a locally advanced cervical cancer . Methods Forty patients with stage Ib2-IIa locally advanced cervical cancer treated in our hospital were divided into two groups，respectively treated by different methods. The observation group of 20 cases was gvien the new adjuvant chemotherapy combined with operation therapy；while the control group of 20 cases accepted the direct operation treatment. Then we compared tumor changes，the operation time，intraoperative bleeding volume， the rate of positive margins and metastatic lymph node ratio of patients after chemotherapy between the two groups， and evaluated the clinical application value of new adjuvant chemotherapy combined with operation treatment。 Results After chemotherapy， the patients' tumor size was smaller than that before chemotherapy in the observation group （P＜0.05）， the operation time and bleeding volume was significantly less in the observation group than those of the control group （P＜0.05）， the observation group margin positive rate and the lymph node metastasis positive rate were significantly lower than those of the control group， there was a statistically significant difference between them （P＜0.05）．Conclusions In the treatment of stage Ib2-IIa locally advanced cervical cancer， new adjuvant chemotherapy combined with operation therapy methods is more effective than single operation therapy， and the clinical effect is better，too.
［Abstract］Objective To study the clinical application and therapeutic effect of new adjuvant chemotherapy combined with operation treatment and single operation treatment in treatment of stage Ib2-II a locally advanced cervical cancer . Methods Forty patients with stage Ib2-IIa locally advanced cervical cancer treated in our hospital were divided into two groups，respectively treated by different methods. The observation group of 20 cases was gvien the new adjuvant chemotherapy combined with operation therapy；while the control group of 20 cases accepted the direct operation treatment. Then we compared tumor changes，the operation time，intraoperative bleeding volume， the rate of positive margins and metastatic lymph node ratio of patients after chemotherapy between the two groups， and evaluated the clinical application value of new adjuvant chemotherapy combined with operation treatment。 Results After chemotherapy， the patients' tumor size was smaller than that before chemotherapy in the observation group （P＜0.05）， the operation time and bleeding volume was significantly less in the observation group than those of the control group （P＜0.05）， the observation group margin positive rate and the lymph node metastasis positive rate were significantly lower than those of the control group， there was a statistically significant difference between them （P＜0.05）．Conclusions In the treatment of stage Ib2-IIa locally advanced cervical cancer， new adjuvant chemotherapy combined with operation therapy methods is more effective than single operation therapy， and the clinical effect is better，too.
2016, 37(04)
Abstract:
［Abstract］Objective To study the application of coronary endarterectomy in surgical treatment of coronary heart disease，and to evaluate the early stage effect of CE plus CABG on patients with severe diffuse coronary artery stenosis. Methods Eighteen patients，who underwent coronary endarterectomy in our center and were followed up for 3 months between January 2013 and August 2015，were reviewed retrospectively and compared the group with 18 patients of their LV function，LVEDD，and cardiac function. Results Of all these 18 total coronary endarterectomies，6 cases（33.33%） were left anterior descending artery lesion，4 cases（22.22%）were diagonal branches lesion，4 cases（22.22%） involved the right coronary artery，3cases（16.67%）were posterior descending branches lesion， and 1 case（5.56%） were marginal branches lesion. Afer operation， all the patients recovered well in the early stage，without complications，LVEF and cardiac function improved significantly， LVEDD reduced significantly，compared with themselves preoperativly（P > 0.05）. Conclusion Cornary endarterectomy is an effective treatment for patients with diffuse coronary artery disease. This procedure will improve cardiac function and take a turn for the worse cardiac remodeling for patients with diffuse coronary artery disease.
［Abstract］Objective To study the application of coronary endarterectomy in surgical treatment of coronary heart disease，and to evaluate the early stage effect of CE plus CABG on patients with severe diffuse coronary artery stenosis. Methods Eighteen patients，who underwent coronary endarterectomy in our center and were followed up for 3 months between January 2013 and August 2015，were reviewed retrospectively and compared the group with 18 patients of their LV function，LVEDD，and cardiac function. Results Of all these 18 total coronary endarterectomies，6 cases（33.33%） were left anterior descending artery lesion，4 cases（22.22%）were diagonal branches lesion，4 cases（22.22%） involved the right coronary artery，3cases（16.67%）were posterior descending branches lesion， and 1 case（5.56%） were marginal branches lesion. Afer operation， all the patients recovered well in the early stage，without complications，LVEF and cardiac function improved significantly， LVEDD reduced significantly，compared with themselves preoperativly（P > 0.05）. Conclusion Cornary endarterectomy is an effective treatment for patients with diffuse coronary artery disease. This procedure will improve cardiac function and take a turn for the worse cardiac remodeling for patients with diffuse coronary artery disease.
