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RSS2017 Vol. 38, No. 08
2017, 38(08): 1-5.
Abstract:
Objective To investigate the effects of Corilagin on expression of C-reactive protein (CRP) in oxidized low density lipoprotein ( OX-LDL) injured macrophage cells. Methods In vitro, mice monocyte-derived macrophages were prepared to duplicate the injury model caused by OX-LDL; Western blotting essay was used to determine the effect of Corilagin on CRP protein expression and Real time PCR assay was used to study the CRP m RNA expression, respectively. Results Compared with normal control group, the CRP/β-actin and 2-ΔΔCtvalues of OX-LDL injured model group were markedly increased (P<0.05) ;compared with the model group, Corilagin at 3.125-50 μmol/L obviously decreased the values of CRP/β-actin and 2-ΔΔCt (P<0.05) . Conclusion Corilagin could significantly down-regulate protein and m RNA expression of CRP in OX-LDL injured macrophage cells.
Objective To investigate the effects of Corilagin on expression of C-reactive protein (CRP) in oxidized low density lipoprotein ( OX-LDL) injured macrophage cells. Methods In vitro, mice monocyte-derived macrophages were prepared to duplicate the injury model caused by OX-LDL; Western blotting essay was used to determine the effect of Corilagin on CRP protein expression and Real time PCR assay was used to study the CRP m RNA expression, respectively. Results Compared with normal control group, the CRP/β-actin and 2-ΔΔCtvalues of OX-LDL injured model group were markedly increased (P<0.05) ;compared with the model group, Corilagin at 3.125-50 μmol/L obviously decreased the values of CRP/β-actin and 2-ΔΔCt (P<0.05) . Conclusion Corilagin could significantly down-regulate protein and m RNA expression of CRP in OX-LDL injured macrophage cells.
2017, 38(08): 6-10.
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Objective To study the derivatives of ginsenoside sapogenins and their activities against tumor.Methods The ginsenosides Rg1 and Rb1 were treated with Smith degradation. The products were separated and purified by silica gel column chromatography. The structures of the products were determined by NMR spectra.The activity of anti-tumor cells of compound (1) and compound (2) was detected with CDC25 B activity assay and fluorescence technique.Results 20 (S) -protopanaxadiol (PD) , 20 (S) -protopanaxatriol (PT) , 1'-hydroxy ethanedioxy PT (1) and 1'-hydroxy ethanedioxy PD (2) were isolated and identified. Conclusion During the Smith degradation, we obtained the derivatives of ginsenoside sapogenins. Compound 2 is a novel derivative of ginsenoside sapogenin.Compound 2 is a novel derivative of ginsenoside sapogenin with higher inhibitory activity against cell division cycle 25 homolog B.
Objective To study the derivatives of ginsenoside sapogenins and their activities against tumor.Methods The ginsenosides Rg1 and Rb1 were treated with Smith degradation. The products were separated and purified by silica gel column chromatography. The structures of the products were determined by NMR spectra.The activity of anti-tumor cells of compound (1) and compound (2) was detected with CDC25 B activity assay and fluorescence technique.Results 20 (S) -protopanaxadiol (PD) , 20 (S) -protopanaxatriol (PT) , 1'-hydroxy ethanedioxy PT (1) and 1'-hydroxy ethanedioxy PD (2) were isolated and identified. Conclusion During the Smith degradation, we obtained the derivatives of ginsenoside sapogenins. Compound 2 is a novel derivative of ginsenoside sapogenin.Compound 2 is a novel derivative of ginsenoside sapogenin with higher inhibitory activity against cell division cycle 25 homolog B.
2017, 38(08): 11-15.
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Objective To investigate the possible effects of rosemary on anxiety in rats with multiple concussions. Methods The rat model of three-fold cerebral concussion (3MCC) was performed using a metallic pendulum striker concussive device. Then the rats were randomly divided into six groups:high dose of rosemary intervention group (H) , medium dose of rosemary intervention group (M) , low doses of rosemary intervention group (L) , tween control group (T) , Normal control group (N) , 3MCC injury group (I) . The effect of regular rosemary infusion on anxiety was assessed by open field test. Results (1) Compared with N group: rats of the rest of the groups spent less time in the central area and more time in the surrounding area. The number of total grid, and central grid, however, the number of surrounding grid were less in all groups except M group. (2) Compared with I group:Rats in rosemary intervention groups spent more time in central area and less time in surrounding ares. And the number of total grid in rosemary intervention groups were more. The number of central grid and surrounding grid were obviously more than I group. Moreover, rearing time among all groups have no significant changes. Conclusion The rats can develop anxiety on the 17 th day after three-fold cerebral concussion, and rosemary has a significant anti-anxiety affect on multiple concussions.
Objective To investigate the possible effects of rosemary on anxiety in rats with multiple concussions. Methods The rat model of three-fold cerebral concussion (3MCC) was performed using a metallic pendulum striker concussive device. Then the rats were randomly divided into six groups:high dose of rosemary intervention group (H) , medium dose of rosemary intervention group (M) , low doses of rosemary intervention group (L) , tween control group (T) , Normal control group (N) , 3MCC injury group (I) . The effect of regular rosemary infusion on anxiety was assessed by open field test. Results (1) Compared with N group: rats of the rest of the groups spent less time in the central area and more time in the surrounding area. The number of total grid, and central grid, however, the number of surrounding grid were less in all groups except M group. (2) Compared with I group:Rats in rosemary intervention groups spent more time in central area and less time in surrounding ares. And the number of total grid in rosemary intervention groups were more. The number of central grid and surrounding grid were obviously more than I group. Moreover, rearing time among all groups have no significant changes. Conclusion The rats can develop anxiety on the 17 th day after three-fold cerebral concussion, and rosemary has a significant anti-anxiety affect on multiple concussions.
2017, 38(08): 16-19.
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Objective To isolate the fungal endophytes from Paris Polyphylla Smith var.yunnanensis and investigate the antibacterial activities of these fungi to human's pathogenic bacteria. Methods After surface sterilization, fungal endophytes were cultivated on yeast powder dextrosed (YPD) .The fermentation liquid of every fungal strain was extracted by ethyl acetate. Afterwards, the effect of antibacterial activity on Staphylococcus aureus, Salmonella typhi and Endococcus faecium was investigated by paper disk test. Results54 strains of fungal endophytes were isolated and purified. Among them, 20 strains had antibacterial activities, which accounted for 37% of the total isolated strains. Inhibition zones of J09 metabolites to the three indicators was ( 49.67 ±1.25) mm, ( 50.33 ±0.52) mm, ( 37.00 ±1.63) mm respectively. In terms of the morphological and molecular biological characteristics, J09 was identified as Penicillium griseofulvum.Meanwhile, the antibacterial compounds were not penicillin or griseofulvin by high performance liquid chromatography (HPLC) detection. Conclusion The fungal endophyte J09 could be a potential candidate to develop an antibacterial medicine since its metabolites have showed strong antibacterial activities.
Objective To isolate the fungal endophytes from Paris Polyphylla Smith var.yunnanensis and investigate the antibacterial activities of these fungi to human's pathogenic bacteria. Methods After surface sterilization, fungal endophytes were cultivated on yeast powder dextrosed (YPD) .The fermentation liquid of every fungal strain was extracted by ethyl acetate. Afterwards, the effect of antibacterial activity on Staphylococcus aureus, Salmonella typhi and Endococcus faecium was investigated by paper disk test. Results54 strains of fungal endophytes were isolated and purified. Among them, 20 strains had antibacterial activities, which accounted for 37% of the total isolated strains. Inhibition zones of J09 metabolites to the three indicators was ( 49.67 ±1.25) mm, ( 50.33 ±0.52) mm, ( 37.00 ±1.63) mm respectively. In terms of the morphological and molecular biological characteristics, J09 was identified as Penicillium griseofulvum.Meanwhile, the antibacterial compounds were not penicillin or griseofulvin by high performance liquid chromatography (HPLC) detection. Conclusion The fungal endophyte J09 could be a potential candidate to develop an antibacterial medicine since its metabolites have showed strong antibacterial activities.
2017, 38(08): 20-24.
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Objective To summarize the normal reference values of atrioventricular diameter and cardiac function parameters in Kunming local healthy population by magnetic resonance imaging (MRI) . Methods The 1.5T magnetic resonance scanner was used routinely to link the vector gating and according to a rapid steady-state balance precession sequence was applied to perform a cardiac cine scan in 97 Kunming local healthy volunteers. The acquisition of cardiac cine images included the left ventricular horizontal long axis (HLA) , the left ventricular vertical long axis (VLA) , the left ventricular short axis (SA) , the left and right atrial axial.Following the scan, AW Report CARD 4.4 heart specific analysis software was used to measure the linear length of image and measure the volume of the image. The measured values were statistically processed to obtain the mean parameters of the heart, atrioventricular and cardiac functions. Non-Paired T tests were performed for male and female corresponding parameters. Meanwhile the relationship between these corresponding parameters and UCG was researched. Results All volunteers received high-quality heart tomographic images and the sampling points were legible. Multiple parameters of cardiac atrioventricular line and cardiac function were obtained, according to heart tomographic images. All of the above cardiac atrioventricular values were positively correlated with UCG measurements. No significant association was found in left and right ventricular ejection fraction (P>0.05) , cardiac index (P >0.05) and sex (P >0.05) . However, all the other indexes were statistically significant (P<0.05) . The results also showed that the thickness of the left ventricular anterior chamber, the left ventricular posterior wall thickness, the left ventricular ejection fraction, and the right ventricular ejection fraction were slightly higher than those in the plain region (P <0.05) .Conclusion Heart magnetic resonance scanning can obtain reliable atrioventricular line and ventricular systolic function in Kunming local healthy population.