2016, 37(04)
Abstract:
［Abstract］Objective To investigate the ultrasonic diagnosis and misdiagnosis of permanent right umbilical vein （PRUV）. Methods We retrospectively analyzed the 16 cases of PRUV diagnosed by ultrasound examination and 3 cases of misdiagnosed PRUV in our hospital in recent years．Results The detection rate of PRUV was 0.22% （16/7086）. 13 cases were intrahepatic type， 5 cases were combined with fetal deformity， 3 cases were ultrahepatic type. There were 3 cases of misdiagnosis， including 1 case of fetal PRUV complicated with broken inferior vena cava， and 2 cases of single PRUV. 5 cases of PRUV were combined with fetal deformities， the prognosis of 13 cases of single PRUV was good， 3 cases received induced labor．Conclusions Prenatal ultrasound examination has higher value in the diagnosis of fetal PRUV， but may cause misdiagnosis when the ultrasonography is not typical. The diagnostic rate may be further improved through the analysis of misdiagnosis.
［Abstract］Objective To investigate the ultrasonic diagnosis and misdiagnosis of permanent right umbilical vein （PRUV）. Methods We retrospectively analyzed the 16 cases of PRUV diagnosed by ultrasound examination and 3 cases of misdiagnosed PRUV in our hospital in recent years．Results The detection rate of PRUV was 0.22% （16/7086）. 13 cases were intrahepatic type， 5 cases were combined with fetal deformity， 3 cases were ultrahepatic type. There were 3 cases of misdiagnosis， including 1 case of fetal PRUV complicated with broken inferior vena cava， and 2 cases of single PRUV. 5 cases of PRUV were combined with fetal deformities， the prognosis of 13 cases of single PRUV was good， 3 cases received induced labor．Conclusions Prenatal ultrasound examination has higher value in the diagnosis of fetal PRUV， but may cause misdiagnosis when the ultrasonography is not typical. The diagnostic rate may be further improved through the analysis of misdiagnosis.
2016, 37(04)
Abstract:
［Abstract］Objective To observe the influence of glutathion（GSH）combined entecavir（ETV） on the serum levels of liver fibrosis indexes of patients with chronic hepatitis B（CHB）. Methods We randomly selected 84 cases of patients treated with CHB from February 2012 to March 2014 in our hospital，all the selected patients were randomly divided into 2 groups: the treatment group（GSH combined ETV treatment，n＝43） and the control group （use ETV treatment，n＝41）. The serum liver fibrosis indexes were compared between two groups after treatment. The serum liver fibrosis indexes were compared between pre -and post- treatment in the treatment group．Results（1）After treatment， serum liver fibrosis indexes of the treatment group were significantly lower than those in control group （P < 0.05）， the difference was statistically significant.（2）In the control group， the serum liver fibrosis indexes of post-treatment were significantly lower than those pre-treatment's（P < 0.05）， the difference was statistically significant. Conclusion The combination therapy of GSH and ETV can effectively reduce the serum levels of liver fibrosis indexes and delay the formation process of liver fibrosis.
［Abstract］Objective To observe the influence of glutathion（GSH）combined entecavir（ETV） on the serum levels of liver fibrosis indexes of patients with chronic hepatitis B（CHB）. Methods We randomly selected 84 cases of patients treated with CHB from February 2012 to March 2014 in our hospital，all the selected patients were randomly divided into 2 groups: the treatment group（GSH combined ETV treatment，n＝43） and the control group （use ETV treatment，n＝41）. The serum liver fibrosis indexes were compared between two groups after treatment. The serum liver fibrosis indexes were compared between pre -and post- treatment in the treatment group．Results（1）After treatment， serum liver fibrosis indexes of the treatment group were significantly lower than those in control group （P < 0.05）， the difference was statistically significant.（2）In the control group， the serum liver fibrosis indexes of post-treatment were significantly lower than those pre-treatment's（P < 0.05）， the difference was statistically significant. Conclusion The combination therapy of GSH and ETV can effectively reduce the serum levels of liver fibrosis indexes and delay the formation process of liver fibrosis.
2016, 37(04)
Abstract:
［Abstract］Objective To compare the clinical value of ECLIA and ELISA in the diagnosis of syphilis. Methods Serum samples were collected from 183 patients with syphilis in our hospital，and 861 patients with non syphilis. ELISA method and ECLIA method were used to detect the antibody of syphilis antibody. Differences in sensitivity，specificity and accuracy were compared and analyzed by two methods. Results The sensitivities of methods measured by ECLIA and ELISA were 100% and 99.45%， respectively. The specificity was 99.88% and 100%， respectively. There was no significant difference between ECLIA and ELISA（P＞0.05）. The sensitivity in ECLIA method is higher than that in ELISA method. Conclusions ECLIA method is a sensitive， specific and accurate method for the detection of syphilis antibody，which has the advantages of simple operation， fast detection speed， good result and repeatability.