Objective To summarize the normal reference values of atrioventricular diameter and cardiac function parameters in Kunming local healthy population by magnetic resonance imaging (MRI) . Methods The 1.5T magnetic resonance scanner was used routinely to link the vector gating and according to a rapid steady-state balance precession sequence was applied to perform a cardiac cine scan in 97 Kunming local healthy volunteers. The acquisition of cardiac cine images included the left ventricular horizontal long axis (HLA) , the left ventricular vertical long axis (VLA) , the left ventricular short axis (SA) , the left and right atrial axial.Following the scan, AW Report CARD 4.4 heart specific analysis software was used to measure the linear length of image and measure the volume of the image. The measured values were statistically processed to obtain the mean parameters of the heart, atrioventricular and cardiac functions. Non-Paired T tests were performed for male and female corresponding parameters. Meanwhile the relationship between these corresponding parameters and UCG was researched. Results All volunteers received high-quality heart tomographic images and the sampling points were legible. Multiple parameters of cardiac atrioventricular line and cardiac function were obtained, according to heart tomographic images. All of the above cardiac atrioventricular values were positively correlated with UCG measurements. No significant association was found in left and right ventricular ejection fraction (P>0.05) , cardiac index (P >0.05) and sex (P >0.05) . However, all the other indexes were statistically significant (P<0.05) . The results also showed that the thickness of the left ventricular anterior chamber, the left ventricular posterior wall thickness, the left ventricular ejection fraction, and the right ventricular ejection fraction were slightly higher than those in the plain region (P <0.05) .Conclusion Heart magnetic resonance scanning can obtain reliable atrioventricular line and ventricular systolic function in Kunming local healthy population.
2017, 38(08): 25-29.
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Objective To investigate the genetic polymorphisms of 27 Y-STR loci in Hui population from Weishan, Yunnan Province, and obtain the genetic basic data of Hui population, to provide data support for forensic identification, and to promote the establishment of Y-STR database. Methods Blood samples of 171 unrelated male individuals were collected.DNA was amplified by using YfilerRPlus system and genotyped by ABI3500 Genetic Analyzer. The forensic parameters were calculated.Results In the 171 unrelated male individuals, the allele frequency was 0.00580.7207, the gene diversity (GD) was 0.43140.9408. 169 different haplotypes were observed once, 1 haplotype occurred twice.The haplotype diversity (HD) was0.99986 and the discriminative capacity (DC) was 0.994152.Conclusion The 27 Y-STR loci are highly polymorphic in Hui population from Weishan, Yunnan Province, and could be provided the basis for forensic identification and the establishment of Y-STR database.
Objective To investigate the genetic polymorphisms of 27 Y-STR loci in Hui population from Weishan, Yunnan Province, and obtain the genetic basic data of Hui population, to provide data support for forensic identification, and to promote the establishment of Y-STR database. Methods Blood samples of 171 unrelated male individuals were collected.DNA was amplified by using YfilerRPlus system and genotyped by ABI3500 Genetic Analyzer. The forensic parameters were calculated.Results In the 171 unrelated male individuals, the allele frequency was 0.00580.7207, the gene diversity (GD) was 0.43140.9408. 169 different haplotypes were observed once, 1 haplotype occurred twice.The haplotype diversity (HD) was0.99986 and the discriminative capacity (DC) was 0.994152.Conclusion The 27 Y-STR loci are highly polymorphic in Hui population from Weishan, Yunnan Province, and could be provided the basis for forensic identification and the establishment of Y-STR database.
2017, 38(08): 30-34.
Abstract:
Objective To learn the status of the prevalence and distribution characteristics of congenital heart disease (CHD) of adolescents between 3 to 18 ages among different regions in Yunnan. Methods From January 2013 to November 2014, the epidemic survey of CHD was carried out in regions of Lijiang, Diqing and Qiaojia by cluster sampling.Results The total prevalence rate of CHD was 7.65‰ in 34349 adolescents investigated in this research. However, the morbidity rate in female patients was 8.71‰, and male 6.72‰, the difference had statistical significance (χ2=4.338, P=0.037) ; the difference among different age groups had statistical significance (χ2 =9.622, P =0.022) , and 3 7 age group was the highest 11.94‰; the difference among three regions, Lijiang, Diqing, Qiaojia (6.13‰, 8.09‰, 7.68‰ respectively) had no statistical significance (χ2 =1.761, P =0.415) ; the difference among ethnic groups, the Han, Tibetan, Naxi, Lisu, Yi nationality and the others, had statistical significance (χ2 =14.374, P =0.013) .Conclusions The prevalence of CHD in investigated regions is higher than the average level of the nationwide, is obviously higher in female group than male group, is the highest in the group of 3 7 age, the difference among three regions has no statistical significance. The main kind of CHD is atrial septal defect (ASD) .
Objective To learn the status of the prevalence and distribution characteristics of congenital heart disease (CHD) of adolescents between 3 to 18 ages among different regions in Yunnan. Methods From January 2013 to November 2014, the epidemic survey of CHD was carried out in regions of Lijiang, Diqing and Qiaojia by cluster sampling.Results The total prevalence rate of CHD was 7.65‰ in 34349 adolescents investigated in this research. However, the morbidity rate in female patients was 8.71‰, and male 6.72‰, the difference had statistical significance (χ2=4.338, P=0.037) ; the difference among different age groups had statistical significance (χ2 =9.622, P =0.022) , and 3 7 age group was the highest 11.94‰; the difference among three regions, Lijiang, Diqing, Qiaojia (6.13‰, 8.09‰, 7.68‰ respectively) had no statistical significance (χ2 =1.761, P =0.415) ; the difference among ethnic groups, the Han, Tibetan, Naxi, Lisu, Yi nationality and the others, had statistical significance (χ2 =14.374, P =0.013) .Conclusions The prevalence of CHD in investigated regions is higher than the average level of the nationwide, is obviously higher in female group than male group, is the highest in the group of 3 7 age, the difference among three regions has no statistical significance. The main kind of CHD is atrial septal defect (ASD) .
2017, 38(08): 35-39.
Abstract:
目的 研究云南省晋宁县彝族人群CX3CR1基因T280M多态性分布的情况, 为探讨CX3CR1基因相关性疾病的发病机制奠定基础.方法 采用PCR-RFLP技术对居住在云南省晋宁县268例彝族健康个体的CX3CR1基因T280M多态性进行分析, 计算MM、MT、TT基因型频率和M、T等位基因频率, 与其他人群该位点多态性进行比较.结果 云南省晋宁县彝族CX3CR1基因的MM、MT、TT基因型频率分别为1.12%、11.94%和86.94%, M和T等位基因频率分别为7.09%和92.91%.与汉族、维吾尔族、希腊、法国、塞尔维亚、印度、意大利和高加索人群比较, 无论是基因型分布还是基因频率差异有统计学意义 (P<0.05) p="">0.05) .结论 CX3CR1基因T280M多态性分布存在民族、种族差异, 且种族间的差异大于民族间的差异.
2017, 38(08): 40-43.
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Objective To study the safety and effectiveness of Ma Xiong Shu Tong Fang for migraine and evaluate the white matter lesions (WML) by brain MRI.Methods A total of 122 consecutives were randomly divided into control and observation groups and each group had 61 cases.The patients in control group received Flunarizine Hydrochloride Capsules 10 mg/d, and the patients in observation group adopted Ma Xiong Shu Tong Fang three particles per day, and the course was four weeks. Then we compared TCM syndrome integrals, total effective rate and incidence of adverse reactions, serum 5-HT, NF-κB, IL-6 and TNF-αlevels, positive rate and degrees of WML by MRI. Results The TCM syndrome integrals after treatment in observation group was significantly lower than control group (P <0.05) . There were no severe complications in the two groups. The serum 5-HT, NF-κB, IL-6 and TNF-αlevels in observation group were all lower, positive rate of WML was also lower (P<0.05) . Conclusion Ma Xiong Shu Tong Fang is safe and effective for mgraine, it can reduce serum levels of 5-HT, NF-κB, IL-6 and TNF-αand improve WML.
Objective To study the safety and effectiveness of Ma Xiong Shu Tong Fang for migraine and evaluate the white matter lesions (WML) by brain MRI.Methods A total of 122 consecutives were randomly divided into control and observation groups and each group had 61 cases.The patients in control group received Flunarizine Hydrochloride Capsules 10 mg/d, and the patients in observation group adopted Ma Xiong Shu Tong Fang three particles per day, and the course was four weeks. Then we compared TCM syndrome integrals, total effective rate and incidence of adverse reactions, serum 5-HT, NF-κB, IL-6 and TNF-αlevels, positive rate and degrees of WML by MRI. Results The TCM syndrome integrals after treatment in observation group was significantly lower than control group (P <0.05) . There were no severe complications in the two groups. The serum 5-HT, NF-κB, IL-6 and TNF-αlevels in observation group were all lower, positive rate of WML was also lower (P<0.05) . Conclusion Ma Xiong Shu Tong Fang is safe and effective for mgraine, it can reduce serum levels of 5-HT, NF-κB, IL-6 and TNF-αand improve WML.
2017, 38(08): 44-47.
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Objective To analyze the necessity and clinical application of laryngeal mask airway anesthesia in laparoscopic surgery through comparing the efficacy of laryngeal mask airway and endotracheal intubation in laparoscopic surgery. Methods We selected 76 patients undergoing laparoscopic surgery in the Department of Anesthesiologyin Qujing Second People's Hospital From January 2013 to December. According to the random number table, the patients were divided into tracheal intubation group (n =38) and laryngeal mask group (n=38) . Tracheal intubation group was given disposable tracheal catheter, laryngeal mask group was given I-GEL laryngeal mask. Then the patients in the 2 groups were observed and compared in 6 points of HR, map, PETCO2, recovery time and postoperative restlessness, extubation cough, cough after surgery, the incidence of adverse reactions and hoarseness and sore throat, such as before induction of anesthesia (T1) , immediate intubation (cover) (T2) , pneumoperitoneum (T3) , at 10 min after pneumoperitoneum (T4) , pneumoperitoneum (T5) , stop extubation immediately (cover) (T6) . Results The HR and MAP values in laryngeal mask group at each time point was significantly lower than those in tracheal intubation group, there was no significant difference in PETCO2 between the 2 groups of patients (P >0.05) .The recovery time of patients in laryngeal mask group was (5.12±3.82) min, significantly shorter than that in the intubation group which was (10.14±4.15) min (P <0.05) . The incidence of adverse reaction in I-GEL laryngeal mask group was 5.26%, The incidence of adverse reactions in the tracheal intubation group was 23.68% (P <0.05) .Conclusion During laparoscopic anesthesia with I-GEL laryngeal mask airway, patients have stable hemodynamics in induction and recovery period, short postoperative recovery time, and low incidence of adverse reactions such as postoperative restlessness, extubation cough, postoperative cough, sore throat and hoarseness. The ventilation condition of patients during the operation is good and I-GEL laryngeal mask airway has higher safety, comfort and satisfaction degree, so it has good application value and deserves promoting.
Objective To analyze the necessity and clinical application of laryngeal mask airway anesthesia in laparoscopic surgery through comparing the efficacy of laryngeal mask airway and endotracheal intubation in laparoscopic surgery. Methods We selected 76 patients undergoing laparoscopic surgery in the Department of Anesthesiologyin Qujing Second People's Hospital From January 2013 to December. According to the random number table, the patients were divided into tracheal intubation group (n =38) and laryngeal mask group (n=38) . Tracheal intubation group was given disposable tracheal catheter, laryngeal mask group was given I-GEL laryngeal mask. Then the patients in the 2 groups were observed and compared in 6 points of HR, map, PETCO2, recovery time and postoperative restlessness, extubation cough, cough after surgery, the incidence of adverse reactions and hoarseness and sore throat, such as before induction of anesthesia (T1) , immediate intubation (cover) (T2) , pneumoperitoneum (T3) , at 10 min after pneumoperitoneum (T4) , pneumoperitoneum (T5) , stop extubation immediately (cover) (T6) . Results The HR and MAP values in laryngeal mask group at each time point was significantly lower than those in tracheal intubation group, there was no significant difference in PETCO2 between the 2 groups of patients (P >0.05) .The recovery time of patients in laryngeal mask group was (5.12±3.82) min, significantly shorter than that in the intubation group which was (10.14±4.15) min (P <0.05) . The incidence of adverse reaction in I-GEL laryngeal mask group was 5.26%, The incidence of adverse reactions in the tracheal intubation group was 23.68% (P <0.05) .Conclusion During laparoscopic anesthesia with I-GEL laryngeal mask airway, patients have stable hemodynamics in induction and recovery period, short postoperative recovery time, and low incidence of adverse reactions such as postoperative restlessness, extubation cough, postoperative cough, sore throat and hoarseness. The ventilation condition of patients during the operation is good and I-GEL laryngeal mask airway has higher safety, comfort and satisfaction degree, so it has good application value and deserves promoting.
2017, 38(08): 48-52.
Abstract:
Objective To evaluate the operational methods and therapeutic efficacy of minimally invasive operation with neuroendoscopy and stereotactic techniques in treatment of neurocysticercosis. Methods The clinical characteristics and imaging appearances of 19 patients with neurocysticercosis received minimally invasive operational treatment with neuroendoscopy and Stereotactic techniques were analyzed and summaried retrospectively, 9 cases of neurocysticercosis were lonely extirpated by neuroendoscopy, 7 cases were completed under the guide of stereotactic techniques, 2 cases of septum pellucidum fenestration and ventriculoperitoneal shunt. Results Among 19 cases of patients, there were 17 cases of total resection, 2 cases of unsuccessful removal of third ventricle cysticercus because of hemorrhage during operation or Cysticercus movement to the posterior of Lateral Ventricles. All patients were followed up for 2-60 months, in all cases, no new neurological function defect was found, 16 cases of preoperative symptoms disappeared, 3 cases of epileptiform EEG, 1 case of grand mal, 1 case of partial seizure disorder. Conclusion Minimally invasive treatment with neuroendoscopy and stereotactic techniques for neurocysticercosis has smaller trauma and definite curative effect.
Objective To evaluate the operational methods and therapeutic efficacy of minimally invasive operation with neuroendoscopy and stereotactic techniques in treatment of neurocysticercosis. Methods The clinical characteristics and imaging appearances of 19 patients with neurocysticercosis received minimally invasive operational treatment with neuroendoscopy and Stereotactic techniques were analyzed and summaried retrospectively, 9 cases of neurocysticercosis were lonely extirpated by neuroendoscopy, 7 cases were completed under the guide of stereotactic techniques, 2 cases of septum pellucidum fenestration and ventriculoperitoneal shunt. Results Among 19 cases of patients, there were 17 cases of total resection, 2 cases of unsuccessful removal of third ventricle cysticercus because of hemorrhage during operation or Cysticercus movement to the posterior of Lateral Ventricles. All patients were followed up for 2-60 months, in all cases, no new neurological function defect was found, 16 cases of preoperative symptoms disappeared, 3 cases of epileptiform EEG, 1 case of grand mal, 1 case of partial seizure disorder. Conclusion Minimally invasive treatment with neuroendoscopy and stereotactic techniques for neurocysticercosis has smaller trauma and definite curative effect.
2017, 38(08): 53-57.
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Objective To further understand the genetic characteristics and the outcome of Familial essential tremor.Methods Clinical summary and statistical analysis were performed on the onset age, course of disease of ET, whether it is related to tremor amplitude, drug sensitivity and complication of 39 patients in 7families. Results The correlation coefficient between tremor amplitude and age at onset was rs =0.542, Correlation coefficient of tremor amplitude and duration rs =0.168; The effective rates of oral propranolol in young middle-aged group and elderly group were 79.2% and 35.7%, P=0.014; The effective rates of drinking test were 66.7% and 28.6%, P = 0.042, respectively, in young middle-aged group and elderly group;The incidence of complications in young middle-aged group and elderly group was 8.3% and 42.9%, respectivdy P =0.042.Conclusions With the growth of onset age, hearing loss and cognitive decline and other complications increase significantly; With the duration of the disease, tremor increases amplitude, drug sensitivity gradually decreases.It suggests that ET is easy to transform to other degenerative diseases, also easy to merge other degenerative diseases, This change has a certain relevance with age and heredity. Is is rather than a simple benign disease.
Objective To further understand the genetic characteristics and the outcome of Familial essential tremor.Methods Clinical summary and statistical analysis were performed on the onset age, course of disease of ET, whether it is related to tremor amplitude, drug sensitivity and complication of 39 patients in 7families. Results The correlation coefficient between tremor amplitude and age at onset was rs =0.542, Correlation coefficient of tremor amplitude and duration rs =0.168; The effective rates of oral propranolol in young middle-aged group and elderly group were 79.2% and 35.7%, P=0.014; The effective rates of drinking test were 66.7% and 28.6%, P = 0.042, respectively, in young middle-aged group and elderly group;The incidence of complications in young middle-aged group and elderly group was 8.3% and 42.9%, respectivdy P =0.042.Conclusions With the growth of onset age, hearing loss and cognitive decline and other complications increase significantly; With the duration of the disease, tremor increases amplitude, drug sensitivity gradually decreases.It suggests that ET is easy to transform to other degenerative diseases, also easy to merge other degenerative diseases, This change has a certain relevance with age and heredity. Is is rather than a simple benign disease.
2017, 38(08): 58-61.
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Objective To evaluate the effect of Kinesio Taping on pain in treatment of acute fibrositis Methods 56 patients with acute fibrositis were randomly divided into Kinesio Taping treatment group (n=28) and rehabilitation therapy control group (n=28) . We used visual analogue scale (VAS) and global rating of change scales (GRCS) to evaluate effect follow treatment for 3 days and 6 days. The effect of treatment groups respectively compared using χ2test. Results After 3 days and 6 days, VAS scores of patients in treatment group had greater improvement than those in control group. The difference was statistically significant (P<0.05) . The GCRS score demonstrated that there are 63.85% patients had morn then 3 points after 3 days KT treatment. And96.5%patient score had more than 3 points after 6 days KT treatment, of which there are 67.85% subjects score above 5 points. And these results had statistically significant (P <0.05) . Conclusion Kinesio Taping in treatment of acute fibrositis has a good therapeutic effect, and is worthy of promotion.
Objective To evaluate the effect of Kinesio Taping on pain in treatment of acute fibrositis Methods 56 patients with acute fibrositis were randomly divided into Kinesio Taping treatment group (n=28) and rehabilitation therapy control group (n=28) . We used visual analogue scale (VAS) and global rating of change scales (GRCS) to evaluate effect follow treatment for 3 days and 6 days. The effect of treatment groups respectively compared using χ2test. Results After 3 days and 6 days, VAS scores of patients in treatment group had greater improvement than those in control group. The difference was statistically significant (P<0.05) . The GCRS score demonstrated that there are 63.85% patients had morn then 3 points after 3 days KT treatment. And96.5%patient score had more than 3 points after 6 days KT treatment, of which there are 67.85% subjects score above 5 points. And these results had statistically significant (P <0.05) . Conclusion Kinesio Taping in treatment of acute fibrositis has a good therapeutic effect, and is worthy of promotion.
2017, 38(08): 62-66.