［Abstract］Objective To compare the clinical value of ECLIA and ELISA in the diagnosis of syphilis. Methods Serum samples were collected from 183 patients with syphilis in our hospital，and 861 patients with non syphilis. ELISA method and ECLIA method were used to detect the antibody of syphilis antibody. Differences in sensitivity，specificity and accuracy were compared and analyzed by two methods. Results The sensitivities of methods measured by ECLIA and ELISA were 100% and 99.45%， respectively. The specificity was 99.88% and 100%， respectively. There was no significant difference between ECLIA and ELISA（P＞0.05）. The sensitivity in ECLIA method is higher than that in ELISA method. Conclusions ECLIA method is a sensitive， specific and accurate method for the detection of syphilis antibody，which has the advantages of simple operation， fast detection speed， good result and repeatability.
2016, 37(04)
Abstract:
［Abstract］Objective To discusse the accuracy of imaging and pathological diagnosis in pulmonary space-occuping lesions. Methods The clinical data of 110 cases of pulmonary space-occuping lesions confirmed by x-ray or CT were retrospectively analyzed. And the intercostal color ultrasound examination was performed to explore the space-occuping lesions of the lung, the biopsy was performed to confirm the diagnosis for the patients without a definitive diagnosis．Results Among the 110 cases of pulmonary space-occuping lesions, there were 100 cases with the consistent results of imaging and pathological examination, accounting for 90.9%，with 95% CI（88.16%～93.64%）；there were 10 cases with different results of imaging and pathological examination，accounting for 9.09%．Conclusions Ultrasound-guided percutaneous biopsy is a lowly risky，minimally invasive，safe and practical examination technology in the diagnosis of peripheral pulmonary space-occuping lesions. Ultrasound-guided percutaneous biopsy combined with pathological diagnosis can eliminate the eroor of the imaging diagnosis，and impove the diagnostic accuracy
［Abstract］Objective To discusse the accuracy of imaging and pathological diagnosis in pulmonary space-occuping lesions. Methods The clinical data of 110 cases of pulmonary space-occuping lesions confirmed by x-ray or CT were retrospectively analyzed. And the intercostal color ultrasound examination was performed to explore the space-occuping lesions of the lung, the biopsy was performed to confirm the diagnosis for the patients without a definitive diagnosis．Results Among the 110 cases of pulmonary space-occuping lesions, there were 100 cases with the consistent results of imaging and pathological examination, accounting for 90.9%，with 95% CI（88.16%～93.64%）；there were 10 cases with different results of imaging and pathological examination，accounting for 9.09%．Conclusions Ultrasound-guided percutaneous biopsy is a lowly risky，minimally invasive，safe and practical examination technology in the diagnosis of peripheral pulmonary space-occuping lesions. Ultrasound-guided percutaneous biopsy combined with pathological diagnosis can eliminate the eroor of the imaging diagnosis，and impove the diagnostic accuracy
2016, 37(04)
Abstract:
［Abstract］Recent studies have shown that the process of organization and recanalization of venous thrombus is similar to the wound healing process， both are associated with the venous wall inflammation，a variety of inflammatory cell infiltration and neovascularization. Remarkably，high concentrations of MCs/Mphs also are detected in the regions of recanalization，both in spontaneous human and in experimental animal thrombi，suppression function of mononuclear macrophages can inhibit thrombus dissolving process. Otherwise，a lot of experimental data suggest a role of blood-borne mononuclear cells，including mononuclear-derived macrophage in organization，vascularization，and recanalization of thrombi. Now this paper will review the mechanism and research progress of MCs/Mphs in promoting Vein Thrombosis organization and recanalization.
［Abstract］Recent studies have shown that the process of organization and recanalization of venous thrombus is similar to the wound healing process， both are associated with the venous wall inflammation，a variety of inflammatory cell infiltration and neovascularization. Remarkably，high concentrations of MCs/Mphs also are detected in the regions of recanalization，both in spontaneous human and in experimental animal thrombi，suppression function of mononuclear macrophages can inhibit thrombus dissolving process. Otherwise，a lot of experimental data suggest a role of blood-borne mononuclear cells，including mononuclear-derived macrophage in organization，vascularization，and recanalization of thrombi. Now this paper will review the mechanism and research progress of MCs/Mphs in promoting Vein Thrombosis organization and recanalization.