Abstract:
Objective To study the significance of thyroid transcription factor-1 (TTF-1) and phosphatase and tensin homolog (PTEN) genes expression in early endometrial cancer. Methods A total of 41 consecutives as endometrial cancer, 38 patients with hyperplastic endometrium and 13 cases of normal endometrium were enrolled, then we detected the levels of TTF-1 and PTEN mRNA in the three endometrium tissues by fluorescent quantitative PCR (RT-PCR) , and analyzed the relationship between them and clinicopathological features;the correlation of TTF-1 and PTEN mRNA to mi R-135 b, mi R-125 b and Snail mRNA levels in the three endometrium tissues by RT-PCR. Results The levels of TTF-1 and PTEN mRNA in endometrial cancer tissue were both significantly lower than the other two, and were the highest in normal endometrium (P<0.05) .The expressions of TTF-1 and PTEN were no relationship to menopause, adenocarcinoma or squamous cell carcinoma (P >0.05) ; while they were lower as FIGO classes, myometrial invasion and pelvic lymph node metastasis were increasing (P <0.05) .The level of mi R-135 b mRNA in endometrial cancer tissue was significantly higher, while mi R-125 b and Snail mRNA were lower than the other two, mi R-135 b mRNA level in normal endometrium was the lowest, mi R-125 b and Snail mRNA was the highest (P<0.05) .The TTF-1 and PTEN mRNA expressions were positively ralted to mi R-135 b mRNA level, while negatively related to mi R-125 b and Snail mRNA.The diagnosed sensitivity of TTF-1 mRNA for endometrial cancer was 86.5%, specificity84.2%, accuracy 0.823, 95% CI=0.7620.921, P=0.012 by ROC model;while they were 85.3%, 83.6%, 0.842, 95% CI=0.7850.936, P=0.010 of PTEN mRNA correspondingly. Conclusion TTF-1 and PTEN genes can be the molecular markers for early diagnosis of endometrial cancer, are related to clinical features, and may influence tumor by regulating tumor cell proliferation activity.
Objective To study the significance of thyroid transcription factor-1 (TTF-1) and phosphatase and tensin homolog (PTEN) genes expression in early endometrial cancer. Methods A total of 41 consecutives as endometrial cancer, 38 patients with hyperplastic endometrium and 13 cases of normal endometrium were enrolled, then we detected the levels of TTF-1 and PTEN mRNA in the three endometrium tissues by fluorescent quantitative PCR (RT-PCR) , and analyzed the relationship between them and clinicopathological features;the correlation of TTF-1 and PTEN mRNA to mi R-135 b, mi R-125 b and Snail mRNA levels in the three endometrium tissues by RT-PCR. Results The levels of TTF-1 and PTEN mRNA in endometrial cancer tissue were both significantly lower than the other two, and were the highest in normal endometrium (P<0.05) .The expressions of TTF-1 and PTEN were no relationship to menopause, adenocarcinoma or squamous cell carcinoma (P >0.05) ; while they were lower as FIGO classes, myometrial invasion and pelvic lymph node metastasis were increasing (P <0.05) .The level of mi R-135 b mRNA in endometrial cancer tissue was significantly higher, while mi R-125 b and Snail mRNA were lower than the other two, mi R-135 b mRNA level in normal endometrium was the lowest, mi R-125 b and Snail mRNA was the highest (P<0.05) .The TTF-1 and PTEN mRNA expressions were positively ralted to mi R-135 b mRNA level, while negatively related to mi R-125 b and Snail mRNA.The diagnosed sensitivity of TTF-1 mRNA for endometrial cancer was 86.5%, specificity84.2%, accuracy 0.823, 95% CI=0.7620.921, P=0.012 by ROC model;while they were 85.3%, 83.6%, 0.842, 95% CI=0.7850.936, P=0.010 of PTEN mRNA correspondingly. Conclusion TTF-1 and PTEN genes can be the molecular markers for early diagnosis of endometrial cancer, are related to clinical features, and may influence tumor by regulating tumor cell proliferation activity.
2017, 38(08): 67-72.
Abstract:
Objectives To detect the expression levels of IL-17 and IL-23 in peripheral blood of B-cell non-Hodgkin lymphoma patients treated by RCHOP and to study the role of IL-17 and IL-23 in NHL.Methods Twenty-five patients with B-cell NHL who achieved remission after 6 to 8 cycles of R-CHOP as a NHL group, 20 healthy volunteers were recruited as a normal control group.RT-PCR was used to detect the expression levels of IL-17 m RNA and IL-23 m RNA in NHL patients and health volunteers.Results The expression levels of IL-17 m RNA and IL-23 m RNA in the patients with NHL before therapy and the patients with NHL who achieved remission were lower than that in the normal control group (P<0.05) .The expression levels of IL-17 m RNA and IL-23 m RNA in the patients with NHL who achieved remission were higher than that in the patients with NHL before therapy (P=0.001, P<0.05) .Conclusions IL-17 and IL-23 m RNA levels are higher after treated with R-CHOP. The expression levels of IL-17 m RNA and IL-23 m RNA in NHL patients are related with prognosis and efficacy.
Objectives To detect the expression levels of IL-17 and IL-23 in peripheral blood of B-cell non-Hodgkin lymphoma patients treated by RCHOP and to study the role of IL-17 and IL-23 in NHL.Methods Twenty-five patients with B-cell NHL who achieved remission after 6 to 8 cycles of R-CHOP as a NHL group, 20 healthy volunteers were recruited as a normal control group.RT-PCR was used to detect the expression levels of IL-17 m RNA and IL-23 m RNA in NHL patients and health volunteers.Results The expression levels of IL-17 m RNA and IL-23 m RNA in the patients with NHL before therapy and the patients with NHL who achieved remission were lower than that in the normal control group (P<0.05) .The expression levels of IL-17 m RNA and IL-23 m RNA in the patients with NHL who achieved remission were higher than that in the patients with NHL before therapy (P=0.001, P<0.05) .Conclusions IL-17 and IL-23 m RNA levels are higher after treated with R-CHOP. The expression levels of IL-17 m RNA and IL-23 m RNA in NHL patients are related with prognosis and efficacy.
2017, 38(08): 73-78.
Abstract:
Objective To investigate the effect of Yunnan Baiyao on the secretion of TNF-α, IL-6, IL-1βfrom human dental pulp cells ( h DPCs) under lipopolysaccharide from Porphyromonas gingivalis (LPS-PG) induced inflammatory environment, and investigate the inhibition effect of Yunnan Baiyao on pulpitis. Methods HDPCs were cultured by the modified tissue explant culture technique. Cultured h DPCs were identified and verified by the immunocytochemical staining. HDPCs were exposed to 10 μg/m L LPS-PG solution for 1 day, then the LPS-PG containing culture media were removed and collected, the h DPCs were divided into3 groups cultured with different new culture media, the control group was cultured with 1% FBS MEM, the LPS-PG group was cultured with 10 μg/m L LPS-PG, the Baiyao group was cultured with 10μg/m L Yunnan Baiyao. Culturing media were collected on exposure finishing, 1st and 3rd day after the medium changing for detection of IL-6, IL-1β and TNF-αproteins by ELISA, and on 3rd day the quantitative Real-Time PCR (q RT-PCR) was performed for IL-6, IL-1β and TNF-α gene expression of the h DPCs.Results HDPCs culturing showed a positive vimentin expression and a negative keratin expression. 3 groups showed no difference in TNF-α, IL-6 and IL-1β secretions immediately after the LPS-PG exposure for 1 day by ELISA, and the LPS-PG group showed higher secretions of TNF-α, IL-6 and IL-1β than other groups on1 st and 3rd day (P<0.05) . Baiyao group and control group showed no differences (P>0.05) . However, q RT-PCR showed lower gene expressions of TNF-α, IL-6, IL-1β in the Baiyao group than other groups at 3rd day (P <0.05) .Conclusion Under inflammatory environment induced by LPS-PG, the Yunnan Baiyao shows no inhibitory effect on secretion of TNF-α, IL-6 and IL-1β on3 rd day after the medium changing, but can downregulate the m RNA expression.
Objective To investigate the effect of Yunnan Baiyao on the secretion of TNF-α, IL-6, IL-1βfrom human dental pulp cells ( h DPCs) under lipopolysaccharide from Porphyromonas gingivalis (LPS-PG) induced inflammatory environment, and investigate the inhibition effect of Yunnan Baiyao on pulpitis. Methods HDPCs were cultured by the modified tissue explant culture technique. Cultured h DPCs were identified and verified by the immunocytochemical staining. HDPCs were exposed to 10 μg/m L LPS-PG solution for 1 day, then the LPS-PG containing culture media were removed and collected, the h DPCs were divided into3 groups cultured with different new culture media, the control group was cultured with 1% FBS MEM, the LPS-PG group was cultured with 10 μg/m L LPS-PG, the Baiyao group was cultured with 10μg/m L Yunnan Baiyao. Culturing media were collected on exposure finishing, 1st and 3rd day after the medium changing for detection of IL-6, IL-1β and TNF-αproteins by ELISA, and on 3rd day the quantitative Real-Time PCR (q RT-PCR) was performed for IL-6, IL-1β and TNF-α gene expression of the h DPCs.Results HDPCs culturing showed a positive vimentin expression and a negative keratin expression. 3 groups showed no difference in TNF-α, IL-6 and IL-1β secretions immediately after the LPS-PG exposure for 1 day by ELISA, and the LPS-PG group showed higher secretions of TNF-α, IL-6 and IL-1β than other groups on1 st and 3rd day (P<0.05) . Baiyao group and control group showed no differences (P>0.05) . However, q RT-PCR showed lower gene expressions of TNF-α, IL-6, IL-1β in the Baiyao group than other groups at 3rd day (P <0.05) .Conclusion Under inflammatory environment induced by LPS-PG, the Yunnan Baiyao shows no inhibitory effect on secretion of TNF-α, IL-6 and IL-1β on3 rd day after the medium changing, but can downregulate the m RNA expression.
2017, 38(08): 79-85.
Abstract:
Objetive To investgate the association of Vitamin D receptor gene ApaⅠ, BsmⅠ single nucleotide polymorphism with diabetic kidney disease in type 2 diabetic patients.Methods The single nucleotide polymorphism of VDR gene in 235 type 2 diabetes [including 82 normal albuminuria (DKD0 group) , 66microalbuminuria (DKDl group) and 87 macroalbuminuria (DKD2 group) ]and 86 normal controls were detected by Taq Man fluorescent probe. The genotype frequency and allele frequency and relative clinical datawere compared among groups. Results (1) BsmⅠ GG, GA, AA genotype frequencies were 0.931, 0.066, 0.003, allele G and allele G frequencies were 0.964 and 0.036. ApaⅠ CC、CA、AA genotype frequencies were0.536, 0.371, 0.093, allele C and allele A frequencies were 0.721 and 0.279. (2) ApaⅠ allele C frequencies (P<0.05) in DKD2 group (0.770) were significantly higher than in DKD0 group (0.671) , and the allele A frequencies (P<0.05) in DKD2 group (0.230) were significantly lower than in DKD0 group (0.329) (P<0.05) . (3) Logistic analysis showed that BsmⅠ GG genotype (OR = 0.159, P<0.05) was the independent protective factors of DKD occurrence, but it was not related to T2 DKD development. ApaⅠ single nucleotide polymorphism was not related to T2 DKD occurrence and development ( P >0.05) . 25 ( OH) D deficiency/insufficiency may b e the independent risk factor of T2 DKD development (OR = 1.957, P <0.05) .Conclusions BsmⅠ GG genotype is an independent protective factor of T2 DKDoccurrence, but it is not related to T2 DKD development.ApaⅠ singlenucleotide polymorphism is not related to T2 DKD occurrence and development.
Objetive To investgate the association of Vitamin D receptor gene ApaⅠ, BsmⅠ single nucleotide polymorphism with diabetic kidney disease in type 2 diabetic patients.Methods The single nucleotide polymorphism of VDR gene in 235 type 2 diabetes [including 82 normal albuminuria (DKD0 group) , 66microalbuminuria (DKDl group) and 87 macroalbuminuria (DKD2 group) ]and 86 normal controls were detected by Taq Man fluorescent probe. The genotype frequency and allele frequency and relative clinical datawere compared among groups. Results (1) BsmⅠ GG, GA, AA genotype frequencies were 0.931, 0.066, 0.003, allele G and allele G frequencies were 0.964 and 0.036. ApaⅠ CC、CA、AA genotype frequencies were0.536, 0.371, 0.093, allele C and allele A frequencies were 0.721 and 0.279. (2) ApaⅠ allele C frequencies (P<0.05) in DKD2 group (0.770) were significantly higher than in DKD0 group (0.671) , and the allele A frequencies (P<0.05) in DKD2 group (0.230) were significantly lower than in DKD0 group (0.329) (P<0.05) . (3) Logistic analysis showed that BsmⅠ GG genotype (OR = 0.159, P<0.05) was the independent protective factors of DKD occurrence, but it was not related to T2 DKD development. ApaⅠ single nucleotide polymorphism was not related to T2 DKD occurrence and development ( P >0.05) . 25 ( OH) D deficiency/insufficiency may b e the independent risk factor of T2 DKD development (OR = 1.957, P <0.05) .Conclusions BsmⅠ GG genotype is an independent protective factor of T2 DKDoccurrence, but it is not related to T2 DKD development.ApaⅠ singlenucleotide polymorphism is not related to T2 DKD occurrence and development.
2017, 38(08): 86-89.
Abstract:
Objective To investigate the clinical efficacy of interbody fusion with autogeneous bone in the treatment of lumbar spondylolisthesis. Methods 21 Cases of lumbar spondylolisthesis from January 2012 to June2016 were treated with interbody fusion with autogeneous bone. Before and after treatment, the imaging data, symptoms and signs, postoperative follow-up observation of vertebral reduction and vertebral bone graft fusion were observed and compared. Results In this study, 12 cases were excellent, 8 cases were better, 1 case was good; the excellent and good rate was 95.2%. Imaging results showed all the reduction of spondylolisthesis, the basic fusion of intervertebral bone graft 8 to 12 months after operation. The incidence of lumbar spondylolisthesis at 1 week, 3 months and 1 year after operation was significantly lower than that before operation (P<0.05) ;the intervertebral space height was significantly higher than that before operation (P <0.05) ; the angle of spondylolisthesis was significantly lower than that of preoperation (P <0.05) .Conclusion Interbody fusion with autogeneous bone in the treatment of lumbar spondylolisthesis is satisfactory, and worthy of promotion.
Objective To investigate the clinical efficacy of interbody fusion with autogeneous bone in the treatment of lumbar spondylolisthesis. Methods 21 Cases of lumbar spondylolisthesis from January 2012 to June2016 were treated with interbody fusion with autogeneous bone. Before and after treatment, the imaging data, symptoms and signs, postoperative follow-up observation of vertebral reduction and vertebral bone graft fusion were observed and compared. Results In this study, 12 cases were excellent, 8 cases were better, 1 case was good; the excellent and good rate was 95.2%. Imaging results showed all the reduction of spondylolisthesis, the basic fusion of intervertebral bone graft 8 to 12 months after operation. The incidence of lumbar spondylolisthesis at 1 week, 3 months and 1 year after operation was significantly lower than that before operation (P<0.05) ;the intervertebral space height was significantly higher than that before operation (P <0.05) ; the angle of spondylolisthesis was significantly lower than that of preoperation (P <0.05) .Conclusion Interbody fusion with autogeneous bone in the treatment of lumbar spondylolisthesis is satisfactory, and worthy of promotion.
2017, 38(08): 90-93.
Abstract:
Objective To study the improvement effect of ulinastatin for the oxygen free radical scavenging activity and inflammatory stress of patients with acute pancreatitis.Methods 52 patients with acute pancreatitis in our hospital from December 2015 to October 2016 were set as the observation object, and 52 patients were randomly divided into control group (26 cases) and observation group (26 cases) by the principle of random allocation. Patients in the control group were treated with routine treatment of acute pancreatitis, patients in the observation group were treated with ulinastatin on the basic treatment of control group.The oxygen free radical scavenging activity and inflammatory stress indexes of two groups before the treatment and at different time after the treatment were compared. Results The oxygen free radical scavenging activity and inflammatory stress indexes of control group and observation group before the treatment had no significant differences, all P >0.05, while the oxygen free radical scavenging activity and inflammatory stress indexes of observation group at different time after the treatment were all significantly better than the detection results of control group at the same time, all P<0.05, there were all significant differences. Conclusion The improvement effect of ulinastatin for the oxygen free radical scavenging activity and inflammatory stress of patients with acute pancreatitis is better, so the ulinastatin has active protection role for the body state of these patients.
Objective To study the improvement effect of ulinastatin for the oxygen free radical scavenging activity and inflammatory stress of patients with acute pancreatitis.Methods 52 patients with acute pancreatitis in our hospital from December 2015 to October 2016 were set as the observation object, and 52 patients were randomly divided into control group (26 cases) and observation group (26 cases) by the principle of random allocation. Patients in the control group were treated with routine treatment of acute pancreatitis, patients in the observation group were treated with ulinastatin on the basic treatment of control group.The oxygen free radical scavenging activity and inflammatory stress indexes of two groups before the treatment and at different time after the treatment were compared. Results The oxygen free radical scavenging activity and inflammatory stress indexes of control group and observation group before the treatment had no significant differences, all P >0.05, while the oxygen free radical scavenging activity and inflammatory stress indexes of observation group at different time after the treatment were all significantly better than the detection results of control group at the same time, all P<0.05, there were all significant differences. Conclusion The improvement effect of ulinastatin for the oxygen free radical scavenging activity and inflammatory stress of patients with acute pancreatitis is better, so the ulinastatin has active protection role for the body state of these patients.
2017, 38(08): 94-97.
Abstract:
Objective To analyze the present situation of hyponatremia in patients after traumatic brain injury and its risk factors. Methods 115 patients with traumatic brain injury in our hospital from February 2015 to December 2016 were selected as the study object, and the hyponatremia rate of all the patients was analyzed, and the hyponatremia rates of patients with different gender, ages, brain injury degree, ventricular hemorrhage, hydrocephalus, basal fracture, furosemide application and vasodilators application situation were compared, and the relationship between those factors and hyponatremia after traumatic brain injury were analyzed with Logistic regression analysis. Results 38 cases of 115 patients with traumatic brain injury were with hyponatremia, the rate was 33.04%, and the rates of patients with different gender and vasodilators application situation had no significant differences, all P>0.05, while the rates of patients with different ages, brain injury degree, ventricular hemorrhage, hydrocephalus, basal fracture and furosemide application situation had significant differences, all P <0.05, and the Logistic regression analysis showed that the ages, brain injury degree, ventricular hemorrhage, hydrocephalus, basal fracture and furosemide application were all the risk factors of hyponatremia present of these patients after traumatic brain injury. Conclusion The hyponatremia rate of patients after traumatic brain injury is high, and the age, brain injury degree, ventricular hemorrhage, hydrocephalus, basal fracture and furosemide application are all the risk factors, so they should be paid to intervention according to these risk factors.