2016, 37(04)
Abstract:
［Abstract］The incidence of diabetes is incredibly high, the blindness from diabetes is rising in recent years and the chronic complications are threatening diabetic patients especially for the poor. It is a great burden not only for the patients and their family, but also for the society. We reviewed the recent epidemiological data, analyzed the current situation of prevention and treatment of diabetic eye disease, especially in Yunnan Province.
［Abstract］The incidence of diabetes is incredibly high, the blindness from diabetes is rising in recent years and the chronic complications are threatening diabetic patients especially for the poor. It is a great burden not only for the patients and their family, but also for the society. We reviewed the recent epidemiological data, analyzed the current situation of prevention and treatment of diabetic eye disease, especially in Yunnan Province.
2016, 37(04)
Abstract:
［Abstract］Objective To learn the the overall situation of the first medical and health "college entrance vocational skills test" for three kinds of vocational school students in Yunnan province，analyze the existent problems， and put forward suggestions to solve the problems. Methods We analyzed the college entrance vocational skills test scores of 2912 examinees， calculated the average score and the excellent and good rate of each skill test， and compared the results between public schools and private schools．Results In anatomy test， blood group identification test and blood pressure measurement test， the average score of 2912 examinees was 115.00， 40.59 and 38.37 ， respectively; the excellent and good rate was 23.86%， 65.76% and 54.64%， respectively. The excellent and good rate of the above three tests in private school students were significantly higher than the public school students. Conclusion In the first medical and health "college entrance vocational skills test" for three kinds of vocational school students in Yunnan province，the examinees are weak in identifying human anatomical specimen，while do well in blood test and blood pressure measurement. It is suggested that the administrative departments of education should strengthen the examination and management of the secondary vocational medical and health professional schools，and focus on the practice teaching quality of public health professional schools. Related schools need to increase the proportion of practice teaching， strengthen students' practical ability， pay attention to medical humanities education， and train students' good professional ethics and communication skills.
［Abstract］Objective To learn the the overall situation of the first medical and health "college entrance vocational skills test" for three kinds of vocational school students in Yunnan province，analyze the existent problems， and put forward suggestions to solve the problems. Methods We analyzed the college entrance vocational skills test scores of 2912 examinees， calculated the average score and the excellent and good rate of each skill test， and compared the results between public schools and private schools．Results In anatomy test， blood group identification test and blood pressure measurement test， the average score of 2912 examinees was 115.00， 40.59 and 38.37 ， respectively; the excellent and good rate was 23.86%， 65.76% and 54.64%， respectively. The excellent and good rate of the above three tests in private school students were significantly higher than the public school students. Conclusion In the first medical and health "college entrance vocational skills test" for three kinds of vocational school students in Yunnan province，the examinees are weak in identifying human anatomical specimen，while do well in blood test and blood pressure measurement. It is suggested that the administrative departments of education should strengthen the examination and management of the secondary vocational medical and health professional schools，and focus on the practice teaching quality of public health professional schools. Related schools need to increase the proportion of practice teaching， strengthen students' practical ability， pay attention to medical humanities education， and train students' good professional ethics and communication skills.
2016, 37(04)
Abstract:
［Abstract］Objective To observe the effect of the combination of case teaching and seminar teaching in the clinical practice teaching of Ophthalmology for undergraduates. Methods We selected 2011 grade clinical undergraduates as the research object， class three and class four were selected as the experimental group（114 people） and given the combination of case teaching and seminar teaching. Class five and class six were selected as the control group（112 people）， and given the traditional teaching method. At the end of the course，the students in both groups were given a questionnaire survey and close-book examination，and the results were analyzed by spss19.0 software. Results The questionnaire survey showed there were statistically significant differences in the helping understand the knowledge points， the interest of teaching mode，improving the self-learning ability，and stimulating the creative thinking between the two groups（P＜0.05）. There were also statistically significant differences in the examination result between the two groups（P＜0.05）．Conclusion The method of combining the case teaching and seminar teaching is better than traditional teaching method .
［Abstract］Objective To observe the effect of the combination of case teaching and seminar teaching in the clinical practice teaching of Ophthalmology for undergraduates. Methods We selected 2011 grade clinical undergraduates as the research object， class three and class four were selected as the experimental group（114 people） and given the combination of case teaching and seminar teaching. Class five and class six were selected as the control group（112 people）， and given the traditional teaching method. At the end of the course，the students in both groups were given a questionnaire survey and close-book examination，and the results were analyzed by spss19.0 software. Results The questionnaire survey showed there were statistically significant differences in the helping understand the knowledge points， the interest of teaching mode，improving the self-learning ability，and stimulating the creative thinking between the two groups（P＜0.05）. There were also statistically significant differences in the examination result between the two groups（P＜0.05）．Conclusion The method of combining the case teaching and seminar teaching is better than traditional teaching method .