Objective To analyze the present situation of hyponatremia in patients after traumatic brain injury and its risk factors. Methods 115 patients with traumatic brain injury in our hospital from February 2015 to December 2016 were selected as the study object, and the hyponatremia rate of all the patients was analyzed, and the hyponatremia rates of patients with different gender, ages, brain injury degree, ventricular hemorrhage, hydrocephalus, basal fracture, furosemide application and vasodilators application situation were compared, and the relationship between those factors and hyponatremia after traumatic brain injury were analyzed with Logistic regression analysis. Results 38 cases of 115 patients with traumatic brain injury were with hyponatremia, the rate was 33.04%, and the rates of patients with different gender and vasodilators application situation had no significant differences, all P>0.05, while the rates of patients with different ages, brain injury degree, ventricular hemorrhage, hydrocephalus, basal fracture and furosemide application situation had significant differences, all P <0.05, and the Logistic regression analysis showed that the ages, brain injury degree, ventricular hemorrhage, hydrocephalus, basal fracture and furosemide application were all the risk factors of hyponatremia present of these patients after traumatic brain injury. Conclusion The hyponatremia rate of patients after traumatic brain injury is high, and the age, brain injury degree, ventricular hemorrhage, hydrocephalus, basal fracture and furosemide application are all the risk factors, so they should be paid to intervention according to these risk factors.
2017, 38(08): 98-101.
Abstract:
Objective To study the effects of different fluorescent antibody dosages of HLA-B27 on the flow cytometry results in peripheral blood lymphocyte of ankylosing spondylitis (AS) .Methods The expression intensities of peripheral blood lymphocyte HLA-B27 were detected in 22 cases of AS and 30 cases of healthy individual by the flow cytometry. The anti-human FITC-HLA-B27/PE-CD3 antibody kit was divided into five groups (2 μL, 4 μL, 8 μL, 15 μL, 30 μL) . BD company set the value of fluorescence intensity≥146 as a positive result. Results The expression intensity in the healthy individuals were (55.97 ±6.44) , (59.90 ±7.15) , (67.97±7.62) , (81.60±6.63) , (91.97±5.77) , while in AS were (122.45±7.38) , (140.50±5.35) , (148.22±4.36) , (162.27±4.28) , (164.05±4.28) . The results rose along with the dosages of the antibody, the results in the healthy were negative. But in AS, if the dosages of antibody were more than 8 μL, the results were positive, and the results were similar in 15 μL and 30 μL, while if the dosages of antibody were less than 8 μL, the results were false negative (P <0.05) . Conclusion The 8μL dosage of antibody was helpful to decrease the miss diagnosis of AS.Simultaneously, with the increase of the amount of antibodies, the results do not have a substantial increase.
Objective To study the effects of different fluorescent antibody dosages of HLA-B27 on the flow cytometry results in peripheral blood lymphocyte of ankylosing spondylitis (AS) .Methods The expression intensities of peripheral blood lymphocyte HLA-B27 were detected in 22 cases of AS and 30 cases of healthy individual by the flow cytometry. The anti-human FITC-HLA-B27/PE-CD3 antibody kit was divided into five groups (2 μL, 4 μL, 8 μL, 15 μL, 30 μL) . BD company set the value of fluorescence intensity≥146 as a positive result. Results The expression intensity in the healthy individuals were (55.97 ±6.44) , (59.90 ±7.15) , (67.97±7.62) , (81.60±6.63) , (91.97±5.77) , while in AS were (122.45±7.38) , (140.50±5.35) , (148.22±4.36) , (162.27±4.28) , (164.05±4.28) . The results rose along with the dosages of the antibody, the results in the healthy were negative. But in AS, if the dosages of antibody were more than 8 μL, the results were positive, and the results were similar in 15 μL and 30 μL, while if the dosages of antibody were less than 8 μL, the results were false negative (P <0.05) . Conclusion The 8μL dosage of antibody was helpful to decrease the miss diagnosis of AS.Simultaneously, with the increase of the amount of antibodies, the results do not have a substantial increase.
2017, 38(08): 102-105.
Abstract:
Objective To investigate the effects of TSGF and HGF in CSF on ECM remodeling in glioma patients. Methods 83 cases of neurosurgery glioblastoma patients including grade Ⅰ 28 cases, 25 cases of patients with grade Ⅱ, Ⅲ grade 20 cases, Ⅳ grade 10 patients were enrolled. TSGF and EGF, MMP-2, TIMP-2, MMP-2/TIMP-2 were detected. Results TSGF and EGF in patients with grade Ⅱ compared with Ⅰappeared significantly higher level (P<0.05) , TSGF and EGF of Ⅲ grade patients compared with grade Ⅰ, Ⅱ grade were significantly higher (P<0.05) , TSGF and EGF of Ⅳ grade compared with gradeⅠ, Ⅱgrade, grade Ⅲ were significantly (P <0.05) . MMP-2, TIMP-2, MMP-2/TIMP-2 in grade Ⅱ patients was significantly different than that in grade Ⅰ (P <0.05) , MMP-2, TIMP-2, MMP-2/TIMP-2 in stage Ⅲpatients compared with Ⅰ, Ⅱ significantly different (P <0.05) , MMP-2, MMP-2/TIMP-2 in stage Ⅳcompared with grade Ⅰ, Ⅱ grade, grade Ⅲ showed significant difference (P <0.05) , TIMP-2 compared with grade Ⅰ, Ⅱ grade showed significant difference (P <0.05) . TSGF with MMP-2, MMP-2/TIMP-2showed significant positive correlation while showed negative correlation with TIMP-2 (P <0.05) , EGF and MMP-2, MMP-2/TIMP-2 showed significant positive correlation while showed negative correlation with TIMP-2 (P<0.05) . Conclusion Elevated cerebrospinal fluid levels of HGF and TSGF are an important factors leading to gliomas progress, the process of ECM remodeling-related factors are closely related to the progress, close monitoring those factors could help to make clear tumor progress.
Objective To investigate the effects of TSGF and HGF in CSF on ECM remodeling in glioma patients. Methods 83 cases of neurosurgery glioblastoma patients including grade Ⅰ 28 cases, 25 cases of patients with grade Ⅱ, Ⅲ grade 20 cases, Ⅳ grade 10 patients were enrolled. TSGF and EGF, MMP-2, TIMP-2, MMP-2/TIMP-2 were detected. Results TSGF and EGF in patients with grade Ⅱ compared with Ⅰappeared significantly higher level (P<0.05) , TSGF and EGF of Ⅲ grade patients compared with grade Ⅰ, Ⅱ grade were significantly higher (P<0.05) , TSGF and EGF of Ⅳ grade compared with gradeⅠ, Ⅱgrade, grade Ⅲ were significantly (P <0.05) . MMP-2, TIMP-2, MMP-2/TIMP-2 in grade Ⅱ patients was significantly different than that in grade Ⅰ (P <0.05) , MMP-2, TIMP-2, MMP-2/TIMP-2 in stage Ⅲpatients compared with Ⅰ, Ⅱ significantly different (P <0.05) , MMP-2, MMP-2/TIMP-2 in stage Ⅳcompared with grade Ⅰ, Ⅱ grade, grade Ⅲ showed significant difference (P <0.05) , TIMP-2 compared with grade Ⅰ, Ⅱ grade showed significant difference (P <0.05) . TSGF with MMP-2, MMP-2/TIMP-2showed significant positive correlation while showed negative correlation with TIMP-2 (P <0.05) , EGF and MMP-2, MMP-2/TIMP-2 showed significant positive correlation while showed negative correlation with TIMP-2 (P<0.05) . Conclusion Elevated cerebrospinal fluid levels of HGF and TSGF are an important factors leading to gliomas progress, the process of ECM remodeling-related factors are closely related to the progress, close monitoring those factors could help to make clear tumor progress.
2017, 38(08): 106-109.
Abstract:
Objective To investigate the correlation between POCT glucose meter and automatic biochemical analyzer test results, to evaluated the reliability of portable POCT blood glucose meter.Methods We vertically collected the blood glucose values that diabetics and non diabetics hospitalized in The First People's Hospital of Qujing of Endocrinology, from January 2012 to December 2015 regularly twice a year, the POCT blood glucose meter and Roche 7600 automatic analyzer test results, all of 448 cases, using SPSS17.0 for statistical analysis.Results The correlation of POCT blood glucose meter test results with Roche 7600 automatic analyzer was coefficiently, r > 0.95, meeting the standards of the Ministry of Public Health.Conclusions POCT glucose meter is simple, rapid, accurate and clinically useful. POCT glucose meter in our department, has good detection accuracy, stability and repeatability.
Objective To investigate the correlation between POCT glucose meter and automatic biochemical analyzer test results, to evaluated the reliability of portable POCT blood glucose meter.Methods We vertically collected the blood glucose values that diabetics and non diabetics hospitalized in The First People's Hospital of Qujing of Endocrinology, from January 2012 to December 2015 regularly twice a year, the POCT blood glucose meter and Roche 7600 automatic analyzer test results, all of 448 cases, using SPSS17.0 for statistical analysis.Results The correlation of POCT blood glucose meter test results with Roche 7600 automatic analyzer was coefficiently, r > 0.95, meeting the standards of the Ministry of Public Health.Conclusions POCT glucose meter is simple, rapid, accurate and clinically useful. POCT glucose meter in our department, has good detection accuracy, stability and repeatability.
2017, 38(08): 110-112.
Abstract:
Objective To observe the effect of hand function brace on post-stroke upper limb and hand dysfunction.Methods 65 cases of post-stroke hemiplegia dysfunction were randomly divided into two groups, the control group and the treatment group. The control group received routine treatment and basic medical treatment, the treatment group received hand function brace on the basis of the same treatment of control group.Before and 4 weeks after treatment respectively, the upper limb and hand function were assessed by simplified Fugl-Meyer motor function assessment and ADL.Results The Fugl-Meyer score after treatment, the treatment group was 39.2 + 2.7, 3.2 + 21.3 in the control group, the patients in the treatment group were significantly lower than the control group (P <0.05.ADL) the total effective rate after treatment, the treatment group91.18%, control group 67.74%. treatment group patients significantly lower than the control group (P<0.05) .Conclusion The hand function brace combined with occupational therapy can improve stroke hemiplegia Motor function and ADL in patients with upper limb and hand dysfunction.
Objective To observe the effect of hand function brace on post-stroke upper limb and hand dysfunction.Methods 65 cases of post-stroke hemiplegia dysfunction were randomly divided into two groups, the control group and the treatment group. The control group received routine treatment and basic medical treatment, the treatment group received hand function brace on the basis of the same treatment of control group.Before and 4 weeks after treatment respectively, the upper limb and hand function were assessed by simplified Fugl-Meyer motor function assessment and ADL.Results The Fugl-Meyer score after treatment, the treatment group was 39.2 + 2.7, 3.2 + 21.3 in the control group, the patients in the treatment group were significantly lower than the control group (P <0.05.ADL) the total effective rate after treatment, the treatment group91.18%, control group 67.74%. treatment group patients significantly lower than the control group (P<0.05) .Conclusion The hand function brace combined with occupational therapy can improve stroke hemiplegia Motor function and ADL in patients with upper limb and hand dysfunction.
2017, 38(08): 113-116.
Abstract:
Objective To investigate the curative effects of proximal femoral nail anti-rotation (PFNA) and dynamic hip system (DHS) on the unstable intertrochanteric fracture. Methods 64 cases of unstable intertrochanteric fracture patients were screened, and were randomly divided into the observation group and control group when admitted into hospital, and were respectively given proximal femoral nail anti-rotation and dynamic hip system treatment. The postoperative curative effects, complications incidences and hp functions in these two groups were investigated. Results There were 24 cases of one-stage healing in the observation group, 15 cases of one-stage healing in the control group; there were 7 cases of two-stage healing in the control group, 10 cases of two-stage healing in the observation group, there were significant differences between two groups (P<0.05) . The hip functions score in the observation group was (90.5 ± 6.7) points, control group (79.5 ±7.9) points, there was a significant difference between two groups (P<0.05) . The complications incidence in the observation was 3.1%, control group 25.0%, with significant difference, P <0.05. Conclusion The proximal femoral nail anti-rotation can improve better the condition and recover the hip function of patients with unstable intertrochanteric fracture.
Objective To investigate the curative effects of proximal femoral nail anti-rotation (PFNA) and dynamic hip system (DHS) on the unstable intertrochanteric fracture. Methods 64 cases of unstable intertrochanteric fracture patients were screened, and were randomly divided into the observation group and control group when admitted into hospital, and were respectively given proximal femoral nail anti-rotation and dynamic hip system treatment. The postoperative curative effects, complications incidences and hp functions in these two groups were investigated. Results There were 24 cases of one-stage healing in the observation group, 15 cases of one-stage healing in the control group; there were 7 cases of two-stage healing in the control group, 10 cases of two-stage healing in the observation group, there were significant differences between two groups (P<0.05) . The hip functions score in the observation group was (90.5 ± 6.7) points, control group (79.5 ±7.9) points, there was a significant difference between two groups (P<0.05) . The complications incidence in the observation was 3.1%, control group 25.0%, with significant difference, P <0.05. Conclusion The proximal femoral nail anti-rotation can improve better the condition and recover the hip function of patients with unstable intertrochanteric fracture.
2017, 38(08): 117-121.
Abstract:
Objective To investigate the status of stress disorder in patients with induced abortion and to analyze the influencing factors. Methods Abortion patients who received review (one month after surgery) or surgery (two years had a history of abortion) from department of Obstetrics and Gynecology in three medical institutions in Kunming from June 2016 to March 2017 were selected to conduct a questionnaire survey. The posttraumatic stress disorder scale (PCL-C) , hospital depression and anxiety scale (HADS) were used to measure the psychological status of the patients, application of spss 22.0 for statistical description and analysis.Results For 159 respondents, PCL-C score was 17-62 points, the average score (34.84 ±11.72) , PTSD positive detection of a total of 25 (15.7%) , Logistic regression analysis showed that Influencing factors of PTSD were marital status, account location, life satisfaction, trauma history, family economic status, anxiety status (P <0.05) .Conclusions The age of the abortion population is low. Induced abortion in patients with psychological difficulties, anxiety and depression are ubiquitous. Whether the increased risk of PTSD after induced abortion is still no consensus, traumatic history in this study may be a confounding factor, exaggerated the contribution of abortion to PTSD, to continue the relevant cohort study is necessary.
Objective To investigate the status of stress disorder in patients with induced abortion and to analyze the influencing factors. Methods Abortion patients who received review (one month after surgery) or surgery (two years had a history of abortion) from department of Obstetrics and Gynecology in three medical institutions in Kunming from June 2016 to March 2017 were selected to conduct a questionnaire survey. The posttraumatic stress disorder scale (PCL-C) , hospital depression and anxiety scale (HADS) were used to measure the psychological status of the patients, application of spss 22.0 for statistical description and analysis.Results For 159 respondents, PCL-C score was 17-62 points, the average score (34.84 ±11.72) , PTSD positive detection of a total of 25 (15.7%) , Logistic regression analysis showed that Influencing factors of PTSD were marital status, account location, life satisfaction, trauma history, family economic status, anxiety status (P <0.05) .Conclusions The age of the abortion population is low. Induced abortion in patients with psychological difficulties, anxiety and depression are ubiquitous. Whether the increased risk of PTSD after induced abortion is still no consensus, traumatic history in this study may be a confounding factor, exaggerated the contribution of abortion to PTSD, to continue the relevant cohort study is necessary.
2017, 38(08): 122-125.
Abstract:
Objective To understand about sources and ways of acquiring HIV/AIDS knowledge, willing to accept the ways of education and the demand situation among undergraduates from the different universities in Yunnan province, and provide scientific basis for making more effective health education strategy. Methods Using stratified random cluster sampling method, 1179 first and second year students from four different type universities (Kunming Medical University, Dali University, Yunnan Art Institute and Yunnan University) in different areas of Yunnan province ( Kunming and Dali) were selected and surveyed by anonymous questionnaire. Results HIV/AIDS knowledge of undergraduates from the different universities in Yunnan province came mainly from different schools (56.3% 80.3%) , followed by friends or partners (15.0 28.7%) .There were differences in the the same soure from four universities (P <0.05) . On channals to access that, the medical students accepted mainly through the classroom learning (39.0% 45.0%) , but students among arts and comprehensive universities tot mainly by the network or the news media (34.7% 36.2%) . There were differences in classroom teaching, news media and discussion from others (P <0.05) .The most willing to accept the way of HIV/AIDS health education among undergraduates was "watch the related video" of that (38.7% 62.0%) . There was on differen in being expected ways of doing things at watching video, knowledge lecture, classroom teaching and propaganda column column and leaflets (P > 0.05) among four universities.Undergraduates from four different universities hoped consistenly to carry out more special topic lecture (50.7%54.5%) . There were differences on demand of special topic lecture and classroom teaching (P <0.05) , which not different on demand of installing auto selling condom machine and propaganda column column and leaflets (P >0.05) from undergraduates from four different universitiess. Conclusions Although there are many differences among undergraduates from the different universities in Yunnan province, they have some sameness as well. According to different characteristics of their students and the actual situation in different universities, they should be targeted to carry out health education, and constantly improve undergraduates' cogntive level about HIV/AIDS knowledge in multi-ethnic frontier areas, which may help to form a correct attitude and behavior for them, then prevent and control the spread of HIV/AIDS.
Objective To understand about sources and ways of acquiring HIV/AIDS knowledge, willing to accept the ways of education and the demand situation among undergraduates from the different universities in Yunnan province, and provide scientific basis for making more effective health education strategy. Methods Using stratified random cluster sampling method, 1179 first and second year students from four different type universities (Kunming Medical University, Dali University, Yunnan Art Institute and Yunnan University) in different areas of Yunnan province ( Kunming and Dali) were selected and surveyed by anonymous questionnaire. Results HIV/AIDS knowledge of undergraduates from the different universities in Yunnan province came mainly from different schools (56.3% 80.3%) , followed by friends or partners (15.0 28.7%) .There were differences in the the same soure from four universities (P <0.05) . On channals to access that, the medical students accepted mainly through the classroom learning (39.0% 45.0%) , but students among arts and comprehensive universities tot mainly by the network or the news media (34.7% 36.2%) . There were differences in classroom teaching, news media and discussion from others (P <0.05) .The most willing to accept the way of HIV/AIDS health education among undergraduates was "watch the related video" of that (38.7% 62.0%) . There was on differen in being expected ways of doing things at watching video, knowledge lecture, classroom teaching and propaganda column column and leaflets (P > 0.05) among four universities.Undergraduates from four different universities hoped consistenly to carry out more special topic lecture (50.7%54.5%) . There were differences on demand of special topic lecture and classroom teaching (P <0.05) , which not different on demand of installing auto selling condom machine and propaganda column column and leaflets (P >0.05) from undergraduates from four different universitiess. Conclusions Although there are many differences among undergraduates from the different universities in Yunnan province, they have some sameness as well. According to different characteristics of their students and the actual situation in different universities, they should be targeted to carry out health education, and constantly improve undergraduates' cogntive level about HIV/AIDS knowledge in multi-ethnic frontier areas, which may help to form a correct attitude and behavior for them, then prevent and control the spread of HIV/AIDS.
2017, 38(08): 126-129.
Abstract:
EGFR mutations are closely associated with lung cancer development, this paper introduces the effectiveness of tyrosine kinase inhibitors on different epidermal growth factor receptor mutations and reviews the research progress of targeted drug resistance mechanisms, the aim is to further guide clinical targeted drugs.
EGFR mutations are closely associated with lung cancer development, this paper introduces the effectiveness of tyrosine kinase inhibitors on different epidermal growth factor receptor mutations and reviews the research progress of targeted drug resistance mechanisms, the aim is to further guide clinical targeted drugs.
2017, 38(08): 130-133.
Abstract:
Objective To improve the students' learning interest in tennis, promote the popularization and development of tennis, tennis skills and improve students' mental health. Methods Two Tennis Elective Classes of2013 Grade were selected, 57 people in one class, was as the experimental group, 58 people in and another class was as the control group. The experimental group used team learning mode in tennis teaching, the control group was given to the traditional teaching model. Results Two groups of students had significant differences in density, forehand and backhand (P<0.05) , there was significant difference between the scores of two factors of summarization and psychosis in (P <0.05) , there were significant differences in scores of two factors of interpersonal relationship and paranoia (P<0.01) . Conclusion The cooperative learning model can improve the density of exercise, has a good effect on improving tennis skills and mental health.
Objective To improve the students' learning interest in tennis, promote the popularization and development of tennis, tennis skills and improve students' mental health. Methods Two Tennis Elective Classes of2013 Grade were selected, 57 people in one class, was as the experimental group, 58 people in and another class was as the control group. The experimental group used team learning mode in tennis teaching, the control group was given to the traditional teaching model. Results Two groups of students had significant differences in density, forehand and backhand (P<0.05) , there was significant difference between the scores of two factors of summarization and psychosis in (P <0.05) , there were significant differences in scores of two factors of interpersonal relationship and paranoia (P<0.01) . Conclusion The cooperative learning model can improve the density of exercise, has a good effect on improving tennis skills and mental health.
2017, 38(08): 134-139.
Abstract:
Objective To develop transitional care model and test its effectiveness in a sample of patients receiving continuous ambulatory peritoneal dialysis (CAPD) . Methods From February 2013 to September2014, CAPD patients from nephrology department of the second affiliated hospital were randomly divided into two groups : the study group and the control group. The patients of study group were cared by the transitional care model during hospitalization and follow-up after 12 weeks of discharge while the control group were cared by the conventional care model. Patients were investigated with the KDQOL ( Kidney Disease Quality of Life) questionnaire in the day before discharge, then at week 6 and week 12 after discharge, respectively. The collected data were analyzed by the statistical software SPSS18.0 and the statistical methods included statistical description, t-test, chi-square test. Results The mean score of quality of life compared with study group and control group at the day before discharge, there were no statistically significant differences between two group (P>0.05) . At week 6, the study group had higher mean scores for quality of life than control group, there were statistically significant differences between two group (P <0.05) , however, there were statistically significant differences on physical health and symptom/problem in five dimensions at week 6 (P <0.05) . At week 12, the study group had significantly higher mean scores for quality of life than control group, there were statistically significant differences between two group (P <0.05) , nevertheless, there were no statistically significant differences on burden of kidney disease in five dimensions at week 12 (P>0.05) . Conclusions The transitional care model can improve CAPD patients' quality of life. It is a kind of emerging health care service pattern that could to maximize the health care benefits.
Objective To develop transitional care model and test its effectiveness in a sample of patients receiving continuous ambulatory peritoneal dialysis (CAPD) . Methods From February 2013 to September2014, CAPD patients from nephrology department of the second affiliated hospital were randomly divided into two groups : the study group and the control group. The patients of study group were cared by the transitional care model during hospitalization and follow-up after 12 weeks of discharge while the control group were cared by the conventional care model. Patients were investigated with the KDQOL ( Kidney Disease Quality of Life) questionnaire in the day before discharge, then at week 6 and week 12 after discharge, respectively. The collected data were analyzed by the statistical software SPSS18.0 and the statistical methods included statistical description, t-test, chi-square test. Results The mean score of quality of life compared with study group and control group at the day before discharge, there were no statistically significant differences between two group (P>0.05) . At week 6, the study group had higher mean scores for quality of life than control group, there were statistically significant differences between two group (P <0.05) , however, there were statistically significant differences on physical health and symptom/problem in five dimensions at week 6 (P <0.05) . At week 12, the study group had significantly higher mean scores for quality of life than control group, there were statistically significant differences between two group (P <0.05) , nevertheless, there were no statistically significant differences on burden of kidney disease in five dimensions at week 12 (P>0.05) . Conclusions The transitional care model can improve CAPD patients' quality of life. It is a kind of emerging health care service pattern that could to maximize the health care benefits.
2017, 38(08): 140-143.
Abstract:
Objective To compare the effects of different disinfection methods on skin disinfection effects in blood sampling, and to find a rapidly applicable method which is easy to operate and suitable for sampling of bloodculture. Methods We randomly sampled 303 patients in a 3A Grade Hospital from February 2016 to May2016, and chose three parts, the fossa of left and right elbow and the cubitus of right arm, from every patient.Then those patients were divided into three groups according to skin disinfection methods used before blood sampling. The first group underwent the disinfection method of blood sampling stipulated by America Clinical Laboratory Standards Institute (CLSI) ;the second group was applied on Anerdian clockwise for one time;the third group was applied on Anerdian both clockwise and counter-clockwise each once. A skin swab on the same spot of every group was conducted before and after the disinfection for bacterial culture and colony counting, which was statistically analyzed by χ2test. Results The difference of positive rate was statistically significant among three disinfection methods before and after disinfection (P1 <0.01; P2 <0.01; P3 <0.01) . No statistical significance was found in the difference between CLSI disinfection and both clockwise and counter-clockwise each once of Anerdian disinfection (P >0.05) ; statistical significance was found in the difference between CLSI disinfection and clockwise Anerdian disinfection once for each time (P <0.01) ; and there was statistical significance in the difference between both clockwise and counter-clockwise and clockwise Anerdian disinfection once for each time ( P <0.01) .Conclusions The effect of clockwise and counter-clockwise Anerdian disinfection once for each time is equivalent to that of the CLSI disinfection method for blood culture and sampling. Anerdian can be applied on skin disinfection for blood culture specimen via venous sampling, and it is more easy, rapid and economic to operate.
Objective To compare the effects of different disinfection methods on skin disinfection effects in blood sampling, and to find a rapidly applicable method which is easy to operate and suitable for sampling of bloodculture. Methods We randomly sampled 303 patients in a 3A Grade Hospital from February 2016 to May2016, and chose three parts, the fossa of left and right elbow and the cubitus of right arm, from every patient.Then those patients were divided into three groups according to skin disinfection methods used before blood sampling. The first group underwent the disinfection method of blood sampling stipulated by America Clinical Laboratory Standards Institute (CLSI) ;the second group was applied on Anerdian clockwise for one time;the third group was applied on Anerdian both clockwise and counter-clockwise each once. A skin swab on the same spot of every group was conducted before and after the disinfection for bacterial culture and colony counting, which was statistically analyzed by χ2test. Results The difference of positive rate was statistically significant among three disinfection methods before and after disinfection (P1 <0.01; P2 <0.01; P3 <0.01) . No statistical significance was found in the difference between CLSI disinfection and both clockwise and counter-clockwise each once of Anerdian disinfection (P >0.05) ; statistical significance was found in the difference between CLSI disinfection and clockwise Anerdian disinfection once for each time (P <0.01) ; and there was statistical significance in the difference between both clockwise and counter-clockwise and clockwise Anerdian disinfection once for each time ( P <0.01) .Conclusions The effect of clockwise and counter-clockwise Anerdian disinfection once for each time is equivalent to that of the CLSI disinfection method for blood culture and sampling. Anerdian can be applied on skin disinfection for blood culture specimen via venous sampling, and it is more easy, rapid and economic to operate.
2017, 38(08): 144-148.
Abstract:
Objective To explore the impact on the patients' satisfaction of the nurses by using ISBAR standard communication tool in patients receiving hemodialysis at high risk of falling. Methods We collected150 cases of hospitalized patients with hemodialysis from December 2015 to February 2016. Using computer random number method in advance, 150 patients were randomly divided into experimental group (n = 74) and the control group (n = 76) . Patients in the experimental group used ISBAR communication tool for shifting, patients in the control group were given regular shifting. Results One week after intervention, the scores of the satisfaction on the nurse of patients in experimental group was (72.2 + 4.5) , in the control group was (70.1 +6.4) , there was a statistically significant difference between two groups (P < 0. 05) . The total scores of the satisfaction on the nurse of patients from admission to discharge in the experimental group was (81.6 + 2.1) , in the control group was (77.5 + 5.0) , there was a statistically significant difference between two groups (P <0.001) . The falls management assessment score of nurse increased from (87.4±4.1) to (93.1±2.8) (P <0. 05) . Conclusion ISBAR communication tool can improve nursing quality in hemodialysis patients at high risk of falling.
Objective To explore the impact on the patients' satisfaction of the nurses by using ISBAR standard communication tool in patients receiving hemodialysis at high risk of falling. Methods We collected150 cases of hospitalized patients with hemodialysis from December 2015 to February 2016. Using computer random number method in advance, 150 patients were randomly divided into experimental group (n = 74) and the control group (n = 76) . Patients in the experimental group used ISBAR communication tool for shifting, patients in the control group were given regular shifting. Results One week after intervention, the scores of the satisfaction on the nurse of patients in experimental group was (72.2 + 4.5) , in the control group was (70.1 +6.4) , there was a statistically significant difference between two groups (P < 0. 05) . The total scores of the satisfaction on the nurse of patients from admission to discharge in the experimental group was (81.6 + 2.1) , in the control group was (77.5 + 5.0) , there was a statistically significant difference between two groups (P <0.001) . The falls management assessment score of nurse increased from (87.4±4.1) to (93.1±2.8) (P <0. 05) . Conclusion ISBAR communication tool can improve nursing quality in hemodialysis patients at high risk of falling.
